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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Camtaro420
Birdman



Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 6 months
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Re: Bacteria and tupperware containers [Re: twistedty]
#19299111 - 12/19/13 05:36 PM (10 years, 2 months ago) |
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Quote:
twistedty said:
Quote:
anne halonium said: ill imitate you and prosper. thanx for the tip. 

with your advise, im sure i can get more than 4 flush jars. these were done on nylon poms.........
glad you set me straight. i was thinking my yield could be like yours!
why do you grow in lil cups
They look like they are cracking from lack of RH.
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CMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 9 months
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Re: Bacteria and tupperware containers [Re: Camtaro420]
#19299131 - 12/19/13 05:40 PM (10 years, 2 months ago) |
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Been gone for a couple months, glad to see there is still bickering going on between a few of the regulars. Fun stuff.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Bacteria and tupperware containers [Re: anne halonium]
#19299180 - 12/19/13 05:51 PM (10 years, 2 months ago) |
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pints?
those pics were pry taken before most of you were even growing. cubes are soooo 1990 anyway.
we have phased out cubes last few yrs. we now grow the rare chicon nindos mexicanas more power fruits. plus stones.

in cargo case FC's

and under LED blue 450 nm with PP5 v tek style.

you can see more of these rare specimens, in capt futures thread.
http://www.shroomery.org/forums/showflat.php/Number/16262797
kinda rare species. very few can grow em.
lemme know when your ready to upgrade. youll need a stronger skill set to do these to scale
once again, my lab has been essentially all plastic, for about a decade now. its not the plastics, its your handling, and possibly your tek.
now, allow me to advise the OP in a serious and experienced way. if the OP REALLY thinks its the plastics, soak em overnight under 10% bleach first. make sure they are immersed 100%
you can be lab sure , your cool at that point.
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Edited by anne halonium (12/19/13 07:03 PM)
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Scarg
Self Defecator


Registered: 10/17/11
Posts: 153
Last seen: 2 years, 2 months
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Re: Bacteria and tupperware containers [Re: anne halonium]
#19299751 - 12/19/13 08:04 PM (10 years, 2 months ago) |
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The point is that it worked really really well up until a certain point. That point also happened to be the same time that the containers were being used for the second or third time. Im not saying its not the spawn. It very well could be, and ive already got some fresh stuff from spores on agar. But none of the jars smelled or looked funky..so it stands to reason that my crappy old scrubber (which is definitely full of bacteria) didnt do a good job of washing them.
Im trying an experiment with the same spawn in a few brand new containers, a couple of containers that were washed and pcd for 30 mins, and a couple that were just washed with the scrubber-no pc.
Somebody mentioned that the micropore tape during colonization is unnessecary..is that true?
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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Re: Bacteria and tupperware containers [Re: Scarg]
#19299779 - 12/19/13 08:09 PM (10 years, 2 months ago) |
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Quote:
Scarg said: The point is that it worked really really well up until a certain point. That point also happened to be the same time that the containers were being used for the second or third time. Im not saying its not the spawn. It very well could be, and ive already got some fresh stuff from spores on agar. But none of the jars smelled or looked funky..so it stands to reason that my crappy old scrubber (which is definitely full of bacteria) didnt do a good job of washing them.
Im trying an experiment with the same spawn in a few brand new containers, a couple of containers that were washed and pcd for 30 mins, and a couple that were just washed with the scrubber-no pc.
Somebody mentioned that the micropore tape during colonization is unnessecary..is that true?
if you put anything in the pc for sterilization in a container you dont need to wash it
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Bacteria and tupperware containers [Re: twistedty]
#19299891 - 12/19/13 08:33 PM (10 years, 2 months ago) |
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we use pp5 containers and loose lids for FAE, so we have no need for micropore or lid mods really.

bacteria , can be persistant sometimes. we know by law they should die under certain conditions. now explain that to the bacteria. they arent always hot on the latest memo.
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Edited by anne halonium (12/19/13 09:06 PM)
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 10 years, 22 days
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Re: Bacteria and tupperware containers [Re: anne halonium]
#19301496 - 12/20/13 05:57 AM (10 years, 2 months ago) |
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I think you have a problem with your bulk sub...
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Scarg
Self Defecator


Registered: 10/17/11
Posts: 153
Last seen: 2 years, 2 months
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Re: Bacteria and tupperware containers [Re: Nakor420]
#19304997 - 12/20/13 08:22 PM (10 years, 2 months ago) |
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Just contemplating how it might be the spawn thats the problem. Ive had MS culture going since early September. I started from print->agar->jar>g2g 10jars...and from there spawned some jars, and g2g'd more...and so on and so on. So its been about 3 months now.
Im fairly confident with my sterile technique, and having the flowhood has really put my success rate way up. So yes, im still considering that the problem is improper cleaning of the tupperwares..but......
Is it possible that the culture is just worn out due to senescence, and is simply more susceptible to bacterial contamination?
How old is too old for viable spawn?
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crackacola
Big Daddy


Registered: 12/14/13
Posts: 234
Last seen: 8 years, 10 months
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Re: Bacteria and tupperware containers [Re: Scarg]
#19306131 - 12/21/13 02:19 AM (10 years, 2 months ago) |
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Dude. Quit using a sponge/scrubber for your own personal benefit. Use a washcloth. They even make washcloths w/ a scrubby side. Wash that shit after you use it. No sense in keeping dirty ass sponges/scrubbers other than maybe an SOS pad. I'm with you on that. It very well could be your sponge.
Its probably in your spawn though. Your substrate is verily contam resistant. How many generations is your G2G? You only want to go a couple generations in before starting a new run. Otherwise stuff like this happens too. Just start all over and ditch the scrubby. I bet you'll be fine again.
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FOR TRADE <---- Check out my spores for trade!
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