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OfflineRandomFX
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Registered: 12/02/13
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Update on my growing, welcome critiques or confirmations
    #19299023 - 12/19/13 05:21 PM (10 years, 1 month ago)

I am using the PF tek to make spawn, in pint, half pint and qaurt jars. seems to work fine and is easy enough. I have just started using saw dust from wood pellets instead of BRF/verm, because I got 40 lb bags of 100% hardwood pellets for $1.99 ea, so bought 20 of them for $40, but those are to new to report on yet, though I plan on using that more as a substrate when I have more developed spawn. however I am using plugs I got from ebay in them and not Liquid Culture. I've thought about using the plugs in honey/water etc to make liquid culture, but I haven't seen anyone explain if there was actually logic in doing it. anyways I tried boiling cut straw to pasteurize and that grew the mycelium fairly well, but it got contaminated and had to throw it out. I've seen in a few posts that you need a pressure cooker? do not have one yet unfortunately.
I also tried using hydrogen peroxide in a 1 H2O2 to 9 parts H20, and the mycelium seemed to not want to spread well on that, however, after a 2-3 day delay it did and it is doing well.
I also took wood ash, which I have plenty of, and soaked cut straw for 16 hours, drained it, and the mycelium didn't spread that well, like the Hydrogen peroxide, but then picked up, and nice white fluffy mycelium, non contaminated. So far, I haven't went to fruiting stage on anything, but the mycelium smells wonderful.

I get I shouldn't probably be using the plugs, but it is what I have at the moment. The variety I am using is grey oyster and golden oyster.

So the questions I am and would like peoples thoughts on are should I try to put a plug or two in honey water to make a liquid culture? (Am I right that is making a liquid culture?) should I do something else entirely? I can guess it would probably be 'better' to actually buy liquid culture, but once again this is what I have. I do not yet understand how important it is or its relative value yet as compared to using the plugs. though I know I will get into using agar, petri dishes, my microscope, making a flow hood/etc. soon surely. anyways thanks, half the fun is talking to others about stuff...I know most of you are way past this stuff by now, and it's probably a bit worn out.


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Offlinet3chnobily
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Re: Update on my growing, welcome critiques or confirmations [Re: RandomFX]
    #19299558 - 12/19/13 07:22 PM (10 years, 1 month ago)

Welcome to the Shroomery! 

You got a long way to go and you will grow a mountain of moldy substrates but stick with it and mushrooms will come.

Plugs are not ideal for culture but they can work.  Buy grain spawn next time round.

For oysters your best bet to start is synthetic straw logs.

Search for hot water and lime bath pasteurization.  Pick one that works for you.

Do you have a PC and a SAB for sterile culture?

Think about a FC.  Bins and marthas don't have enough FAE for oysters.  Depending on your winter temps try a hoop house or mini green house.  If too cold look for basement or garage space.

Stick with it.  Not that hard just a lot of new info all at once.


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OfflineRandomFX
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Re: Update on my growing, welcome critiques or confirmations [Re: t3chnobily]
    #19300334 - 12/19/13 10:17 PM (10 years, 1 month ago)

thanks. you are saying a bin nor a martha has enough Free Air Exchange? even if I hook up 1 or maybe 2 computer case fans? Case fans con go up above 100 cfm, or down to whatever you want really. 100 cubic ft/minute 'sounds' like a lot of airflow to me, how much should I have? I was even thinking of setting up a external flowbox to catch any spores/etc, but not sure yet.

and you think I should buy grain spawn? isn't that highly expensive compared to spawning on your own grain? or is doing that, bad for a reason I do not know about?

I do not have a pressure cooker yet, I do plan on getting one, I can make a Still Air Box, I have a full woodworking garage.

and I am confused by you asking about thinking about getting a fruiting chamber. isn't that what a bin and a martha is used for? or is there a different, or better 'fruiting chamber' that I do not know about? like something specific?

thanks for the help, I will stick with it...heck I'm in already, no sense stopping now.


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Offlinethiotimoline
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Registered: 12/01/12
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Re: Update on my growing, welcome critiques or confirmations [Re: RandomFX]
    #19303045 - 12/20/13 01:54 PM (10 years, 1 month ago)

Oysters need a lot more fresh air than most other mushrooms. I fruit mine in open air, misting once or twice a day, and it works fine, though if you're in a desert things will be different.

Plugs are specifically for inoculating stumps or logs. For straw or sawdust or any medium where you can mix the spawn uniformly, it helps a lot to have spawn that's in small pieces like grains, so that colonization starts from a huge number of points at once and the mycelium has less distance to travel. The faster the substrate colonizes, the less chance contaminants have to take hold.


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OfflineRandomFX
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Re: Update on my growing, welcome critiques or confirmations [Re: thiotimoline]
    #19308171 - 12/21/13 02:00 PM (10 years, 1 month ago)

are you worried about spores causing health problems or do you have a area that can handle all the spores from oysters fruiting in the open? I have heard/read it can be a real problem, but of course have no experience myself. It is winter here, which gets to 5 F and so will be fruiting in a non occupied basement room, but still am leery. I do not have real air filtration yet, probably will eventually though, but I want to make this worth it first. honestly though do not think that will be a issue, so far my mycelium is by everything I can see, doing excellent. Relative humity in my home right now is unfortunately 40 though. and the room I am doing this in I cannot saturate, or do not want to, it sort of needs (I think?) to be in a enclosed environment of some type.
I just this morning actually took two blue tubs, inverted one over the other, drilled 1/4' holes in the bottom one with a 4" hole for a computer fan which is speed adjustable, cut a small slit in the top to fit the t5 light I have, and will be sealing off with a plexiglass strip (So humidity in the chamber doesn't trash the light)and keeping the light on the outside to shine in the chamber.

This is only temporary of course, the fan and humidifier and light will all be on timers, and I plan on doing the same setup but with a martha/mini greenhouse so far when I get it. I am excited actually. I have one block of grey oyster inoculated straw that I am getting ready to attempt to fruit. It is probably about 80% covered in mycelium right now, and in a ziplock bag with holes and spore tape covering the holes. I am debating on removing the spore tape to allow Fresh air exchange to maybe get it to fruit through the holes, or removing it from the bag altogether....but when I think about it, I see people grow in buckets with holes drilled in them ok...so am not seeing why I would NEED to remove it from the plastic at all? What does everyone think?


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