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OfflineScarg
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Bacteria and tupperware containers
    #19298459 - 12/19/13 03:19 PM (10 years, 3 months ago)

Recently Ive been having some fantastic success colonizing my pasteurized sub in tupperware containers.  I tested out 6 to see how they would go..they worked, so I kept buying more and more. Success, success, success!

Here we are a couple of cycles later, and Im having huge issues with bacteria.  I work in front of a flowhood and my grain jars have all smelled perfect before I used them. 

So im wondering if maybe my washing method could be causing this?  As far as I can tell, the brand new containers all worked like a charm, but its after theyve been washed with soap and water with a dish scrubber, that theyve started failing.  Now that I think about it..that scrubber probably has bacteria all over it..but soap and water seems like its all clean and rinsed away.

Going to buy another small pack of tupperwares and see if they do alright.  In the meantime, anybody have any ideas on what else it might be?  Or an effective way to clean the tupperwares?

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OfflineSagescruffy
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Re: Bacteria and tupperware containers [Re: Scarg]
    #19298467 - 12/19/13 03:20 PM (10 years, 3 months ago)

Have you considered cleaning them with boiling water?


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Love.

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OfflineCamtaro420
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Re: Bacteria and tupperware containers [Re: Scarg]
    #19298474 - 12/19/13 03:22 PM (10 years, 3 months ago)

Soap and water alone could never clean your containers well enough. It's impressive they did not contaminate from the packaging.

You need to get PP5 labled containers and lids (very cheap and most commonly found these days) if you are looking to re-use your containers because these can withstand steamers and pressure cookers.


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InvisibleSpitballJedi
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Re: Bacteria and tupperware containers [Re: Scarg]
    #19298520 - 12/19/13 03:31 PM (10 years, 3 months ago)

It's not likely your containers or the washing. Washing out tubs with soap and hot water is standard practice.

What is your substrate recipe and pasteurization tek?

What is your spawn and sterilization tek?


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OfflineScarg
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Re: Bacteria and tupperware containers [Re: SpitballJedi]
    #19298581 - 12/19/13 03:46 PM (10 years, 3 months ago)

Definitely not my pasteurization tek..ive got that shit nailed down.  I use franks coir/verm/gypsum to the T.  Havent done it any different from the LOADS of success to now.  Spawn is rye grain prepped to RR's prep..except I PC for two hours every time.  Im almost certain its the containers.  Having to pressure cook the empty container seems excessive.  I thought soap and water was standard practice too...works for monotubs.  I might go with a bleach hot water bath. But if its gotta be a PC cycle..ill try that too.  How long you think I should cook for?

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InvisibleSpitballJedi
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Re: Bacteria and tupperware containers [Re: Scarg]
    #19298617 - 12/19/13 03:57 PM (10 years, 3 months ago)

Your spawn can easily have a contamination that's gone unnoticed. It can happen when using MS syringes because they are not likely to be 100% clean, could be your jar filters, could be your inoculation technique. could be a problem with your flowhood.

Properly pasteurized CVG is very contam resistant. The source is most likely the spawn. The question is "how is your spawn getting contaminated?"


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OfflineNakor420
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Re: Bacteria and tupperware containers [Re: Sagescruffy]
    #19298645 - 12/19/13 04:08 PM (10 years, 3 months ago)

Quote:

Sagescruffy said:
Have you considered cleaning them with boiling water?



Yeah...fill them with boiling water and let them sit for a while with the lid on...are you using all your bulk sub at one time or are you storing it and using it in increments for smaller scale grows?


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OfflineGoldenArrow
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Re: Bacteria and tupperware containers [Re: SpitballJedi]
    #19298653 - 12/19/13 04:10 PM (10 years, 3 months ago)

If you're PCing the containers with the rye in it should kill anything in there....so it'd be a problem either with the seal or the inoculation process.

..or your grains are too wet now.

Edited by GoldenArrow (12/19/13 04:12 PM)

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OfflineScarg
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Re: Bacteria and tupperware containers [Re: GoldenArrow]
    #19298783 - 12/19/13 04:37 PM (10 years, 3 months ago)

Quote:

GoldenArrow said:
If you're PCing the containers with the rye in it should kill anything in there....so it'd be a problem either with the seal or the inoculation process.

..or your grains are too wet now.





Nope.  Pcing grain jars.  Transferring fully colonized, musroom smelly jars to properly pasteurized sub.  Putting the mixture in the tupperwares.

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OfflineNakor420
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Re: Bacteria and tupperware containers [Re: Scarg]
    #19298835 - 12/19/13 04:48 PM (10 years, 3 months ago)

Are you sealing it tight with the Tupperware lid?


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Edited by Nakor420 (12/19/13 04:50 PM)

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OfflineScarg
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Re: Bacteria and tupperware containers [Re: Nakor420]
    #19298864 - 12/19/13 04:54 PM (10 years, 3 months ago)

Quote:

Nakor420 said:
Are you sealing it tight with the Tupperware lid?




Ive been using the lids, but have drilled 5 pin sized holes (4 corners and the centre)..and covered them with fresh micropore tape each time.  Like i said, the first cycle on every container worked beautifully..fully colonized in 6 days or so.  Im going to try the pc.  At 15psi, 30 mins should work ya?

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Invisibleanne halonium
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Re: Bacteria and tupperware containers [Re: Scarg]
    #19298877 - 12/19/13 04:56 PM (10 years, 3 months ago)

has nothing to do with plastics.
your introducing bacteria/ contams in the handling chain.


pastuerization works when done RIGHT.
and right, is not defined by mostly right, and hope for the best.

peeps set up to pasturize, make a big mess spreading shit all over.
then they stand back and pasturize, and then pull the stuff out, and handle it unclean cultivator and all,same enviorment
re introducing contams.

years ago, i watched a  noob mix a bulk with a wood spoon in a dirty room, then pasturize, then put the spoon back in the bulk.
then he wondered why he had trich.

no one ever teaches bio handling, or the nuances of contams.
they just tell ya to boil it up some way, recipe style.
sometimes it works, sometimes it doesnt.

peeps have built carreers here on such shoddy handling advise.
after all, anytime one of these recipe tek fails,
it had to be the spores! ( or the devil of the day..plastics")

contams, and handling, really arent witchcraft............
plastics arent spoiling your day.
your handling chain, and possibly your tek is.


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:aliendance:

Edited by anne halonium (12/19/13 05:04 PM)

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InvisibleSpitballJedi
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Re: Bacteria and tupperware containers [Re: Scarg]
    #19298894 - 12/19/13 05:00 PM (10 years, 3 months ago)

There's no point to taping the holes while colonizing substrate.

Your sponge and washing method would have to pretty damn shitty for it to be causing your contamination. It's your spawn.


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Invisible36fuckin5
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Re: Bacteria and tupperware containers [Re: anne halonium]
    #19298928 - 12/19/13 05:06 PM (10 years, 3 months ago)

Spores won't germinate on pasteurized substrates for at leasta few weeks, and bacteria isn't a problem in your bulk.

Anne, just keep your methods to yourself. Nobody follows your bullshit anyway. Grow your mushrooms the way you grow them and sell them for the exorbitant prices you do. Keep supporting cartels and paying for child labor in 3rd world countries.

Show me one single pic of an actual healthy mushroom (not some shriveled up crap with a tiny, thin cap because of your LEDs) and I may give you a little credit. Otherwise just stop annoying everyone.

And learn proper fucking English. You're old enough to have figured it out how to use punctuation and commas properly now. Your shit is extremely annoying to read.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Invisibleanne halonium
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Re: Bacteria and tupperware containers [Re: 36fuckin5]
    #19298954 - 12/19/13 05:11 PM (10 years, 3 months ago)

Quote:

36fuckin5 said:


Show me one single pic of an actual healthy mushroom (not some shriveled up crap with a tiny, thin cap because of your LEDs) and I may give you a little credit. Otherwise just stop annoying everyone.

extremely annoying to read.




your just sorta confused on bio handling,
so of course your annoyed.



next.

Edited by anne halonium (12/19/13 05:15 PM)

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Invisibleanne halonium
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Re: Bacteria and tupperware containers [Re: anne halonium]
    #19298966 - 12/19/13 05:13 PM (10 years, 3 months ago)

oops......double post.


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:aliendance:

Edited by anne halonium (12/19/13 05:14 PM)

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Invisible36fuckin5
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Re: Bacteria and tupperware containers [Re: anne halonium]
    #19298984 - 12/19/13 05:15 PM (10 years, 3 months ago)

No, I'm just confused as to why you call yourself an expert when every pic you've ever shown has not had one healthy mushroom in it.

Nothing personal against you, I enjoy talking to you when you're not spouting all your bullshit. I already ran the numbers, in the 3 month cycle you use, I can double your output getting only one flush per tub, and yes it's at a decent scale.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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Re: Bacteria and tupperware containers [Re: 36fuckin5]
    #19298998 - 12/19/13 05:17 PM (10 years, 3 months ago)

That mushroom is lacking FAE. You can tell by the small cap and the thin stem. Also, I've seen it before. Not impressed.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Invisibleanne halonium
jaguarette
Female


Registered: 05/07/13
Posts: 1,908
Re: Bacteria and tupperware containers [Re: 36fuckin5]
    #19299016 - 12/19/13 05:19 PM (10 years, 3 months ago)

ill imitate you and prosper.
thanx for the tip. :thumbup:



with your advise, im sure i can get more than 4 flush jars.
these were done on nylon poms.........

glad you set me straight.
i was thinking my yield could be like yours!


--------------------
:aliendance:

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Offlinetwistedty
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Re: Bacteria and tupperware containers [Re: anne halonium]
    #19299096 - 12/19/13 05:34 PM (10 years, 3 months ago)

Quote:

anne halonium said:
ill imitate you and prosper.
thanx for the tip. :thumbup:



with your advise, im sure i can get more than 4 flush jars.
these were done on nylon poms.........

glad you set me straight.
i was thinking my yield could be like yours!




why do you grow in lil cups

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