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OfflineGod Stamet
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Registered: 07/01/12
Posts: 774
Last seen: 9 years, 2 months
cube storage.
    #19298432 - 12/19/13 03:11 PM (10 years, 1 month ago)

hey guys,

just wondering what the proper storage temp is for cubes. I've heard as high as 50F. my slants have medical tongue depressors and are kept in an insulated lunch box. I keep my fridge a little warmer then usual but nowhere near 50F. I'm going to buy a mini fridge for my cultures and I just want to make sure I have it optimized.


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I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK


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OfflineRogerRabbitM
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Re: cube storage. [Re: God Stamet]
    #19302373 - 12/20/13 11:13 AM (10 years, 1 month ago)

45 to 50 is OK.  I lost a bunch of cultures at 35F.
RR


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Download Let's Grow Mushrooms



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OfflineGod Stamet
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Re: cube storage. [Re: RogerRabbit]
    #19302697 - 12/20/13 12:25 PM (10 years, 1 month ago)

thanks RR. I definitely don't like the recovery of most of the MYC harvested after long term storage in a near 40F environment. I have to be picky with my transfers and that costs precious time.


--------------------
I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK


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OfflineAmanita virosa
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Re: cube storage. [Re: RogerRabbit]
    #19304765 - 12/20/13 07:39 PM (10 years, 1 month ago)

Quote:

RogerRabbit said:
45 to 50 is OK.  I lost a bunch of cultures at 35F.
RR



Same here thanks to shitty microfridge storage!  If you can afford a fridge that curculates the air with fans aka wine fridge it's well worth it for keeping the cultures at a constant temp. Micro fridges suck for that. They can be  45 degrees one day and 35 the next. Unfortunately the fancy ones are pricy.


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OfflineGod Stamet
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Registered: 07/01/12
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Re: cube storage. [Re: Amanita virosa]
    #19306939 - 12/21/13 08:45 AM (10 years, 1 month ago)

Quote:

Amanita virosa said:
Quote:

RogerRabbit said:
45 to 50 is OK.  I lost a bunch of cultures at 35F.
RR



Same here thanks to shitty microfridge storage!  If you can afford a fridge that curculates the air with fans aka wine fridge it's well worth it for keeping the cultures at a constant temp. Micro fridges suck for that. They can be  45 degrees one day and 35 the next. Unfortunately the fancy ones are pricy.





great tip. I was just going to buy a simple mini fridge. pricy or not, I need something that works consistently


--------------------
I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK


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Invisiblefastfred
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Re: cube storage. [Re: RogerRabbit]
    #19310155 - 12/22/13 12:59 AM (10 years, 1 month ago)

Quote:

RogerRabbit said:
45 to 50 is OK.  I lost a bunch of cultures at 35F.
RR




Fridges don't have that great of temp control.  The cultures probably repeatedly froze and thawed until they were mush.

Standard lab storage for cultures is usually 4C (39F).

If you're serious about a good storage fridge then get a cheap Chinese PID temp. controller.  Last I checked they were around $35.  You can wire them into your fridge in place of the mechanical thermostat and place the temp probe right near your cultures.


-FF


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It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

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I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


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OfflineAmanita virosa
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Re: cube storage. [Re: fastfred]
    #19313667 - 12/22/13 09:00 PM (10 years, 1 month ago)

The ones with fans that run 24/7 work great. They are designed for chilling beverages and have excellent thermostats in them.  Thy are bout twice the price of a cheap micro fridge though.


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OfflineGod Stamet
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Re: cube storage. [Re: Amanita virosa]
    #19313712 - 12/22/13 09:10 PM (10 years, 1 month ago)

Quote:

Amanita virosa said:
The ones with fans that run 24/7 work great. They are designed for chilling beverages and have excellent thermostats in them.  Thy are bout twice the price of a cheap micro fridge though.




I have been doing research and looking for the best bang for my buck since your first post. thanks a million for that great tip AV!!


--------------------
I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK


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Invisiblefastfred
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Re: cube storage. [Re: God Stamet]
    #19313763 - 12/22/13 09:25 PM (10 years, 1 month ago)

Looks like PID temp. controllers are about $14 now...
search.ebay.com/PID-Digital-Temperature

Seems like a pretty good price to me!  If I had some spare cash I'd do all my fridges.


-FF


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OfflineAmanita virosa
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Re: cube storage. [Re: fastfred]
    #19313796 - 12/22/13 09:35 PM (10 years, 1 month ago)

Quote:

fastfred said:
Looks like PID temp. controllers are about $14 now...
search.ebay.com/PID-Digital-Temperature

Seems like a pretty good price to me!  If I had some spare cash I'd do all my fridges.


-FF



How complicated is that sucker to wire into a freezer to convert to fridge.  Can you explain it a bit FF.


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OfflineGod Stamet
wannabe Stamets


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Re: cube storage. [Re: Amanita virosa]
    #19313824 - 12/22/13 09:42 PM (10 years, 1 month ago)

ya I clicked that link and tilted my head like a confused dog lol.


--------------------
I have caught the Mycology bug! Not just here for the ride, i'm here for life.

How I do my bulk substrate

just another mushroom capsule TEK


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Offlinedrake89
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Re: cube storage. [Re: God Stamet]
    #19318707 - 12/23/13 09:56 PM (10 years, 1 month ago)

well I can tell you my Johnson A941 or whatever it is, I'm not too happy with.  i've got one rigged up to a heater for my ghetto rigged incubation room, and the wiring doesn't make any sense.  you've got to use a jumper that seems like it should be included.  but regardless my heater quit working, not sure why. 

so for now it's on a recycling timer while i'm on vaycay


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Invisiblefastfred
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Re: cube storage. [Re: Amanita virosa]
    #19319637 - 12/24/13 03:52 AM (10 years, 1 month ago)

Quote:

Amanita virosa said:
How complicated is that sucker to wire into a freezer to convert to fridge.  Can you explain it a bit FF.




It's pretty easy to do.  In your fridge you'll find a copper tube or bulb looking thing.  That is the temp sensor.  Follow the copper wire back to the mechanical thermostat.  This is what you'll be replacing.

The bulb/temp. sensor inside the fridge is filled with fluid, it connects to the mechanical thermostat with senses the pressure of the fluid and turns the compressor on and off.

The mechanical thermostat just works like a switch.  All you have to do is remove it and attach the connections to the relay of the PID controller.  Then put the digital temp probe somewhere in the fridge.

On some systems the thermostat is right in the fridge near the temp. probe.  In others the probe tube runs back to a thermostat near the compressor.

Mounting and making the whole thing look slick is up to you, but not usually that difficult.  The easiest way would probably be to put the controller on top and just run wires down to the thermostat connections down by the compressor, then drill a hole in it to put the probe in.  But there's probably nicer ways to do it.


-FF


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OfflineAmanita virosa
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Re: cube storage. [Re: fastfred]
    #19319945 - 12/24/13 07:29 AM (10 years, 1 month ago)

Excellent!  Thanks. We are trying to keep fresh mush out of our store cooler as our biggest lab contam ends up being pleurotus spores.  Lab is on the same level seperated by two doors.  If I can convert a couple of chest freezers over to fridge temp I can use the big stand alone commercial beverage cooler in the store just for spawn and still keep my mush chilled for market and wholesale.


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