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thebileball
Stranger


Registered: 11/18/13
Posts: 12
Last seen: 6 years, 9 months
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how long will mycelium continue to colonize new spawn?
#19298302 - 12/19/13 02:40 PM (10 years, 2 months ago) |
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How long can mycelium continue to colonize new substrates(without allowing it to fruit)?
In TMC there is a diagram of taking ten grain jars, inoculating them, and then splitting each of those ten times once fully colonized.
Is there a difference between using that process with horse manure or other spawn? For example, will the mycelium ever get "tired" of colonizing without fruiting?
THANKS!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: how long will mycelium continue to colonize new spawn? [Re: thebileball]
#19298308 - 12/19/13 02:43 PM (10 years, 2 months ago) |
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Yes it will search senscene, it can only go through so many cell divisions
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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thebileball
Stranger


Registered: 11/18/13
Posts: 12
Last seen: 6 years, 9 months
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Re: how long will mycelium continue to colonize new spawn? [Re: cronicr]
#19298353 - 12/19/13 02:55 PM (10 years, 2 months ago) |
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what?
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Camtaro420
Birdman



Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 6 months
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Re: how long will mycelium continue to colonize new spawn? [Re: cronicr]
#19298361 - 12/19/13 02:57 PM (10 years, 2 months ago) |
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Quote:
cronicr said: Yes it will, search senscene, it can only go through so many cell divisions
Fix'd it for you to understand OP.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: how long will mycelium continue to colonize new spawn? [Re: Camtaro420]
#19298374 - 12/19/13 02:59 PM (10 years, 2 months ago) |
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Thnx bud i hate my phone lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Camtaro420
Birdman



Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 6 months
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Re: how long will mycelium continue to colonize new spawn? [Re: cronicr]
#19298379 - 12/19/13 03:00 PM (10 years, 2 months ago) |
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Quote:
cronicr said: Thnx bud i hate my phone lol
Word. Figured it was something like that. I hate posting from mine too. =(
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thebileball
Stranger


Registered: 11/18/13
Posts: 12
Last seen: 6 years, 9 months
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Re: how long will mycelium continue to colonize new spawn? [Re: Camtaro420]
#19298383 - 12/19/13 03:01 PM (10 years, 2 months ago) |
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what does senscene mean?
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thebileball
Stranger


Registered: 11/18/13
Posts: 12
Last seen: 6 years, 9 months
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Re: how long will mycelium continue to colonize new spawn? [Re: Camtaro420]
#19298400 - 12/19/13 03:04 PM (10 years, 2 months ago) |
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senescence* thank you
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: how long will mycelium continue to colonize new spawn? [Re: thebileball]
#19298421 - 12/19/13 03:09 PM (10 years, 2 months ago) |
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Mycelium grows through cell division like you and i and can only do it x amount of times before it basicly dies
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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Re: how long will mycelium continue to colonize new spawn? [Re: thebileball]
#19298426 - 12/19/13 03:10 PM (10 years, 2 months ago) |
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Most people will do 3-5 grain to grain tranfers (G2G's) max before going back to a master jar.
You would spawn grains to horse manure, but you wouldn't usually use horse manure as a spawn. But G2G's are something best left until you have a lot of experience with sterile procedure. But even a noob could spawn BRF cakes to a coir 50/50 or a Horse manure/straw substrate. Making that only 1 single transfer.
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thebileball
Stranger


Registered: 11/18/13
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Last seen: 6 years, 9 months
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Re: how long will mycelium continue to colonize new spawn? [Re: vikingsc]
#19298445 - 12/19/13 03:16 PM (10 years, 2 months ago) |
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cool. so what's the big deal with using horse manure as spawn?
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 10 years, 23 days
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Re: how long will mycelium continue to colonize new spawn? [Re: thebileball]
#19298682 - 12/19/13 04:18 PM (10 years, 2 months ago) |
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Quote:
thebileball said: cool. so what's the big deal with using horse manure as spawn?
You don't really NEED manure unless you are using a species that requires a very high nitrogen source. such as pans
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Stool_Sample
Fruity



Registered: 09/25/12
Posts: 223
Loc: North East, US
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Re: how long will mycelium continue to colonize new spawn? [Re: thebileball]
#19298687 - 12/19/13 04:19 PM (10 years, 2 months ago) |
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Quote:
thebileball said: How long can mycelium continue to colonize new substrates(without allowing it to fruit)?
In TMC there is a diagram of taking ten grain jars, inoculating them, and then splitting each of those ten times once fully colonized.
Is there a difference between using that process with horse manure or other spawn? For example, will the mycelium ever get "tired" of colonizing without fruiting?
From my perspective, the simplest reason is Grain can be broken apart easily and transferred from one Container to another. Manure will clump and require much more effort to brake and transfer.
Edited by Stool_Sample (12/19/13 04:36 PM)
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 10 years, 23 days
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Re: how long will mycelium continue to colonize new spawn? [Re: Stool_Sample]
#19298691 - 12/19/13 04:21 PM (10 years, 2 months ago) |
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I think he meant manure as bulk sub...
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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Re: how long will mycelium continue to colonize new spawn? [Re: Nakor420]
#19299193 - 12/19/13 05:55 PM (10 years, 2 months ago) |
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Grain is far more nutritious for one and colonize much quicker and far more nutrients in a quart jar of grain than a quart of horse manure. Spawn needs to be sterile a substrate doesn't, it can be pastuerized. Most only use horse manure as bulk substrate rather than putting it in jars and inoculating it, but it is possible- spawn bags more likely.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: how long will mycelium continue to colonize new spawn? [Re: cronicr]
#19299213 - 12/19/13 05:59 PM (10 years, 2 months ago) |
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Quote:
thebileball said: what does senscene mean?
Quote:
cronicr said: Yes it will, search senscene, it can only go through so many cell divisions
senescence is how it's spelled by the way.
^also what they said^
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thebileball
Stranger


Registered: 11/18/13
Posts: 12
Last seen: 6 years, 9 months
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Re: how long will mycelium continue to colonize new spawn? [Re: vikingsc]
#19324408 - 12/25/13 04:40 AM (10 years, 2 months ago) |
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so if someone just wanted to be lazy and not use a flow hood would it be really terrible to only use manure after your first grain transfer? or, this seems to good to be true but noc up manure and just keep building trays? thats kinda like spawn bags right?
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