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Offlinesinkingstone
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Myc / Clone / 'generations' questions
    #19296742 - 12/19/13 06:23 AM (10 years, 1 month ago)

Hi everyone! 
First,  Thanks for a great community and an amazing forum.
I'm Sinkingstone, student of life, from the SouthEast USA. Trying my hand at this for the first time since 1993-94.  Thanks for having me.

I'm having a little trouble viewing some pages because I don't have enough posts.  I try hard to search out all answers and not contribute to the redundant database but I need the post count so here are a couple things I've been trying to figure out... (again, the search engine told me to make 3 more posts, so yes I did use the search feature:)

I spawned some brf cakes to  bulk trays of hpoo/straw. Fruiting now, all good.

While breaking up the cakes to spawn, I set aside two chunks of myc and added it to two other 1/2pint containers of hpoo/straw/coffee.  These are contam free and almost 100% colonized.

Is this still 1st generation?

How long will this myc keep going if I keep colonizing to 100%, breaking it up and spawning to more substrate?

I'm a very experienced hydroponics grower (20yrs), and keeping this myc going is similar to keeping a plant in vegetative growth stages and cloning it frequently. My experience is that a plant will procuce great clones for years if kept vegetative.  I just don't know how the myc will perform.

Any insight?

Thanks!


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InvisibleFooMan
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Re: Myc / Clone / 'generations' questions [Re: sinkingstone]
    #19296822 - 12/19/13 06:59 AM (10 years, 1 month ago)

You can go a few generations like that, but the mycelium will get weaker over time and with each transfer, making it more suceptible to contaminants.

If you want a master first generation culture, you should isolate a culture on agar from spores. Once you find a culture with the traits you want you would create a master culture slant which you could use to start future grows with.

There are a bunch of threads already dealing with isolating and creating culture slants so I won't go into detail on that. The search feature is your friend :thumbup:


--------------------

Quick WBS Prep


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Offlinesinkingstone
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Re: Myc / Clone / 'generations' questions [Re: FooMan]
    #19301539 - 12/20/13 06:18 AM (10 years, 1 month ago)

Makes sense, I'm getting the hang of it,  there are a LOT of variables involved obviously.  I'm going to look further into isolating, I still have a lot of studying to do about spore prints and it looks like fruit will be ready soon.

It's been superb watching/transferring myc as I've been able to watch it grow closely and apply a visual to what I've read.

So based on this info, I have a 2nd generation and a 3rd generation tray of bulk almost ready to fruit. I will fruit them side by side and take note.

Thanks everyone!


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Invisiblesoicyboy97
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Re: Myc / Clone / 'generations' questions *DELETED* [Re: sinkingstone]
    #19302730 - 12/20/13 12:34 PM (10 years, 1 month ago)

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Offlinesinkingstone
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Re: Myc / Clone / 'generations' questions [Re: soicyboy97]
    #19303906 - 12/20/13 05:01 PM (10 years, 1 month ago)

Gotcha.  so I would have to start another colony from one of the two 2nd gen 1/2-pints to have gen #3.  Haha. I'm getting there. Won't be going through all that though.


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OfflinePussyFart
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Re: Myc / Clone / 'generations' questions [Re: sinkingstone]
    #19303949 - 12/20/13 05:11 PM (10 years, 1 month ago)

Quote:

sinkingstone said:
I spawned some brf cakes to  bulk trays of hpoo/straw. Fruiting now, all good.

While breaking up the cakes to spawn, I set aside two chunks of myc and added it to two other 1/2pint containers of hpoo/straw/coffee.  These are contam free and almost 100% colonized.

Is this still 1st generation?



Yes.....you only get to another generation by cloning.

If you clone a fruit, then grow it out and fruit it, those would be second generation fruits...if you clone one of those, grow it out and fruit it that would be gen3, and so on.

A G2G transfer/spawning is not a generation, neither is a second or third flush.

Quote:

sinkingstone said:
How long will this myc keep going if I keep colonizing to 100%, breaking it up and spawning to more substrate?



Not long.....spawning bulk substrate to bulk substrate is called super spawning and it is not recomended.

Quote:

soicyboy97 said:
according to your original post you should have one tray that is 1st gen, and 2 half pints that are 2nd gen



Not true.....I read nothing about him cloning or taking tissue from anything....

Quote:

sinkingstone said:
Gotcha.  so I would have to start another colony from one of the two 2nd gen 1/2-pints to have gen #3.



No.....an expansion of mycellium is NOT a generation....no matter how many times it is expanded.


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Invisiblesoicyboy97
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Re: Myc / Clone / 'generations' questions *DELETED* [Re: PussyFart]
    #19304524 - 12/20/13 06:58 PM (10 years, 1 month ago)

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Offlinesinkingstone
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Re: Myc / Clone / 'generations' questions [Re: soicyboy97]
    #19304896 - 12/20/13 08:04 PM (10 years, 1 month ago)

That all makes sense. this is all going to potentially produce first generation fruit, but the expanded myc may be degraded in comparison to the original jars.


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OfflinePussyFart
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Re: Myc / Clone / 'generations' questions [Re: soicyboy97]
    #19304922 - 12/20/13 08:09 PM (10 years, 1 month ago)

Quote:

soicyboy97 said:
well then my point is that u name the transfers 1 2 3 4 and they get progressively weak from transfer to transfer...so u do not wanna exceed to many transfers



Quote:

sinkingstone said:
That all makes sense. this is all going to potentially produce first generation fruit, but the expanded myc may be degraded in comparison to the original jars.



Wait, are we talking about g2g transfer or bulk sub to bulk sub transfers?


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Offlinesinkingstone
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Re: Myc / Clone / 'generations' questions [Re: PussyFart]
    #19304987 - 12/20/13 08:22 PM (10 years, 1 month ago)

Bulk to bulk essentially. I'm working with smallish amounts of hpoo.  I was considering a continuous process of colonizing hpoo and using that to colonize more.

The original 'jars' were brf, spawned to hpoo. I used a few chunks of brf cake to colonize some extra 1/2-pint size bulk sub containers and was unclear on the potential to spawn those extra bulk subs to more bulk sub. Also I was considering a chunk of myc a clone.  Sorry, still learning.


Edited by sinkingstone (12/20/13 08:29 PM)


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