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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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First time mad bag grow with cambodian strain (puncture) HELP!
#19296264 - 12/19/13 02:09 AM (10 years, 1 month ago) |
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Hey everyone! I'll start by saying much reading and researching has been done prior to diving into this but no matter what one reads it doesn't compare to actually having everything in front of you ready to go, please be understanding.
Decisions were made and a mad bag and Cambodian spores were obtained.
alcohol wiped surgical gloves on - sterilization measures were taken via spray bottle w/ diluted bleach in distilled water area was covered with plastic sterilized w/ the bleach. all equipment (plastic tub, temp & hydrometer gauges, scalpel, etc.) was sterilized.
***mad bag was also sprayed w/ the sterilization fluid and substrate was kneaded to ensure it was loose. Using the scalpel to slice a small hole to open the plastic covering on the injection port the scalpel slipped and caused a small puncture next to the injection site, this caused MUCH ALARM! and The site was immediately wiped with alcohol.***
using new surgical gloves wiped with alcohol and face mask the the outside of the bag containing the syringe was sterilized. Spore syringe was removed from it's zip-lock and immediately wiped with alcohol then flamed and inserted into the recommended injection port inoculating in 3 spots* left side, right side and center (better safe than sorry) with approx 1/2cc in each site.
after removing the syringe from injecting it was wiped with alcohol and flamed cap restored and the whole thing was wiped with alcohol and placed back in the zip-lock with a quick spritz of the bleach solution and (later) placed in the fridge.
Mad bag injection site was wiped with alcohol again along with accidental puncture site and placed in a sterilized zip-lock bag the bag was not sealed so that there could be some restricted airflow without exposing the puncture site. then it was placed inside the sterilized plastic tub and everything was again sprayed with the bleach solution and the lid was put on. that went into two(2) black garbage bags so that the first garbage bag could be sprayed and then sealed by going into the second and then tied loosely and sprayed.
being stored at 74-80 degrees f with 80-90% humidity
hoping that the hole will not ruin this grow as log as sterilization precautions are maintained throughout process.
As this is the first grow expectations are at a minimum just having fun with it and enjoying the learning experience.
once the mycelium is fully cultivated it's planned to remove the substrate and place it in a pyrex sheet in the tub to allow maximum fruiting so any advice on this would be appreciated along with anything in order to ensure protection during beginning stages.(that hole in the bag is a major stress factor! Thanks! I hope this wasn't too long but I wanted everything covered as best as possible to avoid unneeded questions.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19296334 - 12/19/13 02:36 AM (10 years, 1 month ago) |
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i would have just taped it with micropore tape
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: cronicr]
#19296368 - 12/19/13 02:52 AM (10 years, 1 month ago) |
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Thought about it but was unsure about sterility. I know it's hypoallergenic, just worried about things clinging to the adhesives on the tape
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19296373 - 12/19/13 02:53 AM (10 years, 1 month ago) |
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Do it in a sab
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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daltonvz
Stranger



Registered: 11/15/13
Posts: 104
Loc: North Dakota
Last seen: 9 years, 9 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19296387 - 12/19/13 02:58 AM (10 years, 1 month ago) |
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My friend..you are going through way to much cleaning than is necessary. You are growing mushrooms from mycelium which is fungi. You are not performing open heart surgery. Yes some careful cleaning should be done, but DAMN!
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: cronicr]
#19296390 - 12/19/13 03:02 AM (10 years, 1 month ago) |
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very good, thanks! Should it be ok as is until the sab is ready? Maybe the day after tomorrow the longest 3 days? have to get a suitable container.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19296394 - 12/19/13 03:03 AM (10 years, 1 month ago) |
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Should be fine
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
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Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: daltonvz]
#19296401 - 12/19/13 03:08 AM (10 years, 1 month ago) |
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haha yeah, well everything everywhere I've read emphasizes sterilization and I know with pf tek there is a vermiculite layer to prevent contam. with the bag there is no protection except well, the bag... so once that got punctured the precautions were raised. lol It does seem a little ridiculous though.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19296720 - 12/19/13 06:13 AM (10 years, 1 month ago) |
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Quote:
error37invaliduser said: Using the scalpel to slice a small hole to open the plastic covering on the injection port
What compelled you to do something silly like that? You inject through the plastic covering the port.
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Quick WBS Prep
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: FooMan]
#19297623 - 12/19/13 11:33 AM (10 years, 1 month ago) |
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well injecting through the plastic film was planned but the syringe came with a blunted tip on it not a spike so I didn't have much of a choice. Didn't want to risk sharpening the blunt tip and contaminating.
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daltonvz
Stranger



Registered: 11/15/13
Posts: 104
Loc: North Dakota
Last seen: 9 years, 9 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19300344 - 12/19/13 10:19 PM (10 years, 1 month ago) |
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Most of them are blunt that are big.
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 5 months, 20 days
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: daltonvz]
#19300563 - 12/19/13 11:28 PM (10 years, 1 month ago) |
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Just to be sure........ You never mentioned anything about pressure cooking your substrate or what your substrate is for that matter?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: vikingsc]
#19300569 - 12/19/13 11:30 PM (10 years, 1 month ago) |
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Because he didnt pressure cook it.....the company did
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: vikingsc]
#19303212 - 12/20/13 02:32 PM (10 years, 1 month ago) |
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a pre-sterilized myco bag from one of the sponsor vendors was used. An sab was built and the puncture in question has been sealed so hopefully all works out. I'll update along the way thanks for the help cronicr . Next time pftek will be the way to go, it seems way more cost effective and the variables can be controlled better.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19303878 - 12/20/13 04:54 PM (10 years, 1 month ago) |
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Quote:
error37invaliduser said: well injecting through the plastic film was planned but the syringe came with a blunted tip on it not a spike so I didn't have much of a choice. Didn't want to risk sharpening the blunt tip and contaminating.
Then you had the wrong equipment and shouldn't have proceeded. Syringe tips are cheap and plentiful. Nobody is gonna ask any questions for you buying a 1.5" 16 gauge needle. Nobody is sticking that in their arm. Maybe in their horse.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: 36fuckin5]
#19304428 - 12/20/13 06:37 PM (10 years, 1 month ago) |
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^ this is true.
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19346462 - 12/30/13 02:06 PM (10 years, 1 month ago) |
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first signs of colonization found today, white blotches scattered over 30% of substrate. it's been about 2 weeks since inoculation and 3 days since last checked.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: error37invaliduser]
#19346475 - 12/30/13 02:08 PM (10 years, 1 month ago) |
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 glad to hear
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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error37invaliduser
Lawn Boy



Registered: 12/03/13
Posts: 258
Loc: obscured by clouds
Last seen: 3 years, 8 months
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Re: First time mad bag grow with cambodian strain (puncture) HELP! [Re: cronicr]
#19457627 - 01/22/14 01:31 PM (10 years, 8 days ago) |
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about 90% colonized, gonna give it a little over a week to consolidate. I feel like it took a long time to get to this point, but I've heard that is one of the downsides to the bags(long colonization period) Def. not fruiting in the bag. Thinking of putting into a sgfc or monotub opinions/advice I've read positives both ways.
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