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brianthorekjr
Stranger

Registered: 12/16/13
Posts: 5
Last seen: 10 years, 1 month
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dead spores?
#19285543 - 12/16/13 07:23 PM (10 years, 2 months ago) |
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dead spores I inoculated my jars three weeks ago is it possible that my spores are dead I can see them in the syringe .temp at 80 did half with the alcohol sponge tek and half with a flowbench I have had good luck before but it seems like these are dead is there anyway to make agar with products at home .how long should you wait after heating the needle .please help
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MachineElf1.618
4-PO-Dimethyltryptamine



Registered: 06/27/11
Posts: 1,911
Loc: Miðgarðr
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Are you doing cakes or grain jar? Pics wold help too
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brianthorekjr
Stranger

Registered: 12/16/13
Posts: 5
Last seen: 10 years, 1 month
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unique dung and popcorn tek
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Search for the brf agar sub and thats a horrible way to go
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: dead spores? [Re: cronicr]
#19290545 - 12/17/13 08:48 PM (10 years, 2 months ago) |
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Quote:
cronicr said: Search for the brf agar sub and thats a horrible way to go

I was thinking about a cotton candy and boogers sub, might be fun.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Or if u got agar click pastys link. Spores dont like to germ on poo
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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brianthorekjr
Stranger

Registered: 12/16/13
Posts: 5
Last seen: 10 years, 1 month
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Re: dead spores? [Re: cronicr]
#19292960 - 12/18/13 11:46 AM (10 years, 2 months ago) |
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ok so germination on poo is prob not going to happen I guess I will wait for my popcorn jars which show signs of germanation
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brianthorekjr
Stranger

Registered: 12/16/13
Posts: 5
Last seen: 10 years, 1 month
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Re: dead spores? [Re: cronicr]
#19295854 - 12/18/13 11:53 PM (10 years, 2 months ago) |
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can I sterilize an empty syringe and suck up some sterile brown rice flower and inoculate the poo
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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wildernessjunkie
Reshitivest



Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: dead spores? [Re: cronicr]
#19295882 - 12/18/13 11:58 PM (10 years, 2 months ago) |
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Quote:
can I sterilize an empty syringe and suck up some sterile brown rice flower and inoculate the poo
No.
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chowster
Happy Farmer

Registered: 11/20/13
Posts: 182
Last seen: 2 years, 11 months
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Re: dead spores? [Re: cronicr]
#19295887 - 12/19/13 12:00 AM (10 years, 2 months ago) |
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Quote:
cronicr said:
 
lol
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Quote:
brianthorekjr said: can I sterilize an empty syringe and suck up some sterile brown rice flower and inoculate the poo
ok i think i get you now, do u mean colonize it like the brf agar sub, then take sterile water in a syringe squirt it in your agar sub, suck it up and inoculate?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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brianthorekjr
Stranger

Registered: 12/16/13
Posts: 5
Last seen: 10 years, 1 month
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Re: dead spores? [Re: cronicr]
#19300942 - 12/20/13 01:36 AM (10 years, 2 months ago) |
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popcorn is doing good going to wait till full colonized take one jar suck up like ten cc of sterile water blow it in to the jar and then suck it out I think that will work.i have a five pound bag of poo one big superjar of poo 6 small popcorn jars and one 1 pound popcorn bag and a big popcorn jar when one of the small ones colonize I will use one of them they are like halfway now thanks for the info about the poo .it just seemed like a good idea and is if you have a culture they come pre sterilized and are guaranteed.should have got the berries .wanted to stay away from the pf tek only get a 60 percent success rate and that's with a flow hood.I am such a fucken newb.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Your not gonna get ten cc's back,the grains will absorb some..just letting u know
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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