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OfflineJohn in WI
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Registered: 12/18/13
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Hello, and a few noobie questions
    #19294076 - 12/18/13 04:30 PM (10 years, 1 month ago)

Hello Folks, I have spent a lot of time lurking here, and doing TONS of reading.  Excellent resource, I have to say.

I fiddled around with growing p. cubensis back in college using the traditional agar plate method.  I honestly didn't have much luck as my pressure cooker leaked very badly, and my junky stove could barely get it hot enough to do 5 or 6psi. 

I dropped the idea for about 15 years, and some time ago thought, why not try again?  I have a few bucks for better equipment and an actual house to work out of. 

So I ordered some p. cubensis B+ and they threw in a Malabar syringe.  My substrate was pretty simple.  50% brown rice (whole, pre-cooked), 50% rye flour/vermiculite, with a couple handfuls of quinoa and a couple of whole flax seed.  It looks good--very moist, but super airy and no standing water in the jars.  I capped them with about 1cm of dry verm, and pressure cooked an honest 14psi for an honest hour.  I saw some folks who only put two holes in the dome, so I did that (out of laziness).

So my observations so far is that after inoculating about .25cc of solution into each hole, 16 out of 16 of the B+ showed signs of life.  That was on day 5.  It's now nearly day 8 and the Malabar is barely showing anything.  Last I looked, only 4 of the inoculation sites showed anything (out out 32 holes in the dome).  I don't think I cooked the spores as I cooled the substrate overnight.  So I don't know if the Malabar is just slower, and might take a bit or what.  Anyone have experience with it? I think it might  be just a little bit chilly, and I'm about to head out and get some kind of safe heater--maybe one of those oil filled electric radiators for the space.

Anyway, how long do you guys wait before you get worried or give up?  I'm patient, but it seemed weird that one variety would spring to life, and the other seems sluggish.


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Offlinesilverstem
Caps & Stems
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Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 6 years, 5 months
Re: Hello, and a few noobie questions [Re: John in WI]
    #19294096 - 12/18/13 04:33 PM (10 years, 1 month ago)

8 days is not a long enough wait for ms... i would start to worry at about week 3.


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InvisibleLazarus.Long
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Registered: 09/10/13
Posts: 83
Loc: World as Myth
Re: Hello, and a few noobie questions [Re: silverstem]
    #19294135 - 12/18/13 04:41 PM (10 years, 1 month ago)

:whathesaid:


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