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OfflineCogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
Black spots in LC
    #19292009 - 12/18/13 06:38 AM (10 years, 1 month ago)

Hi there, I recently inoculated a liquid culture using a Dutch king spore syringe,
After incubation I saw a couple black spots inside the mycelium strands that were floating in the culture, I used the solution to inoculate a couple "test" BRF cakes and surely enough within a couple days I have thick mycelial growth with 0% contam..
In light of this I just put the black spots down as ungerminated spores that had been suffocated by the living mycelium.. So I put the culture in the fridge, for longer term storage..

Anyway, I took the culture out today just to give it an eyeballin'  and see what's happening with it... When I took it outta the fridge I noticed almost instantly that there were a lot more of these black spots than that of before... Wich to me confirms that my initial assumption "that the spots were ungerminated spores" is in fact incorrect... I mean how could there suddenly be more ungerminated spores..?

So I suppose my question is, what the hell are these things?



I'm sorry for the terrible quality of the pictures :') and before I get any response let me just say that as a long time reader and first time poster to this site, you've made mushroom cultivation possible for me, and for that I will forever be grateful :wink:


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great write-up for new-guys :smile:
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Cogsy


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Black spots in LC [Re: Cogsy]
    #19292021 - 12/18/13 06:44 AM (10 years, 1 month ago)

Probably contams, my friend. Sorry for being the bearer of bad news, but LC's are sketchy as hell at best. :frown:


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Offlinedceodhz
Up And Coming

Registered: 10/19/13
Posts: 78
Loc: That's NO Moon
Last seen: 6 years, 3 months
Re: Black spots in LC [Re: Pestile]
    #19292027 - 12/18/13 06:47 AM (10 years, 1 month ago)

LC is definitely sketchy, especially from MS, I'm still toying with the idea of isolate to LC but G2G from my Masters is just so easy


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OfflineCogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
Re: Black spots in LC [Re: Pestile]
    #19292037 - 12/18/13 06:52 AM (10 years, 1 month ago)

That's what my gut is telling me pestilence... But I can't see how it's a contam if the spots were already there when I tested on the BRF cakes and got 0 contam... Possibly straight up luck?


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great write-up for new-guys :smile:
___________________________________________________________


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Cogsy


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Offlinetga8706
Stranger
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Registered: 10/31/13
Posts: 157
Last seen: 8 years, 9 months
Re: Black spots in LC [Re: Cogsy]
    #19292059 - 12/18/13 07:03 AM (10 years, 1 month ago)

Before you inoculated the jar did you have any particles or chunks that settled to the bottom? I made some LC jars with dry malt extract and they all had settlement at the bottom. That's where the mycelium grew first engulfing the particles at the bottom. That's what it looks like to me especially since you tested it already. They just look darker as the mycelium engulfs it more.


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OfflineCogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
Re: Black spots in LC [Re: tga8706]
    #19292096 - 12/18/13 07:24 AM (10 years, 1 month ago)

They did all settle at the bottom, I was kinda worried about that at the time and was hugely relieved when they went into suspension... And what you said about it getting darker, that is probably the case... I'd gone a few days without looking at it, so I probably mistook the increased darkness for more spots :P


--------------------
great write-up for new-guys :smile:
___________________________________________________________


____________________________________________________________

Cogsy


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OfflineCogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
Re: Black spots in LC [Re: Cogsy]
    #19292119 - 12/18/13 07:33 AM (10 years, 1 month ago)

Just as a precaution I've taken a syringe from the first LC and used that to inoculate another fresh LC and this will hopefully colonise without any black bits :laugh:


--------------------
great write-up for new-guys :smile:
___________________________________________________________


____________________________________________________________

Cogsy


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