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Zeros
Hey it's 420



Registered: 11/18/12
Posts: 79
Last seen: 3 years, 3 months
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Rye colonization question
#19289476 - 12/17/13 04:48 PM (10 years, 1 month ago) |
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I've been growing mushrooms for about a year now and understand growing conditions and sterile procedure but I can't figure out why my rye won't colonize. I've used multiple syringes from a reputable vendor, waited for the grains to cool and inoculated. The grains were soaked for a few days, rinsed and pressure cooked at 15 psi for 90 minutes. I have an old pressure cooker and it may be slightly over cooking the grains because they come out much darker than pre cook. They also don't shake well at all. The water content inside of the jar leaves saturation marks where the grains touch the glass but no excess water at the bottom. The only other thing I can think of is the temperature of the room they are in. Its warm maybe 80-85 degrees. Last batch 4 jars colonized after maybe 2 months and the rest did nothing. I have 6 jars now that are at about 18 days after inoculation with no results. Any input would be awesome.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Rye colonization question [Re: Zeros]
#19289498 - 12/17/13 04:54 PM (10 years, 1 month ago) |
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Quote:
Zeros said: The grains were soaked for a few days,
They also don't shake well at all.

they probably started fermentation after a day how could you stand the smell? Next time soak 4-24 hours then bring them up to a simmer/boil for ~10M then dry let them steam dry.(see the RR let's grow mushroom video for grain prep and the toilet paper test)
They should shake just fine, re watch the grain prep videos. also some gypsum helps
and, take a better picture
Also the foil should be taken off of the lid after the PC cycle. Take a picture of your jar lids for us too, I have a feeling there's something wrong with them.
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Zeros
Hey it's 420



Registered: 11/18/12
Posts: 79
Last seen: 3 years, 3 months
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Re: Rye colonization question [Re: bodhisatta]
#19289524 - 12/17/13 05:01 PM (10 years, 1 month ago) |
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Ok thanks a lot man. Here are my lids.
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Zeros
Hey it's 420



Registered: 11/18/12
Posts: 79
Last seen: 3 years, 3 months
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Re: Rye colonization question [Re: Zeros]
#19289546 - 12/17/13 05:05 PM (10 years, 1 month ago) |
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After sorting through hundreds of pages and teks I just forgot about the lets grow mushrooms series. Thanks for the advice!
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wongyuyee
Stranger
Registered: 12/17/13
Posts: 5
Last seen: 9 years, 10 months
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Re: Rye colonization question [Re: Zeros]
#19289560 - 12/17/13 05:08 PM (10 years, 1 month ago) |
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Just make sure the jars get enough air circulation, i have the same problem last week and i then change the lid with a plastic lid with bigger hole. Then it started growing.
And just a tips : maybe u should try to do liquid culture first then only inject it into jars. With that u will be stun by how fast it will colonize the jars.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Rye colonization question [Re: wongyuyee]
#19289569 - 12/17/13 05:10 PM (10 years, 1 month ago) |
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Quote:
wongyuyee said: Just make sure the jars get enough air circulation, i have the same problem last week and i then change the lid with a plastic lid with bigger hole. Then it started growing.
And just a tips : maybe u should try to do liquid cultureagar first then only injectdrop a wedge into jars. With that u will be stun by how fast it will colonize the jars.
welcome to shroomery bro
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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wongyuyee
Stranger
Registered: 12/17/13
Posts: 5
Last seen: 9 years, 10 months
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Re: Rye colonization question [Re: cronicr]
#19289590 - 12/17/13 05:14 PM (10 years, 1 month ago) |
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hehehe thanx!! actually i decide to join shoomery becoz i wanted to share something that ive tested..
I have spore print that i bought 5 years ago... and on 1st december 2013 i was curious about weather the spore will be dead or not... so i try to grow.. and it turn out very very succesful.. heheh this is what i really wanna share with everyone.
So far the spore that confirm still alive is Mexico, B+, GT, A+.. and the one KIV is china, elephant dung, panama. Still not tested is brazil.. hehehe
Hope this helps alittle
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Rye colonization question [Re: wongyuyee]
#19289608 - 12/17/13 05:18 PM (10 years, 1 month ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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Re: Rye colonization question [Re: cronicr]
#19289619 - 12/17/13 05:20 PM (10 years, 1 month ago) |
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Lmao you have a PC and spores. Get into agar. You will learn how sterile you really are and you will benefit alot from it.
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