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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: Pastywhyte]
#19290043 - 12/17/13 06:43 PM (10 years, 1 month ago) |
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naturally we torch shit also.

the annie difference........
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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: Pastywhyte]
#19290082 - 12/17/13 06:51 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said: I dunno nothing say's sterile tools like a red hot glowing scalpel Then again you don't play with fire or knives eh? Probably for the best I guess 
Myself, I like me some fire. Burning things is fun.
And I used the same scalpel to drill holes in a plastic plant-pot to hold some poles in place for my HBWR to climb up.
The scalpel is mostly plastic though. Will I deform it or something if I make the blade red hot?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19290098 - 12/17/13 06:54 PM (10 years, 1 month ago) |
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Not if the end that the blade is fixed to is metal. You should have it red hot tho, this ensures it stays sterile when bringing it back into the SAB.
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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: Pastywhyte]
#19290158 - 12/17/13 07:13 PM (10 years, 1 month ago) |
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Unfortunately the blade is fixed directly into plastic. Am I screwed?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19290190 - 12/17/13 07:19 PM (10 years, 1 month ago) |
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Give it a try, but I would get a better one cause that thing ain't gonna last and you want something you can eventually change the blade on. A simple metal exacto scalpel works good.
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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: Pastywhyte]
#19290226 - 12/17/13 07:29 PM (10 years, 1 month ago) |
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Damn why didn't I think of that? Exacto sounds good.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19290435 - 12/17/13 08:18 PM (10 years, 1 month ago) |
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Its the little things that make the diffrence
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FrogMachine
Bacon based life form



Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: cronicr]
#19314183 - 12/22/13 11:33 PM (10 years, 1 month ago) |
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Got some kinda growth... Of something! :P Something white and fluffy looking... In spots of varying size.
I honestly thought the thing was dead, but I was wrong. Whatever it is, something's alive in there...

Should I be excited? Is this likely mushroom mycelium? I'm no expert.
Sorry for the crappy picture. With all the condensation it's hard to see what I'm looking at and I clearly don't want to pop the top off just to get a better look.
Oh and I think I ought to mention that I've got such growth in both containers and 2 glass jars. 4 other jars are void of visible life.
Got a better picture now... The glass jars don't have condensation inside so they're easier to see into.
Edited by FrogMachine (12/22/13 11:50 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19314296 - 12/23/13 12:22 AM (10 years, 1 month ago) |
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Nice stuff!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FrogMachine
Bacon based life form



Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: cronicr]
#19389086 - 01/08/14 04:35 PM (10 years, 22 days ago) |
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Okay it's been a while since I started this thread, but I've got a couple pictures of the progress I've made.


The first is my fully colonized small jars with the smaller amount of rice. The second is one of my larger plastic jars with the larger amount of rice, not fully colonized, colonizing with much less vigor than the other two, and with what I suspect is a bacterial contamination.
If you're wondering what happened to the second plastic jar... :P A friend of mine was rather excited about the project so I let him take it and watch it go. I have no idea if it's doing better than the one I've got.
On a side note, I have some new syringes ready... Nice and dark. I have a good feeling about these ones. I also have four spare plastic jars, and I have the 4 dead glass jars to re-use.
The small glass jars have been fully colonized for a few days now. I suppose I should slightly open the lids and expose them to gas exchange. Is there anything else I should do to them?
The suspected contaminated plastic jar I'm just watching progress (however slowly) out of curiosity. The mycelium does seem to be creeping to the bottom, slowly but surely, without the same texture I saw in the little glass jars.
Before I make up new jars, I need to make up a better still-air environment. Happen to be broke right now though so maybe not right away. But I'll get to it at some point.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19389110 - 01/08/14 04:38 PM (10 years, 22 days ago) |
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a cardboard box and saran wrap was my first still air box, and it worked great, looking good to btw
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FrogMachine
Bacon based life form



Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: cronicr]
#19389172 - 01/08/14 04:45 PM (10 years, 22 days ago) |
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I do have saran wrap and cardboard boxes.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19389192 - 01/08/14 04:47 PM (10 years, 22 days ago) |
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Quote:
FrogMachine said: I do have saran wrap and cardboard boxes.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FrogMachine
Bacon based life form



Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: cronicr]
#19445417 - 01/19/14 11:42 PM (10 years, 11 days ago) |
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Update:
I made a cardboard box still air setup and did up the remaining 4 plastic jars. Brown rice made in a rice cooker, put in the jars, and pressure cooked for an hour. This was a week ago. Not seeing anything yet but not worried.
Yesterday I noticed the colonized substrates in my little glass mini-jars with the plastic lids were looking like they were shrinking away from the glass, and I saw no condensation. I figured they could use some of that bottom watering, and so I used a spray bottle to squirt at the sides of the jar and let the water run to the bottom.
So today I took a look and what do I find but what appears to be a little bit of pinning. I'm excited to see little brownish bumps, even if they're on the side of the jar.
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FrogMachine
Bacon based life form



Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19458452 - 01/22/14 04:22 PM (10 years, 8 days ago) |
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A little more progress. :P That first little pin is growing up to be a happy little mushroom. And I have more pins just showing up now on the other mini glass jar's substrate.
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 5 months, 20 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19460249 - 01/22/14 10:58 PM (10 years, 8 days ago) |
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Nice. You didn't case the rice? A little moist verm on top? You'd get a lot more water hence mushroom weight than straight grains without a casing.
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FrogMachine
Bacon based life form



Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: vikingsc]
#19460344 - 01/22/14 11:24 PM (10 years, 8 days ago) |
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Yeah, it's totally uncased, but I'm starting to consider casing for future attempts. I can see all my pins are on the side where it's moist. At the moment though I'm just happy to see something.
I think I'll try casing with potting soil first, since that's what I happen to have handy. Got bags full of it, and I'm broke.
My next goal though is to save some mycelium in a syringe, using popcorn and the inside of a mushroom, probably that one that sprung up first. I'll try to get some spore prints down too. My main concern is to get beyond the first life-cycle and get some continuity going.
Now I've got to do some reading on preparing casing materials...
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 5 months, 20 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19460424 - 01/22/14 11:48 PM (10 years, 8 days ago) |
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Quote:
FrogMachine said: Yeah, it's totally uncased, but I'm starting to consider casing for future attempts. I can see all my pins are on the side where it's moist. At the moment though I'm just happy to see something.
I think I'll try casing with potting soil first, since that's what I happen to have handy. Got bags full of it, and I'm broke.
My next goal though is to save some mycelium in a syringe, using popcorn and the inside of a mushroom, probably that one that sprung up first. I'll try to get some spore prints down too. My main concern is to get beyond the first life-cycle and get some continuity going.
Now I've got to do some reading on preparing casing materials...
http://www.shroomery.org/forums/showflat.php/Number/19219682
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FrogMachine
Bacon based life form



Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: vikingsc]
#19460570 - 01/23/14 12:29 AM (10 years, 8 days ago) |
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Oh yeah I remember that thread. It was a good one. I just can't seem to figure out what casing was used.
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 5 months, 20 days
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19460589 - 01/23/14 12:35 AM (10 years, 8 days ago) |
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For invitro grains straight verm would be fine.
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