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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Second attempt/first post - cubes in brown rice
#19286529 - 12/16/13 11:13 PM (10 years, 1 month ago) |
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This is my first attempt in a long time, so I'm just going to say more or less what I did and hope for general input, ideas, whatnot. I've been lurking for years so I see no point asking questions till I have some big problem I can't search an answer for.
I'm new, so I don't know everything despite how much reading I've done in the past several years. But I'm doing things that may or may not be wise just for personal experience.
Last time I tried, I was living in a desert. I did WBS to coir-coffee cakes with shiitake culture. They were doing really well until I popped them in the FC and dried them right out despite misting and all that jazz.
The basics:
-I'm cultivating cubensis. -I'm using straight brown rice the whole way through. -I'm starting with grain petris in some tiny glass jars I had handy, with screw-top lids. -I've got some of those nice 1L plastic screw-top containers from Violet's write-ups. -I used a rice cooker for the initial cooking before throwing the jars in a pressure cooker. -I also set up a couple of those 1L plastic containers for in-vitro multispore attempts just for the hell of it. -I have a spore print handy. Not going to bother with syringes for now.
The not-quite-as-basics:
I took a tote I had handy and made it into a still-air box. I put a big clear recycle bag on top and taped some nylon gloves into a couple holes I made in the bag. I have alcohol wipes in the box. I cleaned the box. I guess it's become a glove box. I also have a lighter in the box. I also put the spore print in the box.
Once the jars have cooled down enough (I already PCed them, lids slightly loose), I plan to take them out of the PC and put them in the box (and pull the bag's opening back over the top). Then I'll rip open a wipe and sanitize as much of the inside of the box as possible. Then I'll wait a little while longer for the air to settle somewhat.
Then I'll carefully scrape spores into each of the jars/containers and seal them up and wait. I'll probably even just leave it all in the same box and put the box in my constantly heated bedroom.
If all goes well, I hope to see colonization. And then I will share pictures...
I may pick pins off the mini-jars and use them. I don't know if I'll get contams from my own inexperience or from crappy disreputable prints, but if I do, I may have a chance to keep narrowing things down till I have something useable.
But I have only begun so... We'll just have to see how this goes and take it as it comes.
A couple points on my intentions before I take on some suggestions:
-I'm leaning toward tiny-scale, in vitro. -I'm staying clear of cakes for now. I may no longer live in the desert, but I'll probably cakes/blocks again when I get back into edibles. -I'm really liking the idea of just using rice for now. -I'm going to grab a hygrometer soon just to keep an eye on humidity. -I use so little that really, I'd be pretty much satisfied with the products of a few in vitro plastic containers. -I used to want to try a humidifier but I live on the coast now. -I have a big ol' sack of perlite from when I made my own cactus soil... -I will probably try bottom watering, and I may even try doing that with diluted coffee, or at least putting coffee into the process somewhere.... (I heard Nitrogen and Phosphorus are especially useful and both are plentiful in coffee)
I'm still trying to decide on a casing layer. I'll probably try multiple types of casings and no-casing in different containers. I'm not picky so I'm open to using less-than-awesome casings just 'cause I have some things handy. I happen to have potting soil. I have coir too. I don't have verm at the moment.
I still intend to do some more reading in that area...
I'm also trying to decide what if anything I want for a fruiting chamber. If I go invitro, I'm under the impression that I can fruit without using a chamber, but I'm also under the impression that a tub full of moist perlite couldn't hurt.
Any friendly suggestions/comments will be welcome.
Edit:
Here I go trying to add a picture I just took... Just to show what I'm currently up to in this project... Just 'cause...

Apparently I successfully put an image in my post! Yay!
Edited by FrogMachine (12/17/13 11:56 PM)
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PocketRevolution
Stranger
Registered: 12/22/09
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Last seen: 23 days, 18 hours
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19286670 - 12/17/13 12:10 AM (10 years, 1 month ago) |
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Liquid coffee is really only a good idea if you will be keeping it sterile until it is fully colonized by mycelium. I don't know if you've ever left a pot of coffee sitting for a couple days, but the stuff is like superfood for mold. If you water your cakes with it, they will mold, guaranteed.
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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: PocketRevolution]
#19286779 - 12/17/13 12:45 AM (10 years, 1 month ago) |
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Yeah I've heard the same thing. And I've seen moldy coffee too. Yummmm.
An update: The print I ended up with was TINY and difficult to work with... So I fell back on a couple syringes I had lying around.
Said syringes came from same unnamed source and the one I tried (used half of) didn't germinate, back when I was living in the desert. I forgot to mention I tried to grow more than just shiitakes.
At the time I used them on the same batch of WBS that worked just fine with the shiitake mycelium I'd ordered elsewhere. There was no germination whatsoever in any of the jars and I was kind of ticked.
Edit: Oh and the spore syringes are completely clear. When I originally used one of them I suspected they may have been just water.
Edited by FrogMachine (12/17/13 06:28 AM)
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Deadzygote6
Total Badass



Registered: 03/20/09
Posts: 108
Last seen: 9 years, 6 months
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19286818 - 12/17/13 01:08 AM (10 years, 1 month ago) |
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Be Damn sure to let the alcohol dry before lighting your lighter to flame your needle ive made that mistake melted plastic to both my arms and threw my glove box and everything in it across the room
Edited by Deadzygote6 (12/17/13 01:09 AM)
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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: Deadzygote6]
#19286834 - 12/17/13 01:22 AM (10 years, 1 month ago) |
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Yep I thought about that... And that plastic bag got in the way a little too.
But since I decided to fall back on those old potentially faulty syringes, my hopes were kind of low and I decided to forgo little things like flaming the syringe. I have unmodded lids so I was only squirting that mystery fluid into the jar without contact anyways.
If it doesn't turn out, so be it. If I get some mycelium I'll consider myself lucky.
I'll see what happens and get spores elsewhere if nothing good comes of this... And next time of course I'll be more careful with sterile procedure and do something more reliable with the box as well.
Edited by FrogMachine (12/17/13 06:32 AM)
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19287770 - 12/17/13 09:44 AM (10 years, 1 month ago) |
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ill watch this one...... sounds like your off to a good start.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Second attempt/first post - cubes in brown rice [Re: Deadzygote6]
#19287930 - 12/17/13 10:33 AM (10 years, 1 month ago) |
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Quote:
Deadzygote6 said: Be Damn sure to let the alcohol dry before lighting your lighter to flame your needle ive made that mistake melted plastic to both my arms and threw my glove box and everything in it across the room
That's why tools are flamed outside of the box
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: Pastywhyte]
#19288250 - 12/17/13 12:04 PM (10 years, 1 month ago) |
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or , why ya should use bleach wipes instead.......
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Deadzygote6
Total Badass



Registered: 03/20/09
Posts: 108
Last seen: 9 years, 6 months
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Re: Second attempt/first post - cubes in brown rice [Re: anne halonium]
#19288497 - 12/17/13 12:56 PM (10 years, 1 month ago) |
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Yea lesson learned... but looking back I can't help bit laugh at myself
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: Deadzygote6]
#19288501 - 12/17/13 12:58 PM (10 years, 1 month ago) |
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dont feel bad, me and the maid blew up a glove box back in the 80's
point is, bleach wipes work, and are safer
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
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Re: Second attempt/first post - cubes in brown rice [Re: anne halonium]
#19288518 - 12/17/13 01:01 PM (10 years, 1 month ago) |
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hahaha, I wasnt thinking, bit early one morning, sprayed a bunch of lysol in there
wet the bottom with alcohol, than procedded to flame sterilize internally...
Wont do that again.
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The ego is nothing other than the focus of conscious attention.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Second attempt/first post - cubes in brown rice [Re: anne halonium]
#19288751 - 12/17/13 01:54 PM (10 years, 1 month ago) |
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Quote:
anne halonium said: dont feel bad, me and the maid blew up a glove box back in the 80's
point is, bleach wipes work, and are safer
Some people just shouldn't play with fire or explosives
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: Pastywhyte]
#19289000 - 12/17/13 02:54 PM (10 years, 1 month ago) |
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you mean " most"..........
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Second attempt/first post - cubes in brown rice [Re: anne halonium]
#19289027 - 12/17/13 02:59 PM (10 years, 1 month ago) |
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NWA, annie almost blowing herself up, the 80's had it all
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: cronicr]
#19289180 - 12/17/13 03:36 PM (10 years, 1 month ago) |
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thats nothing, we leveled a barn also , and the maid lost her eyebrows and her hair tips in the blast.
sometimes, she has to take one for the team. its what i pay her the big bucks for.
but, thats another story , and another day.
that said, we believe in fire safety now.
sometimes peeps think were " know it alls" we are. me and the maid learned before the age of the internet. we made mistakes, to scale , you cant imagine, leaving wreckage on 3 continents. many on the verge of criminal negligence.
if i really hated you guys, id say nothing and sit back and laugh. thats not the case, im here so ya all can learn , and survive the grow.
dont ignore, laugh and learn.........and live.
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Edited by anne halonium (12/17/13 03:45 PM)
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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: anne halonium]
#19289408 - 12/17/13 04:30 PM (10 years, 1 month ago) |
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Huh, I didn't know about bleach wipes. But I have bleach (which I cleaned the box with) and I have paper towel, so I suppose I could just make some next time around.
I can't help but keep looking at my rice knowing there's no way I'll be able to see anything yet. :P
On a side note, I may have some more syringes coming in soon. :P Can't say much more about that though can I?
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19289674 - 12/17/13 05:31 PM (10 years, 1 month ago) |
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um , bleach wipe primer.
bleach wipes come in a variety of forms. some arent actually bleach. they are common in plastic contianers in every box store some have choro type names.
they are cheap, dont make it complex.
the things work , and avoids all the gas drama of lysol and iso.
use gloves.
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FrogMachine
Bacon based life form


Registered: 12/16/13
Posts: 31
Loc: BC
Last seen: 10 years, 7 days
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Re: Second attempt/first post - cubes in brown rice [Re: anne halonium]
#19289783 - 12/17/13 05:51 PM (10 years, 1 month ago) |
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Ah, I see, thooooose. I'll grab some before my next little misadventure.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: Second attempt/first post - cubes in brown rice [Re: FrogMachine]
#19289797 - 12/17/13 05:54 PM (10 years, 1 month ago) |
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safety , reliability , performance. thats the annie difference.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Second attempt/first post - cubes in brown rice [Re: anne halonium]
#19289998 - 12/17/13 06:35 PM (10 years, 1 month ago) |
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I dunno nothing say's sterile tools like a red hot glowing scalpel Then again you don't play with fire or knives eh? Probably for the best I guess
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