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bluegill
intergalactic toejam



Registered: 11/05/13
Posts: 489
Last seen: 2 years, 11 months
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First signs of colonization - 4 days
#19283238 - 12/16/13 10:49 AM (10 years, 1 month ago) |
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Day 1 - Made (5) BRF cakes and put them in 1/2pint jars. Sterilized for 45 minutes; didn't have time to sterilize for longer. Instant regret!
Day 2 - Let them sit for a day. Continual regret about sterilization.
Day 3 - Decided to re-sterilize them, this time for 1.5 hrs. I steam sterilized them in a large stock pot and let them cool overnight.
Day 4 - I inoculated the jars the next morning, using .5mL multispore solution on two points per jar (opposite sides of each other). I flame sterilized the needle with a butane lighter (I know). I stored the jars in a white garbage bag in a closed cabinet above my refrigerator, a helpful suggestion I found by reading forum posts here. I also threw in an electronic thermometer I had laying around. Temps have been between 81-86F, mostly at a steady 83F.
Day 5 - nada Day 6 - nada Day 7 - I think I started to observe small growth in a couple jars. Day 8 - I checked the next evening, and I was right. The growths observed had spawned over a larger surface area. I'm not completely certain whether they are a contaminant or not because I'm not seeing any ropey mycelium, yet. Day 9 - All jars are showing signs of colonization at the inoculation points. The jars showing earlier growth have several spawning points spread around the inoculation points. Some jars appear to be showing uneven growth patterns, although it's still too early to tell. Since all the growths look similar, I'm more confident that they are mycelium of the target species.
Questions/concerns I have:
CO2/moisture buildup in jars - The jars seem a little dry to me, but obviously there must be moisture content, otherwise they wouldn't grow. From what I've read, the jars are probably on the dryer side because the growths look more like clouds/cobwebs than ropey mycelium. I have the lids on tight, inoculation points uncovered, and the jars are stored lid-up in the cupboard. Should I flip them around to promote gas exchange? Should I loosen up the lids? Is there any way to hydrate them at this point without a syringe? I don't have an empty one on-hand.
Fruiting chamber prep - the container I want to use has a large surface area, but after seeing pictures of huge fruits, I am concerned it is not tall enough. I'd say it's about 11" tall. Is it safe to assume the larger fruits will span out horizontally, or should I just get another container?
Dunking/rolling when birthing cakes - Is this a necessary step to ensure the highest yield for the first flush? How long do the cakes really need to be submerged in the refrigerator? Would 6 hours be enough?
Thanks for the help!
-------------------- "Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: First signs of colonization - 4 days [Re: bluegill]
#19283289 - 12/16/13 11:02 AM (10 years, 1 month ago) |
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Do not flip them. That is the last thing you want to do. I believe but not positive that rhizo vs fuzzy growth is genetic not usually due to conditions. In any case don't worry about that. Your temps are fine but don't need to be that hot. Around 78 is ideal.
Don't worry about c02 build up I don't understand this concern. 11" sounds a little small too me but it could work out. You don't want to skinch on the perlite though. I'd get a bigger tub personally.
Yes dunk and roll. Just follow the teks man.
Good luck.
edit: The re sterilization was a mistake. They can sit for weeks without being nocced up and be just fine. That could have made your cakes too dry but I guess you will just have to wait and see. Start another batch if you are that concerned.
Edited by d0urd3n (12/16/13 11:04 AM)
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Re: First signs of colonization - 4 days [Re: bluegill]
#19283291 - 12/16/13 11:02 AM (10 years, 1 month ago) |
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I think your temps are too high. I believe 80 is as high as you want to go, any higher, and contams will be more likely.
If you followed the tek for brf, then don't worry about the moisture, it will be ok. The gas will exchange through he dry verm layer, do not flip them over. Just leave them.
There's a common consensus on this forum for noobs (like myself) to not change anything in any of the teks, and for good reason. The questions you are asking are in complete contradiction to this, but i'll answer them for you anyway, because i was tempted to ask them myself, but kept on reading.
Quote:
bluegill said:
Dunking/rolling when birthing cakes - Is this a necessary step to ensure the highest yield for the first flush? How long do the cakes really need to be submerged in the refrigerator? Would 6 hours be enough?
Thanks for the help!
After you birth them, then yes, dunk them for 24 hours, not 6, 8, 9, 14....you want 24. If you can't dunk them that long for whatever reason, then as close to 24 as you can. The roll helps the cake retain moisture right at the cakes surface creating a micro-climate. It will also catch the mist and prevent little puddles forming on your cakes, which is something you don't want. Evaporation plays a big part in pinning, and the verm has more surface area than the cake, so overall, more moisture will evaporate from the cake as a whole this way....then steps in the misting/fanning schedule.
Keep reading, and use the "search" button at the top. You will answer alot of your own questions that way, and learn a fuck-ton in the process.
Good luck man
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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bluegill
intergalactic toejam



Registered: 11/05/13
Posts: 489
Last seen: 2 years, 11 months
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Re: First signs of colonization - 4 days [Re: 1down5up]
#19283606 - 12/16/13 12:18 PM (10 years, 1 month ago) |
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Thanks for the tips.
I did relocate the jars to another cupboard with a temp of about 76.2F. A closer look at the mycelium in daylight - they actually look like mycelium growths.
-------------------- "Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: First signs of colonization - 4 days [Re: bluegill]
#19283610 - 12/16/13 12:19 PM (10 years, 1 month ago) |
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Quote:
bluegill said: Thanks for the tips.
I did relocate the jars to another cupboard with a temp of about 76.2F. A closer look at the mycelium in daylight - they actually look like mycelium growths.
No problem. Great. Pics would be able to answer that question.
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bluegill
intergalactic toejam



Registered: 11/05/13
Posts: 489
Last seen: 2 years, 11 months
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Re: First signs of colonization - 4 days [Re: d0urd3n]
#19302743 - 12/20/13 12:37 PM (10 years, 1 month ago) |
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Day 13 - Lookin' good so far?
Colonization is taking place evenly for the most part. I am a little worried about the middle jar and right jar - they have uneven growth, namely the far right jar. The side you see in the pic is already colonizing the bottom of the jar. The other side of the jar looks something like what you see in the middle jar. I'm worried about unevenness because I (doh!) only used 2 inoculation points (0.5mL each) instead of four.
I also inoculated another set of jars, this time with more H2O in the substrate, and inoculated at 4 different points... and I placed it in a more temp-controlled environment for incubation.
-------------------- "Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: First signs of colonization - 4 days [Re: bluegill]
#19302748 - 12/20/13 12:39 PM (10 years, 1 month ago) |
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don't worry about the uneven growth those look great, as for your other set with more water it may have issues after awhile as gravity will draw that water to the bottom and compact your sub
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bluegill
intergalactic toejam



Registered: 11/05/13
Posts: 489
Last seen: 2 years, 11 months
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Re: First signs of colonization - 4 days [Re: cronicr]
#19302845 - 12/20/13 01:06 PM (10 years, 1 month ago) |
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Quote:
cronicr said: don't worry about the uneven growth those look great, as for your other set with more water it may have issues after awhile as gravity will draw that water to the bottom and compact your sub
Thanks - I will keep this in mind. I haven't observed any noticeable difference with the new jars. I added maybe 1/8 of the cup to the ratio recommended by PF tek just to see if it works better. The jars haven't shown signs of colonization, yet.
-------------------- "Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."
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bluegill
intergalactic toejam



Registered: 11/05/13
Posts: 489
Last seen: 2 years, 11 months
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Re: First signs of colonization - 4 days [Re: bluegill]
#19355764 - 01/01/14 06:20 PM (10 years, 29 days ago) |
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Happy New Year to all, and coincidentally, I checked on my first batch tonight and the jar with the most rapid growth is starting to pin. There are 2 primordia forming toward the bottom of the jar. I guess that means it's fully colonized, huh? I'm thinking I should let it sit for one or two more days before I birth. What do you think? Pics will be posted tomorrow.
-------------------- "Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."
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Paulsage
Shaman of shit storms



Registered: 09/01/12
Posts: 109
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Re: First signs of colonization - 4 days [Re: bluegill]
#19355951 - 01/01/14 07:18 PM (10 years, 29 days ago) |
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looks good...
-------------------- "The brain is a reducing valve that restricts consciousness" - A Huxley "Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson… "Whatever you study you also change" - Heisenberg Uncertainty principle
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bluegill
intergalactic toejam



Registered: 11/05/13
Posts: 489
Last seen: 2 years, 11 months
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Re: First signs of colonization - 4 days [Re: Paulsage]
#19393629 - 01/09/14 12:54 PM (10 years, 21 days ago) |
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The cake in the center was the rapid growth BRF jar. It was the first one I birthed and it had primordia forming in vitro before birth. It's been about a week since I placed it in the FC. Growth seems to be much better than I anticipated because it's only getting partial exposure to light and misting/fanning twice per day.
How much longer do you reckon it will be before they are ready to harvest? I'm hoping tomorrow they are ready! I was patient throughout the whole process, but now it's wearing thin...
-------------------- "Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."
Edited by bluegill (01/09/14 12:55 PM)
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
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Re: First signs of colonization - 4 days [Re: bluegill]
#19393635 - 01/09/14 12:55 PM (10 years, 21 days ago) |
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tommorow you will be harvesting a few of those fruits
great lookin cake also
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Danny Daniels82
MR.


Registered: 09/21/19
Posts: 53
Loc: New York
Last seen: 1 year, 11 months
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Re: First signs of colonization - 4 days [Re: bluegill]
#26247773 - 10/12/19 03:45 PM (4 years, 3 months ago) |
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I'm so jealous . My cakes haven't shown any colonization. I'm waiting like
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iux
Tubaeformis boi


Registered: 03/20/18
Posts: 288
Loc: Norway
Last seen: 5 months, 24 days
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Hey, just a heads up, this post is 5+ years old
-------------------- Remember to be respectful to each other, be kind, and enjoy life in a responsible way
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