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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Dry Fruit Clone
#19281521 - 12/15/13 09:59 PM (10 years, 2 months ago) |
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About 2 years ago, I had some fruits with genetics I really liked.
Unfortunately, I did not know how to use agar and was unable to clone a fruit or preserve a culture and the genetics were lost.
Recently, a friend of mine told me he still had some of those dry fruits. They were well preserved in a mason jar.
I very carefully cut off a couple of pieces of stems and am going to attempt to clone them.

The plan:
In a SAB, I'll try to break open the pieces with my fingers and expose the cleanest part I can. Then, use a flame sterilized scalpel to scrape or cut out a small piece from the middle and put on agar.
I have 6 fresh agar plates cooling right now.
Any tips would be appreciated.
Wish me luck.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Might want to use an antibiotic in your agar. I'm gonna do up so antibiotic plates myself for some wild spore prints I got. Maybe tomorrow.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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I don't have any antibiotics. 
But, If this don't work, I have more dry fruits to work with.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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On your next trip to the pet store, pick up some tetracycline from the aquarium section. Little bit goes a long way. Blue was doing some tests with it in his PDYA agar thread and that's what I got for my antibiotic agar as well.
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silverstem
Caps & Stems



Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 6 years, 6 months
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dont buy premade antibiotic agar... that stuff goes for like 60 a bottle.
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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,403
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When I clicked this thread my first reaction was going to be 'you won't get anything because the cells are dry and 'dead', you are not jesus christ and cannot resurrect a dead and dry life form."
Am I wrong here? Is it really as simple as wiping dry mushroom tissue onto agar? It will seriously bring it back to life?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Word. I'll check that out tomorrow. Thx.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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SuperFly
Still in the Space Race



Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 2 months, 17 days
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It was my understanding that you had to rehydrate them with sterile water. Fungi are fairly resilient little fuckers and I would bet this will work.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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It's well known that dry mycelium can be revived. The trick here is to get ahead of contaminants. The odds are against me though. But what have I got to lose?
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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