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InvisibleSpitballJedi
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Dry Fruit Clone
    #19281521 - 12/15/13 09:59 PM (10 years, 2 months ago)

About 2 years ago, I had some fruits with genetics I really liked.

Unfortunately, I did not know how to use agar and was unable to clone a fruit or preserve a culture and the genetics were lost.

Recently, a friend of mine told me he still had some of those dry fruits. They were well preserved in a mason jar.

I very carefully cut off a couple of pieces of stems and am going to attempt to clone them.



The plan:

In a SAB, I'll try to break open the pieces with my fingers and expose the cleanest part I can. Then, use a flame sterilized scalpel to scrape or cut out a small piece from the middle and put on agar.

I have 6 fresh agar plates cooling right now.

Any tips would be appreciated.

Wish me luck.


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InvisiblePastywhyteMDiscord
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Re: Dry Fruit Clone [Re: SpitballJedi]
    #19281535 - 12/15/13 10:02 PM (10 years, 2 months ago)

Might want to use an antibiotic in your agar. I'm gonna do up so antibiotic plates myself for some wild spore prints I got. Maybe tomorrow.


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InvisibleSpitballJedi
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Re: Dry Fruit Clone [Re: Pastywhyte]
    #19281583 - 12/15/13 10:13 PM (10 years, 2 months ago)

I don't have any antibiotics. :shrug:

But, If this don't work, I have more dry fruits to work with.


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InvisiblePastywhyteMDiscord
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Re: Dry Fruit Clone [Re: SpitballJedi]
    #19281596 - 12/15/13 10:15 PM (10 years, 2 months ago)

On your next trip to the pet store, pick up some tetracycline from the aquarium section. Little bit goes a long way. Blue was doing some tests with it in his PDYA agar thread and that's what I got for my antibiotic agar as well.


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Offlinesilverstem
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Re: Dry Fruit Clone [Re: Pastywhyte]
    #19281611 - 12/15/13 10:21 PM (10 years, 2 months ago)

:whathesaid: dont buy premade antibiotic agar... that stuff goes for like 60 a bottle.


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Invisibleelasticaltiger
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Re: Dry Fruit Clone [Re: silverstem]
    #19281623 - 12/15/13 10:27 PM (10 years, 2 months ago)

When I clicked this thread my first reaction was going to be 'you won't get anything because the cells are dry and 'dead', you are not jesus christ and cannot resurrect a dead and dry life form."

Am I wrong here? Is it really as simple as wiping dry mushroom tissue onto agar?  It will seriously bring it back to life?


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InvisibleSpitballJedi
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Re: Dry Fruit Clone [Re: silverstem]
    #19281624 - 12/15/13 10:27 PM (10 years, 2 months ago)

Word. I'll check that out tomorrow. Thx.


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InvisibleSuperFly
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Re: Dry Fruit Clone [Re: SpitballJedi]
    #19281663 - 12/15/13 10:40 PM (10 years, 2 months ago)



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Re: Dry Fruit Clone [Re: SpitballJedi]
    #19281664 - 12/15/13 10:40 PM (10 years, 2 months ago)

It was my understanding that you had to rehydrate them with sterile water. Fungi are fairly resilient little fuckers and I would bet this will work.


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InvisibleSpitballJedi
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Re: Dry Fruit Clone [Re: elasticaltiger]
    #19281703 - 12/15/13 10:56 PM (10 years, 2 months ago)

It's well known that dry mycelium can be revived. The trick here is to get ahead of contaminants. The odds are against me though. But what have I got to lose?


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