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OfflineStromriderM
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What a waste of time and resources
    #19278043 - 12/15/13 07:28 AM (10 years, 2 months ago)

I took one my of my spore prints a while back when I first got into agar and proceeded to isolate a single strain on agar. After only 3 transfers I had an isolated culture and made a grain master. I g2g that to 7 more jars and made a monotub with 6 of them and saved the one as a new master.

Well the tub colonized at an average rate and I put it into fruiting conditions. After about 2 weeks in fruiting I started seeing a really nice pin set. I was happy with its progress but then all the caps opened with all the mushrooms only about an inch tall.

I didn't even harvest the Damn things or take any pics. I just chucked it in the woods mushrooms and all. It's pretty aggravating. I guess the moral of the story is when isolating from spores you have just a good of a chance of getting a shit performing culture as you do a good one. I guess I kind of already knew that but wanted to see for myself. Only ms and cloning for me from here on out. Guess I got to learn to make slants so I can save any really awesome cultures

Guess it was a good learning experience :shrug:


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OfflineRogerRabbitM
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Re: What a waste of time and resources [Re: Stromrider]
    #19278057 - 12/15/13 07:33 AM (10 years, 2 months ago)

Quote:

Stromrider said:

Well the tub colonized at an average rate and I put it into fruiting conditions. After about 2 weeks in fruiting I started seeing a really nice pin set. I was happy with its progress but then all the caps opened with all the mushrooms only about an inch tall.





This was a substrate management problem. 

Whenever you have a massive pinset, you have to mist and/or water the substrate enough to support them.  If not, they open the caps while very small.  It sounds like you trashed an awesome strain.  Furthermore, small mushrooms are more potent by weight than large ones so they're what you want for eating.

Next time, don't isolate one strain.  Isolate a dozen or more and test each one.  Test with brf cakes so it's faster and you don't waste a lot of time or money on the bad strains.
RR


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OfflineJMcDoogle
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Re: What a waste of time and resources [Re: RogerRabbit]
    #19278070 - 12/15/13 07:36 AM (10 years, 2 months ago)

Quote:

RogerRabbit said:
Quote:

Stromrider said:

Well the tub colonized at an average rate and I put it into fruiting conditions. After about 2 weeks in fruiting I started seeing a really nice pin set. I was happy with its progress but then all the caps opened with all the mushrooms only about an inch tall.





This was a substrate management problem. 

Whenever you have a massive pinset, you have to mist and/or water the substrate enough to support them.  If not, they open the caps while very small.  It sounds like you trashed an awesome strain.  Furthermore, small mushrooms are more potent by weight than large ones so they're what you want for eating.

Next time, don't isolate one strain.  Isolate a dozen or more and test each one.  Test with brf cakes so it's faster and you don't waste a lot of time or money on the bad strains.
RR




I like this guy!

* watches Strom scramble to the woods to retrieve his nearly frozen mono *


--------------------
The ego is nothing other than the focus of conscious attention.


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OfflineStromriderM
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Re: What a waste of time and resources [Re: RogerRabbit]
    #19278085 - 12/15/13 07:43 AM (10 years, 2 months ago)

Well I still have the culture as a fully colonized master in the fridge but I'm pretty sure it's shit judging by the little funcky looking mushrooms


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InvisibleViolet
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Re: What a waste of time and resources [Re: Stromrider]
    #19278141 - 12/15/13 08:07 AM (10 years, 2 months ago)

Oh that definitely happens.  Yes it's always possible that they grew differently due to conditions than they would want to otherwise, but if you know what you're doing and do it well but they Still form that way then I would place my bets that you're right.

I've had some successes with blind isolation, but they're almost never the best successes.

Hence the grain "petri"
I'm beginning preliminary culture tests on 20+ clone isolates taken in such a manner, and All of them are not only fruiters of full and healthy fruits as expected but also with some pretty decent yield indications so far to boot.


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OfflineStromriderM
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Re: What a waste of time and resources [Re: Violet]
    #19278158 - 12/15/13 08:16 AM (10 years, 2 months ago)

Yeah I  grew under identical conditions of other successful tubs so I figured it had to be the culture itself


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Invisiblebootster
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Registered: 02/22/11
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Re: What a waste of time and resources [Re: Stromrider]
    #19278230 - 12/15/13 08:44 AM (10 years, 2 months ago)

I just got done with the same results (Chitwan). I used a 1 1/2" thick substrate ant they all were about 2" tall. I tested some and there's no way that's getting chucked. A little yield is better than no yield and it turns out, I printed some caps because they were awesome yet tiny (isolate).

I flipped the sub over (no liner) and there are probably 100 of them ready to stand up to the light or get harvested and dunked. Blue is what they do. I was ready to give up on them but waited it out for 8 weeks and it started pinning. At 9 weeks they were harvested and the tub flipped over last night (wasn't easy).

Strom, go get that tub!


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OfflineStromriderM
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Re: What a waste of time and resources [Re: bootster]
    #19278435 - 12/15/13 09:50 AM (10 years, 2 months ago)

I'm just not willing to put a bunch of time and effort into a culture. If it doesn't fruit well I say start a new one. I wanna see a bunch of big pretty fruits like the tub in my signature. If it can't fruit like that than into the woods you go! :lol:


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Invisiblebootster
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Re: What a waste of time and resources [Re: bootster]
    #19278493 - 12/15/13 10:04 AM (10 years, 2 months ago)

I must have bad luck with boomers. Every time I get a flush of them, they aren't as potent as mid to small sized ones. :shrug:


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