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Offlineislander20
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Registered: 01/16/08
Posts: 556
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Spawn Bag Transfers?
    #19277679 - 12/15/13 04:04 AM (10 years, 1 month ago)

Hello,

The past few days I have been moving my Pink Oyster spawn into some enriched sawdust bags. I am having problems with my sterile technique, just can't seem to find a good way to move the spawn out of one spawn bag and into another. All of the videos and tek's I find assume your grain spawn is in jars. I am using the big 5 pound bags in a glovebox.

So far i have been using a spoon to scoop out spawn and transfer it over to my sawdust blocks. I sterilized the spoon in my pressure cooker wrapped in tinfoil. Then I opened it in the glovebox and kept it in a jar of bleach water. The whole process did not go very well, im sure most of my bags are contaminated.

Any tips or tricks for doing spawn bag transfers?

Thanks!


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OfflineSkysTheLimit
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Registered: 07/28/13
Posts: 168
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Re: Spawn Bag Transfers? [Re: islander20]
    #19277785 - 12/15/13 05:02 AM (10 years, 1 month ago)

Did you spray down your glovebox with lysol or bleach and wait an hour for it to settle?

EDIT: And wipe down your hands with some sort of liquid to prevent spores/dust flying/bouncing off?


(Just some common mistakes I've found out ive made too)


Edited by SkysTheLimit (12/15/13 05:03 AM)


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Offlineislander20
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Registered: 01/16/08
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Re: Spawn Bag Transfers? [Re: SkysTheLimit]
    #19278479 - 12/15/13 10:01 AM (10 years, 1 month ago)

Yes I sprayed the room with lysol, and wet the inside of the box down with bleach water. Ai use my box upside down on a wet towel. I cant wait an hour for things to settle, I can only fit two of the 5 pound bags in my box at a time. So for me to do for would take over 4 hours! ( Thats one sawdust block and the spawn bag in together).

My main problem was the scooping out of the spawn bag and into the sawdust bag. Just seems like such an easy path for contamination. Im thinking about cutting a slot on either side of the glove box to put the top of the spawn bag through. Would give me a lot more room, and would be easier to get things done. Trying to think of some sort of rubber seal that would some what seal the slot around the spawn bag.


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OfflineForresterM
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Re: Spawn Bag Transfers? [Re: islander20]
    #19278504 - 12/15/13 10:06 AM (10 years, 1 month ago)

Quote:

islander20 said:
I cant wait an hour for things to settle, I can only fit two of the 5 pound bags in my box at a time. So for me to do for would take over 4 hours! ( Thats one sawdust block and the spawn bag in together).




If you don't wait for the air to get "still" and spores to settle, you're kind of defeating the point of a still air box, aren't you?

Just saying, I had contamination problems until I started letting it sit longer before working.  There's really no way around that, short of getting a flow hood.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinedeadmandave
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Re: Spawn Bag Transfers? [Re: Forrester]
    #19278587 - 12/15/13 10:20 AM (10 years, 1 month ago)

maybe to make pouring of the bag easier you could break up the spawn first, then inside the s.a.b. cut most of the excess bag off. then it will be a lot easier to manipulate and could be able to pour instead of using a spoon.

though it will still be a problem when moving new bags in and out of the box because of dust and spores being swept into the grain spawn. :shrug:


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OfflineSkysTheLimit
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Registered: 07/28/13
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Re: Spawn Bag Transfers? [Re: Forrester]
    #19280250 - 12/15/13 05:01 PM (10 years, 1 month ago)

Quote:

Forrester said:
If you don't wait for the air to get "still" and spores to settle, you're kind of defeating the point of a still air box, aren't you?

Just saying, I had contamination problems until I started letting it sit longer before working.  There's really no way around that, short of getting a flow hood.




^I had the same problem as Forrester
If your not spraying the glovebox with your work inside and waiting an hour or so, then you might as well do the transfer on your kitchen  table.

And if time is a issue, sounds like this cultivation project is serious and you'll need to invest in a flowhood.

Maybe you could get a larger SAB?


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Offlinemister brown 420
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Re: Spawn Bag Transfers? [Re: SkysTheLimit]
    #19282419 - 12/16/13 04:28 AM (10 years, 1 month ago)

Multiple still air boxes?
stacked on top od one another or side by side!


Edited by mister brown 420 (12/16/13 04:38 AM)


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OfflineSkysTheLimit
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Re: Spawn Bag Transfers? [Re: mister brown 420]
    #19282473 - 12/16/13 05:08 AM (10 years, 1 month ago)

That wouldn't be a bad idea, but i was thinking more along the lines of a SAB with some space just for the spawn bags, as long as its been sprayed down properly it should be fine. But damn, you should really just buy a flowhood.


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Offlinemister brown 420
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Re: Spawn Bag Transfers? [Re: SkysTheLimit]
    #19282503 - 12/16/13 05:26 AM (10 years, 1 month ago)

:holyshitbubbles:

Ask Santa nicely!


--------------------
Estoy perdido y no me importa'
deje que se vaya y que sea.
hay amor.
Last on
7/7/2014
See you space cowboy!
:asianofapproval:


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Offlinet3chnobily
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Re: Spawn Bag Transfers? [Re: mister brown 420]
    #19282644 - 12/16/13 07:07 AM (10 years, 1 month ago)

Get a flow hood.  Bag to bag is not really viable in a SAB.  If you must use a SAB then stick with jars to bag transfers.  With a 96 quart SAB I could inoculate 5 bags at a time.  Yes you must wait an hour in between cycles.  Even then success is not guaranteed.  Working a box that size requires long arms and feels like you are being subjected to medieval torture.

If you need enough substrate that you can't keep up with production with a few dozen jars then you need a hood.


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Offlineislander20
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Re: Spawn Bag Transfers? [Re: t3chnobily]
    #19282660 - 12/16/13 07:17 AM (10 years, 1 month ago)

Damn im coming to realize that...just finishing up my greenhouse, fuck this hobby gets expensive! Lots of reading to do about building a flow hood now. For the mean time I am thinking about trying to use GLC for increasing spawn and using pastuerized sawdust for my final substrate.

I had some leftover rye grain ( boiled and dryed) that I used to make "spawn pockets" in some of my sawdust logs yesterday. Might be a good technique for me to avoid having to do transfers, for the meantime anyways.

Thanks for all the help and suggestions.


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OfflineForresterM
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Re: Spawn Bag Transfers? [Re: islander20]
    #19282805 - 12/16/13 08:31 AM (10 years, 1 month ago)

Quote:

islander20 said:
I had some leftover rye grain ( boiled and dryed) that I used to make "spawn pockets" in some of my sawdust logs yesterday. Might be a good technique for me to avoid having to do transfers, for the meantime anyways.




I've just started experimenting with this as well, just putting a small pile of rye on top of my supplemented sawdust in filter patch bags, and innoculating with a syringe.  Seems to be working so far!


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinet3chnobily
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Re: Spawn Bag Transfers? [Re: Forrester]
    #19283059 - 12/16/13 10:04 AM (10 years, 1 month ago)

somebody sells kits with rye pockets using unicorn injection port bags.  Syringe to fruit in the same bag.  No PC, no glove box, no transfers, maybe no FC.  Looks like a great options for beginners.  Those bags are like a buck a piece though in bulk.


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OfflineSkysTheLimit
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Re: Spawn Bag Transfers? [Re: islander20]
    #19284962 - 12/16/13 04:52 PM (10 years, 1 month ago)

OH YEAH, OP, you can just inoculate from liquid spawn with a syringe inside a SAB!
That way you can put heaps of bags in the sab at one time and with just 1 or more syringes, inoculate them all up without using much space in the SAB.
Or if you use pillow stuffing / poly fill to close off the bag at the top, the syringe can stick right through without any cleaning or possibly even an SAB.

The guy above me gave me that epiphany :grin:


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