Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Kratom Capsules for Sale   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineNorCalCaps
Stranger

Registered: 11/20/07
Posts: 15
Last seen: 9 years, 4 months
Slow Colonization... What To Do?
    #19270589 - 12/13/13 12:38 PM (10 years, 2 months ago)

This is my first run at studying the growth of mycelium and the fruiting process. I have been lurking in the shadows on these forms for several years now.

What I have:

I purchased six BRF bags from O.G. and three fast growing strains from R.S. My clean room was freshly painted before this project and literally scrubbed down to every nook and cranny. I've been careful with my clothing, gloves, masks ...etc. to keep the environment clean during inoculation and ever since.

Inoculation took place on November 1st, where I injected 5cc's per bag. It has now been six weeks. Two of the specimens are looking good, but still not fully colonized. The other four all have mycelium growth and no dark spots or other signs of contamination. They look healthy - bright white - but are just colonizing really really slow. I might even say they've stopped growing - but what is there looks health (sorry I don't have pictures right now to post - I can take some and post them later if need be).

They are stored in an out-building which tends to get cold this time of year, so I have the bags inside a double clear container setup with water and a fish tank heater in the outer tub - where the temp is maintained around 77ºF (+/-). They do get some indirect sunlight through a nearby window and recently I put in a CFL rated at 6500k which runs 8 hours a day, during sunlight hours. I started the bags off in complete darkness (based on something I had read) - and they did well (initially), but as I read more and saw that colonization had almost stopped I started reading and found there are 20 ways to skin a cat and even more ways to colonize. The bags are still rolled up at this point - and I have read some threads that say to unravel them to allow for gas exchange... yes? no? I really not sure what to do next...

Should I dunk tek 'em at this point? Or is that only good after the first fruiting (as I've read)?

Any help would be appreciated.

Thanks!


Edited by NorCalCaps (12/13/13 12:39 PM)


Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: Slow Colonization... What To Do? [Re: NorCalCaps]
    #19270678 - 12/13/13 01:05 PM (10 years, 2 months ago)

Pics would probably help a lot. If you're sure they've stopped growing then I'd probably shake them up as well as try to inflate the bags through the GE port. If they're still growing then you're just being impatient. 6 weeks is a long time though, but it is MS.


Extras: Filter Print Post Top
OfflineNorCalCaps
Stranger

Registered: 11/20/07
Posts: 15
Last seen: 9 years, 4 months
Re: Slow Colonization... What To Do? [Re: krypto2000]
    #19270783 - 12/13/13 01:34 PM (10 years, 2 months ago)

Thanks for the info. I shook the bags about 2 weeks ago, but the substraight is moist and does not break apart at this point, so they did really shake up well at all - as in - nothing really moved around. So I broke the cakes in a few places to see if growth would spread. No real luck thus far.

What about light. I keep reading to keep them in the dark - then I see that indirect sunlight is good. There are so many conflicting opinions - I don't know which way to go. I am glad they're healthy still, but don't know how to spur further colonization at this point.


Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: Slow Colonization... What To Do? [Re: NorCalCaps]
    #19270948 - 12/13/13 02:09 PM (10 years, 2 months ago)

Light doesn't really matter during colonization either way, won't help or hurt as I understand it. What do you mean the whole thing was a cake? That almost makes me imagine it's way oversaturated with water and turned into a giant block. Are the grains burst? Pics would really help, you think you can get any later? Oh wait, this is brf, sorry :facepalm:... I haven't heard of brf in bags, how are you supposed to fruit them, in vitro?


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
Trusted Cultivator
Re: Slow Colonization... What To Do? [Re: krypto2000]
    #19271112 - 12/13/13 02:43 PM (10 years, 2 months ago)

light will help, it's been proven benificial at all stages of growth, ambient lighting during colonizing,intense/direct during fruiting, they have a circadian rhythm like you and i


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineNorCalCaps
Stranger

Registered: 11/20/07
Posts: 15
Last seen: 9 years, 4 months
Re: Slow Colonization... What To Do? [Re: krypto2000]
    #19271140 - 12/13/13 02:52 PM (10 years, 2 months ago)

I'll have my camera back tomorrow and will shoot them then. I just went out to shake then again and they had really hardened up into bricks, so I made sure they broke up and shook well this time. I noticed in two bags some light brown coloring. Not sure if they got contaminated or are pinning, as they have been near light recently.

I will probably grow them in vitro. I have the light off now.


Extras: Filter Print Post Top
OfflineTheWiz
Happy Little Shroom
 User Gallery


Registered: 11/21/11
Posts: 191
Loc: Southern IL
Last seen: 3 years, 4 months
Re: Slow Colonization... What To Do? [Re: cronicr]
    #19271150 - 12/13/13 02:54 PM (10 years, 2 months ago)

Unravel them to allow for gas exchange.  You should have done that on day 1.

DO NOT OPEN ANYTHING THAT IS NOT FULLY COLONIZED UNLESS YOU INTEND TO THROW IT OUT.  You will end up with contaminant mold, your environment will get fucked up for future grows, and you will likely not get any mushrooms anyway.


--------------------
I'd hit it.


Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: Slow Colonization... What To Do? [Re: cronicr]
    #19271286 - 12/13/13 03:27 PM (10 years, 2 months ago)

Quote:

cronicr said:
light will help, it's been proven benificial at all stages of growth, ambient lighting during colonizing,intense/direct during fruiting, they have a circadian rhythm like you and i




Do you have a citation for it's benefits during colonization? What exactly does it do, and how does it help? Mushrooms don't naturally get much, if any, light during colonization so I'm skeptical.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
Trusted Cultivator
Re: Slow Colonization... What To Do? [Re: krypto2000]
    #19271364 - 12/13/13 03:49 PM (10 years, 2 months ago)

there's actually alot of conflicting info even from rr himself on that

You don't need your stuff to be in the dark at any time, period.  Mycelium will colonize faster when exposed to low level ambient room light on a day/night schedule.  Turn up the intensity at fruiting time, but don't colonize in the dark.

In nature, spores are released into the wind to settle where they might.  You can't assume that all happens in the dark.
RR
http://www.shroomery.org/forums/showflat.php/Number/16367769#16367769
then he says this here

Normal room lighting has no effect on colonizing mycelium, either good or bad. This is one of the old myths perpetuated by stamets 'The Mushroom Cultivator', that was incorrect 20 years ago when it was written. He corrected his own mistake in GGMM, but fewer people have read that one because it centers more on edibles, than on psilocybe mushrooms. My own research says light doesn't make one whit of difference to colonizing mycelium in jars.

The time to protect from light is during spawn run of bulk substrates and casing layers. During the initial colonization of grains or brf cakes, it doesn't matter.
RR
http://www.shroomery.org/forums/showflat.php/Number/7004679#7004679

that was 6 years ago, this is from a year ago

Ambient room light is very beneficial for colonizing mycelium, but avoid direct sunlight until time for fruiting.

Shiitake substrates are ready for fruiting up to a few weeks earlier if exposed to light from day one.  P cubensis will colonize a bit faster and pinning happens sooner if low-level light is used during the colonization phase.
RR
http://www.shroomery.org/forums/showflat.php/Number/17181811#17181811

and then
Normal ambient light is irrelevant early in colonization, but there's a LOT of evidence pointing to light exposure during the last week of colonization to speed up the entire fruiting cycle.  Shiitake growers learned this a few decades ago.  By exposing to light during colonization, they get pins sooner and overall flushes are improved.  Mycelium has circadian rhythms, just like humans.  Light and a day/night temperature fluctuation helps to establish this rhythm.
RR
http://www.shroomery.org/forums/showflat.php/Number/10708570#10708570
so really as far as i am concerned your statement..

Quote:

krypto2000 said:
Light doesn't really matter during colonization either way, won't help or hurt as I understand it.


...isn't really far off:shrug:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offline1down5up
Social Ninja
Male User Gallery

Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
Re: Slow Colonization... What To Do? [Re: cronicr]
    #19271529 - 12/13/13 04:29 PM (10 years, 2 months ago)

It takes a child between 9-12 months on average to learn to walk, and about 4 weeks after learning to walk, they can run.  Patience bro, let them colonize, and when they do, they will fruit pretty fast in comparison.  :nyan:

^^^I don't know what flying toaster cat is, but it's funny.  Good luck my friend!


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: Slow Colonization... What To Do? [Re: cronicr]
    #19271540 - 12/13/13 04:32 PM (10 years, 2 months ago)

Thanks for the info. It's funny, I've read so many conflicting things from RR over the years as information changes, but by the nature of the forum he'll often just state things without reasoning just as fact alone leaving you to question why he said it in the first place, whether it was from research, observation, or often whether it's even a fact or an opinion in many contexts.

Over the years as I've learned everything this lead to a period of time where I just felt like I didn't know anything because I had so much conflicting junk filling up my brain. Also due to the nature of being a forum and the way the search engine works these old or outdated ideas are still often brought up to just further add to the confusion. There also doesn't seem to be much in the way of good mycology books or resources out there, many of which have misinformation anyhow. Mycology as a result, at least for the hobbyist, seems much less a science and more of an art in that you kind of just have to try it for yourself to see what does what, and it may well do the opposite for someone else.


Extras: Filter Print Post Top
Offline1down5up
Social Ninja
Male User Gallery

Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
Re: Slow Colonization... What To Do? [Re: krypto2000]
    #19271573 - 12/13/13 04:40 PM (10 years, 2 months ago)

Most professionals never stop learning, which means they have no problem re-writing info if they believe it to be redundant.  I hope RR keeps re-writing everything and shares it all here....it will only benefit us all...and if he were to try and explain everything he knows to me, my fuckin head would explode...


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


Edited by 1down5up (12/13/13 04:41 PM)


Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: Slow Colonization... What To Do? [Re: 1down5up]
    #19271644 - 12/13/13 04:59 PM (10 years, 2 months ago)

I'd read RR's book :thumbup:


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
Trusted Cultivator
Re: Slow Colonization... What To Do? [Re: krypto2000]
    #19271650 - 12/13/13 05:01 PM (10 years, 2 months ago)

Quote:

krypto2000 said:
I'd read RR's book :thumbup:



until then
http://www.shroomery.org/forums/showflat.php/Number/8468463#8468463:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineNorCalCaps
Stranger

Registered: 11/20/07
Posts: 15
Last seen: 9 years, 4 months
Re: Slow Colonization... What To Do? [Re: cronicr]
    #19275112 - 12/14/13 03:02 PM (10 years, 2 months ago)

I can't speak to the nature of RR's info, but what krypto2000 said rings true with a newbie like me. Anyone here can state anything, and a moment later I can find something that totally conflicts with that information.

I have already learned much in this thread and am glad I posted. I am feeling better about the slow migration now that I broke up my substraight.

I mentioned above in this thread that 2 of my colonies are showing some light brown coloring within the mycelium. Should I be concerned? Or is this normal?


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
Trusted Cultivator
Re: Slow Colonization... What To Do? [Re: NorCalCaps]
    #19275127 - 12/14/13 03:05 PM (10 years, 2 months ago)

i would post a pic, doesn't sound right


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Kratom Capsules for Sale   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* Slow colonization.
( 1 2 all )
u4ia 5,994 23 05/13/03 06:14 PM
by u4ia
* b+ slow colonizer??? novicegrower185 2,875 7 10/25/02 05:26 AM
by mickey_rourke
* PESA = slow colonizer merlink 939 1 08/29/03 01:35 AM
by Psilocybin_monkey
* Lack of oxygen causing slow colonization? P2K1 1,615 8 03/24/04 08:26 PM
by simplemachine
* Slow colonization *DELETED* Grenade01 2,426 6 11/09/01 04:00 AM
by puscle
* slow colonization? hairs 1,674 1 01/08/02 02:43 PM
by Anonymous
* Very Slow Colonization peacefrog14 7,528 7 09/23/01 10:23 PM
by Zen Peddler
* Slow colonizing?? EckoEst 603 1 08/23/03 08:53 PM
by SubGen1us

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,355 topic views. 34 members, 231 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.007 seconds on 12 queries.