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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: You'll never guess, my question about cube cultivation [Re: cronicr]
#19655347 - 03/05/14 03:19 PM (9 years, 10 months ago) |
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i have one strain that NEVER germinates on agar. i have to noc up a grain jar then transfer a grain to agar or clone it. its in a syringe too, so they are not dry.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
#19655359 - 03/05/14 03:22 PM (9 years, 10 months ago) |
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send it my way i'll fix it up for ya
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
#19655360 - 03/05/14 03:22 PM (9 years, 10 months ago) |
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Quote:
blindingleaf said: i have one strain that NEVER germinates on agar. i have to noc up a grain jar then transfer a grain to agar or clone it. its in a syringe too, so they are not dry.
What strain?
I hope you did not jut call a group of spores a strain.....that would be.......noobish......lol.
A spore print/syringe is not a strain......2 spores make a strain, there are millions of spores in a print/syringe, so there are millions of strains in a print/syringe.
The name some vendor gave a certain group of spores is mostly a marketing gimic.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: You'll never guess, my question about cube cultivation [Re: PussyFart]
#19655364 - 03/05/14 03:23 PM (9 years, 10 months ago) |
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ur right my bad!  Peurto Rican
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
#19655370 - 03/05/14 03:24 PM (9 years, 10 months ago) |
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thats your nemisis variety huh lol!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
#19655371 - 03/05/14 03:24 PM (9 years, 10 months ago) |
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I bet I can get it to germinate on agar.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: You'll never guess, my question about cube cultivation [Re: PussyFart]
#19655376 - 03/05/14 03:25 PM (9 years, 10 months ago) |
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if it was a print i would send u a swipe!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
#19655393 - 03/05/14 03:28 PM (9 years, 10 months ago) |
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Most of the spores I've put on agar came out of a syringe. I think my real problem is that my no pour agar keeps boiling over in the PC creating a pathway for bacteria to get in and take over before my spores can germinate. I tried GWA for my last batch and it didn't even set up after PCing. It was more like pudding.
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
#19655840 - 03/05/14 04:55 PM (9 years, 10 months ago) |
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Two of four of my sponsor syringes were the same way. I pulled a bubble in one and finally got it on grain. I noc'd three dishes twice and nothing and two grains twice nothing. The other syringe I just put up but did 2 dishes twice with it and nothing. The other two syringes from same sponsor had growth in two days on agar and grains.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: You'll never guess, my question about cube cultivation [Re: Happy Littletree]
#19655877 - 03/05/14 05:04 PM (9 years, 10 months ago) |
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My syringes are all home made and they usually germinate on grain in 3 or 4 days.
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
#19655928 - 03/05/14 05:16 PM (9 years, 10 months ago) |
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I've never had an issue with boil over. I use ELMEA. I make sure my plates are all the way up out of the water. and sandwich them between two sheets of aluminum foil. 15psi for 45. I've used 1/4 pint glass and these pp5 plastic ones.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: You'll never guess, my question about cube cultivation [Re: Happy Littletree]
#19655969 - 03/05/14 05:25 PM (9 years, 10 months ago) |
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I've been PCing for between 90 and 120 minutes could this be my problem. I should mention my PC only gets up to 11.6 PSI.
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
#19655979 - 03/05/14 05:27 PM (9 years, 10 months ago) |
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Maybe try a small batch at 60 minutes.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: You'll never guess, my question about cube cultivation [Re: Happy Littletree]
#19656005 - 03/05/14 05:33 PM (9 years, 10 months ago) |
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Ill be doing that tonight as soon as my kids go to bed.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
#19656987 - 03/05/14 08:38 PM (9 years, 10 months ago) |
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you can PC agar for like 20M at 15PSI and be fine 98+% of the time. I would do like 30-40M with the 11psi and see how it works for you
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: You'll never guess, my question about cube cultivation [Re: bodhisatta]
#19657003 - 03/05/14 08:42 PM (9 years, 10 months ago) |
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I'm glad I read this poste I just mixed up some agar and I'm about to load my PC.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
#19657022 - 03/05/14 08:44 PM (9 years, 10 months ago) |
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I personally PSI my agar at 15psi for like 25-30M so you should be just fine at 30-40. You can sterilize agar without a PC in a steam bath(idk how long it takes though but it's not outrageously long) since there's no hard to kill bacterial endospores and easy heat transfer. I think you'll be just fine.
Edited by Trusted cuItivator (03/05/14 08:45 PM)
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: You'll never guess, my question about cube cultivation [Re: bodhisatta]
#19657042 - 03/05/14 08:48 PM (9 years, 10 months ago) |
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Sweet thanx.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
#19658957 - 03/06/14 10:59 AM (9 years, 10 months ago) |
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the difference between 10 and fifteen psi really isn't all that much
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: You'll never guess, my question about cube cultivation [Re: cronicr]
#19658966 - 03/06/14 11:04 AM (9 years, 10 months ago) |
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I went with 45 minutes and it worked out a lot better than my previous batches. No boil over and very little moisture on the walls of my PP5s. I just made a ghetto inoculation loop out of twist ties and hopefully I can get some spores to germinate this time.
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