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Invisibleblindingleaf
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Re: You'll never guess, my question about cube cultivation [Re: cronicr]
    #19655347 - 03/05/14 03:19 PM (9 years, 10 months ago)

i have one strain that NEVER germinates on agar.  i have to noc up a grain jar then transfer a grain to agar or clone it.  its in a syringe too, so they are not dry.


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OfflinecronicrFacebook
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
    #19655359 - 03/05/14 03:22 PM (9 years, 10 months ago)

send it my way i'll fix it up for ya:wink:


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OfflinePussyFart
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
    #19655360 - 03/05/14 03:22 PM (9 years, 10 months ago)

Quote:

blindingleaf said:
i have one strain that NEVER germinates on agar.  i have to noc up a grain jar then transfer a grain to agar or clone it.  its in a syringe too, so they are not dry.



What strain?

I hope you did not jut call a group of spores a strain.....that would be.......noobish......lol.

A spore print/syringe is not a strain......2 spores make a strain, there are millions of spores in a  print/syringe, so there are millions of strains in a print/syringe.

The name some vendor gave a certain group of spores is mostly a marketing gimic.


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Invisibleblindingleaf
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Re: You'll never guess, my question about cube cultivation [Re: PussyFart]
    #19655364 - 03/05/14 03:23 PM (9 years, 10 months ago)

ur right my bad!  :facepalm:
Peurto Rican


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinecronicrFacebook
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
    #19655370 - 03/05/14 03:24 PM (9 years, 10 months ago)

thats your nemisis variety huh lol!


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I'm tired do me a favor


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OfflinePussyFart
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
    #19655371 - 03/05/14 03:24 PM (9 years, 10 months ago)

I bet I can get it to germinate on agar.


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Invisibleblindingleaf
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Re: You'll never guess, my question about cube cultivation [Re: PussyFart]
    #19655376 - 03/05/14 03:25 PM (9 years, 10 months ago)

if it was a print i would send u a swipe!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleMudaFuka
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Re: You'll never guess, my question about cube cultivation [Re: blindingleaf]
    #19655393 - 03/05/14 03:28 PM (9 years, 10 months ago)

Most of the spores I've put on agar came out of a syringe. I think my real problem is that my no pour agar keeps boiling over in the PC creating a pathway for bacteria to get in and take over before my spores can germinate. I tried GWA for my last batch and it didn't even set up after PCing. It was more like pudding.


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OfflineHappy Littletree
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
    #19655840 - 03/05/14 04:55 PM (9 years, 10 months ago)

Two of four of my sponsor syringes were the same way. I pulled a bubble in one and finally got it on grain. I noc'd three dishes twice and nothing and two grains twice nothing. The other syringe I just put up but did 2 dishes twice with it and nothing. The other two syringes from same sponsor had growth in two days on agar and grains.


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InvisibleMudaFuka
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Re: You'll never guess, my question about cube cultivation [Re: Happy Littletree]
    #19655877 - 03/05/14 05:04 PM (9 years, 10 months ago)

My syringes are all home made and they usually germinate on grain in 3 or 4 days.


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OfflineHappy Littletree
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
    #19655928 - 03/05/14 05:16 PM (9 years, 10 months ago)

I've never had an issue with boil over. I use ELMEA. I make sure my plates are all the way up out of the water. and sandwich them between two sheets of aluminum foil. 15psi for 45. I've used 1/4 pint glass and these pp5 plastic ones.


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InvisibleMudaFuka
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Re: You'll never guess, my question about cube cultivation [Re: Happy Littletree]
    #19655969 - 03/05/14 05:25 PM (9 years, 10 months ago)

I've been PCing for between 90 and 120 minutes could this be my problem. I should mention my PC only gets up to 11.6 PSI.


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OfflineHappy Littletree
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
    #19655979 - 03/05/14 05:27 PM (9 years, 10 months ago)

Maybe try a small batch at 60 minutes. :shrug:


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InvisibleMudaFuka
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Re: You'll never guess, my question about cube cultivation [Re: Happy Littletree]
    #19656005 - 03/05/14 05:33 PM (9 years, 10 months ago)

Ill be doing that tonight as soon as my kids go to bed.


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InvisiblebodhisattaMDiscordReddit
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
    #19656987 - 03/05/14 08:38 PM (9 years, 10 months ago)

you can PC agar for like 20M at 15PSI and be fine 98+% of the time. I would do like 30-40M with the 11psi and see how it works for you


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InvisibleMudaFuka
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Re: You'll never guess, my question about cube cultivation [Re: bodhisatta]
    #19657003 - 03/05/14 08:42 PM (9 years, 10 months ago)

I'm glad I read this poste I just mixed up some agar and I'm about to load my PC.


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InvisiblebodhisattaMDiscordReddit
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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
    #19657022 - 03/05/14 08:44 PM (9 years, 10 months ago)

I personally PSI my agar at 15psi for like 25-30M so you should be just fine at 30-40. You can sterilize agar without a PC in a steam bath(idk how long it takes though but it's not outrageously long) since there's no hard to kill bacterial endospores and easy heat transfer. I think you'll be just fine.


Edited by Trusted cuItivator (03/05/14 08:45 PM)


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InvisibleMudaFuka
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Re: You'll never guess, my question about cube cultivation [Re: bodhisatta]
    #19657042 - 03/05/14 08:48 PM (9 years, 10 months ago)

Sweet thanx.


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Re: You'll never guess, my question about cube cultivation [Re: MudaFuka]
    #19658957 - 03/06/14 10:59 AM (9 years, 10 months ago)

the difference between 10 and fifteen psi really isn't all that much:cool:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleMudaFuka
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Re: You'll never guess, my question about cube cultivation [Re: cronicr]
    #19658966 - 03/06/14 11:04 AM (9 years, 10 months ago)

I went with 45 minutes and it worked out a lot better than my previous batches. No boil over and very little moisture on the walls of my PP5s. I just made a ghetto inoculation loop out of twist ties and hopefully I can get some spores to germinate this time.


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