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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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SizlChest
Padawan



Registered: 09/29/13
Posts: 814
Loc: Texas
Last seen: 2 hours, 42 minutes
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Re: The journey to find my system [Re: Stromrider]
#19312464 - 12/22/13 03:56 PM (10 years, 1 month ago) |
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Quote:
Stromrider said:
Quote:
BigGreenMat said: How are you doing your agar? Making in bottle in a PC then pouring into plastic dishes in SAB?
I do believe you answered your own question 
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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Re: The journey to find my system [Re: SizlChest]
#19312850 - 12/22/13 05:43 PM (10 years, 1 month ago) |
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Hey just checking! Sizl has been doing so well I wanted to check to see if we are doing the same teks. I did some agar at work, but going forward probably can't often so want to make some at home
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: The journey to find my system [Re: BigGreenMat]
#19312861 - 12/22/13 05:46 PM (10 years, 1 month ago) |
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Quote:
BigGreenMat said: , but going forward probably can't often so want to make some at home
whoa, am i that fucked today or does that make no sense at all
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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Re: The journey to find my system [Re: cronicr]
#19312894 - 12/22/13 05:54 PM (10 years, 1 month ago) |
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Both cronicr. I was trying to say o probably won't be able to make agar at work often in the future so i will have to at home
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: The journey to find my system [Re: BigGreenMat]
#19312907 - 12/22/13 05:57 PM (10 years, 1 month ago) |
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thnx for clearing that up, and also clearing up wut you meant
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SizlChest
Padawan



Registered: 09/29/13
Posts: 814
Loc: Texas
Last seen: 2 hours, 42 minutes
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Re: The journey to find my system [Re: cronicr]
#19324818 - 12/25/13 08:37 AM (10 years, 1 month ago) |
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Some agar updates:
These are from the first donor mushie. I am going to isolate some sectors tonight.









These are my multi spore plates. Lots of isolating to do with these:

The second run of clones I lost 5 plates to contams. The sleeve of petri dishes wasn't tight to the tube of dishes....probably air got in there?? The plastic didn't have that vacuum-sealed look to it. Bright circles of orange, and cloudy grey liquid was is all five of the contaminated dishes. I immediately chucked them.
My grain master jar is looking to be 85% colonized, after I shook it 5 days ago at roughly 30% colonization. I plan to do gtg transfers, and make 10 jars of spawn. This is a multi spore run, so I will be taking tissue from the good performers of this tub, also. In 3 months, I plan on having 1 or 2 isolates that I will keep growing out.
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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bluecap
mychanical



Registered: 11/10/13
Posts: 286
Last seen: 8 years, 4 months
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Re: The journey to find my system [Re: SizlChest]
#19324920 - 12/25/13 09:16 AM (10 years, 1 month ago) |
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wow, nice job there! looks like you have some good monocultures already, from that first clone. have you started test grows yet with these? damn that's a lot to work with. bet the grows will be great from those...I would even leave a couple intact, with different sectors growing together, just for fun....your havin fun now!...peace...
-------------------- I wish I was a grain of sand, layin in a babies hand...fallin like a diamond chain into the ocean... A willow tree is strong enough to bend, never like the oak who lives in fear of a wind....Gamma... 
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 18 hours, 5 minutes
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Re: The journey to find my system [Re: bluecap]
#19325066 - 12/25/13 10:21 AM (10 years, 1 month ago) |
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Looking good
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BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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Re: The journey to find my system [Re: Stromrider]
#19325091 - 12/25/13 10:30 AM (10 years, 1 month ago) |
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Yeah some of those first clones look very isolated already! Very nice pictures!
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SizlChest
Padawan



Registered: 09/29/13
Posts: 814
Loc: Texas
Last seen: 2 hours, 42 minutes
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Re: The journey to find my system [Re: BigGreenMat]
#19348644 - 12/30/13 09:12 PM (10 years, 1 month ago) |
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UPDATE"
So, I took some sectors from the C1 clones, and they are all showing growth.

I used the original agar dishes I took the samples from to inoculate 10 grain jars, and I'm planning on doing a tub with these to test potency and performance.
Tissue from C2 clone is doing well, and my next project is to take sectors from these, then use wedges to inoculate my next run of grain jars.

As always, please be brutal with your criticism, thanks all!
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: The journey to find my system [Re: SizlChest]
#19348651 - 12/30/13 09:13 PM (10 years, 1 month ago) |
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Quote:
SizlChest said:
As always, please be brutal with your criticism, thanks all!
i couldn't even find a spelling mistake
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SizlChest
Padawan



Registered: 09/29/13
Posts: 814
Loc: Texas
Last seen: 2 hours, 42 minutes
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Re: The journey to find my system [Re: cronicr]
#19348657 - 12/30/13 09:15 PM (10 years, 1 month ago) |
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OOPS, here are the grain jars:
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: The journey to find my system [Re: cronicr]
#19348662 - 12/30/13 09:17 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
SizlChest said:
As always, please be brutal with your criticism, thanks all!
i couldn't even find a spelling mistake
your picture isn't level, try less coffee in the morning
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SizlChest
Padawan



Registered: 09/29/13
Posts: 814
Loc: Texas
Last seen: 2 hours, 42 minutes
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Re: The journey to find my system [Re: cronicr]
#19348669 - 12/30/13 09:18 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
SizlChest said:
As always, please be brutal with your criticism, thanks all!
i couldn't even find a spelling mistake
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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SizlChest
Padawan



Registered: 09/29/13
Posts: 814
Loc: Texas
Last seen: 2 hours, 42 minutes
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Re: The journey to find my system [Re: SizlChest]
#19366969 - 01/04/14 05:56 AM (10 years, 27 days ago) |
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Here's a bit of an update. My first grain jars inoculated with agar wedges from my first isolate are looking pretty good, so far. Nice growth.

Question, next time I do this, should I shake the jar a bit to get the agar down into the jar, or just lay it on top? Or, does it not even matter?
Here's a pic of my second grain jars with isolate number two. I just inoculated two days ago with agar wedges, also. This isolate has four more dishes that I need to transfer to. The mycellium has almost grown over the entire surface of the dish.

Here's the transfers from C1:

I have two tubs that are the recipients of my final cakes (at least for a while) and I will select tissue from good performers to clone and isolate. I am up to 20 grain jars sitting on my shelf, and I am contemplating buying another couple cases so I can keep it going.....this is addictive!
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: The journey to find my system [Re: SizlChest]
#19367056 - 01/04/14 06:53 AM (10 years, 27 days ago) |
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i like ur method of covering all bases at once! agar, g2g'ing, cloning, ms agar, monos, etc. i like to have a lot going at one time too, less large grows, more small ones till i find just the right strain, substrate, etc. keeps me busy and out of trouble, kind of obsessive (and addicting like u said) but i don't spend my money on anything else (anymore...)...so 
some of those clones plates look really good, maybe already an iso...? i can't zoom because the site it takes me to when i click it does not load properly
have u tried filter bags yet? i just did 2 the other day, most likely one is messed up (they take along time to cool, and i didn't realize that before dumping a qt of spawn in) but it may help avoid costs/space/time with jars. thats y i am beginning to experiment with them anyways..
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: The journey to find my system [Re: blindingleaf]
#19367701 - 01/04/14 11:34 AM (10 years, 26 days ago) |
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yeah those clones are looking yummy, great stuff to see and yes always do more jars...always
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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Re: The journey to find my system [Re: cronicr]
#19368113 - 01/04/14 12:56 PM (10 years, 26 days ago) |
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Linking great sizl. I need to take pictures!
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SizlChest
Padawan



Registered: 09/29/13
Posts: 814
Loc: Texas
Last seen: 2 hours, 42 minutes
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Re: The journey to find my system [Re: BigGreenMat]
#19469249 - 01/24/14 07:59 PM (10 years, 6 days ago) |
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UPDATE:
Guess what I just got finished doing? Inoculating grain masters with agar wedges, and isolating sectors for the second time from some MS plates I grew. I have a good isolate of C1 going, and the tub was just put into fruiting today. C2 had a lot of sectors that needed isolating, and I have done 2 runs of isolating, and tonight I did the G2G for my next mono run. HAve fun, and I promise there will be some pics next time it's updated.
-------------------- PrimalSoup's Tea Tek
   "I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!" "Hell, shrooms have blown up from under me and kicked my ass on 2 grams once." "I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: The journey to find my system [Re: SizlChest]
#19470811 - 01/25/14 06:54 AM (10 years, 6 days ago) |
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lol dude i feel like I'm watching scrubs.
i dig the purple gloves. i use clear b/c i take them from my food service job.
its ball-sy to put a pic up of urself (if indeed that is you).
good luck, keep us updated!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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