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nomadbrad
Oregrownian



Registered: 10/23/06
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I poured plates for the first time, need some ID's
#19266603 - 12/12/13 04:13 PM (10 years, 1 month ago) |
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As the title states I jumped into agar work. I now have some questions about what's growing in them.
P.serbica from spores. What's the discoloration in the middle?
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Oyster transfer.

P.baeocystis from spores. Is that healthy myc?

I don't know really what to expect here but I think the serbica has some contams but I'm not really sure. Thanks for the help!
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19266790 - 12/12/13 05:02 PM (10 years, 1 month ago) |
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Looks like it may be a contaminant from another species of fungus on the serbica.
Also there is a contaminant next to your oyster culture.
The baeo looks alright.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
Edited by Ganzig (12/12/13 05:10 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19266824 - 12/12/13 05:09 PM (10 years, 1 month ago) |
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the baeo looks about right, can't tell ya much about the others but maybe pm psilosimon
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19267032 - 12/12/13 06:01 PM (10 years, 1 month ago) |
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Quote:
Ganzig said: Looks like it may be a contaminant from another species of fungus on the serbica.
Also there is a contaminant next to your oyster culture.
The baeo looks alright.
Yeah your probably right. It's just weird how both spots are right where clumps of spores are.
Do you think I could isolate away from the contams?
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: cronicr]
#19267037 - 12/12/13 06:03 PM (10 years, 1 month ago) |
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Quote:
cronicr said: the baeo looks about right, can't tell ya much about the others but maybe pm psilosimon
Thanks man, I might just do that.
I knew my MH&I buddies would have my back!
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19267043 - 12/12/13 06:04 PM (10 years, 1 month ago) |
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I think it makes perfect sense that the contams are right where you put a bunch of wild collection spores.
This one might not be a win. The mycelium of serbica is weak.
You might as well wait to see what happens. How many plates of serbica did you make?
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19267065 - 12/12/13 06:09 PM (10 years, 1 month ago) |
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I made two plates of serbica and I still have a print to work with. The second plate has no action yet.
I will make a few more tomorrow I think. Man it looked great until that color showed up!
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19267119 - 12/12/13 06:22 PM (10 years, 1 month ago) |
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How long have you had them on agar? I would be weary about anything that was faster than about a week.
Serbica is slow on top of being weak.
I don't know from experience. I had some barely alive given to me and tried to save it but trich had other plans.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19267243 - 12/12/13 06:50 PM (10 years, 1 month ago) |
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They were knocked up about 10 pm last Saturday. Granted I was drinking whiskey to stay warm in a frigid lab, I tried to be as careful as possible. All of my control plates are still clean.
If the white rhizos continue to run past the colored middle spot I will isolate it and see what species it is at least?!?
Serbica being slow and weak. Is that typical from experience of others or is that just a widely known fact?
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19267255 - 12/12/13 06:52 PM (10 years, 1 month ago) |
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Isolating the growth just to make sure is a good idea.
Slow growth was the experience of a friend of mine.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
Edited by Ganzig (12/12/13 06:53 PM)
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19267312 - 12/12/13 07:01 PM (10 years, 1 month ago) |
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I'm thinking so too and I love thinking I'm a scientist while using a scalpel so it's an excuse to play out my nerd fantasy. 
Hopefully If it is indeed serbica, I can isolate it and have some faster growing genetics. Yay!
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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,146
Loc: North/Western WA
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19269244 - 12/13/13 03:14 AM (10 years, 1 month ago) |
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Nice work and enthusiasm. Keep it up.
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mushrume man
Sadis Factory



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Re: I poured plates for the first time, need some ID's [Re: NothingsChanged]
#19309674 - 12/21/13 09:51 PM (10 years, 1 month ago) |
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Would streaking the wild spores across the plate give more chance for mycelium to pop out, among the contams, and be isolated?
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: I poured plates for the first time, need some ID's [Re: mushrume man]
#19309725 - 12/21/13 10:11 PM (10 years, 1 month ago) |
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do you mean isolated away from contams or strain isolations? don't isolate strains for anything that takes that long and goes outdoors theres no way to test it but yes i streak mine to isolate away from contams, hows about an update?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
Edited by cronicr (12/21/13 11:40 PM)
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mushrume man
Sadis Factory



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Re: I poured plates for the first time, need some ID's [Re: cronicr]
#19310115 - 12/22/13 12:34 AM (10 years, 1 month ago) |
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Yea I meant away from contams
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: mushrume man] 1
#19313654 - 12/22/13 08:57 PM (10 years, 1 month ago) |
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UPDATE!
The above plate got out of control too fast but the second P.serbica plate germinated and I was able to do two transfers from it to new plates. They are doing well with nice rhizos coming outl. There is a few satellite colonies on one so I will have to do another set of transfers. What do you think?
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19313895 - 12/22/13 10:01 PM (10 years, 1 month ago) |
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Nice!
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19313901 - 12/22/13 10:05 PM (10 years, 1 month ago) |
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Fucking beautiful!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MAIA
World-BridgerKartikeya (DftS)



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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19317593 - 12/23/13 06:21 PM (10 years, 1 month ago) |
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Very good !!!
-------------------- Spiritual being, living a human experience ... The Shroomery Mandala
 Use, do not abuse; neither abstinence nor excess ever renders man happy. Voltaire
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nomadbrad
Oregrownian



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Posts: 1,160
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Re: I poured plates for the first time, need some ID's [Re: MAIA]
#19327288 - 12/25/13 11:33 PM (10 years, 1 month ago) |
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Might as well document the progress, maybe I should ask to have this moved to grow logs. Anyways...
I noticed some bacterial contams on both of the transfer plates so I decided I better act quick. I might 5 transfers total. Notice how the master plate (the one with the streak) is not contam'd and looks really healthy. It's got a lot of genetics going on.
Transfer 1

Transfer 2

Master plate looking good!

Merging

Hopefully I'm going in the right direction here.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19328395 - 12/26/13 11:14 AM (10 years, 1 month ago) |
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Looks like your doing fine to me, keep me posted on the baeos too
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
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Re: I poured plates for the first time, need some ID's [Re: cronicr]
#19328574 - 12/26/13 12:08 PM (10 years, 1 month ago) |
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Well sad news about the Baeo plates....the only spores to germinate were not Baeo spores. One of the plates didn't germinate anything, just has a clean streak of purple brown spores. I was told the print was over a year old, I don't know how to breath life back into them. I'm wondering if rehydrating the spores before I swipe the plates might help.
A fresh print would be good too...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19328582 - 12/26/13 12:11 PM (10 years, 1 month ago) |
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I like the no pour method proffesor pin head posted for old spores prints,, I didn't have any kuck with my one bae print this year either
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
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Re: I poured plates for the first time, need some ID's [Re: cronicr]
#19328615 - 12/26/13 12:22 PM (10 years, 1 month ago) |
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Whaaaa what what?! I'd like to see this. Can you link me up?
People were not joking when they said they were hard to cultivate....
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19328739 - 12/26/13 12:52 PM (10 years, 1 month ago) |
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On my tab and got no clue how to post links with it but type no pour into the search bar, I like it cause I can hydrate sores in a syringe before I inoculate
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19328839 - 12/26/13 01:10 PM (10 years, 1 month ago) |
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Quote:
nomadbrad said: Well sad news about the Baeo plates....the only spores to germinate were not Baeo spores. One of the plates didn't germinate anything, just has a clean streak of purple brown spores. I was told the print was over a year old, I don't know how to breath life back into them. I'm wondering if rehydrating the spores before I swipe the plates might help.
A fresh print would be good too...
Ask Joust for a new print.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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NothingsChanged
Striving for Excellence


Registered: 05/28/11
Posts: 10,146
Loc: North/Western WA
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19331737 - 12/27/13 04:40 AM (10 years, 1 month ago) |
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Quote:
nomadbrad said: Well sad news about the Baeo plates....the only spores to germinate were not Baeo spores. One of the plates didn't germinate anything, just has a clean streak of purple brown spores. I was told the print was over a year old, I don't know how to breath life back into them. I'm wondering if rehydrating the spores before I swipe the plates might help.
...
Shit that sucks. It never stops being a learning experiance. Bonus.
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: NothingsChanged]
#19454957 - 01/21/14 09:18 PM (10 years, 9 days ago) |
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So update on baeos.
I tried again with spores, some germinated so I transferred the strongest leading edge and the only one to take so far looks like this.
Is that blue from some kind if contam or psilocybin?
My guess is contam.

Also, serbica will be on grain by next weekend. I've been battling bacteria and I think I've got the upper hand now phew...
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19454969 - 01/21/14 09:22 PM (10 years, 9 days ago) |
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That blue looks like a psilocybe blue. 
Can you take that picture with natural light tomorrow?
That would be cool.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19454977 - 01/21/14 09:25 PM (10 years, 9 days ago) |
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I can do that for sure. If it is psilocybin I'm going to pee myself.
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19458009 - 01/22/14 03:03 PM (10 years, 8 days ago) |
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I woke up to this.... Noooooooooooo! 


Even on my oyster in vitro pin transfer
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19458112 - 01/22/14 03:22 PM (10 years, 8 days ago) |
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Bummer dude. Looks like you were transferring mold.
You could save that oyster culture.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19458142 - 01/22/14 03:28 PM (10 years, 8 days ago) |
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Thanks for pointing that out so clearly 
I will save the oysters at once! Well after I shower and re sterilize my whole lab after my mold transfers I performed.
This agar deal is driving me nuts in a glove box. I want to be able to see better when using the scalpel. I need a laminar badly.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19458214 - 01/22/14 03:45 PM (10 years, 8 days ago) |
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I made my flow hood for about 300. If you have wood lying around it will be even cheaper! Sorry for pouring salt in the wound.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 3 months
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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19458248 - 01/22/14 03:51 PM (10 years, 8 days ago) |
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No worries, I'll refine my technique more and more. 
I've got all the stuff to make it except a filter and a blower.
http://www.shroomery.org/forums/showflat.php/Number/19375349/fpart/2/vc/1#19375349
Think 250 is too much for the blower and filter? Do you know if used filters are even worth it?
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: nomadbrad]
#19458627 - 01/22/14 04:50 PM (10 years, 8 days ago) |
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I bought this filter from these folks here. $100 and it has been working just fine for a year and a half.
They were good to me. The first filter they sent was damaged and they just sent me a second one that same day without paperwork or anything.
http://www.ebay.com/itm/Astrocel-1-Military-Nuclear-Grade-HEPA-Filters-/301052813722?pt=LH_DefaultDomain_0&hash=item461825619a
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19458640 - 01/22/14 04:53 PM (10 years, 8 days ago) |
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I bought my blower from some guy on craigslist for $30.
It's up to you mang.
edit- I would just start from scratch. That filter looks a little beat up.
Probably pretty used.
I'd get yourself a new one.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
Edited by Ganzig (01/22/14 04:55 PM)
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nomadbrad
Oregrownian



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Re: I poured plates for the first time, need some ID's [Re: Ganzig]
#19458755 - 01/22/14 05:17 PM (10 years, 8 days ago) |
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Wow nice! That's the cheapest I've seen them. I'm stoked now.
Thanks for the advice I think I'll go new
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