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OfflineKingCloyster
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The piece of agar i used for transfer isnt colonzing, unlike the rest of the agar... Contam?
    #19265748 - 12/12/13 01:11 PM (10 years, 1 month ago)

Ive been storing some isolates in slants lately, all seeming to have very healthy, sexy rhizo growth throughout the tube. But when I look at the tiny piece of agar i used to transfer the mycelium, it doesnt look like thick rhizogrowth, its way thinner, not exposed, but thinner and I can see the agars darkness through the mycelium easily. it im just confused why some didnt colonize as thick as the rest of the agar did.  is it a small contam?


Edited by KingCloyster (12/12/13 05:57 PM)


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OfflineKingCloyster
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Re: All of the slant is colonized except for the piece of agar i used for transfer... Contam? [Re: KingCloyster]
    #19266723 - 12/12/13 04:45 PM (10 years, 1 month ago)




Edited by KingCloyster (12/12/13 04:51 PM)


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OfflineKingCloyster
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Re: All of the slant is colonized except for the piece of agar i used for transfer... Contam? [Re: KingCloyster]
    #19266726 - 12/12/13 04:46 PM (10 years, 1 month ago)

These are the two that I'm seeing this happen in... *Located in the center of the pics


Edited by KingCloyster (12/12/13 07:24 PM)


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OfflineKingCloyster
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Registered: 05/27/13
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Re: All of the slant is colonized except for the piece of agar i used for transfer... Contam? [Re: KingCloyster]
    #19267468 - 12/12/13 07:25 PM (10 years, 1 month ago)

Bump


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OfflineKingCloyster
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Registered: 05/27/13
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Re: All of the slant is colonized except for the piece of agar i used for transfer... Contam? [Re: KingCloyster]
    #19273958 - 12/14/13 09:57 AM (10 years, 1 month ago)

No love :confused:


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OfflineMAIA
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Re: All of the slant is colonized except for the piece of agar i used for transfer... Contam? [Re: KingCloyster]
    #19276465 - 12/14/13 08:28 PM (10 years, 1 month ago)

This is a hard one and you probably should get a better answer in advanced myc but I'll give it a shot ... what's in the agar mixture and how was did you process it  ?

Btw, can you please take some pics further away ? I can't understand what's happening in those pics besides the obvious.


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