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OfflineBOOMERang27
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Registered: 12/09/13
Posts: 12
Last seen: 9 years, 11 months
uneven inoculation
    #19264703 - 12/12/13 08:10 AM (10 years, 1 month ago)

I just finished inoculating my BRF jars. A few of the jars did not receive exactly 1cc of spore solution (under and over in some cases), and in others the 1cc was not distributed evenly. This was my first time and my hands are shakey. How will this effect the colonization?


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Offlinejimmyjame1
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Re: uneven inoculation [Re: BOOMERang27]
    #19264723 - 12/12/13 08:15 AM (10 years, 1 month ago)

yeah, you still injected thousands of spores into those jars. your gonna be fine.


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OfflineKnate420
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Re: uneven inoculation [Re: jimmyjame1]
    #19264767 - 12/12/13 08:24 AM (10 years, 1 month ago)

Being more precise with inoculations usually results in uniform colonization, and therefore faster colonization IME.


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OfflineBOOMERang27
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Registered: 12/09/13
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Last seen: 9 years, 11 months
Re: uneven inoculation [Re: jimmyjame1]
    #19264785 - 12/12/13 08:30 AM (10 years, 1 month ago)

Quote:

jimmyjame1 said:
yeah, you still injected thousands of spores into those jars. your gonna be fine.




Well good, thats a load off my mind. I was worried I had screwed up my whole tek before it even began!


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Invisiblepablokabute
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Registered: 11/22/11
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Re: uneven inoculation [Re: BOOMERang27]
    #19264871 - 12/12/13 08:55 AM (10 years, 1 month ago)

in some cases, bro, 0.5cc will do... (that is when the spores are fresh enough to germinate)

how many days has it been?


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OfflineBOOMERang27
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Registered: 12/09/13
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Last seen: 9 years, 11 months
Re: uneven inoculation [Re: pablokabute]
    #19264881 - 12/12/13 09:01 AM (10 years, 1 month ago)

Like 4 hours haha


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OfflineTheWiz
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Registered: 11/21/11
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Loc: Southern IL
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Re: uneven inoculation [Re: BOOMERang27]
    #19264920 - 12/12/13 09:12 AM (10 years, 1 month ago)

Quote:

BOOMERang27 said:
How will this effect the colonization?



Very, very little.  Don't sweat it.


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Invisibleawakendone
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Registered: 08/05/10
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Re: uneven inoculation [Re: BOOMERang27]
    #19264954 - 12/12/13 09:23 AM (10 years, 1 month ago)

i never even followed the 1cc per jar when i did cakes. as soon as i would see a drop of spore solution come out of the syringe tip i would move on to the next hole. ime 1cc per jar is way more than necessary! with fresh spores all it takes is literally a single drop per hole. when it comes to spores i fully subscribe to the concept that "less is more".


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OfflineNotfromarkansas
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Registered: 11/11/13
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Re: uneven inoculation [Re: awakendone]
    #19265003 - 12/12/13 09:39 AM (10 years, 1 month ago)

Ya i usually only use one of the self healing injection spots but my last jar took a week and two days to completely turn white. Fruiting is where shit starts to go down in my opinion


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OfflineBOOMERang27
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Registered: 12/09/13
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Re: uneven inoculation [Re: Notfromarkansas]
    #19265694 - 12/12/13 12:57 PM (10 years, 1 month ago)

How long does it usualy take for the mycelium to fully colonize?


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