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OfflineKalypto
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How hard is it to find and culture ergot?
    #19249040 - 12/09/13 07:42 AM (10 years, 2 months ago)

Just curious how hard it is to culture and more specifically how hard is it to find when leaving grain to rot to find it?


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Offlineshopdropper
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Re: How hard is it to find and culture ergot? [Re: Kalypto]
    #19249480 - 12/09/13 10:43 AM (10 years, 2 months ago)

it dosnet grow on rotting grain. it grows on flowering grain. its more of a mutual relationship, it actually dosent hurt the host plant that much at all. and depending on what Claviceps your looking for will determine where youll look.  if you live in an area where ergot is common, I'd start looking when the host plant is starting to flower and look for sticky secretions on the grain heads. or go looking around harvest time for the sclerotia


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OfflineRogerRabbitM
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Re: How hard is it to find and culture ergot? [Re: shopdropper]
    #19254177 - 12/10/13 07:19 AM (10 years, 2 months ago)

If you're in an area where grains can grow, plant a 50 pound bag or two of organic rye berries and see what happens.
RR



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InvisibleHashbuble
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Re: How hard is it to find and culture ergot? [Re: RogerRabbit]
    #19254185 - 12/10/13 07:22 AM (10 years, 2 months ago)

If your trying to make some L, I'd get some ergotamine in Mexico, try Tijuana.


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OfflineRogerRabbitM
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Re: How hard is it to find and culture ergot? [Re: Hashbuble]
    #19254210 - 12/10/13 07:36 AM (10 years, 2 months ago)

Not if you're driving a car with CA plates.  You'll suffer an armed robbery at the hands of the Tijuana Police.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleHashbuble
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Re: How hard is it to find and culture ergot? [Re: RogerRabbit]
    #19254214 - 12/10/13 07:37 AM (10 years, 2 months ago)

Word up


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OfflineKalypto
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Re: How hard is it to find and culture ergot? [Re: Hashbuble]
    #19254283 - 12/10/13 08:11 AM (10 years, 2 months ago)

I have AZ plates will that be a problem?


How much did you pay for it?

I dont have a problem growing it , but I would prefer not to so I dont contaminate my house.

Can I grow them in AZ , I didnt think I could due to the lack of humidity here , I was planning on growing a patch maybe 2 square feet outside


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OfflineRogerRabbitM
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Re: How hard is it to find and culture ergot? [Re: Kalypto]
    #19254413 - 12/10/13 09:00 AM (10 years, 2 months ago)

Just run a sprinkler a time or two every day.

You better plan on at least 1000 square feet if you hope to get any sclerotia.  They don't form on most of the stalks. 
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlineshopdropper
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Re: How hard is it to find and culture ergot? [Re: RogerRabbit]
    #19254423 - 12/10/13 09:05 AM (10 years, 2 months ago)

hey RR will C. paspali grow on rye? I know it isn't its natural host but knowing the claviceps species infects open pollenated like rye... if it were intentionally infected would it take?


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DON'T TRY THIS AT HOME: no guarantees can be made about the accuracy of the information herein. The information dicussed in these posts is purely hypothetical, and for intelectual purposes only. Any similarity between internet chat and real life is pure coincidence.

:google:


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OfflineKalypto
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Re: How hard is it to find and culture ergot? [Re: RogerRabbit]
    #19254432 - 12/10/13 09:09 AM (10 years, 2 months ago)

Quote:

RogerRabbit said:
Just run a sprinkler a time or two every day.

You better plan on at least 1000 square feet if you hope to get any sclerotia.  They don't form on most of the stalks. 
RR




I was thinking I would try to go to someplace that might have ergot to find it


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OfflineRogerRabbitM
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Re: How hard is it to find and culture ergot? [Re: Kalypto]
    #19254454 - 12/10/13 09:18 AM (10 years, 2 months ago)

Quote:

hey RR will C. paspali grow on rye?




I don't know, sorry.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinethenilsmeister
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Re: How hard is it to find and culture ergot? [Re: RogerRabbit]
    #19263650 - 12/11/13 11:53 PM (10 years, 2 months ago)

It might prove difficult since it is slightly biotrophic, host specific, and has several life stages. It might help to infuse your agar with rye stigmas.


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OfflineKalypto
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Re: How hard is it to find and culture ergot? [Re: thenilsmeister]
    #19264554 - 12/12/13 07:19 AM (10 years, 2 months ago)

I read you cant culture it with agar


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Offlineshopdropper
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Re: How hard is it to find and culture ergot? [Re: Kalypto]
    #19266475 - 12/12/13 03:42 PM (10 years, 2 months ago)

both purpurea and paspali will grow on agar. paspali dosent like to sporulate on PDA but will form honeydew on specialized agar recipes, from what ive read. although infusing agar with stigmas sounds interesting. thanks for the idea!


--------------------
DON'T TRY THIS AT HOME: no guarantees can be made about the accuracy of the information herein. The information dicussed in these posts is purely hypothetical, and for intelectual purposes only. Any similarity between internet chat and real life is pure coincidence.

:google:


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