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gratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
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inoculating rye grain with liquid culture syringe...
#19262279 - 12/11/13 07:43 PM (10 years, 1 month ago) |
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i've been searching through the forums for a while now to see if its possible to inoculate rye grain directly with liquid culture... Is this possible and if so could some one send me a link to a thread that explains how, thanks...
-------------------- Everybody got their own trip as you walk down the strip Your life could be changed by a talk with a stranger Someone told me a story they said that he knew me He told me a rhyme that transcended all time How we shared the same space, another time, another place He spoke with a rush about how so many lives have been touched
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PussyFart
Retired Cultivation Extrodinaire



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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262296 - 12/11/13 07:45 PM (10 years, 1 month ago) |
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Yes, just treat it as any other inoculation.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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gratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
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Last seen: 10 years, 1 day
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Re: inoculating rye grain with liquid culture syringe... [Re: PussyFart]
#19262324 - 12/11/13 07:49 PM (10 years, 1 month ago) |
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so if i treat it like its a pf tek, obviously my syringe will not reach the rye since my qt jars are only filled 2/3 of the way, should i tip the jars to the side to inject directly into the rye on all four sides?
-------------------- Everybody got their own trip as you walk down the strip Your life could be changed by a talk with a stranger Someone told me a story they said that he knew me He told me a rhyme that transcended all time How we shared the same space, another time, another place He spoke with a rush about how so many lives have been touched
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SupaThaRipper
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262367 - 12/11/13 07:53 PM (10 years, 1 month ago) |
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Just stick the needle in the jar and aim for one of the sides. Shoot a little in... like 1ml or so. With lc id do just a couple jars at first to make sure iy isnt contaminated
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TheMustardTiger


Registered: 08/19/13
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262382 - 12/11/13 07:56 PM (10 years, 1 month ago) |
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Just inoculate the top and turn your grains lightly inside the jar. With the PF tek, your syringe has to penetrate the dry berm layer and the substrate. With grains this is not so.
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: inoculating rye grain with liquid culture syringe... [Re: SupaThaRipper]
#19262386 - 12/11/13 07:56 PM (10 years, 1 month ago) |
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heat-squirt-shake at 30
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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36fuckin5
Alchemycologist


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Loc: Diving into Mystical Territori...
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Re: inoculating rye grain with liquid culture syringe... [Re: cronicr]
#19262398 - 12/11/13 07:58 PM (10 years, 1 month ago) |
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Make sure to have a proper lid with a self-healing injection port and filtered gas exchange.
Inoculate 1-2 CC per quart through the injection port in your SAB.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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loki44
non serviam



Registered: 08/01/13
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Re: inoculating rye grain with liquid culture syringe... [Re: SupaThaRipper]
#19262407 - 12/11/13 07:59 PM (10 years, 1 month ago) |
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best way to prep LC
http://www.shroomery.org/forums/showflat.php/Number/6817701/page/2/fpart/1
just shoot into the jar, if LC is good mycelium will catch fire with all that food ready to be taken
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Sgt. Pepper



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Re: inoculating rye grain with liquid culture syringe... [Re: SupaThaRipper]
#19262414 - 12/11/13 08:00 PM (10 years, 1 month ago) |
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The beauty of liquid culture is that you can be a little wasteful with it since you've got so much. I've sprayed 5cc's all over a jar and shaken it and it colonized in less than a week. You want to shake after a lc inoculation since the spores have already germinated into mycelium and they don't have to search for a compatible match like with spore syringes. Also always always always check for contams!
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gratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
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Re: inoculating rye grain with liquid culture syringe... [Re: cronicr]
#19262417 - 12/11/13 08:01 PM (10 years, 1 month ago) |
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Quote:
SupaThaRipper said: Just stick the needle in the jar and aim for one of the sides. Shoot a little in... like 1ml or so. With lc id do just a couple jars at first to make sure iy isnt contaminated
so opposed to hitting all four holes with .5 ml a piece, you suggest just a single ml in one port? so i shouldnt tip the jar, just point the needle at the glass and blast a ml?
Quote:
cronicr said: heat-squirt-shake at 30
shake at 30, 30 degree angle, or 30 secs?
-------------------- Everybody got their own trip as you walk down the strip Your life could be changed by a talk with a stranger Someone told me a story they said that he knew me He told me a rhyme that transcended all time How we shared the same space, another time, another place He spoke with a rush about how so many lives have been touched
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262421 - 12/11/13 08:02 PM (10 years, 1 month ago) |
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lol sorry , heat your needle inoculate and shake at 30 %
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262431 - 12/11/13 08:03 PM (10 years, 1 month ago) |
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Just look up a TEK on how to inoculate with a syringe.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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gratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262442 - 12/11/13 08:04 PM (10 years, 1 month ago) |
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follow up question, im a lil worried I let my grain sit in the PC to long... is that possible? I did them pretty late last night, they were finished around 4 15ish in the am, thats when i flipped the valve open and turned off the PC. Its currently 7:05 pm... so they have been sitting a lil over 15 hours, are these guys still good to go?
-------------------- Everybody got their own trip as you walk down the strip Your life could be changed by a talk with a stranger Someone told me a story they said that he knew me He told me a rhyme that transcended all time How we shared the same space, another time, another place He spoke with a rush about how so many lives have been touched
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262444 - 12/11/13 08:05 PM (10 years, 1 month ago) |
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of course
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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gratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
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Re: inoculating rye grain with liquid culture syringe... [Re: 36fuckin5]
#19262450 - 12/11/13 08:06 PM (10 years, 1 month ago) |
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Quote:
36fuckin5 said: Just look up a TEK on how to inoculate with a syringe.
tried and failed, do you know of a thread that addresses this directly?
-------------------- Everybody got their own trip as you walk down the strip Your life could be changed by a talk with a stranger Someone told me a story they said that he knew me He told me a rhyme that transcended all time How we shared the same space, another time, another place He spoke with a rush about how so many lives have been touched
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gratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262515 - 12/11/13 08:17 PM (10 years, 1 month ago) |
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sorry just saw someone already posted a link... thanks for the help you guys are once again awesome
-------------------- Everybody got their own trip as you walk down the strip Your life could be changed by a talk with a stranger Someone told me a story they said that he knew me He told me a rhyme that transcended all time How we shared the same space, another time, another place He spoke with a rush about how so many lives have been touched
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: inoculating rye grain with liquid culture syringe... [Re: gratefulcheese]
#19262534 - 12/11/13 08:21 PM (10 years, 1 month ago) |
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If you flipped the valve as soon as you cut the heat, you just fucked your PC (which must be an expensive AA) and compromised your jars.
Don't buy a bunch of expensive stuff you don't know how to use, or at least read the manual.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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mrdelimeatslicer
Stranger
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Re: inoculating rye grain with liquid culture syringe... [Re: loki44]
#26052864 - 06/14/19 06:57 PM (4 years, 7 months ago) |
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Awesome
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Nichrome
I'm a torso!



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Re: inoculating rye grain with liquid culture syringe... [Re: cronicr]
#26052913 - 06/14/19 07:24 PM (4 years, 7 months ago) |
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Quote:
cronicr said: lol sorry , heat your needle inoculate and shake at 30 %
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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