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ubhuti
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Registered: 12/11/13
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Last seen: 9 years, 11 months
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Slow Start To Colonization
#19261937 - 12/11/13 06:46 PM (10 years, 1 month ago) |
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Semi-noob here. I had a lot of success a few years ago with WBS and casings thanks to you guys. I wanted to start another little project and have been disappointed with the results so far.
I soaked birdseed from the pet aisle (not WBS) for 24 hours, removed floaters, gave it many washes, dried it out a bit, and loaded into jars. The birdseed was slightly wet to the touch before loading, not dripping, so I feel I had the right balance there.
PC'd for an hour, waited till the next day to inoculate (1-3cc per pint jar), and placed in environment that stays between 75-85 degrees.
It has been 4-6 days (depending on the jar) and and I see next to no growth.
I have two questions:
1. Does regular pet bird seed have fungicide that I didn't know about? 2. Can I place the jars in 85-90 degree environment to stimulate even further growth?
I know I should be patient but I feel I have the jars in an ideal environment and should see some signs of growth at this point.
Thanks for the advice.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Slow Start To Colonization [Re: ubhuti]
#19261951 - 12/11/13 06:49 PM (10 years, 1 month ago) |
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those temps are too high and they should feel dry to the touch before the pc cycle, is there visible moisture in the jars? anywho 6 days is fuck all it can take up to and beyond a couple weeks to see growth
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ubhuti
Stranger


Registered: 12/11/13
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Re: Slow Start To Colonization [Re: cronicr]
#19262137 - 12/11/13 07:21 PM (10 years, 1 month ago) |
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There is very little noticeable condensation, but there is a bit visible. I have been reading threads of people's jars taking 2 weeks to colonize so I started getting worried. I also don't remember it taking this long the last time I tried my hand at it.
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MachineElf1.618
4-PO-Dimethyltryptamine



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Re: Slow Start To Colonization [Re: ubhuti]
#19264104 - 12/12/13 02:20 AM (10 years, 1 month ago) |
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its only been 4-6 days? Dude don't get paranoid just yet, your spores are probably just starting to germinate and you might be getting hyphal growth already. Give it another week and if you don't see anything than u can start getting worried. Don't put them in 85-90F the only growth you'll be promoting is the kind you dont want and don't shake the jars. Pretty much don't fuck with them just leave em alone and forget about them for a week. Be patient man.
Edited by MachineElf1.618 (12/12/13 02:24 AM)
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soicyboy97
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Registered: 12/28/10
Posts: 59
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Re: Slow Start To Colonization *DELETED* [Re: ubhuti]
#19264116 - 12/12/13 02:26 AM (10 years, 1 month ago) |
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Post deleted by soicyboy97Reason for deletion: x
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ubhuti
Stranger


Registered: 12/11/13
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Re: Slow Start To Colonization [Re: soicyboy97]
#19267673 - 12/12/13 08:00 PM (10 years, 1 month ago) |
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Thanks guys. I see a bit more growth today and will hold off on checking daily.
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Slow Start To Colonization [Re: ubhuti]
#19267685 - 12/12/13 08:02 PM (10 years, 1 month ago) |
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just put them on a shelf somwhere, you can check your grain jars whenever you want
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ubhuti
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Re: Slow Start To Colonization [Re: cronicr]
#19317976 - 12/23/13 07:30 PM (10 years, 1 month ago) |
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Been just over 2 weeks - now my only concern is the fact that I am using micropore tape for air exchange.
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PussyFart
Retired Cultivation Extrodinaire



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Re: Slow Start To Colonization [Re: ubhuti]
#19317994 - 12/23/13 07:34 PM (10 years, 1 month ago) |
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Quote:
ubhuti said: Been just over 2 weeks - now my only concern is the fact that I am using micropore tape for air exchange.

My concern is how are you gonna shake those jars? Looks like you left no room for them to move.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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twistedty
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Re: Slow Start To Colonization [Re: ubhuti]
#19317997 - 12/23/13 07:34 PM (10 years, 1 month ago) |
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you filled those way to full, and they need a shake but i dunno how youre going to do this
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twistedty
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Re: Slow Start To Colonization [Re: twistedty]
#19317998 - 12/23/13 07:35 PM (10 years, 1 month ago) |
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fuckin hacker
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ubhuti
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Re: Slow Start To Colonization [Re: twistedty]
#19318071 - 12/23/13 07:48 PM (10 years, 1 month ago) |
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Haha, whoops - didn't think about that. Do I really need to shake the jars? Does shaking promote air exchange? I guess it must.
Is this going to be a major problem?
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cronicr



Registered: 08/07/11
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Re: Slow Start To Colonization [Re: ubhuti]
#19318126 - 12/23/13 08:00 PM (10 years, 1 month ago) |
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It will test your patients
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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twistedty
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Re: Slow Start To Colonization [Re: ubhuti]
#19318996 - 12/23/13 11:10 PM (10 years, 1 month ago) |
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Quote:
ubhuti said: Haha, whoops - didn't think about that. Do I really need to shake the jars? Does shaking promote air exchange? I guess it must.
Is this going to be a major problem?
no it spreads the myc to all the grains, and ya its a problem
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ubhuti
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Re: Slow Start To Colonization [Re: twistedty]
#19322725 - 12/24/13 07:27 PM (10 years, 1 month ago) |
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Anything I can do to correct?
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Slow Start To Colonization [Re: ubhuti]
#19322745 - 12/24/13 07:31 PM (10 years, 1 month ago) |
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i can't think of any smart approach to it other then try your best
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blackglass6219
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Re: Slow Start To Colonization [Re: cronicr]
#19322936 - 12/24/13 07:55 PM (10 years, 1 month ago) |
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sterilize a few more jars, and pour excess into the empty jars? higher chance of contam but you should be all set in a SAB or in front of a flow hood
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ubhuti
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Registered: 12/11/13
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Thanks for the feedback guys. I figured I'd just play it as it lies.
So I am officially at the 3 week mark.
I am thinking of just casing what has colonized at this point, but I definitely appreciate your input. I am just concerned about the very little FAE and of course jars are too full.
Strains are: Bottom Left - KS Top Left - OI Second Column from Left: GT Third from Left: OI Far Right Column - B+
pics
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PussyFart
Retired Cultivation Extrodinaire



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Re: Slow Start To Colonization [Re: ubhuti]
#19340719 - 12/29/13 09:02 AM (10 years, 1 month ago) |
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Let it fully colonize....it will be another week....then spawn it to a bulk substrate, do not just case it....You will get more for your effort.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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blojo02184
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Registered: 05/15/13
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Re: Slow Start To Colonization [Re: PussyFart]
#19341312 - 12/29/13 12:27 PM (10 years, 1 month ago) |
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Have fun breaking them fuckers up...
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ubhuti
Stranger


Registered: 12/11/13
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Last seen: 9 years, 11 months
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Re: Slow Start To Colonization [Re: blojo02184]
#19342055 - 12/29/13 03:16 PM (10 years, 1 month ago) |
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I'm sure I could get a lot more out of these, but I'm not going to spawn to bulk for a number of reasons - thanks for the input though Nota.
Given that I am just going to straight case, can I case given these jars are not pure white mycelium? Is it ok that the bird seed is still quite visible?
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blojo02184
Big Red



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Re: Slow Start To Colonization [Re: ubhuti]
#19342117 - 12/29/13 03:26 PM (10 years, 1 month ago) |
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Well, my 2 cents, possibly dunk and tray them. After full colonization. The wide mouth jars shouldn't be too hard, but the shouldered jar is going to be a bitch.
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YOmamaPr0
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Registered: 12/19/13
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Re: Slow Start To Colonization [Re: ubhuti]
#19343269 - 12/29/13 08:04 PM (10 years, 1 month ago) |
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They look great except for the whole too full part, lol. Could you possibly pour some of the grain out in a SAB? Idk. However I'm sure you'll find a way of working it out. Good luck and keep updating!
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ubhuti
Stranger


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Re: Slow Start To Colonization [Re: YOmamaPr0]
#19373448 - 01/05/14 03:17 PM (10 years, 25 days ago) |
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I went ahead and cased all the colonized substrate and ended up with a lot more than I expected. After casing I placed them back at 80 degrees for further colonization - it has been a week but none of the mycelium has peaked through the casing layer yet. Should I just place them in the FC at this point or keep incubating until 20% mycelium covers the casing layer?
Also, in looking at the casings I noticed a few yellow droplets in the GT tub and was wondering if you guys knew what this is?
pic
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Slow Start To Colonization [Re: ubhuti]
#19373511 - 01/05/14 03:35 PM (10 years, 25 days ago) |
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metabolites and wait till 20 percent
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ubhuti
Stranger


Registered: 12/11/13
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Re: Slow Start To Colonization [Re: cronicr]
#19373517 - 01/05/14 03:37 PM (10 years, 25 days ago) |
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Thanks cronicr!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Slow Start To Colonization [Re: ubhuti]
#19373525 - 01/05/14 03:40 PM (10 years, 25 days ago) |
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keep any eye on the one showing metabs
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ubhuti
Stranger



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Re: Slow Start To Colonization [Re: cronicr]
#19373537 - 01/05/14 03:42 PM (10 years, 25 days ago) |
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do i need to be concerned about the metabolites? i read they are a sign of stress as well as normal excretion of waste? which is right?
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Slow Start To Colonization [Re: ubhuti]
#19373543 - 01/05/14 03:44 PM (10 years, 25 days ago) |
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both also may be a sign there fighting a contam, over colonization, heat stress lots of reasons so just watch it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ubhuti
Stranger



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Re: Slow Start To Colonization [Re: cronicr]
#19373801 - 01/05/14 04:46 PM (10 years, 25 days ago) |
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Got it. Many thanks for the help and advice.
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ubhuti
Stranger



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Re: Slow Start To Colonization [Re: ubhuti]
#19400955 - 01/10/14 08:12 PM (10 years, 20 days ago) |
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So it has been 12 days since casing. The first pic shows metabolites in the GT casing I was concerned about, and I now seen signs of them in the second pic of OI.
After 12 days of incubation after casing, there is still next to no mycelium showing in the casing layer of all my casings. Is it ok to continue to incubate these? Should I just place in the FC at this point?
Also, that extra colonized OI was placed in a zip lock (last pic) - thinking about trying an outdoor mini-grow with it. What do you guys think?
Thanks for the help and guidance fellas.
pics
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Slow Start To Colonization [Re: ubhuti]
#19400966 - 01/10/14 08:13 PM (10 years, 20 days ago) |
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i think you may have some bacteria
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ubhuti
Stranger



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Re: Slow Start To Colonization [Re: cronicr]
#19402483 - 01/11/14 05:48 AM (10 years, 20 days ago) |
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That's a bummer - what do you recommend I do with these cronicr?
Should I fruit the rest at this point?
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ubhuti
Stranger



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Re: Slow Start To Colonization [Re: ubhuti]
#19470969 - 01/25/14 08:09 AM (10 years, 6 days ago) |
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I went ahead and cased that GT with the metabolites showing.
cronicr, you think these will be ok?
pics
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Slow Start To Colonization [Re: ubhuti]
#19471367 - 01/25/14 10:31 AM (10 years, 6 days ago) |
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looks kind off but i may be wrong, you should get a good flush out of it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ubhuti
Stranger



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Re: Slow Start To Colonization [Re: cronicr]
#19492919 - 01/29/14 06:18 PM (10 years, 1 day ago) |
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wanted to provide an update on the GT.
there are also 2 pics of KS.
the OI has not yet started pinning, even though it was the most aggressive colonizer.
Everything look ok so far guys?
pics
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