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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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A specific and general question on shaking g2g transfers.
#19260501 - 12/11/13 02:28 PM (10 years, 1 month ago) |
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I've got two semi-related questions on g2g transfers. The first is just a general preference, and that is in your opinion do you find it beneficial to shake g2g transfers after a period of colonization, or do you just do the one initial shake and leave it be? My reason for asking leads me to the more specific question.
Normally I do just one shake, the initial one. My thought is, well, I don't see a second shake slowing it down or speeding it up, although I've never tried so I can't say for sure. However right now I have a few jars which colonized pretty quickly, about 6 or 7 days, however there are some stray grains stuck to the glass higher up that did not get colonized. I can't knock them off either. Shaking would obviously take care of them, but it just seems wasteful to me in that it would take unnecessary energy out of the mycilium just to colonize literally 4 or 5 grains. Would these few grains be a big enough contamination vector when spawning or am I worrying over nothing? I doubt I can knock them out after opening it since I can't knock them off right now so my choices are spawn as is, shake, or I thought of maybe just rinsing them out with water before breaking the jars up and spawning. It's kind of trivial I know, but I just wanted to get some opinions, I don't want to spawn them as is, which I'm thinking of doing, if it'll lead to contamination that could have easily been prevented.
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TheCyndicate
Conglomerate



Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 8 months
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Re: A specific and general question on shaking g2g transfers. [Re: krypto2000]
#19260545 - 12/11/13 02:33 PM (10 years, 1 month ago) |
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Shake about 30% it defently helps speed things up . Also it the jar does not recover after the shake its most likely contaminated
Cyn
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: A specific and general question on shaking g2g transfers. [Re: TheCyndicate]
#19260550 - 12/11/13 02:34 PM (10 years, 1 month ago) |
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i dont shake right as i g2g, i wait for some growth first so any contams can be spotted
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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twistedty
Forcefully Retired



Registered: 07/01/12
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Re: A specific and general question on shaking g2g transfers. [Re: TheCyndicate]
#19260562 - 12/11/13 02:35 PM (10 years, 1 month ago) |
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the seeds at the top are fine bro. a few uncolonized grains arent gonna crush your grow bro.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: A specific and general question on shaking g2g transfers. [Re: twistedty]
#19260600 - 12/11/13 02:41 PM (10 years, 1 month ago) |
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I shake mine once at inoculation, then one more time at about 30%. I stretch my masters to about 25-30 jars though.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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krypto2000
Unknown


Registered: 12/05/06
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Last seen: 4 years, 3 months
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Re: A specific and general question on shaking g2g transfers. [Re: 36fuckin5]
#19262371 - 12/11/13 07:54 PM (10 years, 1 month ago) |
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Quote:
36fuckin5 said: I shake mine once at inoculation, then one more time at about 30%. I stretch my masters to about 25-30 jars though.
By inoculation do you mean right after the g2g transfer? What do you mean by 25-30 jars? You mean you turn 1 jar into 25-30? I normally turn 1 jar into 7, and then those 7 I turn into 49 and then use all of those to spawn. Basically I just follow a rule of two transfers and typically do 1:7 ratio with each transfer, though sometimes up to 10, just depends what I have on hand, but 7 tends to be the most efficient based on the amount of jars I have as well as the rotation/schedule.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: A specific and general question on shaking g2g transfers. [Re: krypto2000]
#19262388 - 12/11/13 07:56 PM (10 years, 1 month ago) |
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Yes, a G2G transfer is a form of inoculation.
I mean I take one master jar and transfer to up to 25 or 30 jars. Always at least 12-15.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: A specific and general question on shaking g2g transfers. [Re: 36fuckin5]
#19262469 - 12/11/13 08:10 PM (10 years, 1 month ago) |
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With G2G, I shake them right after and that's all they usually need.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: A specific and general question on shaking g2g transfers. [Re: SpitballJedi]
#19262549 - 12/11/13 08:24 PM (10 years, 1 month ago) |
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You probably also do the standard 10:1 transfer rate. I do more than twice that.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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twistedty
Forcefully Retired



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Posts: 5,487
Loc: Middle
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Re: A specific and general question on shaking g2g transfers. [Re: SpitballJedi]
#19263635 - 12/11/13 11:51 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: With G2G, I shake them right after and that's all they usually need.
depends on the ratio from the master. i usually do 10 per master. but in theory a few grains would do a whole jar.
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TheApprentice
back at it



Registered: 09/25/11
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Re: A specific and general question on shaking g2g transfers. [Re: twistedty]
#19263749 - 12/12/13 12:13 AM (10 years, 1 month ago) |
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I make baby masters in 1/2 pt jars for my agar transfers. I only put enough grain in to cover the bottom or so. Then use that to inoculate 2 quart jars. It goes so much quicker ime... Cuz once u can do a g2g, you can easily end up with more spawn then u know what to do with...
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RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: A specific and general question on shaking g2g transfers. [Re: twistedty]
#19264688 - 12/12/13 08:05 AM (10 years, 1 month ago) |
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I usually do 1:7 because my PC holds 7. I just recently got a bigger PC so I'll probably start doing 1:10.
Yeah, the farther you spread the master, the more likely you may want to shake around 2%-30%.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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twistedty
Forcefully Retired



Registered: 07/01/12
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Re: A specific and general question on shaking g2g transfers. [Re: SpitballJedi]
#19265792 - 12/12/13 01:20 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: I usually do 1:7 because my PC holds 7. I just recently got a bigger PC so I'll probably start doing 1:10.
Yeah, the farther you spread the master, the more likely you may want to shake around 2%-30%.
mine holds 7 with 3 on top so i do 10, guess it all boils down to your equip etc also
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TheApprentice
back at it



Registered: 09/25/11
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Re: A specific and general question on shaking g2g transfers. [Re: twistedty]
#19268671 - 12/12/13 11:32 PM (10 years, 1 month ago) |
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I do 7 in my PC. I only have about 20 jars colonizing from MS right now 
and my current SAB only holds 7 jars comfortably. I always get weird about using a master, then stopping to reload the GB. I need a bigger sab so I can g2g like 14 at a time...
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RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: A specific and general question on shaking g2g transfers. [Re: TheApprentice]
#19270469 - 12/13/13 12:04 PM (10 years, 1 month ago) |
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Like this 

My pc holds 10, but I still do 1:7 for some reason.
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sharpshroomer98
Student of the Universe.



Registered: 08/30/11
Posts: 252
Last seen: 11 days, 18 hours
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Re: A specific and general question on shaking g2g transfers. [Re: krypto2000]
#19270698 - 12/13/13 01:10 PM (10 years, 1 month ago) |
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Quote:
krypto2000 said: Like this 

My pc holds 10, but I still do 1:7 for some reason.
Krypto, do I see a fellow guitar player in these forums!? Sorry for off topic just like to see a fellow musician haha
-------------------- No, I wasn’t born in 98’
 
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: A specific and general question on shaking g2g transfers. [Re: sharpshroomer98]
#19270922 - 12/13/13 02:04 PM (10 years, 1 month ago) |
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I wish . My ex was supposed to be teaching me to play, but she stopped hanging out with me more or less out of the blue after only a couple of lessons and I haven't tried on my own since. Might pick it up sometime, but I would always get very frustrated practicing myself, it's sooo difficult in the beginning just to play basic chords, my hands do not enjoy it.
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