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Offlinetwistedty
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19255499 - 12/10/13 01:44 PM (10 years, 2 months ago)

Quote:

Camtaro420 said:
The temp has been staying at 90 mostly I do my best to get it between 80-90 but when it's cold the heater is on harder and it's by the heater in a closet so it's as good as it gets/ We left the heater off for just a few hours and everything dropped to 60.

It's never gone past 90, or below 60.

And I innoced 2.5 days ago.





thats way to hot, aim for 75.  did you use a spore solution?


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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: twistedty]
    #19255513 - 12/10/13 01:49 PM (10 years, 2 months ago)

I'm trying to keep it cooler yeah.

and I used an old spore syringe bought of some website some time ago. IT was given to me by a friend it was kept dark in a zip lock plastic bag in postage box, in a fridge for about 6 months.


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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19255707 - 12/10/13 02:36 PM (10 years, 2 months ago)

I'm hoping that Jar A & B just have some weird jelly stuff happening. But is it possible it's



Bacteria like this picture?

It seems clear when you look at it through the side of the glass.


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InvisibleJ. Jack Flash
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19257111 - 12/10/13 07:12 PM (10 years, 2 months ago)

Quote:

Camtaro420 said:
I'm hoping that Jar A & B just have some weird jelly stuff happening. But is it possible it's



Bacteria like this picture?

It seems clear when you look at it through the side of the glass.



no idea but that looks freekin cool.  considering its shape, it's like it's shriveling up and pulling away from the sides of the container.  bacteria does that?  is it just drying out?  what's it look like lit from behind?  how's it taste?  (don't taste it  :eek:)


--------------------
 
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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: J. Jack Flash]
    #19257734 - 12/10/13 09:24 PM (10 years, 2 months ago)

It looks more like drying of the agar then the bacteria in the contaminate example picture.


On another note, I just realized an hour ago that I used 3 times the honey then I was suppose to.....

How will this effect things?


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Offlinenewera
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19257743 - 12/10/13 09:25 PM (10 years, 2 months ago)

isn't honey anti-fungal?


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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: newera]
    #19257854 - 12/10/13 09:46 PM (10 years, 2 months ago)

Right that's why you are suppose to use very little. Should I try to retrieve the spores and cultivate them on a clean properly made agar, like you would an isolate?


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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19260065 - 12/11/13 01:00 PM (10 years, 2 months ago)

OK, so time for an update.

Things to know:

- I messed up the Grocery Store Agar Tek. My flakes should have been grinded instead of forked for smoother consistency, even more so for my agar agar flakes and additionally I used 2 teaspoons of honey more then I was suppose to. Because of this the Agar my spores are sitting in, is more like a more liquid like substance that has separated from the solids in the lower half of the jars.

- The spores are old. While I believe they were taken care of, it doesn't change the fact it was an old spore syringe, and the older they are the more likely it is they were contaminated with bacteria. However I used all the common sterile procedures.

- Sterile procedures: I used Alcohol mist to pull contaminates down to the bottom of the glove box each time before entering with my hands, flame hot syringe before each injection, tapes over open holes, band aids to prevent extra airflow of the petris, etc. I DID use my bare hands though.. The gloves I got wouldn't work well so I would just douse my hands with alchohol each time I took the syringe out to flame sterilize so they were dripping with alcohol at any given time.

- The temperature & light: The temperature has been all over the place. It started at 90 mostly, it's dipped down to 60 on a day we didn't notice and for the past day or so it's at about 80. Lighting is at least 5-8 hours of continual dark, during the day time it gets ambient light no direct light unless you count my phone light for during inspection.

- Self-Healing Lids: This was completely unnecessary for petris. Someone told me this, but I figured WTF it's a good way to test em out, and it was. I can see that I didn't have the silicone cured enough for one. Should have waited 3 days as opposed to 1. There needs to be silicone around any sort of edge that was exposed to moisture. I can see that all of the microfiber tape has rust building up making it a no longer suitable filter and possibly will soon start to drip rust down into the petri. Long story short, DON'T BOTHER USING THESE LIDS FOR ANYTHING OTHER THEN A SUBSTRATE FILTER FOR THINGS LIKE BRF CAKES. 

So that's the jist. It's been 3.5 days, and I have yet to see anything promising. I know patience is key but I have a strong feeling I f*cked up. Just sort of rushed into things in excitement. That's ok though, learning from mistakes is the best way sometime.

I made new petris that look perfect. I also ordered some new spores from hakeye (they are supposedly albino's, that would be pretty sweet if it comes through as advertised.) I am ready to try again if this experiment fails.

With that I ask, where should I go from here? Should I just give these faux petris a chance at germination still? Should I try and retrieve the clumps of spores I can see sitting in the faux agar and transfer the, to proper agar? Lay it on me brothers.

Here's some glorious pictures of my failures.

Jar 1 (The weirdest looking one)



Jar 2



Jar 3 ( I am such a fool of a took!)



Jar 4 (Least of the weird looking ones)



Jar 5 (Just weird)



I'm guessing I just have to wait for some new spores at this point, but if any of you think some spores are salvageable, I am willing to try to isolate them. On the least weird jar I can still actually see some clumps of spores where they were squirted into the jar.


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InvisiblebodhisattaMDiscordReddit
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19260121 - 12/11/13 01:14 PM (10 years, 2 months ago)

miracle grow perlite works just fine, it's getting rinsed off with water anyway and never touches the cakes. It's all I have ever used without any problem ever.

I would ditch the lysol and peroxide.
I would make agar with potato dextrose or malt extract
I would pour petri dishes rather than fuck around with jars
Order some cheap dishes since you obviously have money to spare
500ml h2o + 10g agar + 10g malt extract, PC for 30M in a 20$ pressure cooker you can find almost anywhere especially thrift stores and craigslist. pour agar inside of a SAB(not glove box)
win.

you could have spent 30$ and been started on PF cakes


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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: bodhisatta]
    #19260155 - 12/11/13 01:22 PM (10 years, 2 months ago)

Quote:

bodhisatta said:
miracle grow perlite works just fine, it's getting rinsed off with water anyway and never touches the cakes. It's all I have ever used without any problem ever.

I would ditch the lysol and peroxide.
I would make agar with potato dextrose or malt extract
I would pour petri dishes rather than fuck around with jars
Order some cheap dishes since you obviously have money to spare
500ml h2o + 10g agar + 10g malt extract, PC for 30M in a 20$ pressure cooker you can find almost anywhere especially thrift stores and craigslist. pour agar inside of a SAB(not glove box)
win.

you could have spent 30$ and been started on PF cakes




So you pour sterilized agar into the dishes, what do you do for sterilization of the dishes themselves? Should I get glass or plastic ones? Also what kind of tape is it that I see around them in most of the pictures around here? and could you please recommend a good still air box thread? I have yet to find one, but I haven't really looked that hard.


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Offlinetwistedty
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19260219 - 12/11/13 01:38 PM (10 years, 2 months ago)

you can buy pre-sterilized ones, good for one use.  i got a case of 500 for around 50-75bux.  they come in 25 sleeves, 25 per sleeve.

a SAB is nothing more than a big square sterilite container with 2 holes for your arms. 


http://www.mycosupply.com/ this is a good place to start get some parafilm, sfds, and pda if you have money.


http://www.ebay.com/itm/PARTIAL-CASE-OF-340-BD-FALCON-OPTILUX-STERILE-PETRI-DISHES-351001-/360795184288?pt=LH_DefaultDomain_0&hash=item5401119ca0

i didnt check the dimensions of the dishes but this will give you an idea


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Offlinetwistedty
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: twistedty]
    #19260221 - 12/11/13 01:38 PM (10 years, 2 months ago)

just depends on how seriously you want to take this hobby and the right tools helped me alot


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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: twistedty]
    #19260358 - 12/11/13 02:04 PM (10 years, 2 months ago)

Thanks for thot. Got some petri's on the way right now. I have money to spend yeah, not THAT much though lol.

I also got some pre-made agar sterilized ones from a reputable ebay seller for the new spores I am getting. That way all I have to do is squirt some spore solution into premade refrigerated agar dishes and from that I can play lets isolate and grow awesome mushrooms.

The only thing now is what to do with the things sitting in my cupboard? just let them....... do whatever?


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InvisiblebodhisattaMDiscordReddit
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19260417 - 12/11/13 02:15 PM (10 years, 2 months ago)

pouring your own agar lets you see how clean you are too. No contams then you know you're pretty clean and can feel comfortable about working in a SAB to do G2G or agar inoculations with grains.

Like said above just buy them pre-sterilized (those are plastic and can't be reused)

I have glass dishes which can be re-used but are a little bit more of a pain in the ass to handle.

You can wrap your dishes with parafilm(the defacto standard) or saran wrap.

as for the sab

Quote:

Notahacker420 said:
If you are getting a 75% success rate on agar in a SAB, your sterile technique needs some fine tuning, not the SAB.

A flow hood is not going to fix this.

I get 99-100% success rate in my SAB, with no thought of building a flow hood anytime soon.

   




it's really just a big ass clear box with holes in it. Make it to fit your body, you don't need fancy clean outs for your holes but you do want to cover the holes for ~10m before doing work inside of your SAB to make the air settle(still air= no contams get into your work) you can clean your sab with soap and water you dont need to use alcohol or bleach at all since the still air is what you want. It's not a sterile air box but it allows you to work without getting contamination into your work. This is the second best tool with a laminar flow hood being the first(aside from an iso9000 level 5 clean room)

The lets grow mushrooms video series has an agar prep tek


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OfflineCamtaro420
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Re: Scratch Grow: Agar Tek, BRF Cake Tek, Glove Box Tek, RR Style [Re: Camtaro420]
    #19260513 - 12/11/13 02:30 PM (10 years, 2 months ago)

Nevermind about letting them be I decided to inspect them and good thing I did they all smell rotten northing worth transferring or anything for that matter. Just a learning experience in failure.


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OfflineCamtaro420
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Re: Jumping into the hobby to fast. [Re: Camtaro420]
    #19260731 - 12/11/13 03:07 PM (10 years, 2 months ago)

Ok so I went ahead and changed the title and added a pre-letter to the thread talking about how I rushed everything.

Quote:

Camtaro420 said:
Hello everyone, my name is Camtaro420.

This is a story about how I was a dumba$$ who rushed into things too quickly. while I used awesome teks, I did NOT follow them to the letter as many post's suggested that I do. Waiting, and patience is key. I lost 6 jars, a spore syringe, and about 4 days worth of time that could have been better spent doing it the right way. This is on top of the fact that all my supplies for growing could have been cheaper if I had researched a little harder and asked for advice before I started growing. This thread shall be the first and end of my failures. From here forth I will be taking the advice from the pro's. Thanks to everyone so far who has helped.




Everything else is left now for education to those who want to read. When I get my new syringe I will start a fresh thread. I also think I will be working in correspondence to blue's new thread which will also be using agar to start from scratch. Here's a picture of the still air box I built.



Hopefully it will help in the future.


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Re: Jumping into the hobby to fast. [Re: Camtaro420]
    #19260754 - 12/11/13 03:10 PM (10 years, 2 months ago)

:manofapproval:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Jumping into the hobby to fast. [Re: cronicr]
    #19260766 - 12/11/13 03:13 PM (10 years, 2 months ago)

:electrickoolaid:

Some people find it easier to take the lid off and use the SAB upside down IE the opening of the box is the floor. This way you can use a completely smooth flat surface such as a table(sanitize first) or a bleach soaked towel(if you want a less slippery surface).

Save the lid though I use my SAB as storage when I'm not using it.

I personally use a coffee table. I wipe it with alcohol put the SAB over it. I spray the inside of my SAB with a very very dilute hot water and dish soap mix(like a half a drop) then I plug the holes and let it sit for a good 10 minutes( with the things I want to work with already inside )

It's good practice to flame outside of the box rather than having a flame inside(it will stir up the air :thumbdown: )

It's also a good idea to do some practice runs without using a culture just to get familiar with how to "drive your SAB" I found out the SAB moved around so I put some bricks around my SAB so it stays put when my arms hit the side of the arm holes.



Edited by Trusted cuItivator (12/11/13 03:19 PM)


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OfflineCamtaro420
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Re: Jumping into the hobby to fast. [Re: bodhisatta]
    #19261008 - 12/11/13 03:57 PM (10 years, 2 months ago)

Quote:

bodhisatta said:

Some people find it easier to take the lid off and use the SAB upside down IE the opening of the box is the floor. This way you can use a completely smooth flat surface such as a table(sanitize first) or a bleach soaked towel(if you want a less slippery surface).

Save the lid though I use my SAB as storage when I'm not using it.

I personally use a coffee table. I wipe it with alcohol put the SAB over it. I spray the inside of my SAB with a very very dilute hot water and dish soap mix(like a half a drop) then I plug the holes and let it sit for a good 10 minutes( with the things I want to work with already inside )

It's good practice to flame outside of the box rather than having a flame inside(it will stir up the air :thumbdown: )

It's also a good idea to do some practice runs without using a culture just to get familiar with how to "drive your SAB" I found out the SAB moved around so I put some bricks around my SAB so it stays put when my arms hit the side of the arm holes.






These are all brilliant ideas. My mother in-law has some old spores in the fridge. Like really old ones from back in her grow days. I may even practice with those before using my good syringe incoming in a week.


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InvisiblebodhisattaMDiscordReddit
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Re: Jumping into the hobby to fast. [Re: Camtaro420]
    #19261018 - 12/11/13 03:59 PM (10 years, 2 months ago)

When I see a new person come to the shroomery and demonstrate they can learn from other peoples mistakes rather than feverishly needing to make their own I'll spend some time giving my tips.

You'll probably end up being the next decent/good cultivator on the forums if you keep this up.


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