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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 9 months
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How would grow mushrooms in a sterile environment?
#19260258 - 12/11/13 01:48 PM (10 years, 1 month ago) |
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pretty much as the title states, how would one grow mushrooms in a sterile environment to ensure clean spore printing, etc. Invirtro growing? maybe?
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: How would grow mushrooms in a sterile environment? [Re: fractaldill]
#19260294 - 12/11/13 01:54 PM (10 years, 1 month ago) |
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seriously?
polycarbonate culture bottles, BRF / fert agar, and you can swab out unlimited sterile spores in about 3 weeks.

doesnt get any faster, easier, or more sterile to my knowledge.
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Edited by anne halonium (12/11/13 02:07 PM)
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 9 months
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Re: How would grow mushrooms in a sterile environment? [Re: anne halonium]
#19260311 - 12/11/13 01:57 PM (10 years, 1 month ago) |
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can you elaborate more on the process please?
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: How would grow mushrooms in a sterile environment? [Re: fractaldill]
#19260369 - 12/11/13 02:06 PM (10 years, 1 month ago) |
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hmmm........
first ya would need some large culture bottles. mine are .5 liter polycarbs. you could substitute.
then youll need fert agar. reason why, it grows fast and dense, and fruits easy and quick.
sterilize a fert agar bottle in a PC cool on side as a slant.
aim at a light, we use blue 450 nm LED, and with lid slight cracked, bottle on side. nearly always they fruit, and some bottles will flush.
collect spores with long sterile swabs galore.

this is one of our fave ways to get the best , cleanest spores.
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Edited by anne halonium (12/11/13 02:08 PM)
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: How would grow mushrooms in a sterile environment? [Re: fractaldill]
#19260385 - 12/11/13 02:09 PM (10 years, 1 month ago) |
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Most people don't worry so much about it... Just be sterile in your spore printing procedures... Then go from the spore print to agar...
This way you can make sure it's clean before noc'ing up a bunch of shit 
Here's a good link to some basic, sterile spore printing procedures. Using a SAB or GB would be a great addition to this simple tek. You can use mason jars to cover them instead of petris if you don't have any around.
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 9 months
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Re: How would grow mushrooms in a sterile environment? [Re: PirateSwazey]
#19260407 - 12/11/13 02:13 PM (10 years, 1 month ago) |
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be careful with anne in here
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 9 months
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Re: How would grow mushrooms in a sterile environment? [Re: anne halonium]
#19260410 - 12/11/13 02:14 PM (10 years, 1 month ago) |
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Quote:
anne halonium said: hmmm........
first ya would need some large culture bottles. mine are .5 liter polycarbs. you could substitute.
then youll need fert agar. reason why, it grows fast and dense, and fruits easy and quick.
sterilize a fert agar bottle in a PC cool on side as a slant.
aim at a light, we use blue 450 nm LED, and with lid slight cracked, bottle on side. nearly always they fruit, and some bottles will flush.
collect spores with long sterile swabs galore.

this is one of our fave ways to get the best , cleanest spores.
Thanks, sounds like a very good method.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: How would grow mushrooms in a sterile environment? [Re: PirateSwazey]
#19260415 - 12/11/13 02:15 PM (10 years, 1 month ago) |
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this tek doesnt need alot of complextiy. make em and go, large sterile swabs at any drugstore.
swabs can be stored in sterile tubes.
essentially this method allows for you to see the entire process. use it when you need sterile spores assured.
simple, direct, scale-able.
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Edited by anne halonium (12/11/13 02:17 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: How would grow mushrooms in a sterile environment? [Re: fractaldill]
#19260418 - 12/11/13 02:16 PM (10 years, 1 month ago) |
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 colonized grain to a sterilized bulk sub may do it too
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: How would grow mushrooms in a sterile environment? [Re: cronicr]
#19260434 - 12/11/13 02:19 PM (10 years, 1 month ago) |
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I'd do an invitro bag on a sterile bulk sub if I were only aiming for prints.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How would grow mushrooms in a sterile environment? [Re: cronicr]
#19260458 - 12/11/13 02:22 PM (10 years, 1 month ago) |
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you can also sterilize swabs by putting them in a jar and PCing them.
violet has a good write up about using invitro to get clean prints
We shouldn't call it in-vitro with PP5 but I digress, there's no latin word for plastic, though it's just a mere colloquialism so any jar is really in-vitro even if its pp5.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: How would grow mushrooms in a sterile environment? [Re: 36fuckin5]
#19260475 - 12/11/13 02:26 PM (10 years, 1 month ago) |
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its possible, to get invitro fruits on sterile subs. thing is, occasionally they co opt with bacteria. looks good, then mysteriously contams.
fert agar, minimizes that. bacteria can be spotted, when it co opts a culture.
doubt me? im pretty sure the MC has micro pics, of bacteria co opting p cube mycelia with, like ,electron microscope.
although unseen, i suspect bacteria is the number one plague of spores.
avoid it.
excellent bod, violet does take a clean print indeed.
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Edited by anne halonium (12/11/13 02:26 PM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: How would grow mushrooms in a sterile environment? [Re: anne halonium]
#19260559 - 12/11/13 02:35 PM (10 years, 1 month ago) |
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It happens, but if your sterile procedure is good it shouldn't.
Personally, I just take a print like normal in my SAB and have no problems.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How would grow mushrooms in a sterile environment? [Re: 36fuckin5]
#19260588 - 12/11/13 02:39 PM (10 years, 1 month ago) |
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Quote:
36fuckin5 said: Personally, I just take a print like normal in my SAB and have no problems.

Prints should go to agar anyway, many people have success with wild prints all day long so a indoor print using clean technique is going to be A-OK
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: How would grow mushrooms in a sterile environment? [Re: 36fuckin5]
#19260606 - 12/11/13 02:42 PM (10 years, 1 month ago) |
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i can make a half liter of MS spore solution in seconds, with a high degree of confidence, and no waiting.

alot of stuff works good enough. some stuff is reliable, fast , and direct for scale.
when ya need precision sterile spores galore,ya know what to do
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: How would grow mushrooms in a sterile environment? [Re: anne halonium]
#19260628 - 12/11/13 02:46 PM (10 years, 1 month ago) |
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Well I don't see why I'd need mass amounts of spores when I use isolates. If I take spore prints, they're generally for long-term backup or trading.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Re: How would grow mushrooms in a sterile environment? [Re: 36fuckin5]
#19260634 - 12/11/13 02:46 PM (10 years, 1 month ago) |
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Quote:
36fuckin5 said: Well I don't see why I'd need mass amounts of spores when I use isolates. If I take spore prints, they're generally for long-term backup or trading.
selling spore syringes
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How would grow mushrooms in a sterile environment? [Re: anne halonium]
#19260637 - 12/11/13 02:47 PM (10 years, 1 month ago) |
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Quote:
anne halonium said: i can make a half liter of MS spore solution in seconds, with a high degree of confidence, and no waiting.

alot of stuff works good enough. some stuff is reliable, fast , and direct for scale.
when ya need precision sterile spores galore,ya know what to do
I don't like most of us, I'm growing a few eigths a week for my own enjoyment, and occasionally my wife too.
Just like with weed. There's no knocking the people doing 2-3 plants with a couple hundred bucks invested, but if you're going to do it right something resembling a lab is the way to go.
Same with the differences between a 5 gal homebrewer and a craft microbrewery. There's some similarities and some huge differences. Neither are right or wrong. There's all different sized cultivators here. Pick what you like and stick with what works for you.
it's like taking advice from amazon to run a garage sale.
Edited by Trusted cuItivator (12/11/13 02:48 PM)
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: How would grow mushrooms in a sterile environment? [Re: bodhisatta]
#19260673 - 12/11/13 02:53 PM (10 years, 1 month ago) |
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amazon/ amazin same diff, THX bod.
see , sometimes i wanna burn thru 100 or so pp5's to look for clones. and i dont want alot of drama doing it.....
hence , the half liter insta MS.
maybe knock back a few dozen polycarbs for next round.

slants, do racks full in minits.

even works for plates.
big or small, culture bottles enhance us all. only downside? cant buy em at the mall.
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Edited by anne halonium (12/11/13 02:58 PM)
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Re: How would grow mushrooms in a sterile environment? [Re: anne halonium]
#19261647 - 12/11/13 05:44 PM (10 years, 1 month ago) |
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Quote:
anne halonium said: amazon/ amazin same diff, THX bod.
see , sometimes i wanna burn thru 100 or so pp5's to look for clones. and i dont want alot of drama doing it.....
hence , the half liter insta MS.
maybe knock back a few dozen polycarbs for next round.

slants, do racks full in minits.

even works for plates.
big or small, culture bottles enhance us all. only downside? cant buy em at the mall.
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