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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
    #19291189 - 12/17/13 11:59 PM (10 years, 1 month ago)

2nd xfer from pe mother (no-pour) dish.  I would like one more clean transfer and a reduction in genetics.  This jar used a very nutritious agar, and after it enveloped the surface, began to make pins.  None got bigger than that, but 8 were xfered this evening.  Using agave nectar and karo agar recipes in hopes getting this process moving.


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19292124 - 12/18/13 07:34 AM (10 years, 1 month ago)

after not being home for 5 days i came home to all my dishes fully contaminated :frown: so i'm gonna noc some more up today and hopefully be able to be more diligent about my sterile technique and watching for the nasties...


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19292734 - 12/18/13 10:36 AM (10 years, 1 month ago)

Quote:

blueconfusion said:
after not being home for 5 days i came home to all my dishes fully contaminated



No-pour?  I do no-pour and found that 20 minutes in the cooker is simply not enough.  45 minutes works 100%.  I am not certain your method however.


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19292785 - 12/18/13 10:52 AM (10 years, 1 month ago)

No i do a pour in a SAB i was thinking about switching to no-pour but i have a bunch of dishes already poured so i just used those.  i do my agar in the pc for at least 40 min.  My failure with my first inoculation was my sterile technique coupled with wild spores and not being home for almost a week to catch the bacteria that had moved in.  i have had no problems with clones, grains or transfers on agar before i just was a little distracted and not home, but as soon as i run out of dishes i have the pp5 ziploc half pint jars already so i'm definitely gonna try it at some point.  i'm glad you're here whippy you seem to be very knowledgeable at this hobby called mycology!:thumbup:


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19292790 - 12/18/13 10:53 AM (10 years, 1 month ago)

Quote:

blueconfusion said:
  i'm glad you're here whippy you seem to be very knowledgeable at this hobby called mycology!:thumbup:



u should see his math skills lol


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19292822 - 12/18/13 11:04 AM (10 years, 1 month ago)

Quote:

Whippy said:
2nd xfer from pe mother (no-pour) dish.  I would like one more clean transfer and a reduction in genetics.  This jar used a very nutritious agar, and after it enveloped the surface, began to make pins.  None got bigger than that, but 8 were xfered this evening.  Using agave nectar and karo agar recipes in hopes getting this process moving.




this is my 2nd PE transfer onto PDYA from a leftover grain dropped doing g2g in my SAB that grain master had a full canopy and beautiful fruits!


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
    #19292928 - 12/18/13 11:37 AM (10 years, 1 month ago)

I had initially cultured PE from a swab to a pair of nute heavy dishes.  The slow growth from the nutes and the apparent overage of spores created something that I simply did not believe was even a mushroom.  The swab was tossed out right after, and one of the jars was retired for being too old. 

I moved a number of what looked like pins forming to a few faster growing media.  I hope to get some good rizo growth, get a small spattering of genes and send it off to the grain factory.



Quote:

blueconfusion said:
this is my 2nd PE transfer onto PDYA from a leftover grain dropped doing g2g in my SAB that grain master had a full canopy and beautiful fruits!



That bugger has growth all over.  Its bonkers how one side is so hungry, and the other is simply content to sit and digest.  I grew out a grain one time.  It was a great time, and I figured that a millet seed being so small shouldn't have too many genetics.....woah, I think it had all of them.  I think I ended up keeping track of about 30 jars, differing poor-man-agar recipes, and never fruited a single one.  I stumbled upon a very large fruit that needed cloned, and my studies took a different direction.  I learned quite a bit about nutrients and benefits on agar because I refuse to order supplies.  I have used quite a bit of at home stuff that has and will work well for different purposes.



Quote:

cronicr said:
u should see his math skills lol



231 cubic inches what?


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19292961 - 12/18/13 11:46 AM (10 years, 1 month ago)

i meant to get a clone off the tub i had done of PE, but i was harvesting right before and after work that weekend and never had a chance to keep up those 2 tubs came in at over 3/4 lbs. from a single grain transfer from an MS, first flush.  this time i only grew out one tub so hopefully i'll get a nice clone! 
i don't order any of my agar online either i like to have complete control of what goes in everything i do is local and made in my home


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19296851 - 12/19/13 07:12 AM (10 years, 1 month ago)

I re inculcated my petri dishes yesterday.  Hopefully I'll pay closer attention this time...
I also spawned some grain jars to straw hpoo coffee buffalo poo ewc and gypsum.


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19297101 - 12/19/13 08:48 AM (10 years, 1 month ago)

Sweet!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19297417 - 12/19/13 10:27 AM (10 years, 1 month ago)

I set up a race for 8 transferred cultures the night before last. 


All PE transfers are showing evidence of recovery.  I am very gentle with moving cultures around.  I am hoping they decide to go fast to the edges. 

I had also made a few xfers from a more recent MS Eq plate.  This was an indoor print I collected. 

I cut back nutes in the agar on a few to really get them jumping.


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OfflineStromriderM
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19310682 - 12/22/13 06:03 AM (10 years, 1 month ago)

Here is my wild Austrailian culture that I took to grain a few days ago


I been a busy beaver


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OfflineJeeter
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Stromrider]
    #19311859 - 12/22/13 01:13 PM (10 years, 1 month ago)

Nice bean shelf ya got there.


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Jeeter]
    #19312107 - 12/22/13 02:25 PM (10 years, 1 month ago)

:headbang3:
so far all i've got done is my cambo's and john allen strain on grain


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
    #19312653 - 12/22/13 04:52 PM (10 years, 1 month ago)

Just got back in town from a Xmas party and got germination on my wild Aussies, no visible contams :thumbup: Looks like my plate with the hericium coralloides is doing alright with no visible contams and that print was guaranteed filthy :lol: hopefully I see germination soon. So far the tetra agar is doing its job.


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InvisibleHypnotoadCroaked
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Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Stromrider]
    #19312869 - 12/22/13 05:47 PM (10 years, 1 month ago)

Quote:

Whippy said:
I set up a race for 8 transferred cultures the night before last. 




100% of Pe xfers failed.  Each picked up an unidentified mold.  I had to chuckle.  In 3 months of using the SAB, I have yet to pollute anything. 

2 Survivors (not PE) are alive and well.  Leads me to think the pe was a fail, and it is being relocated to the dead wood pile.

Quote:

Stromrider said:
I been a busy beaver




You seem to be in the same gear I am.  I ran out of lids today.  I have to run out and find some small mouth lids if I want to continue.  I switched my no-pour agar back to glass because of much less condensation.  I just ran through some clear agave nectar, and can't wait to test it out.


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OfflineStromriderM
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19312910 - 12/22/13 05:57 PM (10 years, 1 month ago)

:lol: that's hilarious. I also ran out of filtered lids today and had to go the tyvek route on a few. I was 3 short


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Stromrider]
    #19314251 - 12/22/13 11:56 PM (10 years, 1 month ago)

Quote:

Stromrider said:
:lol: that's hilarious. I also ran out of filtered lids today and had to go the tyvek route on a few. I was 3 short



I will be using 4x 46oz pickle jars in the very near future.


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19337084 - 12/28/13 01:29 PM (10 years, 1 month ago)

well....hows everyone coming along?
i got some more pda done yesterday tansfers done this morning and jars shaken up today and g2g tonight:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
    #19337133 - 12/28/13 01:43 PM (10 years, 1 month ago)

Aussie were incredibly tomentose from germination. They are now on there 3rd transfer and starting to look a little better. I am thinking about isolating 6 or so and running a bunch of little tubs side by side.

No growth yet from my purple mystic, hopefully I see germination soon. Made a few transfers from my PE and am hoping to see sectors soon there. Just put some pan cyan Jamaican on agar yesterday to so we shall see how they go.

On a non active note I also got some hericium coralloides and some shaggy mane wild spores on the tetra PDA as well, hope to see something from them soon too :wink:


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