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OfflineStromriderM
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: spacechildo]
    #19437124 - 01/18/14 10:10 AM (10 years, 13 days ago)

OK so the wild Texas tub is fully colonized and will be introduced into fruiting conditions today. The tub was just spawned 4 days ago :shocked: :rockon: Very aggressive. I like it.

I also just spawned a tub with my wild Aussie culture. This is from one of the prints that olive and bd was giving out that many of you have. Can't wait to see the fruits

Also my wikidzon grain master that I nocced up with an agar wedge on 12/31 is fully colonized now and I will be g2g that sometime this weekend as well :smile:

I may also have a couple other projects going on like a tub or 2 of gt and a tub or 2 of cambo :shrug: :lol:


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19438777 - 01/18/14 04:53 PM (10 years, 12 days ago)

Quote:

HypnotoadCroaked said:
Quote:

HypnotoadCroaked said:
I believe that I managed an isolation last week playing with a MS print to agar.  I pulled off a few xfers, with one being very close or perfect isolation.  I xfered from that to a few new plates and a chunk to a new master jar.






Master jar finished the other day.  Did a g2g out to 6 quarts. 



Been busy as a beaver, but wanted to promote myself and my objective :wink:

**Edit**
Master jar inoculated 12-27 via agar wedge and g2g today at 100%  It could have been done about 3-4 days ago.  Yes there is pieces of straw and shit in there.  I don't rinse out my strainer before I don't rinse my grain. 




I have 4-6 more days until I can spawn.  From the way things have progressed one jar is simply not keeping up.  5 qt is more than sufficient for a standard 66qt mono.  I have not decided on what mix of straw, coir and verm yet.  I am testing some straw right now, and though its a pain in the ass cutting in to pieces, it is as cheap as it gets.


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Invisiblerawrrock
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19441384 - 01/19/14 07:09 AM (10 years, 12 days ago)

G2G went smoothy until ONE GUY.. not so sure, but he's slowed down significantly. Metabs?







--------------------
:mushroom2:

60G tea trip report.


Edited by rawrrock (01/19/14 01:36 PM)


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: rawrrock]
    #19443757 - 01/19/14 05:42 PM (10 years, 11 days ago)

it's fucked, but no worries so is mine but it looks like i'll get a few out of my tub


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleFrankHorrigan
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19445431 - 01/19/14 11:49 PM (10 years, 11 days ago)

Quote:

HypnotoadCroaked said:
I believe that I managed an isolation last week playing with a MS print to agar.  I pulled off a few xfers, with one being very close or perfect isolation.  I xfered from that to a few new plates and a chunk to a new master jar.




Cubes usually take at least 4-5 transfers to even start to show sectoring. How many is a few?


--------------------

Yes, you can bump my old threads with a question.
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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: FrankHorrigan]
    #19445706 - 01/20/14 01:22 AM (10 years, 11 days ago)

Quote:

FrankHorrigan said:


Cubes usually take at least 4-5 transfers to even start to show sectoring. How many is a few?



IIRC it was 4 total xfers from MS.  There is no doubt that it is an mono-culture isolation, or so damn close I cannot tell.  There was no sectoring on any plate that has been xfered to.  Perfect circles of untested genetic makeup.  Ill know in a few weeks if it was worth it.


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OfflinePussyFart
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19445813 - 01/20/14 02:09 AM (10 years, 11 days ago)

If you started with spores, only made 4 transfers, and have not seen sectoring yet, you most likely do not have an isolate quite just yet.


Edited by PussyFart (01/20/14 02:09 AM)


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: PussyFart]
    #19447029 - 01/20/14 10:49 AM (10 years, 11 days ago)

Quote:

PussyFart said:
If you started with spores, only made 4 transfers, and have not seen sectoring yet, you most likely do not have an isolate quite just yet.



As I stated by the 4th transfer, there was no sectoring.  The 3rd xfer had a very few amount of sectors, and throughout the process I was only grabbing the smallest pieces.  Again I am 100% certain that the 5th xfer from MS created an isolated.  The 4th xfer, which went to grain and a few dishes I am nearly 100% sure that it was an isolate.  It did not make a perfect circle, but that could have been due to the angle that the inoculation wedge was placed. (on its side, not upside down/right side up).  Again, I would like to reiterate so that there is no confusion.  By the 5th xfer from MS, I am certainly dealing with isolated genetics.  Perfect circles, concentric rings (light rings?) and no tendril of mycelium is any farther away from the wedge than another.  My camera simply will not take pictures due to the "auto-focus" feature that cannot be disabled.  I would supply a photo to quell the concerns that you guys have, but it is like pissing in the wind with that camera.  Id rather get into an axe fight with an octopus in a phone booth than deal with that camera.


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InvisiblebodhisattaMDiscordReddit
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19447047 - 01/20/14 10:52 AM (10 years, 11 days ago)

Write something with a black sharpie on the dish so the autofocus has a target. chances are it's not an isolate though. I've had uniform circular growth after the 3-4th transfer and it was definitely not an isolate


Not an isolate.


Edited by Trusted cuItivator (01/20/14 10:54 AM)


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: bodhisatta]
    #19447080 - 01/20/14 11:04 AM (10 years, 11 days ago)

Quote:

bodhisatta said:
Write something with a black sharpie on the dish so the autofocus has a target.



I've tried similar trick with a bit of tape.  My camera focuses on the glass, and not what is in the glass.  (no-pour ball jars)  I should put an orange flag on a tooth pick inside of my jars, so that the camera has something interesting to focus on.

Debating isolate or not is moot.  I have no photo, and no way to supply one.  I can say that my 5th xfer dishes look very little like  your photograph.  Yours looks very 3 dimensional, where what I am looking at across 3 "plates" is a very flat and very uniform look.  It does not have the "3 dimensional" look that your photo has.  No matter what I have, it certainly is awesome.  If I can get a photo I will, otherwise we are simply beating a dead horse debating a moot point.


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InvisibleFrankHorrigan
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19447110 - 01/20/14 11:10 AM (10 years, 11 days ago)

It's probably close but not an isolate. Sometimes sectoring takes 6 or 7 transfers to really pop. Switching media for some of the plates also helps IMO.

If you want to test it's "isolate-ness," just start inoculating your grains with wedges AFTER you make your next transfers. Then you can keep going and see what happens, both on grains and on the agar media.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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AMU- Get an answer here -AMU


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InvisiblebodhisattaMDiscordReddit
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19447120 - 01/20/14 11:11 AM (10 years, 11 days ago)

morphology is dependent on the type of agar and % nutrition and temperature etc... You're really just looking for sectors. Chances are you haven't seen sectoring yet but would if you did 3-4 more transfers.


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: bodhisatta]
    #19447144 - 01/20/14 11:18 AM (10 years, 11 days ago)

fruit it out to ee whee your at and take a clone, you might end up with your mono culture from your first transfer from there:cool:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: FrankHorrigan]
    #19447175 - 01/20/14 11:24 AM (10 years, 11 days ago)

Quote:

FrankHorrigan said:
It's probably close but not an isolate. Sometimes sectoring takes 6 or 7 transfers to really pop. Switching media for some of the plates also helps IMO.

If you want to test it's "isolate-ness," just start inoculating your grains with wedges AFTER you make your next transfers. Then you can keep going and see what happens, both on grains and on the agar media.




You basically summarized my whole process.  ANYTIME I transfer anything, I keep the inoculate (In this case agar) with the transfer plate in isolation.  ANY contamination spotted on any of the plates means a total discard.  I have only had to destroy one "product line" in this manner. 


Frank And Pussyfart: 

I don't know what/how you obtain isolations.  My methods have been just as you described (If I read correctly).  I have been doing xfers to grain and to a plate.  Generally I do 2 plates per master jar PER xfer.  Example:  I take from my plate a wedge for the grain, One small wedge for one dish, and another wedge for a second dish.  I go to great lengths to take the smallest wedges possible. At that point I consider the donor plate "spent", but I will keep it for observation of contams for a few weeks.

I found that IF you let a no-pour jar fully colonize, mycelium will eventually start reaching up the edges.  It is VERY VERY easy to knife out a few tendrils right from the side of the glass and deliver them to a new plate. 

As for media, I certainly need to switch things up a bit.  I have been using the agave nectar since I started with this agar experiment.  I have a few of the "Brf+agar" plates up and running.  All of them survived the quarantine period issue free, so they got some wedges.  The real bitch with this formulation is that it is not transparent.  Basically I can't care less how they grow....I need transparency seeing how I have a lid on my jar. I will observe and report back in the appropriate journal.


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InvisiblebodhisattaMDiscordReddit
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19447190 - 01/20/14 11:28 AM (10 years, 11 days ago)

Quote:

ANY contamination spotted on any of the plates means a total discard.




Why?? agar is the only thing we have that is worth keeping with contams on it. We use it so that when we get contams it isn't a total discard. And you should be taking small wedges for transfers. Letting them grow out also means your mycelium cell linage is older and older. It's best to take transfers sooner rather than later so that when you do get an isolate it's as fresh as possible.

I've kept donor plates until they pinned in-vitro they'll eventually mature a mushroom and drop spores which you can use for extra clean swabs or really small prints since the shrooms will be really small.


Edited by Trusted cuItivator (01/20/14 11:30 AM)


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: bodhisatta]
    #19447303 - 01/20/14 12:01 PM (10 years, 11 days ago)

Quote:

bodhisatta said:
Quote:

ANY contamination spotted on any of the plates means a total discard.




Why?? agar is the only thing we have that is worth keeping with contams on it.



The amount of agar that I work with Is greatly disproportionate to the amount of grain that I will spawn.  I have had one plate get green mold for no reason that I can tell, so Ill blame the SAB.  Total loss after that incident was 3 plates.  I would rather work with 100% certainty than to fight a culture for clean xfers....especially when I have other plates that are not all crapped up.


Ill keep an eye on it, and take what you guys say into consideration.  I also looked through my collection, and found the 3rd-5th xfer plates.  The 3rd xfer is fully covering the agar, but looks lumpy and had no signs of sectoring.  The 4th xfer looks IDENTICAL to your photo Bodhisatta.  The xfer after that is the plate that I assume to be isolated.  I am waiting on the 6th xfers to grow out to verify.  It may or may not be isolated, but it certainly is making a very uniform circle with no parts being any farther away from center.

Cronicr:  I have 6 jars from the 5th xfer plate and I will be cloning something from that batch.  It should give me a chance to view the genetics from a different perspective.  I clone from EVERY tub, though most never make it back to grain, because I simply have more agar than I will ever need.


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19448679 - 01/20/14 05:13 PM (10 years, 10 days ago)

I know mine were not isolates but I only took 2 xfers.  I put them to grain a couple days ago and shook the shit outta then like you said hypno they are showing recovery!  I just put a few more prints on agar tonight


--------------------


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19452511 - 01/21/14 11:56 AM (10 years, 10 days ago)

Quote:

blueconfusion said:
I put them to grain a couple days ago and shook the shit outta then like you said hypno they are showing recovery!



:highfive1:


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InvisibleHypnotoadCroaked
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19463211 - 01/23/14 03:37 PM (10 years, 7 days ago)

For the first time since I started using a SAB, I have encountered a fail.  One out of 6 jars had been acting up since the process started.  It finally showed its true colors...which are an off-white/grey.  I don't know/care what it is, but it is not right.  All of the survivors are close to finished.  I would assume Late this weekend/early one evening next week I will be spawning to my new favorite Substrate....Straw.

I did a tub last week & This was my first experience with straw, and I simply have never seen myc move through something like they did with that.  It blew away the coir/verm tub right beside it that even had a 3 day head start.

5 jars remaining.  Ill report back next week sometime.  Have a great weekend all!


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19463248 - 01/23/14 03:44 PM (10 years, 7 days ago)

i'm sick as shit right now but my tub did indeed contaminate, gonna spawn a couple more once i feel better:cool:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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