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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 4 days, 23 hours
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
#19351179 - 12/31/13 01:28 PM (10 years, 30 days ago) |
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wouldn't be what it is without all you guys going on! i'm happy everyone is keeping it alive even if i have to take a minor break.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
#19353341 - 01/01/14 12:29 AM (10 years, 30 days ago) |
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 my 3 APE jars are pretty well done 27 qt mono build in the morning 5 qts of columbian rust spore behind it 66 qt build on the rise john allen strain grain to grain
 cyan clone on agar

 I EDITED MY OP HERE AS WELL ON PAGE ONE!HAPPY NEW YEARS ALL!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
Edited by cronicr (01/01/14 01:43 AM)
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
#19356154 - 01/01/14 08:32 PM (10 years, 29 days ago) |
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I ran myself out of jars over the last few weeks. Master jars are pint and a half wide mouths, and I had a jar of limited gene PE that was inoculated via agar 12-18, and has been at 100% for the last few days....I finally got a spare moment to do some G2G.......

4x 46oz pickle jars. I don't expect these to last forever, as they are probably not designed for pressure cooking. I am quite pleased that they survived the first cycle.
4x 46oz jars @ 3/4th filled = 138 oz 5x 32oz jars @ 3/4th filled = 120 oz
Each was delivered with approx 4oz of fully colonized grain. I expect one to fail because I accidentally touched a part of the inside of the lid, and in my movements lost track. Im giving that one a 50% chance. My gloves were still covered in ISO Alcohol, and success is possible, I won't loose sleep over the deal.
I am looking forward to trying to culture this PE again. My last attempt failed because I am at times a total dumbass.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
#19356692 - 01/02/14 12:00 AM (10 years, 29 days ago) |
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 i'm gonna go drop a wedge and shake the fuck out of it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
#19357502 - 01/02/14 07:27 AM (10 years, 29 days ago) |
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Quote:
cronicr said:
 i'm gonna go drop a wedge and shake the fuck out of it
this is something new for me. for some reason i thought when you drop a wedge you want it to colonize the top part of grain then shake. think i read that RR said if you shake it 1st it just knocks the mycelium off that agar and the mycelium doesn't recover, but the agar does recover which would prolong the wait.
good to know ill have to do this next time as i made since to me to shake the wedge.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
Edited by Yuri.Pono (01/02/14 07:28 AM)
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 1 hour, 3 minutes
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Yuri.Pono]
#19357510 - 01/02/14 07:36 AM (10 years, 29 days ago) |
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My wild Texas grain master is fully colonized and I just g2g it to 8 jars
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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 4 days, 23 hours
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Yuri.Pono]
#19357659 - 01/02/14 09:21 AM (10 years, 29 days ago) |
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Quote:
Yuri.Pono said:
Quote:
cronicr said:
 i'm gonna go drop a wedge and shake the fuck out of it
this is something new for me. for some reason i thought when you drop a wedge you want it to colonize the top part of grain then shake. think i read that RR said if you shake it 1st it just knocks the mycelium off that agar and the mycelium doesn't recover, but the agar does recover which would prolong the wait.
good to know ill have to do this next time as i made since to me to shake the wedge.
It was a suggestion by whippy I usually just try to drop a wedge in and bury it. Whippy asked us to try it and see what we thought.
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Yuri.Pono]
#19357715 - 01/02/14 09:42 AM (10 years, 29 days ago) |
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Quote:
Yuri.Pono said:
this is something new for me. for some reason i thought when you drop a wedge you want it to colonize the top part of grain then shake. think i read that RR said if you shake it 1st it just knocks the mycelium off that agar and the mycelium doesn't recover, but the agar does recover which would prolong the wait.
good to know ill have to do this next time as i made since to me to shake the wedge.
I have had 100% consistency with this, and no issues.

Here is a current example and some background:
12-18 I inoculated 4x of my master pint and a half jars with a PE wedge each. 2 of the jars I made every effort to obliterate the agar wedge. After this I did not shake these 2 jars again.
Now, I never ever marked which ones that I did this process with.
4 days ago, one jar was at 100%, and the jar on the right was about 90%, the other 2 were approaching the 20% mark.
So what happened:
Jar #1: http://www.shroomery.org/forums/showflat.php/Number/19356154#19356154 (2 posts up, click at your own risk). This was at 100% days ago......And I never shook it one time after the inoculation shake.
Jar #2: On the right. Never been shaken ever. Close to 100%, has a few spots that need time. Ill guess 97%.
Jar #3 & #4: On the left and not shown. When jar #1 hit 100% I shook this jar and jar #4. Jar #3 is at about 30% and jar #4 is probably at close to 20%.
Basically, I am seeing that I can make an agar wedge colonize a full jar in 10-15 days. My conditions are chilly at times, and I don't use an incubator. High 60f - Low 70f every day.
I am not concerned with what someone said will happen, because my eyes are wide open. This is my first documented example I submit. I have done this test myself about a dozen times, and it is 100% consistent. My agar wedges are not terribly big or small. Larger than I would use for another dish xfer, but this process I have been doing has only used one piece of agar. I don't see it as any different than running an agar wedge through a blender with water to make a LC. I figure if you shake a liquid culture all around, why not the agar.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Stromrider]
#19357723 - 01/02/14 09:47 AM (10 years, 29 days ago) |
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Quote:
Yuri.Pono said:
Quote:
cronicr said:
 i'm gonna go drop a wedge and shake the fuck out of it
this is something new for me. for some reason i thought when you drop a wedge you want it to colonize the top part of grain then shake. think i read that RR said if you shake it 1st it just knocks the mycelium off that agar and the mycelium doesn't recover, but the agar does recover which would prolong the wait.
good to know ill have to do this next time as i made since to me to shake the wedge.
lol yeah just someting i did at whippy's request, i used a generous wedge Quote:
Stromrider said: My wild Texas grain master is fully colonized and I just g2g it to 8 jars 
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
#19357762 - 01/02/14 10:04 AM (10 years, 29 days ago) |
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Quote:
cronicr said: lol yeah just someting i did at whippy's request, i used a generous wedge
Nice. Great shakes! Agar-Yatzee! I doubt that I will share this secret with the rest of the population. If they come here and find it, stellar.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
#19357766 - 01/02/14 10:08 AM (10 years, 29 days ago) |
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Quote:
Whippy said:
Quote:
cronicr said: lol yeah just someting i did at whippy's request, i used a generous wedge
Nice. Great shakes! Agar-YatzeeNAZZI! I doubt that I will share this secret with the rest of the population. If they come here and find it, stellar.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
Edited by cronicr (01/02/14 10:24 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
#19357884 - 01/02/14 10:53 AM (10 years, 29 days ago) |
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I have shaken the wedge around on several occasions and did find lots of knock points showed up. The only time that it bit me in the ass was when the wedge got stuck on the side of the glass near the top and I could not dislodge it. Ended up being ok, the mycelium just crawled down the glass and colonized the grains, just took a little longer.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Pastywhyte]
#19357909 - 01/02/14 11:00 AM (10 years, 29 days ago) |
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i would imagine something similar,after shaking the shit out of it i broke it up pretty good,also dropped a wedge from the same plate into one with out the shake
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
#19358084 - 01/02/14 11:57 AM (10 years, 29 days ago) |
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i shake after i drop my wedges, not agressively or anything but i knock it around worked fine everytime
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newera
Stranger


Registered: 03/05/13
Posts: 359
Last seen: 4 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: twistedty]
#19358147 - 01/02/14 12:08 PM (10 years, 29 days ago) |
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i once dropeed a wedge in and went to gop shake it and it got stuck to my pollyfill i shook the crap out of it after trying to get it loose but no luck,had to leave the jar sideways till some grain colonized lol
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: newera]
#19358229 - 01/02/14 12:26 PM (10 years, 29 days ago) |
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Quote:
newera said: i once dropeed a wedge in and went to gop shake it and it got stuck to my pollyfill i shook the crap out of it after trying to get it loose but no luck,had to leave the jar sideways till some grain colonized lol
I figure now is as good a time as any to reveal another thing I have done.....Unfortunately I can only find ONE of my lids as an example. I prefer plastic lids due to one piece construction. IF I have run out and need metal lids and rings, I keep the lid upside down because I hate to have it seal up on me, than having to pick it apart in a SAB. IF you ever had a lid get stuck so tight that you bend it in the SAB you understand. Grain goes flying everywhere followed by a symphony of expletives.
So I started using metal lids "upside down", and I got sick of seeing grains stuck in the polyfill inside the lid. This seemed to be a possible vector for contamination, as the polyfill is designed to catch baddies....mixing sterile grain with it seems bad.
I do my polyfill as tight as I can in 1/4th inch lid hole. I clip it back, and this is where it gets fun. I use a lighter to slowly heat the inside jar polyfill. I take my time and uniformly heat and when it all is gooey, I take another lid without holes and "squash" it down. If you are good, it makes a perfect circle. I have been doing this for months without issue. Don't heat the metal lid, just the polyfill.
Picts or it didn't happen:

Again, this is not a perfect model, but it shows you how it looks on the inside of the jar. So far this has worked great for no-pour and all my grain jars. Its still the same tight poly in the hole, just without "Don-King" inside my jars. EVERY jar of mine has this "feature"....Plastic mason lids and pp5 lids too.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
#19358381 - 01/02/14 12:57 PM (10 years, 29 days ago) |
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so you basicly turned it into easy felt
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
#19358405 - 01/02/14 01:02 PM (10 years, 29 days ago) |
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Quote:
cronicr said: so you basicly turned it into easy felt
Actually, the inside is a hard plastic nub. Solid as a rock. Ge happens now by flowing around the nub and out through and vice versa. The top still has the fluff, and the 1/4th inch hole still has fibrous poly. When I started doing this, I was melting both sides....After finding out that it didn't matter, I stuck with simply doing the inside. I hate the grains in the fluff on the inside. A logical solution would be to change to SFD etc, but again I am lazy and enjoy making the best of what I already have.....Plus this costs a whole lot less.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
#19358767 - 01/02/14 02:11 PM (10 years, 28 days ago) |
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FWIW I use polyfill stuffed super tight. I put my jars rubber side down since I've fucked with rubber side up and they just slide off like nothing in the SAB and then the lid is contaminated. I've never had to pry too hard to get them off rubber side down though. I guess that's just preference though. As for the polyfill the side that faces the inside of the jar is the bottom of a "V" shape or "U" shape of polyfill with the pointed end of the letter going through so that it's a smoother surface then the fluffy upside of the U or V that could trap grains or wedges.
So find out what works for you and stick with it is what I have to say.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: bodhisatta]
#19359081 - 01/02/14 03:19 PM (10 years, 28 days ago) |
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thats what i do, stuff it tight and forget about the dangly's, never have an issue with it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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