|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
newera
Stranger


Registered: 03/05/13
Posts: 359
Last seen: 4 years, 1 month
|
tiny prints
#19226767 - 12/04/13 01:42 PM (10 years, 2 months ago) |
|
|
well i ordered a pan cyan print from a sponsor and upon recieving it i was stoked, looked to be a decent size print, until yesterday when i took it to my sab and opened it up just to find inside the fisrt foil a tiny piece of foil cut to the size of about a dime with the tiny print on it, wtf do i do with this? i have a couple plates ready but can't imagine how i would use my loop on such a tiny piece of foil without it moving around seeing as i got no room to hold the print down normally i can take my inoculation loop and hold the print down byu the corner of the foil while i rub the print in a circular motion but this method may be impossible this time
|
TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
|
Re: tiny prints [Re: newera]
#19226874 - 12/04/13 02:03 PM (10 years, 2 months ago) |
|
|
Pans have tiny caps, therefore they have tiny prints. Ever try to mess around with an ATL#7 print? Same "problem." Just do your best to scrape some spores onto some agar.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
|
newera
Stranger


Registered: 03/05/13
Posts: 359
Last seen: 4 years, 1 month
|
|
i know the prints are tiny but normally there on a decent size piece of foil, this one was cut smaller then a dime right around the print. maybe i can stick a saftey pin through it to hold it down?
|
sharpshroomer98
Student of the Universe.



Registered: 08/30/11
Posts: 252
Last seen: 18 hours, 14 minutes
|
Re: tiny prints [Re: newera]
#19226915 - 12/04/13 02:14 PM (10 years, 2 months ago) |
|
|
Yeah TL, I was confused as you were but it makes sense now. There's just zero holding space without touching the print itself
-------------------- No, I wasn’t born in 98’
 
|
TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
|
|
I'd just hold it with my gloves & not worry about touching the print a bit.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
|
poopy mcpooperson
Pogonophobia



Registered: 10/17/13
Posts: 124
|
|
touch your heated loop to the agar first to cool it then touch your print so you have something to "grab" the spores with.
next, using a twisting/turning motion, lift the loop off the print and touch your agar.
heat and repeat.
-------------------- love and laughter
|
TheMustardTiger



Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 6 months
|
|
Quote:
poopy mcpooperson said: touch your heated loop to the agar first to cool it then touch your print so you have something to "grab" the spores with.
next, using a twisting/turning motion, lift the loop off the print and touch your agar.
heat and repeat.
--------------------
|
newera
Stranger


Registered: 03/05/13
Posts: 359
Last seen: 4 years, 1 month
|
|
all worked out ok i think, managed a few plates and have one with growth, thnx guys another quick q, should i bother trying to isolate something or just worry about getting a clean culture?
Edited by newera (12/10/13 01:19 PM)
|
TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
|
Re: tiny prints [Re: newera]
#19255430 - 12/10/13 01:28 PM (10 years, 2 months ago) |
|
|
I'd do both.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
|
newera
Stranger


Registered: 03/05/13
Posts: 359
Last seen: 4 years, 1 month
|
|
all right, i've done some isolating with cubes but pans i'm fairly new too. i did some plates of pans last year but never did more then a couple x-fers, i guess i'm just looking for the same sorta thing, nice even colonizing?
|
TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
|
Re: tiny prints [Re: newera]
#19255557 - 12/10/13 02:00 PM (10 years, 2 months ago) |
|
|
Yup. Transfer from the quickest growth.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
|
newera
Stranger


Registered: 03/05/13
Posts: 359
Last seen: 4 years, 1 month
|
|
|
newera
Stranger


Registered: 03/05/13
Posts: 359
Last seen: 4 years, 1 month
|
Re: tiny prints [Re: newera]
#19272886 - 12/13/13 11:03 PM (10 years, 2 months ago) |
|
|
made my first transfer tonight! was basicly just a blind transfer though, everything was pretty even and all looked the same so i made 4 transfers with one plate and i'm letting the others grow out,lost one to a contam that happened which couldn't be saved kinda fucked now though as i got no dishes left to make transfers and no money for agar for about 2 weeks......what to do now
|
|