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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Petri Plates
#19255027 - 12/10/13 11:53 AM (10 years, 1 month ago) |
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I just want to know what your favorite brand/style/size are.
I have been using two different brands myself, both 110mm and plastic.
Are there many here that use 60mm? If so why? I have never used these myself.
Thanks.
Before you tell me that I can find a thread just like this by searching I just want you to know that I already know that.
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 6 months, 18 days
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Re: Petri Plates [Re: Ganzig]
#19255091 - 12/10/13 12:05 PM (10 years, 1 month ago) |
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the smaller ones are nice for isolating strains away from contam or isolating strains in general as they use less agar. otherwise, whatever is cheap and vented. We are getting ours for 67.00$ a case on ebay and although they come in thinner, 4 mil sleeves than more expensive ones, they work great and we have had no issues thus far.
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thiotimoline
Stranger

Registered: 12/01/12
Posts: 898
Loc: Bay Area
Last seen: 6 years, 11 days
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I use 60mm Pyrex plates. They take up less space, which is good (I share a fridge with way too many other people). Using less agar is also an advantage, and I feel like they're less likely to break in shipping (doesn't apply to plastic obviously). But mainly it's that they were the first almost reasonably priced Pyrex plates I found for sale, I had no problems with them, and I wanted to standardize on one type.
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t3chnobily
Strangest


Registered: 02/04/12
Posts: 651
Loc: As Seen In VT
Last seen: 6 years, 9 months
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snag mini plates off of ebay as I need them. Eventually I'll find a deal on a set of divided pyrex dishes and go reusable.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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I really like the re-usable idea too.
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pseudotsuga


Registered: 06/29/11
Posts: 947
Loc: usa
Last seen: 4 years, 1 month
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Re: Petri Plates [Re: Ganzig]
#19255753 - 12/10/13 02:46 PM (10 years, 1 month ago) |
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I use half pint glass jars. I also like them because the seal works well enough that I haven't noticed any difference between those with parafilm and those without. I only use parafilm for long term storage of cultures now.
The only downside of using them has been having to clean them, if you use a lot the clean up could be a real problem.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Quote:
pseudotsuga said: I use half pint glass jars. I also like them because the seal works well enough that I haven't noticed any difference between those with parafilm and those without. I only use parafilm for long term storage of cultures now.
The only downside of using them has been having to clean them, if you use a lot the clean up could be a real problem.
So do I! Just made a batch.
For a minute I was using lids with a filtered air exchange port aka drilled hole with polyfil. But there was no need for that as there is a lot of air space in the jar.
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pseudotsuga


Registered: 06/29/11
Posts: 947
Loc: usa
Last seen: 4 years, 1 month
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Re: Petri Plates [Re: Ganzig]
#19255991 - 12/10/13 03:42 PM (10 years, 1 month ago) |
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Quote:
Ganzig said: So do I! Just made a batch.
yeah buddy! yep the filters are a waste o'time. You know I just sterilized a batch as too.
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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I use 60mm polypropylene dishes. Why? They are cheap, reusable, autoclavable and can't break. I can also fit a lot of them in a pressure cooker since I prepour them (don't have a flow hood).
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thiotimoline
Stranger

Registered: 12/01/12
Posts: 898
Loc: Bay Area
Last seen: 6 years, 11 days
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I used to use half-pint jars, but I can't fit many into my pressure cooker at a time, so that was a huge pain. It was nice to be able to pre-pour without having to worry about keeping them totally flat in the cooker, though.
liamtheloser: how does pouring before rather than after sterilizing the plates affect how many you can sterilize at a time?
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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It doesn't, I was referring to the 60mm size as to how many i can fit in a PC.
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CAP_TURTLE
Adventurer


Registered: 03/11/05
Posts: 1,135
Last seen: 5 years, 8 months
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I have seen a couple references to venting and enough space in the no pours to not need GE. I have been sealing my regular plates with parafilm right after inducing wedges and they have been growing stupid slow. I was told this is how to do it but if so why put ge holes. When i used ge holes on no pours and same formula I never had a problem. Can anyone elaborate on this? They were kind of thick too so I imagine there is not much air in there. Hopefully this is the problem and I can fix it but maybe using micropore til ready for long term storage.
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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if you don't have much air exchange they will grow very slow...
and $67/case? i'm never gonna buy from fischer sci again!
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Petri Plates [Re: drake89]
#19259392 - 12/11/13 10:01 AM (10 years, 1 month ago) |
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Quote:
drake89 said: and $67/case?
Yeah that is crazy. Can a brotha getta link?
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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nanncee


Registered: 12/01/12
Posts: 434
Loc: Utah
Last seen: 9 years, 1 month
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Quote:
CAP_TURTLE said: I have seen a couple references to venting and enough space in the no pours to not need GE. I have been sealing my regular plates with parafilm right after inducing wedges and they have been growing stupid slow. I was told this is how to do it but if so why put ge holes. When i used ge holes on no pours and same formula I never had a problem. Can anyone elaborate on this? They were kind of thick too so I imagine there is not much air in there. Hopefully this is the problem and I can fix it but maybe using micropore til ready for long term storage.
How much parafilm are you wrapping around it? I have been using the 2 inch wide stuff cut in half length wise then wrapping 2 times around them. Having no issues with growth speed or contams.
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 6 months, 18 days
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Quote:
CAP_TURTLE said: I have seen a couple references to venting and enough space in the no pours to not need GE. I have been sealing my regular plates with parafilm right after inducing wedges and they have been growing stupid slow. I was told this is how to do it but if so why put ge holes. When i used ge holes on no pours and same formula I never had a problem. Can anyone elaborate on this? They were kind of thick too so I imagine there is not much air in there. Hopefully this is the problem and I can fix it but maybe using micropore til ready for long term storage.
wrapping with parafilm will have no effect on rate of growth. Parafilm is designed to vent. Be sure to use vented plates though, and if you are having slow growth it is likely due to temp, weak strain, or poorly formulated agar. That said, no reason to go more than 1 1/4 times around each plate. I only double wrap when mailing a plate for added protection
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Quote:
Amanita virosa said:
Quote:
CAP_TURTLE said: I have seen a couple references to venting and enough space in the no pours to not need GE. I have been sealing my regular plates with parafilm right after inducing wedges and they have been growing stupid slow. I was told this is how to do it but if so why put ge holes. When i used ge holes on no pours and same formula I never had a problem. Can anyone elaborate on this? They were kind of thick too so I imagine there is not much air in there. Hopefully this is the problem and I can fix it but maybe using micropore til ready for long term storage.
wrapping with parafilm will have no effect on rate of growth. Parafilm is designed to vent. Be sure to use vented plates though, and if you are having slow growth it is likely due to temp, weak strain, or poorly formulated agar. That said, no reason to go more than 1 1/4 times around each plate. I only double wrap when mailing a plate for added protection
id say wrapping twice is a must. too many times i have lost plates cause of holes in the parafilm. but maybe i am stretching it too far...
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Petri Plates [Re: drake89]
#19260262 - 12/11/13 01:49 PM (10 years, 1 month ago) |
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I use 60mm vented disposable plates. They're cheap and save me agar and space.
If your parafilm is blocking GE, you're not stretching it enough.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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CAP_TURTLE
Adventurer


Registered: 03/11/05
Posts: 1,135
Last seen: 5 years, 8 months
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I stretch the parafilm to the limits. I also put them into bags. Maybe if I blow some air into it before sealing? I have been taking the air out before sealing. It is possible I don't ahve vented lids as well. I need tomake sure on that one and am about to do that now.
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CAP_TURTLE
Adventurer


Registered: 03/11/05
Posts: 1,135
Last seen: 5 years, 8 months
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Something else just occured to me. I used tap water this last time. I'm pretty sure in the past i used distilled cause I can't think of anything else I have used it for. Anyone ever had experiences with using tap water and their plates not fairing too well? Maybe whatever is used at the water treatment plant is detterring growth?
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