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Offlinegardenstate
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Beginner need a few things cleared up
    #19254767 - 12/10/13 10:50 AM (10 years, 1 month ago)

Swim is going to attempt to inoculate  20 vermiculite / brown rice flour 1/2 Pint Jars with 1 cc of spores per Jar, injected into 4 injection sites. Swim is mainly following The Magic Mushroom Grower's Guide 3.2 but had a few questions. I don't expect you guys to spoon feed me the information but if you could point me to some documents that might clear up my questions I would be stoked!

Question 1:
As far as contaminations go, should I be safe (at least for a majority) if I do the following:

1/2 Inch Dry Vermiculite placed on top as filter
Steam the Jars in a Pot of water with lid on for 1+ Hour (Tinfoil added to top)
Be careful when injecting heat needle, wear gloves mask ect.

Question 2:
Should I keep the jars in smaller groups away from each other to avoid cross contamination or is this unlikely?

Question 3:
After inoculation should the tinfoil be placed back over the holes? Should I use tape over the holes for the first week? Most guides are very confusing about this.

Question 4:
When substrate has fully colonized should I dunk the cake in water before fruiting?

I know these are noob questions but it seems like there are a lot of different opinions and I want to be sure

Thanks!


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OfflinecronicrFacebook
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19254791 - 12/10/13 10:54 AM (10 years, 1 month ago)

1)your good, 90 minutes is the standard for steaming pf jars
2)no they won't cross contam just try your best not to handle too often
3)after innocculation leave the foil off
4)yes dunk for 24 hours and roll


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePsychedelicjournal
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19254817 - 12/10/13 11:00 AM (10 years, 1 month ago)

I am still new to the site and growing personally. However, I do feel that i can at least give you a fellow noobs advice.

Question 1

I do believe that you should be fine. I am assuming you are using the PF-TEK (as a i) and it seems that you are right on call with the Tek. I would suggest, if you have the money, to get yourself a Pressure cooker. My first attempt at growing i used them and i did not yield a good percentage of non-contaminated jars. I would say a 60-70% yield of good jars. Now that i am using my pressure cooker, I have about a 80-90% yield. So perhaps, a later investment.

Question 2

From my understanding the layer on top of the substrate acts as a barrier from contamination . I put all my jars together and it doesn't seem to have any ill effect.

Question 3

I left my tin foil on for the first couple days then removed it. From my understanding, they have to have the tin foil off so there can be proper gas exchange.

Question 4

Yes. Dunk them in cold water for 24 hours. Make sure to wash them off and get that layer of protective verm off while washing. Dunk them for 24 hours and then roll them in verm. Go on with Tek from there.


--------------------
“I think of going to the grave without having a psychedelic experience like going to the grave without ever having sex. It means that you never figured out what it is all about. The mystery is in the body and the way the body works itself into nature.”
– Terence McKenna


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InvisiblebodhisattaMDiscordReddit
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Re: Beginner need a few things cleared up [Re: cronicr]
    #19254828 - 12/10/13 11:04 AM (10 years, 1 month ago)

Quote:

cronicr said:
1)your good, 90 minutes is the standard for steaming pf jars
2)no they won't cross contam just try your best not to handle too often
3)after innocculation leave the foil off
4)yes dunk for 24 hours and roll




:whathesaid:

I would give the let's grow mushrooms video a watch the PF tek part one. Type it in google and you'll find it. It covers the PF tek and every one of those 4 questions you asked. Check out the first thread in my signature too for some more tips.


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Offlinemongolian
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Re: Beginner need a few things cleared up [Re: bodhisatta]
    #19254845 - 12/10/13 11:08 AM (10 years, 1 month ago)

If you encounter mold contams, get a pc 0% contamination rate at first try :laugh:! (well one was, but I forgot the dry verm layer and bacteria colonized the top)


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OfflinecronicrFacebook
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Re: Beginner need a few things cleared up [Re: Psychedelicjournal]
    #19254848 - 12/10/13 11:09 AM (10 years, 1 month ago)

Quote:

Psychedelicjournal said:



Question 3

I left my tin foil on for the first couple days then removed it. From my understanding, they have to have the tin foil off so there can be proper gas exchange.

Question 4

Yes. Dunk them in cold water for 24 hours. Make sure to wash them off and get that layer of protective verm off while washing. Dunk them for 24 hours and then roll them in verm. Go on with Tek from there.



for the foil, it's more about your verm layer getting damp when you leave it on
and after the dunk be sure to rinse well, bacteria build up is common during the dunking


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinegardenstate
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Re: Beginner need a few things cleared up [Re: cronicr]
    #19254993 - 12/10/13 11:46 AM (10 years, 1 month ago)

Wow thanks so much everyone you really cleared things up!! Swim just purchased 24 half pint jars... Swim is waiting for his spores to come in the mail, should they prepare the jars now or wait so they can inoculate immediately after sterilizing and cooling? What is the shelf life of a spore syringe vs substrate jar? Swim would prefer to have them ready a day or two in advance but if this will compromise my sanitation Swim will wait. Swim really does want to invest in a PC eventually but for now their situation just will not allow it.


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OfflinecronicrFacebook
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19255004 - 12/10/13 11:48 AM (10 years, 1 month ago)

take your tinfoil hat off and knock the swim shit off, we got your back and the cake cops are not coming for ya:wink:, being the holidays i would just wait, never no when they will show up


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinegardenstate
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Re: Beginner need a few things cleared up [Re: cronicr]
    #19255037 - 12/10/13 11:55 AM (10 years, 1 month ago)

Quote:

cronicr said:
take your tinfoil hat off and knock the swim shit off, we got your back and the cake cops are not coming for ya;), being the holidays i would just wait, never no when they will show up




Lol never can be too safe! but it is annoying as hell sorry


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OfflinePsychedelicjournal
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19256669 - 12/10/13 05:58 PM (10 years, 1 month ago)

Keep the syringe in the fridge and it should be good for about 2 years.


--------------------
“I think of going to the grave without having a psychedelic experience like going to the grave without ever having sex. It means that you never figured out what it is all about. The mystery is in the body and the way the body works itself into nature.”
– Terence McKenna


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Offlinegardenstate
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Re: Beginner need a few things cleared up [Re: Psychedelicjournal]
    #19256942 - 12/10/13 06:46 PM (10 years, 1 month ago)

Quote:

Psychedelicjournal said:
Keep the syringe in the fridge and it should be good for about 2 years.



Oh ok thanks any idea at room temp (65) F
just might be awkward to have a needle full of spores in the fridge at my house in the situation Im in lol


Edited by gardenstate (12/10/13 06:47 PM)


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OfflinePsychedelicjournal
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19258840 - 12/11/13 05:51 AM (10 years, 1 month ago)

Room temp I have no idea. It might be best if you find a friend who doesn't mind having them in their fridge. If not i would scour this site for information


--------------------
“I think of going to the grave without having a psychedelic experience like going to the grave without ever having sex. It means that you never figured out what it is all about. The mystery is in the body and the way the body works itself into nature.”
– Terence McKenna


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Offlinegardenstate
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Re: Beginner need a few things cleared up [Re: Psychedelicjournal]
    #19259027 - 12/11/13 07:47 AM (10 years, 1 month ago)

Quote:

Psychedelicjournal said:
Room temp I have no idea. It might be best if you find a friend who doesn't mind having them in their fridge. If not i would scour this site for information




Might be able to get my bro to hold them but it would only be for a couple days?

EDIT:
I was wondering if there was anything I could do in the mean time? I ordered my spores still waiting for them to ship. I purchased my brf and verm, 24 half pint jars, and plan on drilling them today. I found a room that might be good for inoculation, it is the closet of our guest bedroom. It's a very small space with shelves and nothing in it.


Edited by gardenstate (12/11/13 10:53 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19259495 - 12/11/13 10:34 AM (10 years, 1 month ago)

don't use the sponsors name along with cultivation info....

room temperature is fine. Think about how these things get shipped to you... there's no temperature control from the heated warehouse to the cold mail delivery truck etc... Fridge for long time storage. Room temperature is fine I leave syringes in my myco tool bin for weeks since I hardly use spores for anything at all anymore and they do just fine.


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Offlinegardenstate
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Re: Beginner need a few things cleared up [Re: bodhisatta]
    #19259573 - 12/11/13 10:55 AM (10 years, 1 month ago)

Quote:

bodhisatta said:
don't use the sponsors name along with cultivation info....

room temperature is fine. Think about how these things get shipped to you... there's no temperature control from the heated warehouse to the cold mail delivery truck etc... Fridge for long time storage. Room temperature is fine I leave syringes in my myco tool bin for weeks since I hardly use spores for anything at all anymore and they do just fine.



Removed the info sorry that makes sense!

And thank so much I assumed that but I just want to make everything as perfect as possible! any other Ideas of ways I should prepare?


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InvisiblebodhisattaMDiscordReddit
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19259646 - 12/11/13 11:13 AM (10 years, 1 month ago)

I've never had a problem syringe inoculating my jars outside of a still air box but building one would be good for any future attempts at grain/agar. and it's better to syringe inoculate in a still air box anyhow.

In the meanwhile read through the user Notahacker420 and Frankhorrigan threads.


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Offlinegardenstate
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Re: Beginner need a few things cleared up [Re: bodhisatta]
    #19262843 - 12/11/13 09:18 PM (10 years, 1 month ago)

Quote:

bodhisatta said:
I've never had a problem syringe inoculating my jars outside of a still air box but building one would be good for any future attempts at grain/agar. and it's better to syringe inoculate in a still air box anyhow.

In the meanwhile read through the user Notahacker420 and Frankhorrigan threads.




thanks man I'll check em out


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OfflineCamtaro420
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Re: Beginner need a few things cleared up [Re: gardenstate]
    #19263193 - 12/11/13 10:14 PM (10 years, 1 month ago)

I'm new but they say a fresh trained mind is always on the ball.

1 - Yes and make sure to use a SAB on top of those methods. moving air = contaims I learned that the hard way just recently. Easy to make and cheap, I have also heard it is sometimes better to do 120 minutes of steam cleaning.

2 - That's kinda silly. you'll throw em out before they will fester up wherever you are keeping em as long as you're checking them. Keep em together and watch the temperature. Keep it perfect for mushrooms and not bacteria. Learned this the hard way too.

3 - If you're using the dry verm as a layer no foil after innoc. However a little paper micropore is probably ok from what I have learned and is what I will be doing when I do my cakes. Even though it is NOT necessary since the dry verm and the micropore tape are doing the same thing. I could just never feel comfortable with my cakes having a completely unprotected opening of some kind.

4 - Yes, big pot of TAP water. Boil it to purify. Let it cool. Dunk them in. Let em sit for 24+ hours, roll, wait.


--------------------


Edited by Camtaro420 (12/11/13 10:16 PM)


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