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OfflineBOOMERang27
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pressure cooker time
    #19253695 - 12/10/13 02:08 AM (10 years, 1 month ago)

I have a 12psi electric pressure cook and I am wondering how long it will take to sterilize my half pint jars


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OfflineLWB
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Re: pressure cooker time [Re: BOOMERang27]
    #19254097 - 12/10/13 06:35 AM (10 years, 1 month ago)

I have a digital pressure cooker and I put my BRF cakes on high for 45 mins and mine did fine. I'm not sure what the psi is for the one I have. Everything I have read online and watched on YouTube said use 15 psi.


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OfflineBucknaztee
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Re: pressure cooker time [Re: LWB]
    #19254141 - 12/10/13 06:54 AM (10 years, 1 month ago)

gee whiz at 12 PSI I would think at LEAST 60 minutes, but I'd say closer to 75 minutes. I always did PF jars for 90 minutes at 15 PSI, but the general conclusion around here seems to be that 60 minutes at 15 PSI is sufficient. Good Luck!


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OfflineRogerRabbitM
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Re: pressure cooker time [Re: Bucknaztee]
    #19254153 - 12/10/13 07:06 AM (10 years, 1 month ago)

30 to 45 minutes after reaching 12 psi is fine for 1/2 pints.  Be sure to leave the vent open at first to vent steam for a few minutes before closing it to build pressure.
RR


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OfflineBOOMERang27
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Re: pressure cooker time [Re: RogerRabbit]
    #19255842 - 12/10/13 03:08 PM (10 years, 1 month ago)

Quote:

RogerRabbit said:
30 to 45 minutes after reaching 12 psi is fine for 1/2 pints.  Be sure to leave the vent open at first to vent steam for a few minutes before closing it to build pressure.
RR




Thanks! That worked well. Does it matter that my lids still make the "click" sound? Thats because of the holes right?


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Offlinehongomagico
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Re: pressure cooker time [Re: BOOMERang27]
    #19255869 - 12/10/13 03:13 PM (10 years, 1 month ago)

i use four or five series of 15 minutes boils . letting an hour between them. it is what Tindalization is about.

supossedly spores of bacteria open up in those 1 hour letups and get killed afterwards with the next boil..


Edited by hongomagico (12/10/13 03:14 PM)


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OfflineKizzle
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Re: pressure cooker time [Re: hongomagico]
    #19256046 - 12/10/13 03:53 PM (10 years, 1 month ago)

Quote:

hongomagico said:
i use four or five series of 15 minutes boils . letting an hour between them. it is what Tindalization is about.

supossedly spores of bacteria open up in those 1 hour letups and get killed afterwards with the next boil..



Why? That's way more work.


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Offlinehongomagico
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Re: pressure cooker time [Re: Kizzle]
    #19256082 - 12/10/13 04:01 PM (10 years, 1 month ago)

yep. but it supposedly guarantees better sterilization which is what matters. it is not really much work , only time ..

it is taken from a biology book on growing bacteria strains.


Edited by hongomagico (12/10/13 04:02 PM)


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InvisibleFooMan
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Re: pressure cooker time [Re: hongomagico]
    #19256132 - 12/10/13 04:12 PM (10 years, 1 month ago)

You'd use tindalization for whole grains, if at all. It is totally unnecessary for the pf tek.


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OfflinecronicrFacebook
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Re: pressure cooker time [Re: FooMan]
    #19256140 - 12/10/13 04:15 PM (10 years, 1 month ago)

nice av foo!


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Offlinehongomagico
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Re: pressure cooker time [Re: cronicr]
    #19256159 - 12/10/13 04:18 PM (10 years, 1 month ago)

anyway tindalization is not much more expensive than plain boiling. it is easy just patience..


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OfflinecronicrFacebook
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Re: pressure cooker time [Re: hongomagico]
    #19256181 - 12/10/13 04:22 PM (10 years, 1 month ago)

Quote:

hongomagico said:
anyway tindalization is not much more expensive than plain boiling. it is easy just patienceuseless..



:laugh2:(i'm just fucking with ya...sorta)


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OfflineKizzle
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Re: pressure cooker time [Re: hongomagico]
    #19256189 - 12/10/13 04:24 PM (10 years, 1 month ago)

I doubt 15 minutes is even long enough for the substrate to reach boiling temperature. Not to mention an hour in between isn't long enough for endospores to germinate which is the main purpose for doing it like that.

I don't think you'd have any problem with the standard methods. Sometimes sterilization gets blamed for contamination when there are actually other causes of it.


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Offlinehongomagico
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Re: pressure cooker time [Re: Kizzle]
    #19256212 - 12/10/13 04:28 PM (10 years, 1 month ago)

i meant 15 minutes of boiling water killing bacteria . and you can let it rest more than an hour . the exact time depends on ambient temps..just until the boiler gets warm.

any way i do it like taht you can keep doing your thing..


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Offlinehongomagico
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Re: pressure cooker time [Re: hongomagico]
    #19256221 - 12/10/13 04:29 PM (10 years, 1 month ago)

http://www.shroomery.org/11235/Tyndallization

Tyndallization
How to Sterilize without a pressure cooker


If you dont have a pressure cooker (PC), or cant fit your substrate jars inside your PC (thats me), you can use a sterilization method called tyndallization.  Its a little faffy/time consuming but really keeps those contams at bay.

Tyndallization involves steaming your jars for 20mins, three times, on three consecutive days.  In-between  steaming, let the jars rest at room temp.

Basically some bacterial spores can survive high temperatures, but the heat shock will make them germinate - now they're vulnerable to the next steaming session.

Even late germinating spores will be killed-off during the third steam, leaving your substrate contam free : )

I like to steam for 45mins on the third occation just to be sure!

Happy growing


Edited by hongomagico (12/10/13 04:30 PM)


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Offlinehongomagico
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Re: pressure cooker time [Re: hongomagico]
    #19256239 - 12/10/13 04:32 PM (10 years, 1 month ago)

here he makes it 3 days . i have never done it so long . but 2 days yes sir..


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OfflinecronicrFacebook
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Re: pressure cooker time [Re: hongomagico]
    #19256249 - 12/10/13 04:33 PM (10 years, 1 month ago)

http://www.shroomery.org/forums/showflat.php/Number/17046622#17046622
do a quick search, it fails most of the time, steam for 8 hrs


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OfflineKizzle
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Re: pressure cooker time [Re: cronicr]
    #19256268 - 12/10/13 04:38 PM (10 years, 1 month ago)

That'd be silly to do for PF jars. You don't even need a PC for them. 90 minutes of steaming should give you 100% success.


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OfflinecronicrFacebook
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Re: pressure cooker time [Re: Kizzle]
    #19256274 - 12/10/13 04:39 PM (10 years, 1 month ago)

Quote:

Kizzle said:
That'd be silly to do for PF jars. You don't even need a PC for them. 90 minutes of steaming should give you 100% success.



also:whathesaid:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinehongomagico
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Re: pressure cooker time [Re: cronicr]
    #19256369 - 12/10/13 05:02 PM (10 years, 1 month ago)

pf jars dont have bacterial spores or what ? bacterial spores are more resistant than bacteria.


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OfflinecronicrFacebook
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Re: pressure cooker time [Re: hongomagico]
    #19256384 - 12/10/13 05:05 PM (10 years, 1 month ago)

the pf tek is normally too dry for major bacterial problems


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I'm tired do me a favor


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OfflineKizzle
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Re: pressure cooker time [Re: hongomagico]
    #19256442 - 12/10/13 05:17 PM (10 years, 1 month ago)

Quote:

hongomagico said:
pf jars dont have bacterial spores or what ? bacterial spores are more resistant than bacteria.



I believe it's because the greater density and water content allows the entire to heat up at reasonable rate. That's just my theory. For whatever reason, steaming works with them.


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Offlinehongomagico
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Re: pressure cooker time [Re: Kizzle]
    #19256455 - 12/10/13 05:20 PM (10 years, 1 month ago)

i do steaming ...it is only that only for 15 minutes . and then again..
letting a day pass and then another 15 minutes of steaming.


Edited by hongomagico (12/10/13 05:21 PM)


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OfflinecronicrFacebook
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Re: pressure cooker time [Re: hongomagico]
    #19256462 - 12/10/13 05:21 PM (10 years, 1 month ago)

Quote:

hongomagico said:
i do steaming ...it is only that only for 15 minutes . and then again..



theres....no need...to


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinehongomagico
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Re: pressure cooker time [Re: cronicr]
    #19256467 - 12/10/13 05:22 PM (10 years, 1 month ago)

if theres no need how come contamination happens ?


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OfflinecronicrFacebook
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Re: pressure cooker time [Re: hongomagico]
    #19256471 - 12/10/13 05:22 PM (10 years, 1 month ago)

your not doing it right:shrug:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKizzle
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Re: pressure cooker time [Re: hongomagico]
    #19256541 - 12/10/13 05:33 PM (10 years, 1 month ago)

Quote:

hongomagico said:
if theres no need how come contamination happens ?



What kind of contamination are you talking about?

There are few contaminants that have much chance of surviving even brief exposure to boiling temperatures. Generally 'under-sterilization' will result in Bacillus spp. of bacteria. Other contaminants are usually from contamination after sterilization.


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Offlinehongomagico
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Re: pressure cooker time [Re: Kizzle]
    #19256605 - 12/10/13 05:46 PM (10 years, 1 month ago)

i got black pointy bacteria sometimes..


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OfflineKizzle
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Re: pressure cooker time [Re: hongomagico]
    #19256655 - 12/10/13 05:56 PM (10 years, 1 month ago)

That sounds more like mold than bacteria. Most commonly it happens during the inoculation, often from contaminated spores when doing a spore inoculation but there are other ways depending on your inoculation procedure. It can also be the result of a damaged or wet filter/dry verm layer.


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InvisibleFooMan
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Re: pressure cooker time [Re: hongomagico]
    #19256933 - 12/10/13 06:45 PM (10 years, 1 month ago)

Quote:

hongomagico said:
i got black pointy bacteria sometimes..




Did you steam for a full 90 minutes? I used to only steam for 60 using the original pf tek and always got 100% success rate without a PC. Contamination can occur during inoculation or you could have had a contaminated spore syringe too.

Again, there is no need for fractional sterilization, but if you want to insist on taking 2 days to sterilize your jars go for it.


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