|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
BOOMERang27
Stranger

Registered: 12/09/13
Posts: 12
Last seen: 9 years, 11 months
|
pressure cooker time
#19253695 - 12/10/13 02:08 AM (10 years, 1 month ago) |
|
|
I have a 12psi electric pressure cook and I am wondering how long it will take to sterilize my half pint jars
|
LWB



Registered: 12/09/13
Posts: 26
Loc: Southern Girl
Last seen: 9 years, 3 months
|
|
I have a digital pressure cooker and I put my BRF cakes on high for 45 mins and mine did fine. I'm not sure what the psi is for the one I have. Everything I have read online and watched on YouTube said use 15 psi.
|
Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
|
Re: pressure cooker time [Re: LWB]
#19254141 - 12/10/13 06:54 AM (10 years, 1 month ago) |
|
|
gee whiz at 12 PSI I would think at LEAST 60 minutes, but I'd say closer to 75 minutes. I always did PF jars for 90 minutes at 15 PSI, but the general conclusion around here seems to be that 60 minutes at 15 PSI is sufficient. Good Luck!
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
|
Re: pressure cooker time [Re: Bucknaztee]
#19254153 - 12/10/13 07:06 AM (10 years, 1 month ago) |
|
|
30 to 45 minutes after reaching 12 psi is fine for 1/2 pints. Be sure to leave the vent open at first to vent steam for a few minutes before closing it to build pressure. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
BOOMERang27
Stranger

Registered: 12/09/13
Posts: 12
Last seen: 9 years, 11 months
|
|
Quote:
RogerRabbit said: 30 to 45 minutes after reaching 12 psi is fine for 1/2 pints. Be sure to leave the vent open at first to vent steam for a few minutes before closing it to build pressure. RR
Thanks! That worked well. Does it matter that my lids still make the "click" sound? Thats because of the holes right?
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
|
i use four or five series of 15 minutes boils . letting an hour between them. it is what Tindalization is about.
supossedly spores of bacteria open up in those 1 hour letups and get killed afterwards with the next boil..
Edited by hongomagico (12/10/13 03:14 PM)
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 10 hours, 52 minutes
|
|
Quote:
hongomagico said: i use four or five series of 15 minutes boils . letting an hour between them. it is what Tindalization is about.
supossedly spores of bacteria open up in those 1 hour letups and get killed afterwards with the next boil..
Why? That's way more work.
--------------------
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
Re: pressure cooker time [Re: Kizzle]
#19256082 - 12/10/13 04:01 PM (10 years, 1 month ago) |
|
|
yep. but it supposedly guarantees better sterilization which is what matters. it is not really much work , only time ..
it is taken from a biology book on growing bacteria strains.
Edited by hongomagico (12/10/13 04:02 PM)
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
|
You'd use tindalization for whole grains, if at all. It is totally unnecessary for the pf tek.
--------------------
Quick WBS Prep
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: pressure cooker time [Re: FooMan]
#19256140 - 12/10/13 04:15 PM (10 years, 1 month ago) |
|
|
nice av foo!
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
Re: pressure cooker time [Re: cronicr]
#19256159 - 12/10/13 04:18 PM (10 years, 1 month ago) |
|
|
anyway tindalization is not much more expensive than plain boiling. it is easy just patience..
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
Quote:
hongomagico said: anyway tindalization is not much more expensive than plain boiling. it is easy just patienceuseless..
(i'm just fucking with ya...sorta)
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 10 hours, 52 minutes
|
|
I doubt 15 minutes is even long enough for the substrate to reach boiling temperature. Not to mention an hour in between isn't long enough for endospores to germinate which is the main purpose for doing it like that.
I don't think you'd have any problem with the standard methods. Sometimes sterilization gets blamed for contamination when there are actually other causes of it.
--------------------
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
Re: pressure cooker time [Re: Kizzle]
#19256212 - 12/10/13 04:28 PM (10 years, 1 month ago) |
|
|
i meant 15 minutes of boiling water killing bacteria . and you can let it rest more than an hour . the exact time depends on ambient temps..just until the boiler gets warm.
any way i do it like taht you can keep doing your thing..
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
|
http://www.shroomery.org/11235/Tyndallization
Tyndallization How to Sterilize without a pressure cooker
If you dont have a pressure cooker (PC), or cant fit your substrate jars inside your PC (thats me), you can use a sterilization method called tyndallization. Its a little faffy/time consuming but really keeps those contams at bay.
Tyndallization involves steaming your jars for 20mins, three times, on three consecutive days. In-between steaming, let the jars rest at room temp.
Basically some bacterial spores can survive high temperatures, but the heat shock will make them germinate - now they're vulnerable to the next steaming session.
Even late germinating spores will be killed-off during the third steam, leaving your substrate contam free : )
I like to steam for 45mins on the third occation just to be sure!
Happy growing
Edited by hongomagico (12/10/13 04:30 PM)
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
|
here he makes it 3 days . i have never done it so long . but 2 days yes sir..
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 10 hours, 52 minutes
|
Re: pressure cooker time [Re: cronicr]
#19256268 - 12/10/13 04:38 PM (10 years, 1 month ago) |
|
|
That'd be silly to do for PF jars. You don't even need a PC for them. 90 minutes of steaming should give you 100% success.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: pressure cooker time [Re: Kizzle]
#19256274 - 12/10/13 04:39 PM (10 years, 1 month ago) |
|
|
Quote:
Kizzle said: That'd be silly to do for PF jars. You don't even need a PC for them. 90 minutes of steaming should give you 100% success.
also
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
Re: pressure cooker time [Re: cronicr]
#19256369 - 12/10/13 05:02 PM (10 years, 1 month ago) |
|
|
pf jars dont have bacterial spores or what ? bacterial spores are more resistant than bacteria.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
the pf tek is normally too dry for major bacterial problems
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 10 hours, 52 minutes
|
|
Quote:
hongomagico said: pf jars dont have bacterial spores or what ? bacterial spores are more resistant than bacteria.
I believe it's because the greater density and water content allows the entire to heat up at reasonable rate. That's just my theory. For whatever reason, steaming works with them.
--------------------
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
Re: pressure cooker time [Re: Kizzle]
#19256455 - 12/10/13 05:20 PM (10 years, 1 month ago) |
|
|
i do steaming ...it is only that only for 15 minutes . and then again.. letting a day pass and then another 15 minutes of steaming.
Edited by hongomagico (12/10/13 05:21 PM)
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
Quote:
hongomagico said: i do steaming ...it is only that only for 15 minutes . and then again..
theres....no need...to
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
Re: pressure cooker time [Re: cronicr]
#19256467 - 12/10/13 05:22 PM (10 years, 1 month ago) |
|
|
if theres no need how come contamination happens ?
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
your not doing it right
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 10 hours, 52 minutes
|
|
Quote:
hongomagico said: if theres no need how come contamination happens ?
What kind of contamination are you talking about?
There are few contaminants that have much chance of surviving even brief exposure to boiling temperatures. Generally 'under-sterilization' will result in Bacillus spp. of bacteria. Other contaminants are usually from contamination after sterilization.
--------------------
|
hongomagico
Stranger
Registered: 12/08/13
Posts: 142
Last seen: 3 years, 10 days
|
Re: pressure cooker time [Re: Kizzle]
#19256605 - 12/10/13 05:46 PM (10 years, 1 month ago) |
|
|
i got black pointy bacteria sometimes..
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 10 hours, 52 minutes
|
|
That sounds more like mold than bacteria. Most commonly it happens during the inoculation, often from contaminated spores when doing a spore inoculation but there are other ways depending on your inoculation procedure. It can also be the result of a damaged or wet filter/dry verm layer.
--------------------
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
|
Quote:
hongomagico said: i got black pointy bacteria sometimes..
Did you steam for a full 90 minutes? I used to only steam for 60 using the original pf tek and always got 100% success rate without a PC. Contamination can occur during inoculation or you could have had a contaminated spore syringe too. Again, there is no need for fractional sterilization, but if you want to insist on taking 2 days to sterilize your jars go for it.
--------------------
Quick WBS Prep
|
|