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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yea they are def. pp5, not tried nuking, don't own a microwave 
the reason I have been curious about NOT modifying these snap on PP5s is because the lid is loose enough that when u spin it, there is no resistance. but it must be air "restrictive" enough that no con tams enter...I have these (the PP5 in my last post) just sitting in my dining room with no contam, I smoke (in adjacent room, with door closed but drafty perimeter), have 2 cats and a dog that are within inches of my jars and PP5s..and they remain clean, even long term, like 3-4 weeks, like when I use them for mini grain isolate testers. I don't wanna jinx myself and say the L word...but...
any thoughts?
i might as well try it anyway, just need to get more, or wait till the ones I have grow enough to transfer.
random question to anyone... after u guys take transfers from a plate, whether petri, PP5, etc, do u generally just toss that sender plate? or mark it, and put in fridge for longer storage if indeed u got results that were intriguing...? if so, does this increase contam risk?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
Edited by blindingleaf (12/14/13 04:16 PM)
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Adustus
Multiple Personalities




Registered: 11/14/13
Posts: 521
Loc: USA
Last seen: 1 year, 6 months
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Now only if my damn roommate would wake up so I can bang some holes in these jars and get PCing my grains and agar. Ive only done my reishi so far. And even though I think I did a piss poor job my first time around vs contams(leaving jar open longer than I wanted). It seems the little guys fighting the good fight. How long till I can spot contams?
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Starting my mushrooms in some agar [Re: Adustus]
#19276088 - 12/14/13 07:02 PM (10 years, 1 month ago) |
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no telling when I guess. are you talking about con tams with agar? if so, then if they got on the plate while pouring or inoculating they will grow as fast or faster than the myc. if u r talking about grain jars/brf they can show at any time. i am unfamiliar with reishi myc and how it should/should not look, though I would def be interested in trying to grow it! PM me if u wanna trade something for it! I've never traded before, but I want to. I have actives in print form (just b+ and soon burma and peurto rican) and shiitaki and oyster cultures, both isolates. I don't even know how one might send cultures in the mail, but if i find out and u wanna trade, we should! I live in philly, so from here to new york is not too far! Im glad I posted on this thread, it seems like we both may be at the same level in the hobby experience wise, so it makes for good experience sharing btw us! maybe in five years we will be on Amanita virosa and forresters level giving ppl like us now good advice! we will grow up together on the shroomery haha!
ps... try this thread, there are a bunch of pages of just ppl posting pics of agar plates with edible and actives, I like looking through it occasionally when I'm bored, so maybe there will be some reishi pics on it. good luck! http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8328896/an//page//vc/1
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Adustus
Multiple Personalities




Registered: 11/14/13
Posts: 521
Loc: USA
Last seen: 1 year, 6 months
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Re: Starting my mushrooms in some agar [Re: blindingleaf]
#19276702 - 12/14/13 09:42 PM (10 years, 1 month ago) |
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Quote:
blindingleaf said: no telling when I guess. are you talking about con tams with agar? if so, then if they got on the plate while pouring or inoculating they will grow as fast or faster than the myc. if u r talking about grain jars/brf they can show at any time. i am unfamiliar with reishi myc and how it should/should not look, though I would def be interested in trying to grow it! PM me if u wanna trade something for it! I've never traded before, but I want to. I have actives in print form (just b+ and soon burma and peurto rican) and shiitaki and oyster cultures, both isolates. I don't even know how one might send cultures in the mail, but if i find out and u wanna trade, we should! I live in philly, so from here to new york is not too far! Im glad I posted on this thread, it seems like we both may be at the same level in the hobby experience wise, so it makes for good experience sharing btw us! maybe in five years we will be on Amanita virosa and forresters level giving ppl like us now good advice! we will grow up together on the shroomery haha!
ps... try this thread, there are a bunch of pages of just ppl posting pics of agar plates with edible and actives, I like looking through it occasionally when I'm bored, so maybe there will be some reishi pics on it. good luck! http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8328896/an//page//vc/1
Once its grown id love to send you a live culture on the house man. Ive only got my first agars going. Lol. So I'm prepping all my jars now and im using polyfil for gas exchange. Should i be using a clump big enough to cover the top or just the hole? And are they reusable?
On the note of how to send live cultures one member on here traded me for 8 live cultures in one go and sent me a small envelope fulled with mini plastic vessels with pop tops. He labeled each one with a roman numeral and included a ID card for each number. Shit was boss!
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Starting my mushrooms in some agar [Re: Adustus]
#19277925 - 12/15/13 06:35 AM (10 years, 1 month ago) |
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sweet! how big were the pop top tubes?
as far as poly, Ive never used it as a jar/agar filter, only used in mono tubs/ bucket grows. I know it does not cover the whole lid, just the GE hole. and probably reusable, but its so cheap that I would replace each time for peace of mind.
http://www.shroomery.org/forums/showflat.php/Number/18278624
that might be helpful for getting an image of how the poly should look with lids. are u talking about poly for grain jars? that tek also has an injection port, but u won't need it because u are doing an agar wedge inoculation correct? so just follow that tek instruction on the poly part of the lid, and then ur good to go! cover with foil during PC, and I even put a rubber band around foil below the lip of the jar so no steam hits anything.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 6 months, 17 days
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Re: Starting my mushrooms in some agar [Re: blindingleaf]
#19278334 - 12/15/13 09:22 AM (10 years, 1 month ago) |
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Quote:
blindingleaf said: yea they are def. pp5, not tried nuking, don't own a microwave 
the reason I have been curious about NOT modifying these snap on PP5s is because the lid is loose enough that when u spin it, there is no resistance. but it must be air "restrictive" enough that no con tams enter...I have these (the PP5 in my last post) just sitting in my dining room with no contam, I smoke (in adjacent room, with door closed but drafty perimeter), have 2 cats and a dog that are within inches of my jars and PP5s..and they remain clean, even long term, like 3-4 weeks, like when I use them for mini grain isolate testers. I don't wanna jinx myself and say the L word...but...
any thoughts?
i might as well try it anyway, just need to get more, or wait till the ones I have grow enough to transfer.
random question to anyone... after u guys take transfers from a plate, whether petri, PP5, etc, do u generally just toss that sender plate? or mark it, and put in fridge for longer storage if indeed u got results that were intriguing...? if so, does this increase contam risk?
I usually keep them around but I am working in front of a hood. I like to watch them for clues about contam. Since we sell cultures, I will often use a clean plate to fill orders over the course of a month. Another strategy that works well is to transfer wedges from your plate into individual micricentrifuge tubes. They come sterile. You can make thirty from one plate if ur careful. Them when you need a fresh plate just dump the wedge onto one.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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wow, never heard of those tubes! r they small? need to be kept in fridge? available on vendor sites? is that how shroomery members send live edible cultures in the mail for trading?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 5 hours
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Re: Starting my mushrooms in some agar [Re: blindingleaf]
#19279738 - 12/15/13 02:48 PM (10 years, 1 month ago) |
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Quote:
blindingleaf said: wow, never heard of those tubes! r they small? need to be kept in fridge? available on vendor sites? is that how shroomery members send live edible cultures in the mail for trading?
They're what a lot of people use for trading, they work great for it. Yeah, they're pretty small (1.5ml, I think there's other sizes too). Outgrow has them, that's where I got mine. A lot of other places carry them too.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Starting my mushrooms in some agar [Re: Forrester]
#19280154 - 12/15/13 04:41 PM (10 years, 1 month ago) |
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awesome, gotta check them out. how is the culture preserved when in the mail? is it just a small wedge that is usually mailed with h2o or something to that effect?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 5 hours
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Re: Starting my mushrooms in some agar [Re: blindingleaf]
#19280253 - 12/15/13 05:02 PM (10 years, 1 month ago) |
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Yep just an agar wedge, dropped into the tube, no water.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Adustus
Multiple Personalities




Registered: 11/14/13
Posts: 521
Loc: USA
Last seen: 1 year, 6 months
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Re: Starting my mushrooms in some agar [Re: Forrester]
#19282429 - 12/16/13 04:39 AM (10 years, 1 month ago) |
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Whats cool is they help reduce contams when inoculating a jar. Just pop it open and drop it in. Minimal exposure. Love it! And I can reuse the ones I have now to send live cultures to other people too 
I got my agar jars and my grain jars fitted with the GE filter using poly. PCing 3 jars of agar now. My reishi seems to be putting up a great fight against my epic fail with my agar. No contams so far but its taking a long time to take over. Is this normal?
I forgot the food coloring now that I think of it. But itd be stupid to stop it now and put it in. I should have a decent time spotting contams with the shroomery on my side though. Maybe I should just learn to spot contams on normal agar.
Fuck all this though im off to get some plates next time I get some money.
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