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Offlinebolbol
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Is 1 week of Incubation enough? [Newbie] (Updated current progress results)
    #19252511 - 12/09/13 08:53 PM (10 years, 1 month ago)

Ive had 4 small jars in an incubator for a week now. I saw first sign of mycelum growth in the 3rd day and by now one of the jars is white and has covered every spot on the BRF/verm substrate. Should I keep it in there for a longer period of time or should I get it out so it will start pinning?

Ok so 2 of the 4 small jars(including the very small jar that was included that I posted a picture of) were put in a bucket with only 2 holes at the top, covered in perlite at the bottom and the top covered with see through garbage. I didnt dunk and roll these cakes, I just added few bits of verm on the top of the cakes.

The other 2 jars were a bit bigger in size. I dunked them for 16 hours and sprinkled verm on all sides of it and placed it in a SGFC style bucket with holes on the bottom and the sides.

I gave them the same amount of care but the smallest cake that wasnt even dunked gave the most pins and has the biggest shrooms on it so far. The other ones have have very few probably 2-3, especially the two dunked and rolled that were placed on the SGFC.


Edited by bolbol (01/03/14 04:30 PM)


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OfflineTheMustardTiger
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19252516 - 12/09/13 08:54 PM (10 years, 1 month ago)

Pics


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InvisibleSuperFly
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19252528 - 12/09/13 08:56 PM (10 years, 1 month ago)

If you are going to fruit it as it is let it consolidate for a week after full colonization.

If you plan on crumbling it into bulk substrate then crumble at 100% colonization while the mycelium is fresh and young.


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SuperFly]
    #19252587 - 12/09/13 09:09 PM (10 years, 1 month ago)

http://i.imgur.com/OiwNfT1.jpg
http://i.imgur.com/0VEfSCD.jpg
http://i.imgur.com/eo1iXsA.jpg
http://i.imgur.com/9BUqId0.jpg
http://i.imgur.com/8xJCM1o.jpg
http://i.imgur.com/1wmbWsU.jpg

Here are the pics.
I want to get it out of the jars when I see pinnings to put them in another container for them to grow. Im following the PFtek


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OfflineTheMustardTiger
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19252604 - 12/09/13 09:12 PM (10 years, 1 month ago)

Dry Verm Layer? Ditch that foil bro. I'm surprised those colonized as quickly as you said.


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OfflineSamhainJ
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SuperFly]
    #19252608 - 12/09/13 09:13 PM (10 years, 1 month ago)

Quote:

TheMrMACdaddy said:
If you are going to fruit it as it is let it consolidate for a week after full colonization.

If you plan on crumbling it into bulk substrate then crumble at 100% colonization while the mycelium is fresh and young.




:whathesaid: A week seems kinda quick for jars to be fully colonized.. even working with brf bags the quickest ive had em fully colonize was 10 days, and bags can be mashed which speeds colonization up like 5 times compared to brf jars. im guessing just the outside of the substrate is colonized, and you dont wanna try to rush it and have em contam..besides, if the middle isnt colonized the cakes most likely wont pin bc there is still sub to colonize.. i would gve em at least another 6 days even if your sure they arw colonized all the way through so they consolidate..

even if you are gonna crumble and case em with 50/50 etc or sawn to a mono i think you better give em another week.. better to wait than have your work turn green!


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Edited by SamhainJ (12/09/13 09:19 PM)


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OfflineSamhainJ
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SamhainJ]
    #19252617 - 12/09/13 09:15 PM (10 years, 1 month ago)

What made you decide to use shot glasses? Was it a test or practice run? I dont see any other reason for wasting the spores on 1.5 oz cakes unless testing syringe you made or something bc your not gonna get much at all even if you case all of em..


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OfflineTheMustardTiger
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Re: Is 1 week of Incubation enough? [Newbie] [Re: TheMustardTiger]
    #19252619 - 12/09/13 09:15 PM (10 years, 1 month ago)

Upon further review, those jars are tiny! What ratio of verm/BRF did you use? This partially explains the quick colonization


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OfflineTheMustardTiger
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SamhainJ]
    #19252628 - 12/09/13 09:16 PM (10 years, 1 month ago)

Quote:

SamhainJ said:
What made you decide to use shot glasses? Was it a test or practice run? I dont see any other reason for wasting the spores on 1.5 oz cakes unless testing syringe you made or something bc your not gonna get much at all even if you case all of em..




:thumbup: Invest in some 1/2 pint jars. Very cheap.


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SamhainJ]
    #19252631 - 12/09/13 09:17 PM (10 years, 1 month ago)

Well the actual problem is that this is my first time and I don't know if the middle part is colonized or not. I didnt use premade stuff, I just added verm + brown rice flour that I made myself. I topped it with dry verm and put it there last sunday night.


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OfflineTheMustardTiger
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19252639 - 12/09/13 09:20 PM (10 years, 1 month ago)

Quote:

bolbol said:
Well the actual problem is that this is my first time and I don't know if the middle part is colonized or not. I didnt use premade stuff, I just added verm + brown rice flour that I made myself. I topped it with dry verm and put it there last sunday night.




At the rate your jars are going, consolidate for a few days after full colonization. I'm interested to see the yield. Anyone care to place a wager on how many actual fruit bodies end up on these little guys? Make sure you post pics of pinning.


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: TheMustardTiger]
    #19252647 - 12/09/13 09:21 PM (10 years, 1 month ago)

I used tiny glasses to test out how the incubator does its job and if my sterilization technique worked, so basically it was a test to see if everything is fine. I added bigger jars later on once I saw that all the 4 jars showed no signs of contamination.

The verm to brf ratio is 3.5 to 1


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OfflineOgreLokon
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Re: Is 1 week of Incubation enough? [Newbie] [Re: TheMustardTiger]
    #19252651 - 12/09/13 09:22 PM (10 years, 1 month ago)

Those are ADORABLE! I wanna know how this turns out, as well!


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OfflineSamhainJ
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19252670 - 12/09/13 09:24 PM (10 years, 1 month ago)

Id be willing to bet that even as small as they are, the middles arent colonized.. i noticed a few lil spots on em in your pics that look like some arent even completely done on the outside.. i would def case all em using frankhorrigans 50/50 tek and maybe you can pull 1-4g dry depending on the weight of all of your shot glass cakes vombined..either way give em more time and dont forget to start some new half pint jars so you wont have to wait a month after yoyr shotglass cakes are spent.. always have jars colonizing bc after you succesfully grow the first time your gonna be addicted to growing em.. i enjoy growing even more than eating em lol


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OfflineSamhainJ
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SamhainJ]
    #19252712 - 12/09/13 09:31 PM (10 years, 1 month ago)

Unless yoyr house is 65°F or colder a incubator isnt neccasary.. i do put my jars and bags in a room at my grow house that averages 75° and gets as high as 80°F.. but temps in the 80s increase the risk of germinating endosporest that survived sterilization.. cooler temps are less likely to germinate dormant contams


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SamhainJ]
    #19252736 - 12/09/13 09:34 PM (10 years, 1 month ago)

It is colder than 65.. I had the thermostat on 28 C and ive had couple of times when I checked on it and it was unplugged but it definitely didnt stay without a thermostat heating it up for more than 8 hours.


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OfflineTheMustardTiger
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SamhainJ]
    #19252748 - 12/09/13 09:35 PM (10 years, 1 month ago)

Quote:

SamhainJ said:
Unless yoyr house is 65°F or colder a incubator isnt neccasary.. i do put my jars and bags in a room at my grow house that averages 75° and gets as high as 80°F.. but temps in the 80s increase the risk of germinating endosporest that survived sterilization.. cooler temps are less likely to germinate dormant contams



A Portable electric oil heater on wheels and a bedroom is the best incubator I've ever used.


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OfflineSamhainJ
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Re: Is 1 week of Incubation enough? [Newbie] [Re: TheMustardTiger]
    #19252774 - 12/09/13 09:40 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
Quote:

SamhainJ said:
Unless yoyr house is 65°F or colder a incubator isnt neccasary.. i do put my jars and bags in a room at my grow house that averages 75° and gets as high as 80°F.. but temps in the 80s increase the risk of germinating endosporest that survived sterilization.. cooler temps are less likely to germinate dormant contams



A Portable electric oil heater on wheels and a bedroom is the best incubator I've ever used.



I hear these arw the best way to incubate if you have to get temps up.. just dont use heat pads etc like alot of noobs use,bc they dry the substrate out and are possible fire hazards


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OfflineTheMustardTiger
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Re: Is 1 week of Incubation enough? [Newbie] [Re: SamhainJ]
    #19252784 - 12/09/13 09:42 PM (10 years, 1 month ago)

Quote:

SamhainJ said:
Quote:

MustardTiger66 said:
Quote:

SamhainJ said:
Unless yoyr house is 65°F or colder a incubator isnt neccasary.. i do put my jars and bags in a room at my grow house that averages 75° and gets as high as 80°F.. but temps in the 80s increase the risk of germinating endosporest that survived sterilization.. cooler temps are less likely to germinate dormant contams



A Portable electric oil heater on wheels and a bedroom is the best incubator I've ever used.



I hear these arw the best way to incubate if you have to get temps up.. just dont use heat pads etc like alot of noobs use,bc they dry the substrate out and are possible fire hazards



Truth. Also, the electric oil heaters don't mess with the RH too much which I enjoy for a variety of reasons.


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Offlinetwistedty
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Re: Is 1 week of Incubation enough? [Newbie] [Re: TheMustardTiger]
    #19252838 - 12/09/13 09:51 PM (10 years, 1 month ago)

you want to heat the whole room not just the container its in.

like Phil Collins said an oil radiant heater in the room is your best friend


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: twistedty]
    #19298088 - 12/19/13 01:43 PM (10 years, 1 month ago)

After letting it consolidate for a week, Ive taken it out to initiate pinning. Its the 5th day that its been out and I havent seen any signs of mushrooms through the glass. I was wondering if it has anything to do with the temperature. The temperature of the house is kind of low and I was wondering if that may be the problem or I just havent waited long enough?


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Offline1down5up
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19298154 - 12/19/13 02:03 PM (10 years, 1 month ago)

What is your temp?  No higher than 80 and anywhere in the mid to highn70's is good.
I'd say just wait it out, if it's not in trouble, just leave it.


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: 1down5up]
    #19298221 - 12/19/13 02:19 PM (10 years, 1 month ago)

its around 65...


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OfflinePussyFart
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19298752 - 12/19/13 04:33 PM (10 years, 1 month ago)

Quote:

bolbol said:
its around 65...



That's fine....sometimes this just takes time.....


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: PussyFart]
    #19302929 - 12/20/13 01:29 PM (10 years, 1 month ago)

also I have a question..
If i place a fully colonized jar in the incubator set at 80 F which I use for jar colonization, will it start pinning? or having lights and lower temp is a must?


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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19303149 - 12/20/13 02:18 PM (10 years, 1 month ago)

it may start pining but it's not an issue


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OfflinePussyFart
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19303277 - 12/20/13 02:48 PM (10 years, 1 month ago)

Quote:

bolbol said:
also I have a question..
If i place a fully colonized jar in the incubator set at 80 F which I use for jar colonization, will it start pinning? or having lights and lower temp is a must?



Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.


Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR


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Offlinesharpshroomer98
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Re: Is 1 week of Incubation enough? [Newbie] [Re: PussyFart]
    #19303312 - 12/20/13 02:55 PM (10 years, 1 month ago)

I swear NAH, I see you reference this every day :lol:

I have to say kudos for being able to implement this to many a different instances.


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: PussyFart]
    #19303435 - 12/20/13 03:18 PM (10 years, 1 month ago)

Thanks a lot for the tips!
I will just put them all under light from the pm to am and have them in the incubator from am to pm and see how that goes


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19314343 - 12/23/13 12:42 AM (10 years, 1 month ago)

For the other jars I have ready I want to dunk them and also try the freeze over night technique. Should I freeze it first then dunk the next day?


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OfflinePussyFart
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19314357 - 12/23/13 12:56 AM (10 years, 1 month ago)

Cold shocking is not beneficial to tropical species.


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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19314363 - 12/23/13 12:57 AM (10 years, 1 month ago)

Absolutely not. Scrape the dry verm layer in the trash. Birth. Rinse. Dunk for 12-24 hrs. Roll in dry verm. Place in FC (with cakes should be an SGFC I believe.)

EDIT: My apologies.
Mist till verm is saturated. Fan. Evaporation is major pinning trigger. Mist when verm looks dry. Fan after. Do this till harvest. Let dry for few days. Dunk. Repeat.

:whathesaid:


Edited by magickspore (12/23/13 01:19 AM)


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Re: Is 1 week of Incubation enough? [Newbie] [Re: magickspore]
    #19314375 - 12/23/13 01:04 AM (10 years, 1 month ago)

No after the flush you let it dry a bit then dunk


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: cronicr]
    #19314393 - 12/23/13 01:15 AM (10 years, 1 month ago)

the ones I currently have havent been rolled in dry verm. Is that okay or does it make a lot of difference? How long should I fan them for?


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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] *DELETED* [Re: bolbol]
    #19328888 - 12/26/13 01:30 PM (10 years, 1 month ago)

Post deleted by bolbol

Reason for deletion: irrelevant



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Offlinebolbol
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Re: Is 1 week of Incubation enough? [Newbie] [Re: bolbol]
    #19364205 - 01/03/14 04:30 PM (10 years, 27 days ago)

http://i39.tinypic.com/duyxy.jpg
this is the picture, info is added on the first post


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