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Offlineblueconfusion
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Just say NO to LC and YES to PDYA
    #19249080 - 12/09/13 08:00 AM (10 years, 2 months ago)

I'm making about 2000 mL. of PDYA (Potato Dextrose Yeast Agar), but I'll break it down to 500 mL. measurements I just like to make a lot at once fill the Pressure Cooker and always have some on hand. :shrug:

WHAT YOU'LL NEED
AGAR (order this online, find it in the local health food shop or asian market)
POTATO (I just use Russets, I know some people use potato flakes or potato starch)
DEXTROSE (this is corn sugar, i know there are substitutions such as karo syrup or honey, but if you have a local brewer's shop it's easy to find)
NUTRITIONAL YEAST[OPTIONAL] (this can also usually be found in a health food store i actually found mine in the health food isle at Kroger)

INSTRUCTIONS
Boil about 500 mL. (add a little more to allow for evaporation) per 150 grams of peeled and diced potatoes for about 30 minutes.
 
Then strain the potato water through a cheese cloth or fine mesh strainer.
 
Next simmer 9 grams of Dextrose and 1 gram of yeast in the water until they dissolve in the potato water.  Simmer on low and stir occasionally i never really let it boil i just let it go on low for longer.
 
Turn off the heat off and slowly add 9 grams of agar stirring until it dissolves.  The agar will try to clump so be sure to add it very slowly and stir little bits at a time.
 
Then pour the PDYA into the receiving vessel.  (I use Snapple bottles with a 1/4" hole drilled in the lid and poly-fill stuffed tightly in the hole; to stuff the poly i take a cotton ball sized piece of poly fold it in half twist it and stuff it in with a stick and then cut off the excess with a pair of scissors).



to avoid a mess use a funnel, and for Snapple bottles I generally pour about 250 mL. per jar just to keep an even and consistent pour and so i don't have to worry about the agar boiling over in the jar.

Cover the lid tightly with aluminum foil and place in the pressure cooker for 45 minutes @ 15 psi (follow your pressure cooker instructions for pressure cooking).


what i do with the foil is tear about a 3" strip rip that in half and fold into quarters, i place over the lid and try to tuck it in underneath the lid. 
Allow your pressure cooker to drop pressure naturally and allow the bottles to cool slightly around 160 degrees (this is just an estimate) and either pour your dishes or allow to fully cool and store in the fridge.

If you are pouring right way prep your SAB with all your dishes, agar bottles and parafilm or saran wrap.  Allow the air to settle in your SAB for at least an hour.
Be sure to swirl NOT SHAKE your jars when you pull them out of the pressure cooker as the ingredients may have settle continue to do this lightly swirling in a circular motion until they are cool enough to put in your SAB and pour. 

If you are reheating on the stove put in a stock pot or pressure cooker with something on the bottom to not burn the agar, and i cook on a low-med setting and allow the hot water bath to evenly reheat the jars.  Same thing as above be sure to swirl your jars!

Open your petri dish sleeve in the SAB and stack the dishes
Now working smoothly pour your dishes with about a 1/4" of agar working from top to bottom.
Allow the dishes to cool overnight in the SAB.
Now inoculate with a piece of colonized grain, a piece of clone tissue, a drop of spore solution, or print.
Wrap with parafilm or saran wrap and label with date and species.

IT'S REALLY THAT EASY!!!
the extra poured dishes that i won't use go into a gallon ziploc baggy with date and label in the fridge




Edited by blueconfusion (12/13/13 02:28 PM)


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InvisibleTiN 42
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19249110 - 12/09/13 08:13 AM (10 years, 2 months ago)

Sounds good I'll have to try. you don't PC the dishes ?


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: TiN 42]
    #19249116 - 12/09/13 08:15 AM (10 years, 2 months ago)

no i use plastic disposable ones, i just leave them in the sleeve until right before I pour, I open them in the SAB.


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InvisibleTiN 42
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19249120 - 12/09/13 08:17 AM (10 years, 2 months ago)

Right on thanks.


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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: TiN 42]
    #19249175 - 12/09/13 08:50 AM (10 years, 2 months ago)

I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19249194 - 12/09/13 08:56 AM (10 years, 2 months ago)

I haven't been doing agar long but i have seen a little better growth outta the PDYA.  I haven't actually done a side by side of PDA and PDYA, but the growth seems IMO to be more aggressive.


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19249366 - 12/09/13 10:04 AM (10 years, 2 months ago)

i actually ended up with about 1750 mL of agar going into jars, i started with about 2300 mL. of water boiling the potatoes.


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InvisiblePestile

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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19249670 - 12/09/13 11:29 AM (10 years, 2 months ago)

Where do you store the excess media bottles and how do you heat them up again?


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InvisibleSpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19249723 - 12/09/13 11:43 AM (10 years, 2 months ago)

I used to use Snapple bottles too. They work great.


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pestile]
    #19249784 - 12/09/13 11:57 AM (10 years, 2 months ago)

Quote:

Pestilence said:
Where do you store the excess media bottles and how do you heat them up again?



I usually just keep the foil on them put them in gallon ziploc baggies and throw them in the fridge.  I haven't had any problems with this yet.  To reheat them I use my PC with the tray on the bottom and fill it with water to about half way up the jars, i don't bring it to a boil i'm just looking to liquefy not recook the agar.


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
    #19251172 - 12/09/13 04:51 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
I used to use Snapple bottles too. They work great.



i don't think i had ever seen your write up Jedi, or if i had it was when i was still scared to do agar, what a fool i was :rofl: agar is so easy!


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Offlinetwistedty
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19251185 - 12/09/13 04:54 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?




i use pda also its just add water and pc and pour.  but from my understanding myc likes when you switch up the mix after a few transfers or somehting of that nature.

i havent ran into this problem yet so i havent looked into to far


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19251188 - 12/09/13 04:55 PM (10 years, 2 months ago)

also that is a shit TON of agar how many dishes you gonna make son :lmafo:


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19251201 - 12/09/13 04:58 PM (10 years, 2 months ago)

Quote:

twistedty said:
also that is a shit TON of agar how many dishes you gonna make son :lmafo:



well i have a few friends sending me some prints, plus i have my own isolates i'm working on so...  as many as i can! :rofl:


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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19251671 - 12/09/13 06:20 PM (10 years, 2 months ago)

Quote:

twistedty said:
Quote:

Pastywhyte said:
I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?




i use pda also its just add water and pc and pour.  but from my understanding myc likes when you switch up the mix after a few transfers or something of that nature.

i haven't ran into this problem yet so i havent looked into to far




Yeah I use MEA for my slants and for my plates I try to rotate PDA, grain soak water, and dog food. Been reading fastfreds cookbook a lot lately and thinkin bout throwing a few new recipes into the mix. Gonna do some MEA pour plates up soon (got a few sleeves o the X ones) with a little tetracycline, got a lot of wild prints to attend to. Shit I'm seeing agar potential everywhere these days. Poured myself a bowl of rice krispies and thought wait a sec. . . :lol:


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Offlinetwistedty
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19251687 - 12/09/13 06:24 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

twistedty said:
Quote:

Pastywhyte said:
I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?




i use pda also its just add water and pc and pour.  but from my understanding myc likes when you switch up the mix after a few transfers or something of that nature.

i haven't ran into this problem yet so i havent looked into to far




Yeah I use MEA for my slants and for my plates I try to rotate PDA, grain soak water, and dog food. Been reading fastfreds cookbook a lot lately and thinkin bout throwing a few new recipes into the mix. Gonna do some MEA pour plates up soon (got a few sleeves o the X ones) with a little tetracycline, got a lot of wild prints to attend to. Shit I'm seeing agar potential everywhere these days. Poured myself a bowl of rice krispies and thought wait a sec. . . :lol:




ya myc loves to thrive, there is pic somewhere round here where a mushroom fruited in a syringe and started coming out of the needle.

apparently the dude forgot about it for along time.

ive seen em growing out of a garbage disposal in a sink also on another site.

mycelium just doesnt give a fuck




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Offlinetwistedty
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19251697 - 12/09/13 06:25 PM (10 years, 2 months ago)



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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19251733 - 12/09/13 06:30 PM (10 years, 2 months ago)

the very first time i tried agar was the BRF substitute no pour in 1/4 pint jelly jars that was about a year ago i kinda gave up for a while because of my limited success there. 
this time i made PDA and MEA, but then FH encouraged me to add the yeast so i did and it really does work great.  Might try a MYA soon as well.  I have also been reading through a lot of the cookbooks, you can mix about anything and mycelium will grow.


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19251741 - 12/09/13 06:31 PM (10 years, 2 months ago)

Quote:

blueconfusion said:
the very first time i tried agar was the BRF substitute no pour in 1/4 pint jelly jars that was about a year ago i kinda gave up for a while because of my limited success there. 
this time i made PDA and MEA, but then FH encouraged me to add the yeast so i did and it really does work great.  Might try a MYA soon as well.  I have also been reading through a lot of the cookbooks, you can mix about anything and mycelium will grow.




my point exactly i mean, dog food grain water etc.  myc is amazing


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19251747 - 12/09/13 06:32 PM (10 years, 2 months ago)

hey pasty how much tetracycline you add?  i have some wild prints on the way


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