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OfflineBassbassik
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Registered: 12/03/13
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Aggressive mycelium in contam jars doing great?
    #19238096 - 12/06/13 07:32 PM (10 years, 2 months ago)

AFOAF noc'd 12 Pint jars of WBS with B+ MS, in a sealed glove box on 11/19. 7 of them showed contamination at the inoculation point. The mold appears to be bread mold or a variant of dark grayish/green mold similar to some penicillium. This mold is slow to spread and doesn't seem to be very aggressive.

He decided to isolate these jars to observe how the mycelium would duke it out with this mold. 2 of these contaminated jars have shown aggressive rhizomorphic colonization. in fact, I have never seen mycelium tear through a jar or a contamination like this, especially not cubensis. In one of the two jars, the mold is spreading past the ends of the rhizomorphs and is winning. In the other, the mycelium seems to be dominating this mold without allowing it to spread further. It's devouring the competition.

The jars that aren't contaminated are not as rhizomorphic and are colonizing at a snails pace compared to the contaminated jars. It makes me want to pull my hair out. Is there any way He could salvage the aggressive colonizer? He would attempt to clone it, but does not have the resources. He has an abundant supply of dried composted hpoo, coir, and gypsum. Is it worth spawning?

The first two pics are from a couple days ago, the last three from right before the time of posting.











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OfflineMAIA
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Re: Aggressive mycelium in contam jars doing great? [Re: Bassbassik]
    #19240909 - 12/07/13 01:16 PM (10 years, 2 months ago)

I wouldn't use those jars even if mycellium wins. That black mold can be a nasty shit. You can try creating an outdoor bed and see if it survives. Discard any contaminated when you crumble them.


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Offlinesolumvita
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Re: Aggressive mycelium in contam jars doing great? [Re: MAIA]
    #19241342 - 12/07/13 02:50 PM (10 years, 2 months ago)

you have some nice bacteria and yeast in those jars too, discard them!  the mycelium might look fine but will have some bacteria along for the ride


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OfflineBassbassik
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Re: Aggressive mycelium in contam jars doing great? [Re: solumvita]
    #19248903 - 12/09/13 06:25 AM (10 years, 2 months ago)

I was plagued with a 90% contam rate this time around. The other jars exploded with yeast after I posted this. Apparently I screwed up somewhere, or maybe dirty MS. It's a shame because this mycelium looks beastly and I will not get to see it fruit.

How exactly can you tell that there's bacteria in those pics? I didn't notice it and I would like to know for future reference.


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Offline1down5up
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Re: Aggressive mycelium in contam jars doing great? [Re: Bassbassik]
    #19250279 - 12/09/13 01:56 PM (10 years, 2 months ago)

I think the dark spot is a dead giveaway


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OfflineBassbassik
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Re: Aggressive mycelium in contam jars doing great? [Re: 1down5up]
    #19269255 - 12/13/13 03:22 AM (10 years, 2 months ago)

Well yeah...That's the mold.

I was wondering how he can spot bacteria and yeast in the jar, because I missed it.


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Offline1down5up
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Re: Aggressive mycelium in contam jars doing great? [Re: Bassbassik]
    #19269507 - 12/13/13 06:28 AM (10 years, 2 months ago)

bacteria in mine looked sludgy and brownish....and was right next to the glass, so it was easy to see.  I believe it has a distinct smell too...


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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