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blueconfusion
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Just say NO to LC and YES to PDYA
#19249080 - 12/09/13 08:00 AM (10 years, 1 month ago) |
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I'm making about 2000 mL. of PDYA (Potato Dextrose Yeast Agar), but I'll break it down to 500 mL. measurements I just like to make a lot at once fill the Pressure Cooker and always have some on hand. 
WHAT YOU'LL NEED AGAR (order this online, find it in the local health food shop or asian market) POTATO (I just use Russets, I know some people use potato flakes or potato starch) DEXTROSE (this is corn sugar, i know there are substitutions such as karo syrup or honey, but if you have a local brewer's shop it's easy to find) NUTRITIONAL YEAST[OPTIONAL] (this can also usually be found in a health food store i actually found mine in the health food isle at Kroger)
INSTRUCTIONS Boil about 500 mL. (add a little more to allow for evaporation) per 150 grams of peeled and diced potatoes for about 30 minutes.  Then strain the potato water through a cheese cloth or fine mesh strainer.
Next simmer 9 grams of Dextrose and 1 gram of yeast in the water until they dissolve in the potato water. Simmer on low and stir occasionally i never really let it boil i just let it go on low for longer.
Turn off the heat off and slowly add 9 grams of agar stirring until it dissolves. The agar will try to clump so be sure to add it very slowly and stir little bits at a time.
Then pour the PDYA into the receiving vessel. (I use Snapple bottles with a 1/4" hole drilled in the lid and poly-fill stuffed tightly in the hole; to stuff the poly i take a cotton ball sized piece of poly fold it in half twist it and stuff it in with a stick and then cut off the excess with a pair of scissors).


 to avoid a mess use a funnel, and for Snapple bottles I generally pour about 250 mL. per jar just to keep an even and consistent pour and so i don't have to worry about the agar boiling over in the jar.
 Cover the lid tightly with aluminum foil and place in the pressure cooker for 45 minutes @ 15 psi (follow your pressure cooker instructions for pressure cooking).

 what i do with the foil is tear about a 3" strip rip that in half and fold into quarters, i place over the lid and try to tuck it in underneath the lid. Allow your pressure cooker to drop pressure naturally and allow the bottles to cool slightly around 160 degrees (this is just an estimate) and either pour your dishes or allow to fully cool and store in the fridge.
 If you are pouring right way prep your SAB with all your dishes, agar bottles and parafilm or saran wrap. Allow the air to settle in your SAB for at least an hour. Be sure to swirl NOT SHAKE your jars when you pull them out of the pressure cooker as the ingredients may have settle continue to do this lightly swirling in a circular motion until they are cool enough to put in your SAB and pour.
 If you are reheating on the stove put in a stock pot or pressure cooker with something on the bottom to not burn the agar, and i cook on a low-med setting and allow the hot water bath to evenly reheat the jars. Same thing as above be sure to swirl your jars!
 Open your petri dish sleeve in the SAB and stack the dishes Now working smoothly pour your dishes with about a 1/4" of agar working from top to bottom. Allow the dishes to cool overnight in the SAB. Now inoculate with a piece of colonized grain, a piece of clone tissue, a drop of spore solution, or print. Wrap with parafilm or saran wrap and label with date and species.
 IT'S REALLY THAT EASY!!! the extra poured dishes that i won't use go into a gallon ziploc baggy with date and label in the fridge

Edited by blueconfusion (12/13/13 02:28 PM)
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TiN 42
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19249110 - 12/09/13 08:13 AM (10 years, 1 month ago) |
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Sounds good I'll have to try. you don't PC the dishes ?
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: TiN 42]
#19249116 - 12/09/13 08:15 AM (10 years, 1 month ago) |
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no i use plastic disposable ones, i just leave them in the sleeve until right before I pour, I open them in the SAB.
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TiN 42
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19249120 - 12/09/13 08:17 AM (10 years, 1 month ago) |
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Right on thanks.
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: TiN 42]
#19249175 - 12/09/13 08:50 AM (10 years, 1 month ago) |
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I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19249194 - 12/09/13 08:56 AM (10 years, 1 month ago) |
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I haven't been doing agar long but i have seen a little better growth outta the PDYA. I haven't actually done a side by side of PDA and PDYA, but the growth seems IMO to be more aggressive.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19249366 - 12/09/13 10:04 AM (10 years, 1 month ago) |
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i actually ended up with about 1750 mL of agar going into jars, i started with about 2300 mL. of water boiling the potatoes.
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Pestile

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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19249670 - 12/09/13 11:29 AM (10 years, 1 month ago) |
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Where do you store the excess media bottles and how do you heat them up again?
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19249723 - 12/09/13 11:43 AM (10 years, 1 month ago) |
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I used to use Snapple bottles too. They work great.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pestile]
#19249784 - 12/09/13 11:57 AM (10 years, 1 month ago) |
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Quote:
Pestilence said: Where do you store the excess media bottles and how do you heat them up again?
I usually just keep the foil on them put them in gallon ziploc baggies and throw them in the fridge. I haven't had any problems with this yet. To reheat them I use my PC with the tray on the bottom and fill it with water to about half way up the jars, i don't bring it to a boil i'm just looking to liquefy not recook the agar.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19251172 - 12/09/13 04:51 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: I used to use Snapple bottles too. They work great.
i don't think i had ever seen your write up Jedi, or if i had it was when i was still scared to do agar, what a fool i was agar is so easy!
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19251185 - 12/09/13 04:54 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said: I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?
i use pda also its just add water and pc and pour. but from my understanding myc likes when you switch up the mix after a few transfers or somehting of that nature.
i havent ran into this problem yet so i havent looked into to far
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19251188 - 12/09/13 04:55 PM (10 years, 1 month ago) |
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also that is a shit TON of agar how many dishes you gonna make son
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19251201 - 12/09/13 04:58 PM (10 years, 1 month ago) |
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Quote:
twistedty said: also that is a shit TON of agar how many dishes you gonna make son 
well i have a few friends sending me some prints, plus i have my own isolates i'm working on so... as many as i can!
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19251671 - 12/09/13 06:20 PM (10 years, 1 month ago) |
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Quote:
twistedty said:
Quote:
Pastywhyte said: I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?
i use pda also its just add water and pc and pour. but from my understanding myc likes when you switch up the mix after a few transfers or something of that nature.
i haven't ran into this problem yet so i havent looked into to far
Yeah I use MEA for my slants and for my plates I try to rotate PDA, grain soak water, and dog food. Been reading fastfreds cookbook a lot lately and thinkin bout throwing a few new recipes into the mix. Gonna do some MEA pour plates up soon (got a few sleeves o the X ones) with a little tetracycline, got a lot of wild prints to attend to. Shit I'm seeing agar potential everywhere these days. Poured myself a bowl of rice krispies and thought wait a sec. . .
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19251687 - 12/09/13 06:24 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said:
Quote:
twistedty said:
Quote:
Pastywhyte said: I have used a lot of PDA in the past with little issue. How much benefit would you say PDYA has over simple PDA?
i use pda also its just add water and pc and pour. but from my understanding myc likes when you switch up the mix after a few transfers or something of that nature.
i haven't ran into this problem yet so i havent looked into to far
Yeah I use MEA for my slants and for my plates I try to rotate PDA, grain soak water, and dog food. Been reading fastfreds cookbook a lot lately and thinkin bout throwing a few new recipes into the mix. Gonna do some MEA pour plates up soon (got a few sleeves o the X ones) with a little tetracycline, got a lot of wild prints to attend to. Shit I'm seeing agar potential everywhere these days. Poured myself a bowl of rice krispies and thought wait a sec. . . 
ya myc loves to thrive, there is pic somewhere round here where a mushroom fruited in a syringe and started coming out of the needle.
apparently the dude forgot about it for along time.
ive seen em growing out of a garbage disposal in a sink also on another site.
mycelium just doesnt give a fuck
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19251697 - 12/09/13 06:25 PM (10 years, 1 month ago) |
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19251733 - 12/09/13 06:30 PM (10 years, 1 month ago) |
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the very first time i tried agar was the BRF substitute no pour in 1/4 pint jelly jars that was about a year ago i kinda gave up for a while because of my limited success there. this time i made PDA and MEA, but then FH encouraged me to add the yeast so i did and it really does work great. Might try a MYA soon as well. I have also been reading through a lot of the cookbooks, you can mix about anything and mycelium will grow.
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19251741 - 12/09/13 06:31 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: the very first time i tried agar was the BRF substitute no pour in 1/4 pint jelly jars that was about a year ago i kinda gave up for a while because of my limited success there. this time i made PDA and MEA, but then FH encouraged me to add the yeast so i did and it really does work great. Might try a MYA soon as well. I have also been reading through a lot of the cookbooks, you can mix about anything and mycelium will grow.
my point exactly i mean, dog food grain water etc. myc is amazing
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19251747 - 12/09/13 06:32 PM (10 years, 1 month ago) |
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hey pasty how much tetracycline you add? i have some wild prints on the way
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19251753 - 12/09/13 06:33 PM (10 years, 1 month ago) |
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and how and when do you mix it in i know some antibiotics are heat sensitive
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19251766 - 12/09/13 06:35 PM (10 years, 1 month ago) |
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This will be my first go with it so I'm just doing research now. Something tells me that a little goes a long way. Think about the size of most peoples fish tanks, then look at the plate. I do know that it cannot be PC'd so it needs to be added as the agar is cooling and gently swirled in.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19251796 - 12/09/13 06:38 PM (10 years, 1 month ago) |
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really want to know the results i may have to conduct my own experiments this weekend i have a Snapple jar just sitting waiting to be used and some used dishes that are just calling to be my test monkeys  i had tried with some other antibiotic a couple weeks ago but totally messed it up and i don't think it was the right stuff saw tetracycline almost grabbed it for round two but i wanted to research and see if anyone else had done it, and how they went about it. what better time then here and now to find out
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19251846 - 12/09/13 06:45 PM (10 years, 1 month ago) |
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After autoclaving, and when the agar has cooled enough that it’s not too hot to touch (about 1 to 1.5hrs), add antibiotics as follows: Ampicillin – add 1ml ampicillin (at 100mg/ml) per liter of agar to obtain a final concentration of 100ug/ml. Mark the plate with a single red line on the side. Kanamycin – add 1ml kanamycin stock (at 50mg/ml) per liter of agar to obtain a final concentration of 50ug/ml. Mark the plates with a single green line on the side. Tetracycline – add 1ml tetracycline stock (at 15mg/ml) per liter of agar to obtain a final concentration of 15ug/ml. Mark the plates with a single black line on the side. Chloramphenicol – add 1ml chloramphenicol stock (at 25mg/ml) per liter of agar to obtain a final concentration of 100ug/ml. Mark the plates with a single purple line on the side.
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19251967 - 12/09/13 07:08 PM (10 years, 1 month ago) |
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holy shit dude all that trouble, when you just need to add some water to a fully colonized grain jar, shake and extract the juice enough to inoculate 100's of jars, GLC ftw
simple and fool proof
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19251977 - 12/09/13 07:10 PM (10 years, 1 month ago) |
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Simple, but prone to contamination.
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19251985 - 12/09/13 07:11 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: After autoclaving, and when the agar has cooled enough that it’s not too hot to touch (about 1 to 1.5hrs), add antibiotics as follows: Ampicillin – add 1ml ampicillin (at 100mg/ml) per liter of agar to obtain a final concentration of 100ug/ml. Mark the plate with a single red line on the side. Kanamycin – add 1ml kanamycin stock (at 50mg/ml) per liter of agar to obtain a final concentration of 50ug/ml. Mark the plates with a single green line on the side. Tetracycline – add 1ml tetracycline stock (at 15mg/ml) per liter of agar to obtain a final concentration of 15ug/ml. Mark the plates with a single black line on the side. Chloramphenicol – add 1ml chloramphenicol stock (at 25mg/ml) per liter of agar to obtain a final concentration of 100ug/ml. Mark the plates with a single purple line on the side.
Good stuff
Quote:
loki44 said: holy shit dude all that trouble, when you just need to add some water to a fully colonized grain jar, shake and extract the juice enough to inoculate 100's of jars, GLC ftw
simple and fool proof
Well I am working with wild spores and clones and many of them are not as fast as cubensis. So antibiotic is good. As for regular PDA or PDYA its as easy as making jello A GLC is not guaranteed either. You could have just inoculated 100's of jars with mold. Agar FTW.
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PirateSwazey



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252002 - 12/09/13 07:14 PM (10 years, 1 month ago) |
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Hey blue,
What's your motivation for using the snapple bottles vs a liquor bottle?
Just to have a pre-measured amount of agar for your petri?
Nice write up
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19252029 - 12/09/13 07:19 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: Simple, but prone to contamination.
no, key word here FULLY colonized, the problem is people get impatient and go ahead without letting the mycelium take over 100% of the grains and beyond, when you see all the grains white as snow and the mycelium crawling all over the glass because there's no more grain that's when is ready, not before
you don't really need all that trouble and expense if you are using MS, now if you want isolates agar is a must but for most people who are not in the comercial side but just wanna do a couple monos here and there GLC is the way to go
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: loki44] 1
#19252059 - 12/09/13 07:27 PM (10 years, 1 month ago) |
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Your grains are not going to be 100% sterile. The dormant spores or endospores will eventually germinate. It doesn't matter if your spawn is 100% colonized, it will still happen.
The subject has been debated many times. People with experience have mostly abandoned them and moved on to agar, which is what this thread is about AND the originator GLC has said he wished he'd never wrote the tek.
This is not my thread so, I will not say anything more. But, telling these guys on this thread to use GLC will get you nowhere because they already know the deal.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
Edited by SpitballJedi (12/09/13 07:30 PM)
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19252074 - 12/09/13 07:30 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Well I am working with wild spores and clones and many of them are not as fast as cubensis. So antibiotic is good. As for regular PDA or PDYA its as easy as making jello A GLC is not guaranteed either. You could have just inoculated 100's of jars with mold. Agar FTW.
OP what other species are you working with?well if you are cloning and using wild spores you need dat dere agar
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252075 - 12/09/13 07:30 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
SpitballJedi said: Simple, but prone to contamination.
no, key word here FULLY colonized, the problem is people get impatient and go ahead without letting the mycelium take over 100% of the grains and beyond, when you see all the grains white as snow and the mycelium crawling all over the glass because there's no more grain that's when is ready, not before
you don't really need all that trouble and expense if you are using MS, now if you want isolates agar is a must but for most people who are not in the comercial side but just wanna do a couple monos here and there GLC is the way to go
So what about the center of the jar, where your ms inoculation deposited some sneaky trich, and you cant see from the side, and when you shake it up the mycelium is white. Your GLC still good? Not to mention spitballs point about the endospores, which I firmly believe in. Agar is not hard, and you don't need to be a "commercial" grower to enjoy it on a small scale. Frankly I find agar to be one of the most enjoyable aspects of the whole grow and I would probably have been bored already if it didn't exist.
Edited by Pastywhyte (12/09/13 07:39 PM)
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252091 - 12/09/13 07:36 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
blueconfusion said:
Well I am working with wild spores and clones and many of them are not as fast as cubensis. So antibiotic is good. As for regular PDA or PDYA its as easy as making jello A GLC is not guaranteed either. You could have just inoculated 100's of jars with mold. Agar FTW.
OP what other species are you working with?well if you are cloning and using wild spores you need dat dere agar
That was actually my quote. If you must know I'm working on some wild Aussie cubes, some p alleni, wild subs collected from several parts of the world, a wild print of hericium coralloides, I'm also gonna get a buddy to let me swab the gills of his wild reishi that he found, ps cyancesens, and a few others in the mix as well.
Edited by Pastywhyte (12/09/13 07:45 PM)
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kechlesurf
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252112 - 12/09/13 07:39 PM (10 years, 1 month ago) |
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Sounds fun. Liquid culture is a pain in the ass
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: kechlesurf]
#19252128 - 12/09/13 07:43 PM (10 years, 1 month ago) |
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It is but I am starting to work with it for the sake of my mexicana and pan experiments. Still use a wedge to inoculate it of course.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: PirateSwazey]
#19252134 - 12/09/13 07:45 PM (10 years, 1 month ago) |
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Quote:
PirateSwazey said: Hey blue,
What's your motivation for using the snapple bottles vs a liquor bottle?
Just to have a pre-measured amount of agar for your petri?
Nice write up 
Liquor bottles don't fit in my PC and thank you
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19252151 - 12/09/13 07:48 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: Your grains are not going to be 100% sterile. The dormant spores or endospores will eventually germinate. It doesn't matter if your spawn is 100% colonized, it will still happen.
The subject has been debated many times. People with experience have mostly abandoned them and moved on to agar, which is what this thread is about AND the originator GLC has said he wished he'd never wrote the tek.
This is not my thread so, I will not say anything more. But, telling these guys on this thread to use GLC will get you nowhere because they already know the deal.
Do you realize that those dormant spores or whatever nasties are gonna still be in your grain when you spawn to bulk? I'm not trying to convince anyone. Most experienced growers in this site grow at fairly large scale and it would be a disaster to lose an entire crop because of bad spawn so agar is understandable in their particular circumstances. Once you have more to lose, makes sense to go the extra step to avoid contamination, but just because they do it doesn't mean is necessary for a successful grow
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252174 - 12/09/13 07:51 PM (10 years, 1 month ago) |
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i will also be getting some wild spores, this was my interest in the antibiotic agar, other than that i prefer agar for all the points made by spitball and pasty. i am no where near a commercial grower i do this for fun and am simply trying to provide the info to those new growers that think an LC or GLC is the way to go. I have done both and both have contaminated on me. if my agar is contaminated i don't have to throw the whole thing away i can simply isolate good tissue away from the contamination easy as that! how you gonna save a GLC or LC?
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252187 - 12/09/13 07:53 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
SpitballJedi said: Your grains are not going to be 100% sterile. The dormant spores or endospores will eventually germinate. It doesn't matter if your spawn is 100% colonized, it will still happen.
The subject has been debated many times. People with experience have mostly abandoned them and moved on to agar, which is what this thread is about AND the originator GLC has said he wished he'd never wrote the tek.
This is not my thread so, I will not say anything more. But, telling these guys on this thread to use GLC will get you nowhere because they already know the deal.
Do you realize that those dormant spores or whatever nasties are gonna still be in your grain when you spawn to bulk? I'm not trying to convince anyone. Most experienced growers in this site grow at fairly large scale and it would be a disaster to lose an entire crop because of bad spawn so agar is understandable in their particular circumstances. Once you have more to lose, makes sense to go the extra step to avoid contamination, but just because they do it doesn't mean is necessary for a successful grow
yes but there is a difference between spawning to a properly pasteurized bulk with dormant spores than propagating those dormant spores by moving them to a sterilized grain
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252195 - 12/09/13 07:55 PM (10 years, 1 month ago) |
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Quote:
loki44 said: Most experienced growers in this site grow at fairly large scale and it would be a disaster to lose an entire crop because of bad spawn so agar is understandable in their particular circumstances. Once you have more to lose, makes sense to go the extra step to avoid contamination, but just because they do it doesn't mean is necessary for a successful grow
Most of them also grow more than one variety/species at a time as well so they would need different cultures anyway. Agar just makes sense for them, however I would say for the average cultivator who just wants a tub of good shrooms once a year agar is just as worthwhile. That way he can have a good culture slanted up and when he is ready he can just grow it without having to worry about things like, poor yield, or bunk potency or all the other crap that you can end up with doing ms. Oh and his one grow a year is probably pretty important to him so he got just as much to lose if it ends up in the toilet cause he did a GLC.
Really if I have no choice but to do an LC, I would take an LC inoculated with a clean agar wedge over a GLC any day
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19252224 - 12/09/13 07:59 PM (10 years, 1 month ago) |
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GLC's are outdated if you really don't want to do agar but expand your MS why not do a G2G? at least this way you can open up your fully colonized jar and smell it. and then make that one jar into 14... that would be 2 tubs and be more than enough for anyone looking for just a head stash in the neighborhood of around 8-16 oz.
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252269 - 12/09/13 08:06 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: GLC's are outdated if you really don't want to do agar but expand your MS why not do a G2G? at least this way you can open up your fully colonized jar and smell it. and then make that one jar into 14... that would be 2 tubs and be more than enough for anyone looking for just a head stash in the neighborhood of around 8-16 oz.
GLC can speed this up, if you shoot lets say 10cc's per jar, jars will be ready prbly in a week after inoculation, also if you are planing to do brf cakes you cant g2g
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252311 - 12/09/13 08:14 PM (10 years, 1 month ago) |
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but you can still do agar. That's what I do to test cultures.
A contaminated GLC will stop yu in your tracks, ruin a bunch of work and materials, waste your time, and is more likely to happen the longer you keep the GLC
And, like was already said, a grain jar can be seriously contaminated and you won't know it till it's too late.
Quote:
Pastywhyte said: I would take an LC inoculated with a clean agar wedge over a GLC any day 
 I honestly can't think of a single reason to ever do GLC again.
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Edited by SpitballJedi (12/09/13 08:15 PM)
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252317 - 12/09/13 08:15 PM (10 years, 1 month ago) |
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my g2g are done in a week tops no you can't g2g brf but you can use a clean agar wedge shoot some water onto the clean mycelium and use a clean needle to make a clean syringe same as your GLC but i can at least sleep at night knowing its clean or i can use that clean agar wedge to make an LC that i know is clean
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252343 - 12/09/13 08:21 PM (10 years, 1 month ago) |
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found a pic of loki
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252380 - 12/09/13 08:28 PM (10 years, 1 month ago) |
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I just put the agar wedge right on top of the cake when I do tests
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
Edited by SpitballJedi (12/09/13 08:30 PM)
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252404 - 12/09/13 08:33 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
found a pic of loki 
LOL why you people can't understand that someone may want to keep it simple and effective, you are gonna tell me that buying a ton of extra supplies, investing time in learning new skills, occupying extra space, in other words more shit to accomplish the same goal is the best way, are you kidding me?
if all you you need 14/21 jars for 2 or 3 tubs max, you really dont need , an unlimited supply of petri dishes,parafilm, inoc loop, agar and all the other shit, extra refrigeration space etc etc etc, come on guy have some common sense here
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252419 - 12/09/13 08:36 PM (10 years, 1 month ago) |
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Quote:
loki44 said: holy shit dude all that trouble, when you just need to add some water to a fully colonized grain jar, shake and extract the juice enough to inoculate 100's of jars, GLC ftw
simple and fool proof
terrible idea, agar is just as easy.
hes just talkin about advanced techniques for agar for wilds
GLC is worse than LC lol
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252425 - 12/09/13 08:37 PM (10 years, 1 month ago) |
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Quote:
loki44 said: LOL why you people can't understand that someone may want to keep it simple and effective, you are gonna tell me that buying a ton of extra supplies, investing time in learning new skills, occupying extra space, in other words more shit to accomplish the same goal is the best way, are you kidding me?
Yeah I forgot, most people are here for drugs Wouldn't want to learn anything or do something with skill. Agar is not hard and can be done for very cheap with supplies bought from the grocery store. Why not spend $20 bucks extra to avoid wasting everything
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252427 - 12/09/13 08:37 PM (10 years, 1 month ago) |
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If all you need is 14 to 21 jars you don't need to do an LC
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252447 - 12/09/13 08:41 PM (10 years, 1 month ago) |
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You don't have to use petri dishes you can use 1/4 pint jelly jars or ziploc tupperware dishes you don't need parafilm you can use saran wrap agar is cheap there are a lot of agar recipes out there
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252454 - 12/09/13 08:43 PM (10 years, 1 month ago) |
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im glad someone else is with me like hacker, frank, blue, and pasty to start the agar movement. i did my first batch of agar for 5 bux out of dog food from my neighbor and some agar agar from the health store and some no pour jars.
fell in love.
bought big ass roll of parafilm, a case of 500 dishes and 1000 grams of pda.
my success rate right now since i started proper pastuerization and agar and my slants on isolates is at least 90% probably way higher but ill be modest for now.
and LCs cant do this

my isolate library which can be stored for years if i decide to take a break
its a no brainer i dont understand why people would even try to argue.
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252456 - 12/09/13 08:43 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: You don't have to use petri dishes you can use 1/4 pint jelly jars or ziploc tupperware dishes you don't need parafilm you can use saran wrap agar is cheap there are a lot of agar recipes out there
yes very very true. but i have money and when i do something i go all out bro
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19252457 - 12/09/13 08:43 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said:
 I honestly can't think of a single reason to ever do GLC again.
duh, for whatever reason you decided to invest all the resources necessary to do agar work, why would you do LC's now? the point is for some small scale growers AGAR is NOT necessary to successfully grow
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252465 - 12/09/13 08:44 PM (10 years, 1 month ago) |
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And loki the new skill you talk of is as easy as making jello so can you boil water?
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252472 - 12/09/13 08:45 PM (10 years, 1 month ago) |
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Quote:
twistedty said:

Nice slants, I just got the plastic ones and vacutainers. But we got the same rack
Edited by Pastywhyte (12/09/13 08:51 PM)
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252477 - 12/09/13 08:47 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
SpitballJedi said:
 I honestly can't think of a single reason to ever do GLC again.
duh, for whatever reason you decided to invest all the resources necessary to do agar work, why would you do LC's now? the point is for some small scale growers AGAR is NOT necessary to successfully grow
For the small scale grower there is no point to do GLC's or LC's just do G2G in your own words DUH!!!
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252487 - 12/09/13 08:49 PM (10 years, 1 month ago) |
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Nice write up
I think i need to get into agar next paycheck im gonna get some supplies.
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252495 - 12/09/13 08:50 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
SpitballJedi said:
 I honestly can't think of a single reason to ever do GLC again.
duh, for whatever reason you decided to invest all the resources necessary to do agar work, why would you do LC's now? the point is for some small scale growers AGAR is NOT necessary to successfully grow
doesnt matter what scale grow youre doing, its the success we are after. and you can make isolates and clones?
like i said its a no brainer no matter if youre doing 20-30 tub grows like a few of us or just 2 tubs.
like i said since ive switched to agar alonnng time ago my success and yields/quality has shot threw the roof
lol GLCs. i would rather do an MS syringe. oh wait i dont use syringes anymore :/
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252504 - 12/09/13 08:51 PM (10 years, 1 month ago) |
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So why are you still here loki
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252506 - 12/09/13 08:52 PM (10 years, 1 month ago) |
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My opinion is based on personal experience. The investment in to agar came after LC and GLC.
If you want to make GLC and find it beneficial, then do it.
There is nothing you can say about it that hasn't already been said a million times. We already know all the arguments. You are simply barking up the wrong tree by trying to convince anyone on this thread.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252507 - 12/09/13 08:52 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: And loki the new skill you talk of is as easy as making jello so can you boil water?
thats my line btw 
the first time i did my PDA i was like wow. soooo easy couldnt believe it. especially after seeing all the pics of people doing LCs with all their syringes and magnets in their jars with their fancy magnetic stirrers they built and nocc ports. and then the waiting game and then omg my LC looks awesome.
3 weeks later omg all my jars are failed etc.
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19252512 - 12/09/13 08:53 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: My opinion is based on personal experience. The investment in to agar came after LC and GLC.
If you want to make GLC and find it beneficial, then do it.
There is nothing you can say about it that hasn't already been said a million times. We already know all the arguments. You are simply barking up the wrong tree by trying to convince anyone on this thread.
QFT. youre like a caveman way behind the curve. no matter how many mushrooms you grow or not
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252519 - 12/09/13 08:55 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
For the small scale grower there is no point to do GLC's or LC's just do G2G in your own words DUH!!!
you cant g2g on brf jars! damn it lol I wanna do grains and pf-tek, is just easier and faster to shoot everything with a nice glc and you are good to go, brf hars are done in 2 weeks grain jars are 90% done without even shaking
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252531 - 12/09/13 08:56 PM (10 years, 1 month ago) |
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Quote:
twistedty said:
Quote:
blueconfusion said: And loki the new skill you talk of is as easy as making jello so can you boil water?
thats my line btw 
Sorry
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252539 - 12/09/13 08:58 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
blueconfusion said:
For the small scale grower there is no point to do GLC's or LC's just do G2G in your own words DUH!!!
you cant g2g on brf jars! damn it lol I wanna do grains and pf-tek, is just easier and faster to shoot everything with a nice glc and you are good to go, brf hars are done in 2 weeks grain jars are 90% done without even shaking

a nice GLC is an oxymoron.
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252545 - 12/09/13 08:59 PM (10 years, 1 month ago) |
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I think violet did a brf with agar wedges didn't she? No verm layer just threw the wedge in and let it go may have been rgs but could do same thing
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252551 - 12/09/13 09:00 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Quote:
twistedty said:
Quote:
blueconfusion said: And loki the new skill you talk of is as easy as making jello so can you boil water?
thats my line btw 
Sorry 
no i want you spread the word brother, its the best analogy i could come up with. and it truly is.
or we can make some awesome sticky syrup LCs
and then this

then wait for it to colonize then knock up some test jars and wait and then hope its good and we dont have to start over
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19252561 - 12/09/13 09:02 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: cial, then do it.
There is nothing you can say about it that hasn't already been said a million times. We already know all the arguments. You are simply barking up the wrong tree by trying to convince anyone on this thread.
why would I want to convince anyone to do anything here lol. I'm just expressing my opinion since the noob perspective, which most people in this thread are not btw. Noobs dont need to be shove down their throat the "agar dogma" they can do fine with GLC,agar is great but reading some of you guys is like oh noez you do GLC and your grow will explode
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252574 - 12/09/13 09:05 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
why would I want to convince anyone to do anything here lol. I'm just expressing my opinion since the noob perspective, which most people in this thread are not btw. Noobs dont need to be shove down their throat the "agar dogma" they can do fine with GLC,agar is great but reading some of you guys is like oh noez you do GLC and your grow will explode 
lolllllllllllllllll. im done :/
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252581 - 12/09/13 09:06 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
SpitballJedi said: cial, then do it.
There is nothing you can say about it that hasn't already been said a million times. We already know all the arguments. You are simply barking up the wrong tree by trying to convince anyone on this thread.
why would I want to convince anyone to do anything here lol. I'm just expressing my opinion since the noob perspective, which most people in this thread are not btw. Noobs dont need to be shove down their throat the "agar dogma" they can do fine with GLC,agar is great but reading some of you guys is like oh noez you do GLC and your grow will explode 
It may explode with trich I know mine did!
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252593 - 12/09/13 09:10 PM (10 years, 1 month ago) |
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And I'm not shoving anything down anyone's throat I'm just providing information you were the one that came on to my thread and started running your mouth.
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252622 - 12/09/13 09:16 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: Being thati Quote:
loki44 said:
Quote:
SpitballJedi said: cial, then do it.
There is nothing you can say about it that hasn't already been said a million times. We already know all the arguments. You are simply barking up the wrong tree by trying to convince anyone on this thread.
why would I want to convince anyone to do anything here lol. I'm just expressing my opinion since the noob perspective, which most people in this thread are not btw. Noobs dont need to be shove down their throat the "agar dogma" they can do fine with GLC,agar is great but reading some of you guys is like oh noez you do GLC and your grow will explode 
It may explode with trich I know mine did!
you keep using your GLC, ill stick with my clean cultures/isolates. use your nasty MS grain water. thats whats seperates the men from the boys.
we just want everyone on here to have the utmost cleanest success with the least amount of steps to do so.
and agar is the way. take clean culture cut with sterilized blade and drop in jar.
syringes should be only used one time. and that is to nocc up your first jars.
after that you should take a print and throw it on agar.
i dont even know why im still arguing with you. maybe hes just trollin us
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252640 - 12/09/13 09:20 PM (10 years, 1 month ago) |
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Shit I only use one drop outta my syringes. Got about half a dozen in the fridge with one drop missing. Never needed to go back to em. Buy a variety once, have it forever.
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252656 - 12/09/13 09:22 PM (10 years, 1 month ago) |
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https://www.shroomery.org/forums/showflat.php/Number/19228936#19228936
Quote:
loki44 said: I have done 2 pf-tek grows
https://www.shroomery.org/forums/showflat.php/Number/19140900#19140900
Quote:
loki44 said: hi guys, this is my first ever grain jar
You don't have enough experience to have a valid opinion. Your lack of experience is why you think the way you do.
It's bad form to come on someones thread to criticize when your lack of experience means you are talking complete shit.
You can't establish a reliable pattern for success or growth rate in 2 PF grows and 1 spawn run
Quote:
blueconfusion said: And I'm not shoving anything down anyone's throat I'm just providing information you were the one that came on to my thread and started running your mouth.
Exactly
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19252658 - 12/09/13 09:22 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said: Shit I only use one drop outta my syringes. Got about half a dozen in the fridge with one drop missing. Never needed to go back to em. Buy a variety once, have it forever.
same here.
hey loki call me when your isolates do this 152grams

oh wait you cant make jello
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252672 - 12/09/13 09:25 PM (10 years, 1 month ago) |
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sorry i feel like im coming off as a dick, im just very adamant about people on here having as much success as i am.
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252680 - 12/09/13 09:26 PM (10 years, 1 month ago) |
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I like dick's. Wait, that came out wrong.
Fuck'im. He's trollin.
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252681 - 12/09/13 09:27 PM (10 years, 1 month ago) |
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Quote:
twistedty said:
you keep using your GLC, ill stick with my clean cultures/isolates. use your nasty MS grain water. thats whats seperates the men from the boys.
we just want everyone on here to have the utmost cleanest success with the least amount of steps to do so.
haha dude , dont get your panties in a bunch,less steps than GLC? how? grain+water+shake=GLC come on you cant beat that "dirty water tek" 
the men from the boys? ok
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252738 - 12/09/13 09:34 PM (10 years, 1 month ago) |
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sorry just do your thing man
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19252754 - 12/09/13 09:36 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: https://www.shroomery.org/forums/showflat.php/Number/19228936#19228936
Quote:
loki44 said: I have done 2 pf-tek grows
https://www.shroomery.org/forums/showflat.php/Number/19140900#19140900
Quote:
loki44 said: hi guys, this is my first ever grain jar
You don't have enough experience to have a valid opinion. Your lack of experience is why you think the way you do.
It's bad form to come on someones thread to criticize when your lack of experience means you are talking complete shit.
You can't establish a reliable pattern for success or growth rate in 2 PF grows and 1 spawn run
Quote:
blueconfusion said: And I'm not shoving anything down anyone's throat I'm just providing information you were the one that came on to my thread and started running your mouth.
Exactly
if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no
why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil not addressing your post particularly but the tone of several posters
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19252776 - 12/09/13 09:40 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: And I'm not shoving anything down anyone's throat I'm just providing information you were the one that came on to my thread and started running your mouth.
all the info you provided is awesome, you are a great poster. I just felt I needed to say something about the "say NO to LC" not trying to take anything about your agar procedure, but I dont hink theres need to shit on another tek, my 2 cents
Edited by loki44 (12/09/13 09:41 PM)
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252780 - 12/09/13 09:41 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Exactly
if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no
why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil not addressing your post particularly but the tone of several posters
agreed. i pulled off the bucket tek 10x with 0 problems when i was a newb. then i was like well im gonna do 20 tubs.
guess what 15 tubs got trich .
like i said we want everyone on here to have utmost success and teach others the same methods.
proper pasteurization is #1 and then agar is tied.
no hard feeling bro, if i came to your house id definetly eat your glc boomers with no complaints
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252802 - 12/09/13 09:45 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
all the info you provided is awesome, you are a great poster. I just felt I needed to say something about the "say NO to LC" not trying to take anything about your agar procedure, but I dont hink theres need to shit on another tek, my 2 cents
the bucket tek works too. any EXPERIENCED grower will not reccomend it either.
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252847 - 12/09/13 09:52 PM (10 years, 1 month ago) |
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Quote:
twistedty said:
Quote:
loki44 said:
Exactly
if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no
why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil not addressing your post particularly but the tone of several posters
agreed. i pulled off the bucket tek 10x with 0 problems when i was a newb. then i was like well im gonna do 20 tubs.
guess what 15 tubs got trich .
like i said we want everyone on here to have utmost success and teach others the same methods.
proper pasteurization is #1 and then agar is tied.
no hard feeling bro, if i came to your house id definetly eat your glc boomers with no complaints 
haha,no hard feeligs either, but I cant bash something I have had no contams, only when a PATTERN of contamination arises then you can say ok, there is a problem, don't be too quick to blame your spawn, could be you didn't properly pasteurized, or your substrate wasn't at field capacity etc not talking about you in particular, just generally speaking
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252855 - 12/09/13 09:54 PM (10 years, 1 month ago) |
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Quote:
loki44 said: if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no
why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil not addressing your post particularly but the tone of several posters
Keep doing it. But please make a new thread with your results over time.
It's hard to say why shitty practices work sometimes. In my line of work, I'm amazed at the things that work sometimes when they really shouldn't be because usually they don't.
Many people have made successful GLC. But don't get dependent on it because it's unreliable. You can't see this yet because you have not been doing it enough.
Some people are dogmatic about stuff and get upset when you disagree. Nobody's butthurt here, I just think you're being silly. There is a difference between dogma and real experience.
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19252859 - 12/09/13 09:55 PM (10 years, 1 month ago) |
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nah my spawn is on point . bucket tek was the problem fo show
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19252886 - 12/09/13 10:01 PM (10 years, 1 month ago) |
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I found myself blaming the bucket for some of my problems, namely verticillium which I saw more than trich for sure. As verticillium is supposed to only be introduced during spawning I figured that was my vector and abandoned the bucket. Since starting proper pasteurization I only saw verticillium once and it was a mild case, but funny enough, I have been starting to sterilize my coir verm and I thought for sure the dry bubble would come back at that point, however I have yet to see it Gonna do a whole shitload more sterilization projects in the future tho just to be sure.
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19252939 - 12/09/13 10:10 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said: I found myself blaming the bucket for some of my problems, namely verticillium which I saw more than trich for sure. As verticillium is supposed to only be introduced during spawning I figured that was my vector and abandoned the bucket. Since starting proper pasteurization I only saw verticillium once and it was a mild case, but funny enough, I have been starting to sterilize my coir verm and I thought for sure the dry bubble would come back at that point, however I have yet to see it Gonna do a whole shitload more sterilization projects in the future tho just to be sure.
youre sterilizing your coir/verm? never had vert myself only trich from the bucket. and a mild green mold from some mold hidden in my agar that would show up during/after first flush but didnt do anything negative to my harvest.
was able to flush it out np but threw it away anyway.
got rid of those cultures and havent had any probs since 
its always good to be able to diagnose the problem.
frank had the same problem as well during the same time, a lil collaboration and he thought it was the agar as well.
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19253022 - 12/09/13 10:32 PM (10 years, 1 month ago) |
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Quote:
twistedty said: youre sterilizing your coir/verm?
Sure am Here is the link. First flush is already in the can and one of the tubs is showing the second already.
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19253027 - 12/09/13 10:34 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said:
Quote:
twistedty said: youre sterilizing your coir/verm?
Sure am Here is the link. First flush is already in the can and one of the tubs is showing the second already.


roadhouse. you autoclave or.....
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19253031 - 12/09/13 10:34 PM (10 years, 1 month ago) |
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nm i clicked the link
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19253040 - 12/09/13 10:37 PM (10 years, 1 month ago) |
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well shit,im ALWAYS in my kitchen my stovetop only holds 2 pcs and thats only 14 qts and i have to do 28 a day damn near for 3 tubs.
its like an 8 hr job.
might give this a shot fo show
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19253046 - 12/09/13 10:39 PM (10 years, 1 month ago) |
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Get in on the action, everyone is welcome I believe Spitball is doing a tub and I think Frank is up for it as well
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19253992 - 12/10/13 05:34 AM (10 years, 1 month ago) |
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I should be putting mine in fruitin in the next day or two
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19254034 - 12/10/13 06:01 AM (10 years, 1 month ago) |
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@Loki i didn't mean for the "Just say NO to LC" to come off as i was bashing any one tek, i had success with my LC; for a while... I was all about LC's for about 4 months 0 problems the same with the bucket tek, the bucket tek is great i've had success with that as well. i work 60 hours a week so the "easy way" was the most appealing for me, until i realized the potential i was missing out on, the actual science of this hobby. i am by no means "a commercial" grower as you have called us, and i don't have lots of money to waste this is why i'm trying to help others who want to know how to make the most of the dollar they spend. i do pf cakes too, but what i do is just a drop on my agar (which is more than enough) and then inoculate some brf cakes; in fact i have 12 cakes fruiting right now. i have also found ways to stretch that syringe out i can actually make somewhere around 24 cakes and a plate or two of agar from one syringe... and i don't need syrup water to do that i just use some distilled water. i shoot a few cakes up and then suck up some distilled water shake and keep going.
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19254081 - 12/10/13 06:24 AM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: I should be putting mine in fruitin in the next day or two
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19254093 - 12/10/13 06:32 AM (10 years, 1 month ago) |
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i have a little extra time today, actually taking a day off work so i might get started on my antibiotic agar again ROUND 2; this time I'll be using MEA just cause I have all the stuff to do it, and i don't wanna boil potatoes ... the mix will most likely be 9 grams of Malt Extract and 9 grams of Agar, in 500 mL. water. As for the tetracycline probably only add maybe a 1/2 tsp to one jar and a full tsp. to the other once they have cooled enough. gonna prep pretty much same way as PDYA simmer ME in 500 mL. of water until it has dissolves remove from heat add agar, then PC let cool for about an hour to 1.5 hours... i'm probably gonna pour this in old plates and use a grain as spawn. i'll post results as i go.
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19254202 - 12/10/13 07:31 AM (10 years, 1 month ago) |
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had just enough agar to do this experiment (antibiotic w/ tetracycline). hoping it turns out just put my vessels in the PC to get this going. after all the debate last night maybe i should have not posted this thread as "just say no to lc and yes to pdya", but rather "can you make jello then try agar", thanks twisted where were you when i was creating this thread i love that quote it's so true! gonna change how much tetracycline i add as well. i'll let everyone know as soon as i get to that point
Edited by blueconfusion (12/10/13 07:37 AM)
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19254385 - 12/10/13 08:49 AM (10 years, 1 month ago) |
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ok the scientific side of this experiment just went out the window i just realized my friend has my scales to measure anything small so i'm eyeballing it (he's outta town for a while) so here is my eyeballing

 if i can get something outta this i'll get some more precise measurements but those are tsps. with about a knife tip of tetracycline on each tsp.
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19254408 - 12/10/13 08:56 AM (10 years, 1 month ago) |
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I fucking love to eyeball shit with a pinch of this and a dash of that Can't wait to see your results. I'm thinking of doing the same thing really. Looking at getting it going tomorrow maybe. Just trying to decide what type of agar to go with. Might do MEA, i haven't made any of that for a while.
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19254438 - 12/10/13 09:11 AM (10 years, 1 month ago) |
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i'm gonna purposely inoculate a plate or two with some bacteria to see if this is really a viable solution for bacterial prevention. jars are cooling now!
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19254445 - 12/10/13 09:16 AM (10 years, 1 month ago) |
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 this is a pretty nasty one, gonna take a little of the bacteria and a little chunk of the mycelium
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19254869 - 12/10/13 11:14 AM (10 years, 1 month ago) |
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just a word of caution pastye don't let your agar cool for more than two hours before adding the tetracycline, i was so worried about thermal degradation of the antibiotic i let the jars almost solidify and mixing the tetracycline in almost became impossible so i have them warming up slightly on the stove to see if i can get it to mix better
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19254891 - 12/10/13 11:20 AM (10 years, 1 month ago) |
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Noted
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lessismore
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19254936 - 12/10/13 11:34 AM (10 years, 1 month ago) |
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my LC died after 3-4 months in the refridgerator, in the in the vegetable room
sucks, and not first time it has happened
wonder how to avoid that... it would probably have survived at roomtemp a syringe I took from it had survived that long in roomtemp
should LCs not be placed in the refridgerator?
how long can LC be stored at roomtemp / fridge?
(btw I used honey LC, but it had plenty of nutrients left I think..)
and if my LC doesnt die it starts to get bacteria in somehow magically after a few months... bacteria love honey water it seems
might try out this tek :-) , potatoes and agar and corn syrup is easy to find
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: lessismore]
#19254950 - 12/10/13 11:37 AM (10 years, 1 month ago) |
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For long term storage of a culture nothing beats a slant
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: lessismore]
#19255010 - 12/10/13 11:49 AM (10 years, 1 month ago) |
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Quote:
mio said: my LC died after 3-4 months in the refridgerator, in the in the vegetable room
sucks, and not first time it has happened
wonder how to avoid that... it would probably have survived at roomtemp a syringe I took from it had survived that long in roomtemp
should LCs not be placed in the refridgerator?
how long can LC be stored at roomtemp / fridge?
(btw I used honey LC, but it had plenty of nutrients left I think..)
and if my LC doesnt die it starts to get bacteria in somehow magically after a few months... bacteria love honey water it seems
might try out this tek :-) , potatoes and agar and corn syrup is easy to find
avoid LC dying by not doing them like pasty said long term storage is best done in a culture slant.
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19255128 - 12/10/13 12:16 PM (10 years, 1 month ago) |
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poured 8 plates 4 a little heavier only slightly on the tetracycline gonna do two plates with the known contaminated bacteria and mycelium, two with nothing on them just plates to see if anything decides to pop up without interference two with just bacteria and two with good mycelium. i know this is way off topic from my original thread but i just wanted to share.
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19255173 - 12/10/13 12:25 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: just a word of caution pastye don't let your agar cool for more than two hours before adding the tetracycline, i was so worried about thermal degradation of the antibiotic i let the jars almost solidify and mixing the tetracycline in almost became impossible so i have them warming up slightly on the stove to see if i can get it to mix better 
wug, ive done this numerous times and it takes forever to melt back down

let us know how the swab of bacteria goes on the antibiotic mix
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19255185 - 12/10/13 12:27 PM (10 years, 1 month ago) |
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another fun one to do is to take some metabolites from a grain jar(you can just stress them with heat over a few days)suck them up in a syringe and drop them on top of the bacteria on agar
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19255904 - 12/10/13 03:20 PM (10 years, 1 month ago) |
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since i put this out there and i'm getting new prints sometime this week as well as my new dishes and cloning tools i'm thinking about starting a grow a long "spore print to spore print" a full start to finish to start grow! anyone else down?
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19255941 - 12/10/13 03:29 PM (10 years, 1 month ago) |
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i'm down
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19255944 - 12/10/13 03:30 PM (10 years, 1 month ago) |
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Cool it'll be my first time working with prints! I'll start working on getting the thread setup tonight. I'm gonna try to leave it open for different techniques unless it's just total BS
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19255969 - 12/10/13 03:38 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: Cool it'll be my first time working with prints! I'll start working on getting the thread setup tonight. I'm gonna try to leave it open for different techniques unless it's just total BS
just do itttt
its fun
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19256007 - 12/10/13 03:45 PM (10 years, 1 month ago) |
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I haven't had any luck with prints. I've only tried germinating P.Galindoi. I have tried 4 different ways without any luck.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19256019 - 12/10/13 03:48 PM (10 years, 1 month ago) |
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i'm getting some galindoi as well but i think i'm gonna try focusing on cubes, i hope i have some luck it would suck to start a grow along and get stuck not being able to get the spore prints to germinate
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19256373 - 12/10/13 05:03 PM (10 years, 1 month ago) |
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I have always had fantastic success with prints to agar. The I got a galindoi print from a sponsor and it would not germinate, and I tried many things. Well I emailed him saying the print was not viable and he replied back saying that they had a bad batch of prints that would not germinate but would send me a replacement. Then it was suggested that in the meantime i make a spore syringe to hydrate my spores and for me to use that to inoculate my agar. Shit i almost fell over cause i was real careful to word my email so that it did not explicitly sound like i was growing. Then i get a response suggesting ways for me to grow them Great vendor.
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19256487 - 12/10/13 05:25 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said: I have always had fantastic success with prints to agar. The I got a galindoi print from a sponsor and it would not germinate, and I tried many things. Well I emailed him saying the print was not viable and he replied back saying that they had a bad batch of prints that would not germinate but would send me a replacement. Then it was suggested that in the meantime i make a spore syringe to hydrate my spores and for me to use that to inoculate my agar. Shit i almost fell over cause i was real careful to word my email so that it did not explicitly sound like i was growing. Then i get a response suggesting ways for me to grow them Great vendor.
lolll. my first pe prints didnt germinate but the ones i have now did and are cased and fruiting. i would hydrate your spores for best luck
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Icesyn
Trust My Words


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19256535 - 12/10/13 05:32 PM (10 years, 1 month ago) |
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You made agar look like a noob-friendly tek...
You totally should rename this "The Potato Jello Tek"
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Pastywhyte
Say hello to my little friend



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Re: Just say NO to LC and YES to PDYA [Re: Icesyn]
#19256579 - 12/10/13 05:40 PM (10 years, 1 month ago) |
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That's what we had been arguing throughout this whole thread, agar is not hard. Once you dip your toe into the agar pool its kinda hard to stop. Guaranteed that any "noob" who finds a decent clone or isolate is gonna be making slants a week later.
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19256581 - 12/10/13 05:41 PM (10 years, 1 month ago) |
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agar..my favorite part of the job
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19256590 - 12/10/13 05:42 PM (10 years, 1 month ago) |
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Quote:
cronicr said: agar..my favorite part of the job
QFT
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Icesyn]
#19256731 - 12/10/13 06:10 PM (10 years, 1 month ago) |
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Quote:
Icesyn said: You made agar look like a noob-friendly tek...
You totally should rename this "The Potato Jello Tek"

i seriously was rofl had to show my wife that one she busted up laughing!!!
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19256843 - 12/10/13 06:29 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Quote:
Icesyn said: You made agar look like a noob-friendly tek...
You totally should rename this "The Potato Jello Tek"

i seriously was rofl had to show my wife that one she busted up laughing!!!
someone used yams, so in theory i bet it would be edible and might not taste bad
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Icesyn
Trust My Words


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19257707 - 12/10/13 09:18 PM (10 years, 1 month ago) |
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Quote:
twistedty said:
Quote:
blueconfusion said:
Quote:
Icesyn said: You made agar look like a noob-friendly tek...
You totally should rename this "The Potato Jello Tek"

i seriously was rofl had to show my wife that one she busted up laughing!!!
someone used yams, so in theory i bet it would be edible and might not taste bad
Sweet Potato Jello Tek?
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Icesyn]
#19259428 - 12/11/13 10:14 AM (10 years, 1 month ago) |
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I plan on finishing everything on Friday should be getting all my plates tomorrow but I won't have time basically just reheating agar and pouring. Which will also be posted on the grow along.
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mushrume man
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19266563 - 12/12/13 04:05 PM (10 years, 1 month ago) |
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Here is an interesting link that I found the other day, it's basically the same as the PYDA recipe in the OP.
Quote:
SpitballJedi said: I haven't had any luck with prints. I've only tried germinating P.Galindoi. I have tried 4 different ways without any luck.
WTF I must be lucky. I have had every spore germinate I've put to agar. Some took weeks but eventually grew just fine. Shiitake was especially slow
Quote:
blueconfusion said:...24 cakes and a plate or two of agar from one syringe... and i don't need syrup water to do that i just use some distilled water. I shoot a few cakes up and then suck up some distilled water shake and keep going.
Are you talking about stretching out a spore syringe by topping up w distilled water between injections?
Just have to say that agar is the best thing for mycology since sliced bread. I love how it keeps things going in the down time and it is real easy to prep. The best part is that its a great reflection of your sterile procedures with quick results.
--------------------
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: mushrume man]
#19269634 - 12/13/13 07:26 AM (10 years, 1 month ago) |
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 gee is this a contamination or is it mycelium guess i'll have to knock up some test jars to find out , this one i made from a leftover stray grain in my glovebox one transfer later looking clean and sectoring beautifully, tell me how you would/could see this in an LC? by the time this is done i'll be able to knock up several jars and G2G those without any test jars, or worry of is this contamination or mycelium. i can easily make slants from this for long term storage if i so choose as well, which is what i'll probably do. i'll finish up the pics today as soon as i get some time.
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270273 - 12/13/13 11:14 AM (10 years, 1 month ago) |
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ive said it before and ill say it again LC is an outdated tek. great work
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19270392 - 12/13/13 11:43 AM (10 years, 1 month ago) |
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 25 dishes poured!!! took a little over 500mL, but i poured a little thick.
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270399 - 12/13/13 11:45 AM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
 25 dishes poured!!! took a little over 500mL, but i poured a little thick.
just like i like my women.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19270415 - 12/13/13 11:49 AM (10 years, 1 month ago) |
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booty baby got the thickness can i get a witness shake it like she's fearless can i get a witness HELL YEAH!!! sorry puscifer instantly popped in my head 
Edited by blueconfusion (12/13/13 01:08 PM)
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sharpshroomer98
Student of the Universe.



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270685 - 12/13/13 01:06 PM (10 years, 1 month ago) |
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So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
-------------------- No, I wasn’t born in 98’
 
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blueconfusion
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Quote:
sharpshroomer98 said: So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
basically
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spacechildo
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270718 - 12/13/13 01:13 PM (10 years, 1 month ago) |
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Nice going blue! I like your new threads, cool to follow a start to finish grow with all the Q's and comments that goes along
+5
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sharpshroomer98
Student of the Universe.



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270725 - 12/13/13 01:16 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Quote:
sharpshroomer98 said: So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
basically
Well I'm glad this works because I was going to have to do the same thing. Whiskey bottles be too big for my modest PC lol.
Great write up man, and can't wait for more updates
-------------------- No, I wasn’t born in 98’
 
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: spacechildo]
#19270751 - 12/13/13 01:26 PM (10 years, 1 month ago) |
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Quote:
spacechildo said: Nice going blue! I like your new threads, cool to follow a start to finish grow with all the Q's and comments that goes along
+5 
thanks i've written a lot of SOP's
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lessismore
Registered: 02/10/13
Posts: 6,268
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Quote:
sharpshroomer98 said:
Quote:
blueconfusion said:
Quote:
sharpshroomer98 said: So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
basically
Well I'm glad this works because I was going to have to do the same thing. Whiskey bottles be too big for my modest PC lol.
Soya sauce glass bottles work great, they even come with that "wine" fitting making it easier to pour, but usually only around 300mL (500mL is more ideal IMO)
also perhaps small shaker bottles, they are usually microwave safe too for re-heating
and they are usually up to 500-700ml, should work for PCing too with the lid losened a bit I think
agar is easy to sterilize like any liquid
haven't experimented with microwave though... but it might work and make the job easier it will only be a short testing I want to try one day.. I still only trust my PC
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spacechildo
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270870 - 12/13/13 01:52 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Quote:
spacechildo said: Nice going blue! I like your new threads, cool to follow a start to finish grow with all the Q's and comments that goes along
+5 
thanks i've written a lot of SOP's 
Isn't that what it's all about? Reinventing the teks as everyone use different tools and whatnots. Maybe even rephrase some words to give some of us a "aha" moment. Much more interesting than the everyday mono or pf-tek trouble to me.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: spacechildo]
#19271253 - 12/13/13 03:21 PM (10 years, 1 month ago) |
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oh the antibiotic agar using tetracycline which you can find at lfs the mycelium is showing some recovery and the bacteria is just sitting there... i did 1 with just bacteria 1 with bacteria and myc. 1 with just myc. and 1 with nothing opened in open air just the hell of it...
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Camtaro420
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19271438 - 12/13/13 04:06 PM (10 years, 1 month ago) |
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Does the yeast have to be nutritional yeast? OR can it be a different type like bread yeast etc.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19271484 - 12/13/13 04:19 PM (10 years, 1 month ago) |
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no nutritional yeast is actually "deactivated yeast" so it's dead while bread yeast are alive and active
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Camtaro420
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19271488 - 12/13/13 04:20 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: no nutritional yeast is actually "deactivated yeast" so it's dead while bread yeast are alive and active
Gotcha. Thanks for the quick reply.
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SpitballJedi
Ancient Astronaut



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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19271501 - 12/13/13 04:23 PM (10 years, 1 month ago) |
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nutritional yeast is great on popcorn
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Camtaro420
Birdman



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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19271523 - 12/13/13 04:29 PM (10 years, 1 month ago) |
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So it's 500 ML of water. 150G of potatoes to boil in the water. add 9 grams of dextrose and 1 gram of yeast to that cooked and then filtered potato water, then 9 grams of agar.
So to that I ask
Hoe much potato flakes should I use instead of 150g of potatoes for 500 ML of water?
How many grams of Karo should I sub for the 9 grams of dextrose?
My yeast question was answered thanks.
And is agar agar ok for agar? And same 9 grams?
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Camtaro420
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19271527 - 12/13/13 04:29 PM (10 years, 1 month ago) |
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Quote:
SpitballJedi said: nutritional yeast is great on popcorn
Hell yeah it is.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19271560 - 12/13/13 04:37 PM (10 years, 1 month ago) |
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ok i think if you go here you'll get your recipe questions and yes 500mL water 150g potatoes boil strain add dextrose and yeast simmer take off heat and add agar or agar agar (same thing) slowly stirring with a whisk or fork it will clump!
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Camtaro420
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19271574 - 12/13/13 04:40 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: ok i think if you go here you'll get your recipe questions and yes 500mL water 150g potatoes boil strain add dextrose and yeast simmer take off heat and add agar or agar agar (same thing) slowly stirring with a whisk or fork it will clump!
That's perfect thanks!
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krypto2000
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19271576 - 12/13/13 04:40 PM (10 years, 1 month ago) |
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I just made some pdya the other day though with sweet potatoes. If I get a nice clean culture I'm going to use it to make a LC .
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mushrume man
Sadis Factory



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Re: Just say NO to LC and YES to PDYA [Re: krypto2000]
#19271655 - 12/13/13 05:01 PM (10 years, 1 month ago) |
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Just say NO!
--------------------
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krypto2000
Unknown


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Re: Just say NO to LC and YES to PDYA [Re: mushrume man]
#19271680 - 12/13/13 05:08 PM (10 years, 1 month ago) |
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What's the problem with LC if you know it's clean?
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: krypto2000]
#19271687 - 12/13/13 05:09 PM (10 years, 1 month ago) |
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Quote:
krypto2000 said: What's the problem with LC if you know it's clean?
nuthing
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19271702 - 12/13/13 05:12 PM (10 years, 1 month ago) |
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why...wait...did...no...nevermind...
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SpitballJedi
Ancient Astronaut



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Re: Just say NO to LC and YES to PDYA [Re: krypto2000]
#19271706 - 12/13/13 05:13 PM (10 years, 1 month ago) |
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Nothing, if it's clean. But you never really know if it's clean. It can be clean for a while, then contams show up. It's just unreliable.
Personally, I just have no use for LC. I find agar and G2G to be more reliable. But, to each his own.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
Edited by SpitballJedi (12/13/13 05:14 PM)
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Camtaro420
Birdman



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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#19272422 - 12/13/13 08:53 PM (10 years, 1 month ago) |
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I have a feeling this isn't the right stuff but it was all I could find.


Is this usable?
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PussyFart
Retired Cultivation Extrodinaire



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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19272439 - 12/13/13 08:58 PM (10 years, 1 month ago) |
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Try it and let us know.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Camtaro420
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Re: Just say NO to LC and YES to PDYA [Re: PussyFart]
#19272445 - 12/13/13 08:59 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said: Try it and let us know.
Are B vitamins known to affect myc growth at all?
All it is is nutritional yeast with various B vitamins.
EDit: and folic acid?
Edited by Camtaro420 (12/13/13 09:00 PM)
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19272716 - 12/13/13 10:16 PM (10 years, 1 month ago) |
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Same stuff I have
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Camtaro420
Birdman



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19272999 - 12/13/13 11:46 PM (10 years, 1 month ago) |
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Sweeet.
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newera
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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19273029 - 12/13/13 11:56 PM (10 years, 1 month ago) |
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haha i'm doing agar and lc!
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Camtaro420
Birdman



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Re: Just say NO to LC and YES to PDYA [Re: newera]
#19273043 - 12/14/13 12:00 AM (10 years, 1 month ago) |
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Quote:
newera said: haha i'm doing agar and lc!
I too am going to work with an LC after my agar iso's are done and I got a nice healthy isolated growth. Only because LC seems to be easier for BRF cakes.
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krypto2000
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Re: Just say NO to LC and YES to PDYA [Re: Camtaro420]
#19273676 - 12/14/13 07:40 AM (10 years, 1 month ago) |
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Yeast makes b vitamins so they might not even be added and just listed for marketing reasons to make it look healthier.
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loki44
non serviam



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Re: Just say NO to LC and YES to PDYA [Re: krypto2000]
#19275302 - 12/14/13 03:57 PM (10 years, 1 month ago) |
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agar is not needed guys, GLC is easier faster and high chance of no contamination
you guys miring my sexy grains inoculated with it? 

now...on the same subject..I just bit the bullet and picked up 6 glass petri dishes on ebay for $14.80 so I will be paying a visit to asian market and local brewery store soon LOL
I want to clone some pins , see what comes out of it , wish me luck fellas
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275310 - 12/14/13 04:00 PM (10 years, 1 month ago) |
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thats alot of doh for 6 dishes, and g2g is quicker
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275314 - 12/14/13 04:00 PM (10 years, 1 month ago) |
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Quote:
loki44 said: agar is not needed guys, GLC is easier faster and high chance of no contamination

if you want to stick to ms the whole time and not evolve your myco skills and success and yields etc etc etc etc etc etc etc etc
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19275316 - 12/14/13 04:01 PM (10 years, 1 month ago) |
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Quote:
cronicr said: thats alot of doh for 6 dishes, and g2g is quicker
and glass dishes are a PITA to work with also
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loki44
non serviam



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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19275336 - 12/14/13 04:03 PM (10 years, 1 month ago) |
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Quote:
cronicr said: thats alot of doh for 6 dishes, and g2g is quicker
I can use them forever, less waste for the environment and cost effective on the long term, so no
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275347 - 12/14/13 04:05 PM (10 years, 1 month ago) |
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to each his own
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PussyFart
Retired Cultivation Extrodinaire



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275387 - 12/14/13 04:11 PM (10 years, 1 month ago) |
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Quote:
loki44 said: I can use them forever
Only if they never break.....
Quote:
loki44 said: less waste for the environment
Plastics get recycled.......
Quote:
loki44 said: cost effective on the long term, so no
Only if they never break.....
Just sayin.....plastic petri dishes are more durable.
That and I can get 500 petri dishes for the same price you can get 24 glass dishes.....
I can do a lot more projects in a shorter amount of time.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275469 - 12/14/13 04:30 PM (10 years, 1 month ago) |
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Quote:
loki44 said: you guys miring my sexy grains inoculated with it? 

Not until I see fruits. You could have all kinds of nasty shit in those jars just waiting for you to spawn before they pop up.
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loki44
non serviam



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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19275499 - 12/14/13 04:36 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said:
Not until I see fruits. You could have all kinds of nasty shit in those jars just waiting for you to spawn before they pop up.
impossible, I inoculated 13 brf jars with the same GLC and they are 100% colonized clean, so no nasties
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275507 - 12/14/13 04:37 PM (10 years, 1 month ago) |
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 try start your own thread though, y stop in here and tell people not to use agar...kinda a dick move
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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twistedty
Forcefully Retired



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275512 - 12/14/13 04:39 PM (10 years, 1 month ago) |
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Quote:
loki44 said:
Quote:
Pastywhyte said:
Not until I see fruits. You could have all kinds of nasty shit in those jars just waiting for you to spawn before they pop up.
impossible, I inoculated 13 brf jars with the same GLC and they are 100% colonized clean, so no nasties
not impossible by any means
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: PussyFart]
#19275531 - 12/14/13 04:45 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
loki44 said: I can use them forever
Only if they never break.....
Quote:
loki44 said: less waste for the environment
Plastics get recycled.......
Quote:
loki44 said: cost effective on the long term, so no
Only if they never break.....
Just sayin.....plastic petri dishes are more durable.
That and I can get 500 petri dishes for the same price you can get 24 glass dishes.....
I can do a lot more projects in a shorter amount of time.
how are they more durable when is one and done ,plastic is a huge hazard these days with all the tons going into the oceans etc, glass on the contrary is 100% recyclable into new glass, no downgrading
buying 500 petri dishes to clean up or clone a couple cultures every now and then is overkill!
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19275550 - 12/14/13 04:51 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
 try start your own thread though, y stop in here and tell people not to use agar...kinda a dick move
Im not spamming this thread with a million posts dude,actually my post with a pic of my GLC and grain jars was with sort of tongue in cheek, because I decided to use agar theresnoneedtobeupset.jpg
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275555 - 12/14/13 04:51 PM (10 years, 1 month ago) |
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its always better to buy in bulk
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Pestile

Registered: 05/02/13
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275563 - 12/14/13 04:53 PM (10 years, 1 month ago) |
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Both have pros and cons, but if you are planning on doing a lot of transfers then plastic>glass. But glass still has its charms.
--------------------
   The Corbett Report Open Source Intelligence News
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275575 - 12/14/13 04:56 PM (10 years, 1 month ago) |
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Quote:
loki44 said: agar is not needed guys,
Quote:
loki44 said: actually my post with a pic of my GLC and grain jars was with sort of tongue in cheek, because I decided to use agar theresnoneedtobeupset.jpg
i'm just bugging bro, no harm intended and discussion is the backbone to knowledge
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Stromrider
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Re: Just say NO to LC and YES to PDYA [Re: Pestile]
#19275582 - 12/14/13 04:58 PM (10 years, 1 month ago) |
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Loki you'll figure it out in time. There's a reason why all the pros use agar
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19275583 - 12/14/13 04:58 PM (10 years, 1 month ago) |
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Quote:
twistedty said: its always better to buy in bulk 
if you are doing 30 monotubs sure, maybe 1000 petris would be better if you can get a better deal
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twistedty
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19275586 - 12/14/13 04:59 PM (10 years, 1 month ago) |
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Quote:
Stromrider said: Loki you'll figure it out in time. There's a reason why all the pros use agar 
and alot of dishes
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Stromrider
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19275591 - 12/14/13 04:59 PM (10 years, 1 month ago) |
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loki44
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19275709 - 12/14/13 05:24 PM (10 years, 1 month ago) |
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Quote:
twistedty said:
Quote:
Stromrider said: Loki you'll figure it out in time. There's a reason why all the pros use agar 
and alot of dishes 
no doubt in my mind why the pros use agar , no argument there
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Pastywhyte
Say hello to my little friend



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Re: Just say NO to LC and YES to PDYA [Re: loki44]
#19275722 - 12/14/13 05:28 PM (10 years, 1 month ago) |
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Shiiiit, I ain't no pro, but I love agar anyways. So many reasons, clean inoculate, always something to do, its fun and easy. Having a few cultures then fruiting them all to see which does best is like betting on the horses. Finding that excellent culture that makes it into the slant collection is always a plus. Agar is not just for pros, noobs like me can have just as much fun with it
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276033 - 12/14/13 06:47 PM (10 years, 1 month ago) |
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Damn missed out on good discussion no pro here but i love agar! I can select for the best growth and have so much more fun than g2g which gets boring...Sorry true hobbist here I plan on really stepping up my game from here agar opens so many doors for edibles and exotics!!!
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276104 - 12/14/13 07:05 PM (10 years, 1 month ago) |
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i've reused a couple of my plastic dishes, you can't boil or pc them but i have had zero problems cleaning them. i used old dishes with my antibiotic agar which seems to be holding the bacteria at bay and letting the myc. grow even the dishes i purposely put bacteria in...plus the nice thing about plastic dishes is if one gets overrun by a contaminate that ran a muck you don't feel as bad about just tossing it out...
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Stromrider
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276111 - 12/14/13 07:06 PM (10 years, 1 month ago) |
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Yeah you can't lose sight of the fact that working intimately with your culture on agar is very rewarding and enjoyable. It also teaches you a lot about the mycelium itself and how it grows
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Pastywhyte
Say hello to my little friend



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276118 - 12/14/13 07:07 PM (10 years, 1 month ago) |
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That's one of the attractions of pp5 agar, you can reuse that shit till the cows come home. Back into the PC it goes
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276131 - 12/14/13 07:10 PM (10 years, 1 month ago) |
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yeah i think if i need to buy anymore dishes i'm just gonna go with the pp5 tupperware, so much easier to find and you're right i can just throw it in the pc! and use it forever!!! and i can do no pour if i so choose
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276153 - 12/14/13 07:14 PM (10 years, 1 month ago) |
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so loki you gonna quit crashing my thread? maybe start your own something like "how to make every noob a nervous wreck with GLC's (the worst advice a noob can give a noob)" idk kinda wordy but it's a working title
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Aero
Orea


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276179 - 12/14/13 07:19 PM (10 years, 1 month ago) |
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agar is just so easy mea for president!
-------------------- SPREAD THE SPORES
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Stromrider
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Re: Just say NO to LC and YES to PDYA [Re: Aero]
#19276192 - 12/14/13 07:21 PM (10 years, 1 month ago) |
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Don't be too hard on him blue. It's a rainy Saturday night with nothing to do and he did give us something to talk about
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Aero]
#19276197 - 12/14/13 07:21 PM (10 years, 1 month ago) |
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mea is really easy too!!! you ever try putting yeast in that i've read that works really well.
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276223 - 12/14/13 07:27 PM (10 years, 1 month ago) |
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i just did some lc's but shhhh........
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276232 - 12/14/13 07:28 PM (10 years, 1 month ago) |
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Quote:
Stromrider said: Don't be too hard on him blue. It's a rainy Saturday night with nothing to do and he did give us something to talk about
i'm just razzing him not raining so much as a blizzard here, and i always appreciate a good discussion. i know i was all about glc's and lc's one time myself...thought i had found the best thing since sliced bread! well until everything either went bad or contaminated and i started looking into making agar which was incredibly intimidating at first, but i will never go back...i haven't done an lc in over a year once i started doing g2g i knew i could do sterile work and gave me the courage to start doing agar!
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Stromrider
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19276235 - 12/14/13 07:29 PM (10 years, 1 month ago) |
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I did some galindoi LC but I used an agar wedge to inoculate it
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276238 - 12/14/13 07:30 PM (10 years, 1 month ago) |
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yeah i always use a wedge, lc's are great for my species list and almost an asset
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Stromrider
This must be the place



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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276241 - 12/14/13 07:31 PM (10 years, 1 month ago) |
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Man I wish we could get some snow down south
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276247 - 12/14/13 07:32 PM (10 years, 1 month ago) |
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got plenty here something like 6-12"!
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Aero
Orea


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion] 1
#19276259 - 12/14/13 07:34 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: mea is really easy too!!! you ever try putting yeast in that i've read that works really well.
i dont have yeast atm, but gonna give it a whirl
-------------------- SPREAD THE SPORES
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Stromrider
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276264 - 12/14/13 07:35 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said: got plenty here something like 6-12"!
You suck
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Pastywhyte
Say hello to my little friend



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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276289 - 12/14/13 07:42 PM (10 years, 1 month ago) |
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I'm not even going to start on about the 24"+ that I am cursing right fucking now. Plus its cold as fuck, got down to -20f last week. Fucking bullshit. Barely can keep my grow area above 69F, everything is going slow as fuck.
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sharpshroomer98
Student of the Universe.



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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276302 - 12/14/13 07:44 PM (10 years, 1 month ago) |
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Damn I thought here with the greatest snow on earth we had it bad lol. We've been under 10 the last week or so. Damn cold.
-------------------- No, I wasn’t born in 98’
 
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Stromrider
This must be the place



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Man screw all yyou guys! I never get snow!
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cronicr



Registered: 08/07/11
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276329 - 12/14/13 07:48 PM (10 years, 1 month ago) |
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i'm up in bc and rarely get it, and when it does come it goes as fast as it showed up
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19276342 - 12/14/13 07:51 PM (10 years, 1 month ago) |
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It could be worse. A few years back it got down to -53.5F. I was tempted to build an incubator at that point 
Quote:
cronicr said: i'm up in bc and rarely get it, and when it does come it goes as fast as it showed up
Screw you Van island pussies
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Stromrider
This must be the place



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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276349 - 12/14/13 07:53 PM (10 years, 1 month ago) |
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Uh oh its about to get ugly in here. Well as ugly as it can get by Canadians
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cronicr



Registered: 08/07/11
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276355 - 12/14/13 07:54 PM (10 years, 1 month ago) |
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Quote:
Screw you Van island pussies 
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19276361 - 12/14/13 07:55 PM (10 years, 1 month ago) |
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and i'm still waiting on that contam timelapse....just give your vag a wipe n swipe it to agar for me baby!
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276371 - 12/14/13 07:57 PM (10 years, 1 month ago) |
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Quote:
Stromrider said: Uh oh its about to get ugly in here. Well as ugly as it can get by Canadians 
Shit, when you live in Oilberta it can get damn ugly. I got lost driving through L.A. once and found out I was in the Ghetto. I'm like "damn" this is nicer than my hood Take a drive through the reservations round here and ya see bullet riddled pig cruisers on fire on the corners
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cronicr



Registered: 08/07/11
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276386 - 12/14/13 08:01 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said: Take a drive through the reservations round here and ya see bullet riddled pig cruisers on fire on the corners 
hell yeah and AMEN!
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ranonar
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Re: Just say NO to LC and YES to PDYA [Re: TiN 42]
#20138219 - 06/16/14 06:15 AM (9 years, 7 months ago) |
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A few small adaptations to this recipe:
I would rather use potato powder (potato starch) than fresh potatoes. I rather use marmite (= yeast&vegetable extract) than just yeast I do not pour the liquid in petris but transfer it from the bottle to the petris with a large (20ml) syringe.
4.0 grams potato starch 20.0 grams dextrose 15.0 grams agar 2 grams of Marmite
I have dissolved one small jar of Marmite in 250ml water. Filled a bottle with it and sterilized that one. Now add 4-5ml of this in 1 liter of PDA or other agar medium.
I use a syringe for liquid agar transfer because pouring agar often causes spills, and then those become a vector for contamination.
About the (G)LC versus agar discussion: I think it is a bit more complicated since it mattters if you are inoculating a master or an end substrate. I am on the side of RR and others in that a 2 dimensional (agar) surface -which is itself inoculated by a pinch of spores, or a wedge of agar or a kernel of spawn- is the most secure way to avoid contaminations. But it is perfectly fine to use an agar plate to make a liquid inoculant for immediate use.
I hesitate to recommend a liquid inoculant to be used on any substrate which needs to be shaken. After the shake there is no way to find out the origin of a contamination. Liquid inoculants are fine for a BRF/verm (PF) substrate but injecting it in bags or jars of whole grain or rye grass substrates adds some risk. Which is managable as long as those bags or jars are not to be used as grain masters (to inoculate more grain jars).
IMHO future Grain Masters need to be inoculated by agar wedge. I think that is the central dogma.
Liquid inoculants are fine for end substrates such as PF jars and grains which are to be cased and fruited. Be it that liquid inoculants are risky for substrates which are to be shaken.
To make it even more complicated, in my observation LCs can have a better shelf life than spore prints. At least up to 10 years at room temp. But these long term storage LCs are not easy to prepare. They need to be stored in a special way. And need to be tested a month or so after they are made and then once a year. Invisible contamination is a huge risk with any stored LC.
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MonkeyJesusFresco
am i suspended in agar?



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Re: Just say NO to LC and YES to PDYA [Re: ranonar]
#20138370 - 06/16/14 07:40 AM (9 years, 7 months ago) |
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oh...so the "just say no" campaign...that a thing huh?
-------------------- LAGM v 2.024 - endo cabendo
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ranonar
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I do not know if it is a real campaign. But it is a nice banner I wonder in which thread it first appeared and why exactly. But I am following the (sometimes heated) discussions about LC closely. They are interesting!
But the discussions are often not detailed enough. There is a difference between LC and LI (liquid inoculant). An LI can be drawn from an LC or freshly made from a colonized solid substrate. The latter is often a good idea.
All over the internet LC's are promoted as a good tek to stretch a syringe. And -IMHO- that is often not a good idea because of the danger for hidden contaminants. for that reason I think it is a good idea to get a warning out. The 'campaign' is a good idea. If you want a real safe way to stretch a syringe, then inoculate a BRF/verm jar, use a small block of it to put on an agar plate, wait until that is colonized and make a LI from that.
But subculturing agar plates over and over again is also a fast route to degeneration. You need something for long term storage, and spores are not always an option. Blocks of colonized agar in sterile water is the best known alternative but it is not super convenient (a SAB or other sterile environment is required to handle those). So if you really know what you are doing and know how to handle the risks then LCs can indeed be a viable option to save a culture for a decade or more at roomtemperature.
For me - I try to avoid LCs. But I use LIs quite often. And sometimes I depend on LCs for long time storage. When I look at it that way the 'campaign' goes too far. But perhaps I am taking it all to serious.
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SpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: ranonar]
#20139847 - 06/16/14 02:06 PM (9 years, 7 months ago) |
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Quote:
ranonar said: But the discussions are often not detailed enough. There is a difference between LC and LI (liquid inoculant). An LI can be drawn from an LC or freshly made from a colonized solid substrate. The latter is often a good idea.
So wait, LC can't be drawn from an LC or freshly made from a colonized solid substrate?
Why would one be better than the other?
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MonkeyJesusFresco
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#20140573 - 06/16/14 04:50 PM (9 years, 7 months ago) |
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Get this:
to prepare a LC, you whip up some water and nutrients add spores/mycelium and...TA-DA! LC.
L.I. is a new term for me, but I imagine it's like a "grain"-LC. where you take your clean, fully colonized grain jar, add sterile water, shake withdraw the liquid (that now has little bits of mycelium floating in it) and...TA-DA! a liquid inoculation.
The benefit there being, that there's not an excess of nutrients for contamination to run rampant on..
just my 2cents though
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PussyFart
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Quote:
MonkeyJesusFresco said: L.I. is a new term for me, but I imagine it's like a "grain"-LC. where you take your clean, fully colonized grain jar, add sterile water, shake withdraw the liquid (that now has little bits of mycelium floating in it) and...TA-DA! a liquid inoculation.
The benefit there being, that there's not an excess of nutrients for contamination to run rampant on..
If there is enough nutrients for the LC to grow afterwards, there is enough for contams.
--------------------
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MonkeyJesusFresco
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Re: Just say NO to LC and YES to PDYA [Re: PussyFart]
#20140626 - 06/16/14 05:00 PM (9 years, 7 months ago) |
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I've actually dreamed up a few contraptions/techniques for filtering out and "washing" the mycelial growth of a LC, washing away excess nutrients and whatever the word for "fungus-poop" is; leaving a sterile mycellium suspension...it's called the "Fuck that!"-tek
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SpitballJedi
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#20140793 - 06/16/14 05:49 PM (9 years, 7 months ago) |
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I've evolved my tek quite a bit since I wrote this.
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blindingleaf
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20141116 - 06/16/14 06:58 PM (9 years, 7 months ago) |
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Quote:
blueconfusion said:
I've evolved my tek quite a bit since I wrote this.
whats up blue!!
what kinda stuff u doing now compared to the tek?
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ranonar
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Quote:
to prepare a LC, you whip up some water and nutrients add spores/mycelium and...TA-DA! LC.
True. And a good pointer to the most risky technique! Spores should never be germinated in a liquid. Mycelium should almost never not be grown in a liquid. Ideally the liquid phase should only exist for the purpose of inoculation. It should be made from colonized agar or BRF/verm or whole grain spawn. That is the liquid inoculant (LI)
You may inoculate with a liquid but do the growing on something solid if you want to keep te contam risk down.
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: ranonar]
#20144280 - 06/17/14 10:38 AM (9 years, 7 months ago) |
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Quote:
ranonar said: You may inoculate with a liquid but do the growing on something solid if you want to keep te contam risk down.

I don't think anyone has been able to grow on liquid yet. RR had a contest a few years back for a microscope for anyone who could grow a 10gram wet fruit on liquid. I do not believe he ever had to pay out
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MonkeyJesusFresco
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#20144408 - 06/17/14 11:14 AM (9 years, 7 months ago) |
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he ment growing mycelium not fruiting
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Pastywhyte
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Still does not make sense 
I guess I find most of the terminology fairly sufficient. Liquid culture is liquid culture. Multispore solution is multispore solution. Easy. I personally like LC for a few things. I don't like the idea that its easy or noob friendly. To do it right takes care and sterile tek that you will not learn firing spores into sugar water. But I still have some fun with it
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nucleophile
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#20144547 - 06/17/14 11:47 AM (9 years, 7 months ago) |
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I fruited a mushroom in an LC once. I'd call it inefficient at best, hehe.
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Dos Ounce
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Re: Just say NO to LC and YES to PDYA [Re: PussyFart]
#20144613 - 06/17/14 12:04 PM (9 years, 7 months ago) |
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Quote:
PussyFart said:
Quote:
MonkeyJesusFresco said: L.I. is a new term for me, but I imagine it's like a "grain"-LC. where you take your clean, fully colonized grain jar, add sterile water, shake withdraw the liquid (that now has little bits of mycelium floating in it) and...TA-DA! a liquid inoculation.
The benefit there being, that there's not an excess of nutrients for contamination to run rampant on..
If there is enough nutrients for the LC to grow afterwards, there is enough for contams.
I don't think there is :/ maybe I misinterpreted but I think it is basically just to hold the mycelia solution until you use it. Don't think the mycelia actually "grows" in it.
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Pastywhyte
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Re: Just say NO to LC and YES to PDYA [Re: Dos Ounce]
#20144630 - 06/17/14 12:08 PM (9 years, 7 months ago) |
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Trich is predatory and will feed on the mushroom myc. In that case there would always be nutes for it.
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Dos Ounce
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#20144636 - 06/17/14 12:08 PM (9 years, 7 months ago) |
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ahh, that makes sense
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Djchrist
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Re: Just say NO to LC and YES to PDYA [Re: Dos Ounce]
#20148529 - 06/18/14 05:59 AM (9 years, 7 months ago) |
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AIRDOG



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20537749 - 09/08/14 01:06 PM (9 years, 4 months ago) |
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can I use bakers yeast? its the only one I have
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: AIRDOG]
#20537821 - 09/08/14 01:24 PM (9 years, 4 months ago) |
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Don't use bakers yeast it's not the same thing just forget the yeast it's not necessary
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cronicr



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20537946 - 09/08/14 01:47 PM (9 years, 4 months ago) |
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#20539246 - 09/08/14 05:59 PM (9 years, 4 months ago) |
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Read a bunch of old posts on here I wonder if loki ever did his glc
Edited by blueconfusion (09/09/14 02:53 AM)
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Stromrider
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20539992 - 09/08/14 07:54 PM (9 years, 4 months ago) |
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Who cares
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#20541702 - 09/09/14 05:57 AM (9 years, 4 months ago) |
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Quote:
Stromrider said: Who cares 

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