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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: Icesyn]
#19256579 - 12/10/13 05:40 PM (10 years, 1 month ago) |
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That's what we had been arguing throughout this whole thread, agar is not hard. Once you dip your toe into the agar pool its kinda hard to stop. Guaranteed that any "noob" who finds a decent clone or isolate is gonna be making slants a week later.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19256581 - 12/10/13 05:41 PM (10 years, 1 month ago) |
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agar..my favorite part of the job
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19256590 - 12/10/13 05:42 PM (10 years, 1 month ago) |
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Quote:
cronicr said: agar..my favorite part of the job
QFT
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blueconfusion
Strangest


Registered: 12/14/12
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Re: Just say NO to LC and YES to PDYA [Re: Icesyn]
#19256731 - 12/10/13 06:10 PM (10 years, 1 month ago) |
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Quote:
Icesyn said: You made agar look like a noob-friendly tek...
You totally should rename this "The Potato Jello Tek"

i seriously was rofl had to show my wife that one she busted up laughing!!!
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19256843 - 12/10/13 06:29 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Quote:
Icesyn said: You made agar look like a noob-friendly tek...
You totally should rename this "The Potato Jello Tek"

i seriously was rofl had to show my wife that one she busted up laughing!!!
someone used yams, so in theory i bet it would be edible and might not taste bad
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Icesyn
Trust My Words


Registered: 05/19/11
Posts: 1,880
Last seen: 5 years, 6 months
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19257707 - 12/10/13 09:18 PM (10 years, 1 month ago) |
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Quote:
twistedty said:
Quote:
blueconfusion said:
Quote:
Icesyn said: You made agar look like a noob-friendly tek...
You totally should rename this "The Potato Jello Tek"

i seriously was rofl had to show my wife that one she busted up laughing!!!
someone used yams, so in theory i bet it would be edible and might not taste bad
Sweet Potato Jello Tek?
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blueconfusion
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Registered: 12/14/12
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Re: Just say NO to LC and YES to PDYA [Re: Icesyn]
#19259428 - 12/11/13 10:14 AM (10 years, 1 month ago) |
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I plan on finishing everything on Friday should be getting all my plates tomorrow but I won't have time basically just reheating agar and pouring. Which will also be posted on the grow along.
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mushrume man
Sadis Factory



Registered: 11/18/08
Posts: 375
Loc: PNW
Last seen: 2 years, 17 days
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19266563 - 12/12/13 04:05 PM (10 years, 1 month ago) |
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Here is an interesting link that I found the other day, it's basically the same as the PYDA recipe in the OP.
Quote:
SpitballJedi said: I haven't had any luck with prints. I've only tried germinating P.Galindoi. I have tried 4 different ways without any luck.
WTF I must be lucky. I have had every spore germinate I've put to agar. Some took weeks but eventually grew just fine. Shiitake was especially slow
Quote:
blueconfusion said:...24 cakes and a plate or two of agar from one syringe... and i don't need syrup water to do that i just use some distilled water. I shoot a few cakes up and then suck up some distilled water shake and keep going.
Are you talking about stretching out a spore syringe by topping up w distilled water between injections?
Just have to say that agar is the best thing for mycology since sliced bread. I love how it keeps things going in the down time and it is real easy to prep. The best part is that its a great reflection of your sterile procedures with quick results.
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: mushrume man]
#19269634 - 12/13/13 07:26 AM (10 years, 1 month ago) |
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 gee is this a contamination or is it mycelium guess i'll have to knock up some test jars to find out , this one i made from a leftover stray grain in my glovebox one transfer later looking clean and sectoring beautifully, tell me how you would/could see this in an LC? by the time this is done i'll be able to knock up several jars and G2G those without any test jars, or worry of is this contamination or mycelium. i can easily make slants from this for long term storage if i so choose as well, which is what i'll probably do. i'll finish up the pics today as soon as i get some time.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270273 - 12/13/13 11:14 AM (10 years, 1 month ago) |
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ive said it before and ill say it again LC is an outdated tek. great work
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19270392 - 12/13/13 11:43 AM (10 years, 1 month ago) |
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 25 dishes poured!!! took a little over 500mL, but i poured a little thick.
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twistedty
Forcefully Retired



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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270399 - 12/13/13 11:45 AM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
 25 dishes poured!!! took a little over 500mL, but i poured a little thick.
just like i like my women.
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blueconfusion
Strangest


Registered: 12/14/12
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
#19270415 - 12/13/13 11:49 AM (10 years, 1 month ago) |
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booty baby got the thickness can i get a witness shake it like she's fearless can i get a witness HELL YEAH!!! sorry puscifer instantly popped in my head 
Edited by blueconfusion (12/13/13 01:08 PM)
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sharpshroomer98
Student of the Universe.



Registered: 08/30/11
Posts: 252
Last seen: 11 days, 18 hours
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270685 - 12/13/13 01:06 PM (10 years, 1 month ago) |
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So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
-------------------- No, I wasn’t born in 98’
 
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blueconfusion
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Registered: 12/14/12
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Quote:
sharpshroomer98 said: So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
basically
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270718 - 12/13/13 01:13 PM (10 years, 1 month ago) |
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Nice going blue! I like your new threads, cool to follow a start to finish grow with all the Q's and comments that goes along
+5
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sharpshroomer98
Student of the Universe.



Registered: 08/30/11
Posts: 252
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270725 - 12/13/13 01:16 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Quote:
sharpshroomer98 said: So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
basically
Well I'm glad this works because I was going to have to do the same thing. Whiskey bottles be too big for my modest PC lol.
Great write up man, and can't wait for more updates
-------------------- No, I wasn’t born in 98’
 
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blueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: spacechildo]
#19270751 - 12/13/13 01:26 PM (10 years, 1 month ago) |
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Quote:
spacechildo said: Nice going blue! I like your new threads, cool to follow a start to finish grow with all the Q's and comments that goes along
+5 
thanks i've written a lot of SOP's
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lessismore
Registered: 02/10/13
Posts: 6,268
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Quote:
sharpshroomer98 said:
Quote:
blueconfusion said:
Quote:
sharpshroomer98 said: So BC, do you just divide the agar up into the smaller bottles because unlike RR not all of us can fit a whiskey bottle in our PC's haha?
basically
Well I'm glad this works because I was going to have to do the same thing. Whiskey bottles be too big for my modest PC lol.
Soya sauce glass bottles work great, they even come with that "wine" fitting making it easier to pour, but usually only around 300mL (500mL is more ideal IMO)
also perhaps small shaker bottles, they are usually microwave safe too for re-heating
and they are usually up to 500-700ml, should work for PCing too with the lid losened a bit I think
agar is easy to sterilize like any liquid
haven't experimented with microwave though... but it might work and make the job easier it will only be a short testing I want to try one day.. I still only trust my PC
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19270870 - 12/13/13 01:52 PM (10 years, 1 month ago) |
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Quote:
blueconfusion said:
Quote:
spacechildo said: Nice going blue! I like your new threads, cool to follow a start to finish grow with all the Q's and comments that goes along
+5 
thanks i've written a lot of SOP's 
Isn't that what it's all about? Reinventing the teks as everyone use different tools and whatnots. Maybe even rephrase some words to give some of us a "aha" moment. Much more interesting than the everyday mono or pf-tek trouble to me.
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