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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19254408 - 12/10/13 08:56 AM (10 years, 1 month ago)

I fucking love to eyeball shit with a pinch of this and a dash of that :lol: Can't wait to see your results. I'm thinking of doing the same thing really. Looking at getting it going tomorrow maybe. Just trying to decide what type of agar to go with. Might do MEA, i haven't made any of that for a while.


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19254438 - 12/10/13 09:11 AM (10 years, 1 month ago)

i'm gonna purposely inoculate a plate or two with some bacteria to see if this is really a viable solution for bacterial prevention.  jars are cooling now!


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19254445 - 12/10/13 09:16 AM (10 years, 1 month ago)


this is a pretty nasty one, gonna take a little of the bacteria and a little chunk of the mycelium


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19254869 - 12/10/13 11:14 AM (10 years, 1 month ago)

just a word of caution pastye don't let your agar cool for more than two hours before adding the tetracycline, i was so worried about thermal degradation of the antibiotic i let the jars almost solidify and mixing the tetracycline in almost became impossible so i have them warming up slightly on the stove to see if i can get it to mix better :lol:


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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19254891 - 12/10/13 11:20 AM (10 years, 1 month ago)

Noted :lmafo:


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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19254936 - 12/10/13 11:34 AM (10 years, 1 month ago)

my LC died after 3-4 months in the refridgerator, in the in the vegetable room

sucks, and not first time it has happened

wonder how to avoid that... it would probably have survived at roomtemp
a syringe I took from it had survived that long in roomtemp

should LCs not be placed in the refridgerator?

how long can LC be stored at roomtemp / fridge?

(btw I used honey LC, but it had plenty of nutrients left I think..)

and if my LC doesnt die it starts to get bacteria in somehow magically after a few months...
bacteria love honey water it seems

might try out this tek :-) , potatoes and agar and corn syrup is easy to find


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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: lessismore]
    #19254950 - 12/10/13 11:37 AM (10 years, 1 month ago)

For long term storage of a culture nothing beats a slant :shrug:


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: lessismore]
    #19255010 - 12/10/13 11:49 AM (10 years, 1 month ago)

Quote:

mio said:
my LC died after 3-4 months in the refridgerator, in the in the vegetable room

sucks, and not first time it has happened

wonder how to avoid that... it would probably have survived at roomtemp
a syringe I took from it had survived that long in roomtemp

should LCs not be placed in the refridgerator?

how long can LC be stored at roomtemp / fridge?

(btw I used honey LC, but it had plenty of nutrients left I think..)

and if my LC doesnt die it starts to get bacteria in somehow magically after a few months...
bacteria love honey water it seems

might try out this tek :-) , potatoes and agar and corn syrup is easy to find



avoid LC dying by not doing them :shrug: like pasty said long term storage is best done in a culture slant.


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19255128 - 12/10/13 12:16 PM (10 years, 1 month ago)

poured 8 plates 4 a little heavier only slightly on the tetracycline gonna do two plates with the known contaminated bacteria and mycelium, two with nothing on them just plates to see if anything decides to pop up without interference two with just bacteria and two with good mycelium.  i know this is way off topic from my original thread but i just wanted to share.


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19255173 - 12/10/13 12:25 PM (10 years, 1 month ago)

Quote:

blueconfusion said:
just a word of caution pastye don't let your agar cool for more than two hours before adding the tetracycline, i was so worried about thermal degradation of the antibiotic i let the jars almost solidify and mixing the tetracycline in almost became impossible so i have them warming up slightly on the stove to see if i can get it to mix better :lol:





wug, ive done this numerous times and it takes forever to melt back down

:lmao:

let us know how the swab of bacteria goes on the antibiotic mix


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19255185 - 12/10/13 12:27 PM (10 years, 1 month ago)

another fun one to do is to take some metabolites from a grain jar(you can just stress them with heat over a few days)suck them up in a syringe and drop them on top of the bacteria on agar


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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
    #19255904 - 12/10/13 03:20 PM (10 years, 1 month ago)

since i put this out there and i'm getting new prints sometime this week as well as my new dishes and cloning tools i'm thinking about starting a grow a long "spore print to spore print" a full start to finish to start grow!  anyone else down?


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19255941 - 12/10/13 03:29 PM (10 years, 1 month ago)

i'm down:thumbup:


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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
    #19255944 - 12/10/13 03:30 PM (10 years, 1 month ago)

Cool it'll be my first time working with prints!  I'll start working on getting the thread setup tonight.  I'm gonna try to leave it open for different techniques unless it's just total BS


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19255969 - 12/10/13 03:38 PM (10 years, 1 month ago)

Quote:

blueconfusion said:
Cool it'll be my first time working with prints!  I'll start working on getting the thread setup tonight.  I'm gonna try to leave it open for different techniques unless it's just total BS





just do itttt

its fun


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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19256007 - 12/10/13 03:45 PM (10 years, 1 month ago)

I haven't had any luck with prints. I've only tried germinating P.Galindoi. I have tried 4 different ways without any luck.


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
    #19256019 - 12/10/13 03:48 PM (10 years, 1 month ago)

i'm getting some galindoi as well but i think i'm gonna try focusing on cubes, i hope i have some luck it would suck to start a grow along and get stuck not being able to get the spore prints to germinate :frown:


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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19256373 - 12/10/13 05:03 PM (10 years, 1 month ago)

I have always had fantastic success with prints to agar. The I got a galindoi print from a sponsor and it would not germinate, and I tried many things. Well I emailed him saying the print was not viable and he replied back saying that they had a bad batch of prints that would not germinate but would send me a replacement. Then it was suggested that in the meantime i make a spore syringe to hydrate my spores and for me to use that to inoculate my agar. Shit i almost fell over cause i was real careful to word my email so that it did not explicitly sound like i was growing. Then i get a response suggesting ways for me to grow them :lol: Great vendor.


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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19256487 - 12/10/13 05:25 PM (10 years, 1 month ago)

Quote:

Pastywhyte said:
I have always had fantastic success with prints to agar. The I got a galindoi print from a sponsor and it would not germinate, and I tried many things. Well I emailed him saying the print was not viable and he replied back saying that they had a bad batch of prints that would not germinate but would send me a replacement. Then it was suggested that in the meantime i make a spore syringe to hydrate my spores and for me to use that to inoculate my agar. Shit i almost fell over cause i was real careful to word my email so that it did not explicitly sound like i was growing. Then i get a response suggesting ways for me to grow them :lol: Great vendor.





lolll. my first pe prints didnt germinate but the ones i have now did and are cased and fruiting.  i would hydrate your spores for best luck


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19256535 - 12/10/13 05:32 PM (10 years, 1 month ago)

You made agar look like a noob-friendly tek...

You totally should rename this "The Potato Jello Tek"
:wtfsonic:


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