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Offlineloki44
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
    #19252754 - 12/09/13 09:36 PM (10 years, 1 month ago)

Quote:

SpitballJedi said:
https://www.shroomery.org/forums/showflat.php/Number/19228936#19228936
Quote:

loki44 said:
I have done 2 pf-tek grows




https://www.shroomery.org/forums/showflat.php/Number/19140900#19140900
Quote:

loki44 said:
hi guys, this is my first ever grain jar




You don't have enough experience to have a valid opinion. Your lack of experience is why you think the way you do.

It's bad form to come on someones thread to criticize when your lack of experience means you are talking complete shit.

You can't establish a reliable pattern for success or growth rate in 2 PF grows and 1 spawn run

Quote:

blueconfusion said:
And I'm not shoving anything down anyone's throat I'm just providing information you were the one that came on to my thread and started running your mouth.




Exactly



if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no

why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil :tongue2: not addressing your post particularly but the tone of several posters


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Offlineloki44
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19252776 - 12/09/13 09:40 PM (10 years, 1 month ago)

Quote:

blueconfusion said:
And I'm not shoving anything down anyone's throat I'm just providing information you were the one that came on to my thread and started running your mouth.




all the info you provided is awesome, you are  a great poster. I just felt I needed to say something about the "say NO to LC" not trying to take anything about your agar procedure, but I dont hink theres need to shit on another tek, my 2 cents


Edited by loki44 (12/09/13 09:41 PM)


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Offlinetwistedty
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
    #19252780 - 12/09/13 09:41 PM (10 years, 1 month ago)

Quote:

loki44 said:

Exactly



if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no

why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil :tongue2: not addressing your post particularly but the tone of several posters





agreed. i pulled off the bucket tek 10x with 0 problems when i was a newb. then i was like well im gonna do 20 tubs.

guess what 15 tubs got trich :frown:.

like i said we want everyone on here to have utmost success and teach others the same methods.

proper pasteurization is #1 and then agar is tied.

no hard feeling bro, if i came to your house id definetly eat your glc boomers with no complaints :wink:


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Offlinetwistedty
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
    #19252802 - 12/09/13 09:45 PM (10 years, 1 month ago)

Quote:

loki44 said:

all the info you provided is awesome, you are  a great poster. I just felt I needed to say something about the "say NO to LC" not trying to take anything about your agar procedure, but I dont hink theres need to shit on another tek, my 2 cents





the bucket tek works too. any EXPERIENCED grower will not reccomend it either.


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Offlineloki44
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19252847 - 12/09/13 09:52 PM (10 years, 1 month ago)

Quote:

twistedty said:
Quote:

loki44 said:

Exactly



if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no

why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil :tongue2: not addressing your post particularly but the tone of several posters





agreed. i pulled off the bucket tek 10x with 0 problems when i was a newb. then i was like well im gonna do 20 tubs.

guess what 15 tubs got trich :frown:.

like i said we want everyone on here to have utmost success and teach others the same methods.

proper pasteurization is #1 and then agar is tied.

no hard feeling bro, if i came to your house id definetly eat your glc boomers with no complaints :wink:




haha,no hard feeligs either, but I cant bash something I have had no contams, only when a PATTERN of contamination arises then you can say ok, there is a problem, don't be too quick to blame your spawn, could be you didn't properly pasteurized, or your substrate wasn't at field capacity etc not talking about you in particular, just generally speaking


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InvisibleSpitballJedi
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
    #19252855 - 12/09/13 09:54 PM (10 years, 1 month ago)

Quote:

loki44 said:
if a total inexperienced noob can pull off a clean GLC twice, the odds should say it would be contaminated right? hmmm no

why people get so buthurt and bent out of shape if i dare to say something against the "agar dogma' holy shit people that do pshycs shouldn't be so dogmatic and negative...relax is just a GLC not the devil :tongue2: not addressing your post particularly but the tone of several posters




Keep doing it. But please make a new thread with your results over time.

It's hard to say why shitty practices work sometimes. In my line of work, I'm amazed at the things that work sometimes when they really shouldn't be because usually they don't.

Many people have made successful GLC. But don't get dependent on it because it's unreliable. You can't see this yet because you have not been doing it enough.

Some people are dogmatic about stuff and get upset when you disagree. Nobody's butthurt here, I just think you're being silly. There is a difference between dogma and real experience.


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Offlinetwistedty
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Re: Just say NO to LC and YES to PDYA [Re: loki44]
    #19252859 - 12/09/13 09:55 PM (10 years, 1 month ago)

nah my spawn is on point :wink:. bucket tek was the problem fo show


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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19252886 - 12/09/13 10:01 PM (10 years, 1 month ago)

I found myself blaming the bucket for some of my problems, namely verticillium which I saw more than trich for sure. As verticillium is supposed to only be introduced during spawning I figured that was my vector and abandoned the bucket. Since starting proper pasteurization I only saw verticillium once and it was a mild case, but funny enough, I have been starting to sterilize my coir verm and I thought for sure the dry bubble would come back at that point, however I have yet to see it :shrug: Gonna do a whole shitload more sterilization projects in the future tho just to be sure.


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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19252939 - 12/09/13 10:10 PM (10 years, 1 month ago)

Quote:

Pastywhyte said:
I found myself blaming the bucket for some of my problems, namely verticillium which I saw more than trich for sure. As verticillium is supposed to only be introduced during spawning I figured that was my vector and abandoned the bucket. Since starting proper pasteurization I only saw verticillium once and it was a mild case, but funny enough, I have been starting to sterilize my coir verm and I thought for sure the dry bubble would come back at that point, however I have yet to see it :shrug: Gonna do a whole shitload more sterilization projects in the future tho just to be sure.




youre sterilizing your coir/verm?  never had vert myself only trich from the bucket.  and a mild green mold from some mold hidden in my agar that would show up during/after first flush but didnt do anything negative to my harvest.

was able to flush it out np but threw it away anyway.

got rid of those cultures and havent had any probs since :shrug:

its always good to be able to diagnose the problem.

frank had the same problem as well during the same time, a lil collaboration and he thought it was the agar as well.


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InvisiblePastywhyteMDiscord
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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19253022 - 12/09/13 10:32 PM (10 years, 1 month ago)

Quote:

twistedty said:
youre sterilizing your coir/verm?



Sure am :lol: Here is the link. First flush is already in the can and one of the tubs is showing the second already.



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Offlinetwistedty
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19253027 - 12/09/13 10:34 PM (10 years, 1 month ago)

Quote:

Pastywhyte said:
Quote:

twistedty said:
youre sterilizing your coir/verm?



Sure am :lol: Here is the link. First flush is already in the can and one of the tubs is showing the second already.








:yay:



roadhouse.  you autoclave or.....


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19253031 - 12/09/13 10:34 PM (10 years, 1 month ago)

nm i clicked the link :stoned:


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19253040 - 12/09/13 10:37 PM (10 years, 1 month ago)

well shit,im ALWAYS in my kitchen my stovetop only holds 2 pcs and thats only 14 qts and i have to do 28 a day damn near for 3 tubs.

its like an 8 hr job.

might give this a shot fo show


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Re: Just say NO to LC and YES to PDYA [Re: twistedty]
    #19253046 - 12/09/13 10:39 PM (10 years, 1 month ago)

Get in on the action, everyone is welcome :thumbup: I believe Spitball is doing a tub and I think Frank is up for it as well :awesomenod:


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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19253992 - 12/10/13 05:34 AM (10 years, 1 month ago)

I should be putting mine in fruitin in the next day or two


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
    #19254034 - 12/10/13 06:01 AM (10 years, 1 month ago)

@Loki i didn't mean for the "Just say NO to LC" to come off as i was bashing any one tek, i had success with my LC; for a while...  I was all about LC's for about 4 months 0 problems the same with the bucket tek, the bucket tek is great i've had success with that as well.  i work 60 hours a week so the "easy way" was the most appealing for me, until i realized the potential i was missing out on, the actual science of this hobby.  i am by no means "a commercial" grower as you have called us, and i don't have lots of money to waste this is why i'm trying to help others who want to know how to make the most of the dollar they spend.  i do pf cakes too, but what i do is just a drop on my agar (which is more than enough) and then inoculate some brf cakes; in fact i have 12 cakes fruiting right now.  i have also found ways to stretch that syringe out i can actually make somewhere around 24 cakes and a plate or two of agar from one syringe...  and i don't need syrup water to do that i just use some distilled water.  i shoot a few cakes up and then suck up some distilled water shake and keep going.


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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
    #19254081 - 12/10/13 06:24 AM (10 years, 1 month ago)

Quote:

SpitballJedi said:
I should be putting mine in fruitin in the next day or two



:fuckinawesome:


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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
    #19254093 - 12/10/13 06:32 AM (10 years, 1 month ago)

i have a little extra time today, actually taking a day off work so i might get started on my antibiotic agar again ROUND 2; this time I'll be using MEA just cause I have all the stuff to do it, and i don't wanna boil potatoes :lol:...  the mix will most likely be 9 grams of Malt Extract and 9 grams of Agar, in 500 mL. water.  As for the tetracycline probably only add maybe a 1/2 tsp to one jar and a full tsp. to the other once they have cooled enough. 
gonna prep pretty much same way as PDYA simmer ME in 500 mL. of water until it has dissolves remove from heat add agar, then PC let cool for about an hour to 1.5 hours...  i'm probably gonna pour this in old plates and use a grain as spawn.  i'll post results as i go.


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19254202 - 12/10/13 07:31 AM (10 years, 1 month ago)

had just enough agar to do this experiment (antibiotic w/ tetracycline).  hoping it turns out just put my vessels in the PC to get this going.:super: after all the debate last night maybe i should have not posted this thread as "just say no to lc and yes to pdya", but rather "can you make jello then try agar", thanks twisted where were you when i was creating this thread :lol: i love that quote it's so true!
gonna change how much tetracycline i add as well.  i'll let everyone know as soon as i get to that point


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Edited by blueconfusion (12/10/13 07:37 AM)


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Offlineblueconfusion
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
    #19254385 - 12/10/13 08:49 AM (10 years, 1 month ago)

ok the scientific side of this experiment just went out the window i just realized my friend has my scales to measure anything small so i'm eyeballing it (he's outta town for a while) so here is my eyeballing

:shrug:
if i can get something outta this i'll get some more precise measurements but those are tsps. with about a knife tip of tetracycline on each tsp.


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