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mermus
Nerf Hearder



Registered: 07/17/13
Posts: 127
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rye issues
#19248233 - 12/09/13 12:34 AM (10 years, 1 month ago) |
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These have been knocked up around 10 days now, smell fine, but all have this strange goo in them, any suggestions mates?
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: rye issues [Re: mermus]
#19248290 - 12/09/13 12:54 AM (10 years, 1 month ago) |
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Heavy bacterial contamination 
What's up with your procedures?
My first question would be how you prepared the grain.
Second would concern your inoculation method.
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sharpshroomer98
Student of the Universe.



Registered: 08/30/11
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They look like they might've been too wet after the prep. Any pics of the prep itself?
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silverstem
Caps & Stems



Registered: 10/12/13
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toss them and try again... what type of ge filter are you using? why is there foil on top?
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wildernessjunkie
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Registered: 06/13/10
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Bacteria for sure.
It sucks, but shit happens. There's no saving those. toss them out and try again.
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mermus
Nerf Hearder



Registered: 07/17/13
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http://www.shroomery.org/forums/showflat.php/Number/6379224#6379224
Followed this tek to the letter. Had success before but now the liquid. Literally followed Magesh's tek to the letter with the ezception of using a syringe bought from thehawkseye which I have used many times before and have known others to use. Knocked them up thru a tyvek filter put the foil over the tyvek and put them in the incub
Edited by mermus (12/09/13 01:03 PM)
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wildernessjunkie
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Re: rye issues [Re: mermus]
#19250157 - 12/09/13 01:30 PM (10 years, 1 month ago) |
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The bacteria isn't a matter of grain preparation.
Somewhere along the line, the contamination was introduced.
Edit: Though if your grain is too wet, it will contribute to any introduced or already present bacteria.
Edited by wildernessjunkie (12/09/13 03:44 PM)
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mermus
Nerf Hearder



Registered: 07/17/13
Posts: 127
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Re: rye issues [Re: mermus]
#19251502 - 12/09/13 05:53 PM (10 years, 1 month ago) |
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I inject thru a hole in the lid covored in tyvek then out foil over the top of the jar and incubate. All surfaces and the air are sterilized well
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SpitballJedi
Ancient Astronaut



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Re: rye issues [Re: mermus]
#19251655 - 12/09/13 06:17 PM (10 years, 1 month ago) |
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So, you have a hole in the tyvek when you're done inoculating? If so, that's likely the problem.
The surface is not sterile once it is in open air. If you didn't flame sterilize your needle, then you introduced contamination that way. If there is a hole in your tyvek, the contamiation could have gotten in at any point in time.
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twistedty
Forcefully Retired



Registered: 07/01/12
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when i used syringes i used polyfil, inject right threw it, no need for foil on your jars they need to breath
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Quote:
SpitballJedi said: So, you have a hole in the tyvek when you're done inoculating? If so, that's likely the problem.
The surface is not sterile once it is in open air. If you didn't flame sterilize your needle, then you introduced contamination that way. If there is a hole in your tyvek, the contamiation could have gotten in at any point in time.
... use polyfill or SFD's for your lids. If you gotta use tyvek, atleast cover the hole with a breathable tape 
@Wildernessjunkie
If you don't think you can get bacteria from how you prep your grain... try PCing straight out of the soak water.
Oh wait you made an edit
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TheMustardTiger


Registered: 08/19/13
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http://www.shroomery.org/forums/showflat.php/Number/14722818#14722818
These grain lids work like a charm. I personally use the higher end self healing injection ports for inoculating grain with MS instead of the blob of silicone but both work great. Follow that lid tek and get ready for success.
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mermus
Nerf Hearder



Registered: 07/17/13
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I flame the needle every single injection. I used to put bandaids over the injection holes but got told itwas pointless. I know nothing of polyfill
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wildernessjunkie
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Re: rye issues [Re: mermus]
#19253357 - 12/10/13 12:04 AM (10 years, 1 month ago) |
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Quote:
If you don't think you can get bacteria from how you prep your grain... try PCing straight out of the soak water.
Yes. I edited. But Ill still disagree on this point.
When you PC at 15 psi for 90-120 minutes, it should kill everything in there. While there are a few things that can survive such temps, they are few and far between.
Assuming proper sterilization procedures, the primary cause of contamination is an introduction by the person inoculating the grain/substrate/agar/whatever else.
This is especially true if working with spores. Spores are not sterile. Aseptic, yes. Clean, maybe. But one thing is for sure, they are definitely a contamination vector.
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
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Re: rye issues [Re: mermus]
#19253382 - 12/10/13 12:10 AM (10 years, 1 month ago) |
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Quote:
mermus said: I know nothing of polyfill
That's hillarious! But seriously, it's just pillow stuffing.
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mermus
Nerf Hearder



Registered: 07/17/13
Posts: 127
Loc: USA
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Glad I could give ya a laugh friend, when i read polyfill i thought "maybe it is something like FA or RH another term for something" i worked in a pillow factory for a few years as a teenager, I know that kind of polyfill, still glad ya got a laugh from it
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
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Re: rye issues [Re: mermus]
#19253397 - 12/10/13 12:14 AM (10 years, 1 month ago) |
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mermus
Nerf Hearder



Registered: 07/17/13
Posts: 127
Loc: USA
Last seen: 7 years, 11 months
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Re: rye issues [Re: mermus]
#19253403 - 12/10/13 12:17 AM (10 years, 1 month ago) |
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I was putting foil on because I have always been under the impression it was a standard thing no matter what, plus I have tons of the stuff everywhere, i always PC it on the jars. Also I was always taught to just cut a tyvek envelope from the USPS to fit the jar lid and and inoculate thru it and put a bandaid over the knock up hole, like i said earlier i got told i was pissing away money and time with the bandaid.
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: rye issues [Re: mermus]
#19253424 - 12/10/13 12:23 AM (10 years, 1 month ago) |
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Heard.
It's always a good idea to put foil over your jars when you PC. That way your filter won't get wet & wick contams in your jar.
I've never used tyvek as a jar filter personally. If I was in your shoes, I'd go buy a cheap pillow and use the shit out of there as your GE filter.
I like the SFD's cause I don't have to change them every time I do a new spawn run, but they are a little pricey. Def worth it IMO
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mermus
Nerf Hearder



Registered: 07/17/13
Posts: 127
Loc: USA
Last seen: 7 years, 11 months
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i am seriously considering the SFD's but how do i even use pollyfill?
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: rye issues [Re: mermus]
#19253472 - 12/10/13 12:42 AM (10 years, 1 month ago) |
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Take a wad a little smaller than in my pic and stuff it through a pre-drilled hole in your lid.
Any size hole from 1/8-1/4 inch should be fine
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twistedty
Forcefully Retired



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Re: rye issues [Re: mermus]
#19253709 - 12/10/13 02:14 AM (10 years, 1 month ago) |
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Quote:
mermus said: i am seriously considering the SFD's but how do i even use pollyfill?
drill 1/4 in hole into a lid and snugly pack it. just be careful noccing threw it with syringe you want it tight enough where the syringe wont push it in the jar.
also sfds are the best filters you can get.
highly recommend if youre gonna stick with this hobby
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sharpshroomer98
Student of the Universe.



Registered: 08/30/11
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-------------------- No, I wasn’t born in 98’
 
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mermus
Nerf Hearder



Registered: 07/17/13
Posts: 127
Loc: USA
Last seen: 7 years, 11 months
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You guys have been so helpful. I made the leap from BRF cakes to rye and casings. Guess I was very ill informed on my leap thanks guys
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newera
Stranger


Registered: 03/05/13
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Re: rye issues [Re: mermus]
#19255494 - 12/10/13 01:42 PM (10 years, 1 month ago) |
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drill a hole, stuff it tightly
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twistedty
Forcefully Retired



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Re: rye issues [Re: newera]
#19255502 - 12/10/13 01:45 PM (10 years, 1 month ago) |
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Quote:
newera said: drill a hole, stuff it tightly
thats what she said.
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mermus
Nerf Hearder



Registered: 07/17/13
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wouldnt using silicone clog up the end of a syringe? or am i just over thinking that?
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
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Re: rye issues [Re: mermus]
#19270103 - 12/13/13 10:24 AM (10 years, 1 month ago) |
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RTV silicone ports work pretttttty good.
I like injecting through them much better than polyfill.
I only have about 10 lids with an extra hole for syringe inoculations though...
G2G doesn't need a needle
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PocketRevolution
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Regular old Kitchen & Bath 100% silicone works fine for injection ports too. Plus, you can see through it to identify where the hole in a jar lid is.
Tyvek makes a fine filter material, but you should not inject through it. Either remove the lid and squirt the solution into the jar, or build a lid with some type of injection port (polyfill, silicone, etc).
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mermus
Nerf Hearder



Registered: 07/17/13
Posts: 127
Loc: USA
Last seen: 7 years, 11 months
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You guys have been very helpful in this. Thank you all.
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