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Offlinegratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
first time inoculating... questions
    #19247292 - 12/08/13 09:16 PM (10 years, 1 month ago)

so its my first time inoculating.
I PC'd my brf cakes last night,gonna pull them out in a couple hours to inoculate them, and i have a question about transferring my sterile jars into my lab.

I have my steroclave is in my kitchen, which is a living room plus a hall walk away from my sterile flow hood lab room... how do you suggest i bring my jars into my lab, should i just open my steroclave in my kitchen and walk them back to my lab, turn on the flow hood and return in a hour to work, or just pick up the steroclave and walk that back to my room, wipe the clave down run the flow hood for a hour and then open them while the room is in ready and working order?

thanks for the help


--------------------
Everybody got their own trip as you walk down the strip
Your life could be changed by a talk with a stranger
Someone told me a story they said that he knew me
He told me a rhyme that transcended all time
How we shared the same space, another time, another place
He spoke with a rush about how so many lives have been touched


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
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Re: first time inoculating... questions [Re: gratefulcheese]
    #19247303 - 12/08/13 09:18 PM (10 years, 1 month ago)

Run the hood for an hour or 2, then carry the jars to the hood.

Remove the foil and keep it off, do not put it back on.

Wipe the jars with 70% alcohol and place in front of the hood.

You should have sanitized gloves on when doing this.....


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Offlinegratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
Re: first time inoculating... questions [Re: PussyFart]
    #19247408 - 12/08/13 09:36 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Run the hood for an hour or 2, then carry the jars to the hood.

Remove the foil and keep it off, do not put it back on.

Wipe the jars with 70% alcohol and place in front of the hood.

You should have sanitized gloves on when doing this.....




so are you suggesting, turn on the hoods, wait an hour +, shower, throw on scrubs, then go from my shower in my scrubs back to the kitchen, and carry everything back to my lab and begin work?

I feel like all the work of cleaning my self off in the shower goes out the window if I have to walk through my entire house and gather whatever contaminations and drag them back to my clean lab.

but im the one posing the question, so tell me whatever you think is best


--------------------
Everybody got their own trip as you walk down the strip
Your life could be changed by a talk with a stranger
Someone told me a story they said that he knew me
He told me a rhyme that transcended all time
How we shared the same space, another time, another place
He spoke with a rush about how so many lives have been touched


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
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Re: first time inoculating... questions [Re: gratefulcheese]
    #19247432 - 12/08/13 09:42 PM (10 years, 1 month ago)

I never shower before I work......I just wear gloves and tyvek sleeves sprayed down with 70% alcohol......And I have a pretty good success rate in my SAB.

All that matters is the immediate work area......and what will be in the sterile air flow.


Edited by PussyFart (12/08/13 09:43 PM)


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InvisibleDelection
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Registered: 10/08/11
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Re: first time inoculating... questions [Re: gratefulcheese]
    #19247435 - 12/08/13 09:43 PM (10 years, 1 month ago)

Most people don't even have autoclaves when starting off with the PF Tek method, let alone flow hoods.. The top layer or dry verm also acts as a barrier against contamination on top of the tinfoil.. I think your going to be ok :cool:


--------------------
Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”


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OfflineDannyDGAF
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Re: first time inoculating... questions [Re: Delection]
    #19247457 - 12/08/13 09:47 PM (10 years, 1 month ago)

Quote:

Delection said:
The top layer or dry verm also acts as a barrier against contamination on top of the tinfoil..




You take tinfoil off after you take the jars out of the pot or whatever your using.  It's only used to keep the water from the pot that's dripping from the lid out of the substrate.


--------------------
"You tell him, and I will smack you. I will smack you like a bad, bad donkey!"


Our dreams are a second life. I have never been able to penetrate without a shudder those ivory or horned gates which separate us from the invisible world.


"In order to use your head, you have to go out of your mind" -  Tim Leary


Edited by DannyDGAF (12/08/13 09:48 PM)


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,810
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Re: first time inoculating... questions [Re: DannyDGAF]
    #19247488 - 12/08/13 09:54 PM (10 years, 1 month ago)

You have a flow hood and an autoclave! And your doing pf tek??? Shit man, this might be your first ride but ya just filled up that Ferrari with diesel :lol: All kidding aside I personally feel that the reason most noobs are encouraged to start with pf tek is because most of them don't wanna spring for the cost of a PC :shrug: If you ain't doing agar and grains in two months something is wrong :lmafo:


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InvisibleDelection
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Re: first time inoculating... questions [Re: DannyDGAF]
    #19247683 - 12/08/13 10:29 PM (10 years, 1 month ago)

Quote:

DannyDGAF said:
Quote:

Delection said:
The top layer or dry verm also acts as a barrier against contamination on top of the tinfoil..




You take tinfoil off after you take the jars out of the pot or whatever your using.  It's only used to keep the water from the pot that's dripping from the lid out of the substrate.




I realize this.. I was only stating that he has two layers of barriers before he gets to his flow hood before inoculation.


--------------------
Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”


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Offlinegratefulcheese
Miss Browns Tea House Patron

Registered: 10/01/13
Posts: 73
Last seen: 10 years, 1 day
Re: first time inoculating... questions [Re: Pastywhyte]
    #19247692 - 12/08/13 10:31 PM (10 years, 1 month ago)

thanks for all the encouragement... Im first timing with the pf tek because I am trying to just do baby steps... Im going for grain the next go around. Besides following RR "lets Grow..." DVD im attempting to follow basic instructions a friend has hand written for me, which suggested starting with the mere basics and working my way up. Thank you all for all the help.
Light and Blessings...


--------------------
Everybody got their own trip as you walk down the strip
Your life could be changed by a talk with a stranger
Someone told me a story they said that he knew me
He told me a rhyme that transcended all time
How we shared the same space, another time, another place
He spoke with a rush about how so many lives have been touched


Edited by gratefulcheese (12/08/13 10:32 PM)


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OfflineDannyDGAF
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Registered: 06/10/13
Posts: 533
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Re: first time inoculating... questions [Re: Delection]
    #19247748 - 12/08/13 10:43 PM (10 years, 1 month ago)

Quote:

Delection said:
I realize this.. I was only stating that he has two layers of barriers before he gets to his flow hood before inoculation.




Gotcha, that makes sense now.


--------------------
"You tell him, and I will smack you. I will smack you like a bad, bad donkey!"


Our dreams are a second life. I have never been able to penetrate without a shudder those ivory or horned gates which separate us from the invisible world.


"In order to use your head, you have to go out of your mind" -  Tim Leary


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