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Invisiblemycomattie
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Registered: 11/15/12
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PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub *UPDATE 12/26 - I Fucked Up*
    #19179385 - 11/23/13 03:28 PM (10 years, 3 months ago)

11/13/13

Earlier this month, I noticed I had a quart of PE on a shelf, fully colonized, since mid September.  It was inoculated with an agar wedge containing a PE isolate.

As you can see in the pic - it's very dehydrated and bruised blue from me trying to beat it/break it up a bit for the grain to gran transfer.



I had 3 empty quart jars available; so I prepped them with Rye Grass Seed (soaked RGS/gypsum/diluted coffee/boiling water for ~ 5 hours) and PC'd at 15psi, for 90 minutes.



I'm just curious if I can get a good grow from what are essentially spare supplies and 1 old quart jar.

11/14/13

Just G2G'd - which sucked, since the mycelium had consolidated to such a point that it was breaking off in large chunks (which is why G2G is recommended at exactly 100% colonization).  I used a disposable scalpel that I usually use for agar.

My SAB which isn't fancy, but has been pretty reliable.



The jars after inoculated.



II'm hoping I can get a good grow from what are essentially spare supplies and 1 old quart jar.

We shall see...

11/20/13

Ah the joys of home mycology - patience...

This PE was originally from a MS syringe and it's overall colonization speed is SLOW - even when the rhizomorphic sectors were isolated.



11/23/13

Shook them this morning.  For whatever reason, shaking doesn't seem to work to well with these PE's - the mycelium consolidates and stays chunky, which makes it hard to break up and spread throughout the quart.

The good thing about RGS - it creates thousands of inoculation points.

I'll update as I go :wink:


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Edited by mycomattie (12/26/13 11:54 AM)


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Offlineshaneh79
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19179940 - 11/23/13 06:09 PM (10 years, 3 months ago)

Cool how you can create so much from such small beginnings in musicology. I am working on my first isolation of Mexi cub right now I'm excited! Thanks for posting your grow!


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: shaneh79]
    #19180001 - 11/23/13 06:28 PM (10 years, 3 months ago)

Quote:

shaneh79 said:
Cool how you can create so much from such small beginnings in musicology. I am working on my first isolation of Mexi cub right now I'm excited! Thanks for posting your grow!




Definitely my friend - I'm new to agar/isolation myself, so best wishes and good vibes on your mycological endeavors.


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19187928 - 11/25/13 05:15 PM (10 years, 2 months ago)

11/25/13

Well, It's coming along, but that may be my last shake for this PE - it definitely seems like it may be slowing the colonization; not speeding it up  :facepalm3:

Latest pics...




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Offlinesilverstem
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19188090 - 11/25/13 05:51 PM (10 years, 2 months ago)

you have to allow time for your myc to recover after shaking... it will slow growth but growth speed will increase after recovery.


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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: silverstem]
    #19188193 - 11/25/13 06:11 PM (10 years, 2 months ago)

Quote:

silverstem said:
you have to allow time for your myc to recover after shaking... it will slow growth but growth speed will increase after recovery.




Very true - it's just this PE is slower than anything I've experienced in the past............but, better than the "C" word; at least the mycelium looks healthy.


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Offlinesilverstem
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19188197 - 11/25/13 06:13 PM (10 years, 2 months ago)

yea it looks very healthy...


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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: silverstem]
    #19189370 - 11/25/13 10:14 PM (10 years, 2 months ago)

Quote:

silverstem said:
yea it looks very healthy...




Thanks, hopefully it stays that way haha - I'll take slow colonization over contams though :\


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19204509 - 11/29/13 11:24 AM (10 years, 2 months ago)

11/29/13

Well, the jars are coming along - I'd say, as of today, they're ~ 70 - 80% colonized.  Definitely testing my patience.



On a side note - I have several agar projects going on. 



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Offlinespacechildo
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19204537 - 11/29/13 11:35 AM (10 years, 2 months ago)

shake shake shake?


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: spacechildo]
    #19246809 - 12/08/13 07:37 PM (10 years, 2 months ago)

12/8/13

Spawned to bulk in a monotub.  Actually yesterday, but I took pictures today.



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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19247002 - 12/08/13 08:17 PM (10 years, 2 months ago)

Good stuff mattie :thumbup: I gonna watch this one :awesomenod:


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: Pastywhyte]
    #19249124 - 12/09/13 08:20 AM (10 years, 2 months ago)

Thank you man!


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Invisiblebootster
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19249590 - 12/09/13 11:09 AM (10 years, 2 months ago)

:thumbup:  Good job.


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: bootster]
    #19256689 - 12/10/13 06:01 PM (10 years, 2 months ago)

Thanks everyone.

11/18/13

The monotub is colonizing nicely (yup, I open the lid to check every few days, which I know is considered "bad practice").



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InvisiblePastywhyteMDiscord
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19256710 - 12/10/13 06:05 PM (10 years, 2 months ago)

I don't consider it bad practice, I do it all the time. Nice looking tub BTW.


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OfflinecronicrFacebook
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: Pastywhyte]
    #19256714 - 12/10/13 06:06 PM (10 years, 2 months ago)

everyday is bad practice, every few is good parenting


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: cronicr]
    #19257374 - 12/10/13 08:00 PM (10 years, 2 months ago)

Cool - thanks guys!


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Invisiblemycomattie
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19319985 - 12/24/13 07:48 AM (10 years, 2 months ago)

12/24/13

Fruiting was initiated 10 days ago, and today I see the very first pin set.  I'm curious to see the overall results, since this is my first time casing cubensis.


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OfflinecronicrFacebook
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
    #19320716 - 12/24/13 11:41 AM (10 years, 2 months ago)

Sweet!


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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