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Offlineurthtown
meat popsicle
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Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia Flag
Last seen: 2 years, 4 months
Agar practice
    #19246927 - 12/08/13 07:59 PM (10 years, 1 month ago)

Hello folks. I have a 15.5 qt AA on the way but not yet in my hands but I've cobbled together just about everything else I need to go agar > grain > bulk and am excited about starting some edible and cubes once my AA arrives. In the mean time I have made a few mason jars of agar media just to practice technique on in my SAB and to try to get a feel for working with it in those conditions.

I am practicing here so not really concerned with the cultures I get out of them but wanted to share and ask some questions too. I've just finished making a bunch of grain jar lids and mason jar petri dish lids with SFDs and red silicone:

You can see I put a 1/4" hole with 3 1/16" satellite holes around for each GE hole using them to lock the silicone in place. I did the same for the nocc points.




I made up 4 more jars of agar to practice a transfer on. I also kept a mason jar from each batch, opened it in my SAB as if I was doing a tissue transfer or inoculation but then just put the lid back on to see if my SAB is working well. So far the first one is 2 weeks no con tams. Here are the new batch of MEA.



So now to the questions. I have a clone of a store bought shitake growing on agar. The tissue turned purple and dark after about a day or so but I checked today and it looks like it's recovering. Again not concerned with the viability of this culture - I'll purchase my edible cultures when my PC arrives. Does this look like shitake?



Next was a piece of a BRF cake I was crumbling up - I took it from inside the cake and dipped in 3% peroxide for 30 seconds and then transferred to the agar. Now this I am doubting is myc, again no worries if it isn't but what do you guys think? Learning a lot. Glad I have these practice runs to get my head around everything and test my setup.



Thanks for checking this out - I know it's a bit silly to be dabbling around with this without a PC but I really just want to keep learning about the hobby and have been really enjoying delving into agar work in the SAB. Can't wait to get the wheels turning on the whole setup.

Cheers! (p.s. thanks for all the teks I used to learn about this stuff, especially ProfessorPinHead's no pour agar tek and GungGung's lid tek)


--------------------
Cluster Headache sufferer? Cluster Busting :thumbup:
Veil Tear GIF
Flower Pot Grow GIF
Mini Mono Tub GIFS

"All mushrooms are edible, but some only once."
-- Croatian Proverb



Edited by urthtown (12/08/13 09:46 PM)


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Offlineurthtown
meat popsicle
Male


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia Flag
Last seen: 2 years, 4 months
Re: Agar practice [Re: urthtown]
    #19378031 - 01/06/14 04:34 PM (10 years, 24 days ago)

Well I'm glad I had some practice - none of the above practice amounted to anything but I did two xfers away from a contam on a clone I just made of a nice looking shroom and.... both xfers are colonizing free from contams. Agar is a lot of fun.



This is the dead plate after the xfers.  :cthulhu: :rockets:


--------------------
Cluster Headache sufferer? Cluster Busting :thumbup:
Veil Tear GIF
Flower Pot Grow GIF
Mini Mono Tub GIFS

"All mushrooms are edible, but some only once."
-- Croatian Proverb



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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Agar practice [Re: urthtown]
    #19378052 - 01/06/14 04:40 PM (10 years, 24 days ago)

awesome stuff to hear! glad your having fun with it:thumbup:
get a contam timelapse for kick sakes


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Agar practice [Re: urthtown]
    #19378088 - 01/06/14 04:50 PM (10 years, 24 days ago)

Quote:

urthtown said:
Agar is a lot of fun.




I LOVE them short shouldered jars you have.  When I want jars like that, I can only find the standard wide mouth 1/2 pint and vice versa. 

No-pour agar/Mason jar agar is a great place to start, and depending on the individual more than sufficient. 

I have found through my studies that I have converted from the metal lids to the plastic ones for a few reasons.  One piece construction is less clumsy inside the SAB, and they don't get all stuck fast to the glass jar like the metal lids.  I have bent up a few metal lids in my time just trying to pry them open, and it is hilarious every time.  It only happens on my last jar, and causes screaming......Seems like you figured that one out early :thumbup:

I recently picked up another dozen more jars, but no plastic lids, and started mounting them upside down under the ring.  I have had no issues with contaminants finding their way down to the edges, but I keep agar sequestered in their own colonizing box.  Out of the SAB and right into a 14qt tub with no holes. I like to think that this has saved a few dishes. 

Agar is both fun and educational :smile:.


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Offlineurthtown
meat popsicle
Male


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia Flag
Last seen: 2 years, 4 months
Re: Agar practice [Re: HypnotoadCroaked]
    #19454367 - 01/21/14 06:50 PM (10 years, 9 days ago)

Quote:

HypnotoadCroaked said:
Quote:

urthtown said:
Agar is a lot of fun.




I LOVE them short shouldered jars you have.  When I want jars like that, I can only find the standard wide mouth 1/2 pint and vice versa. 

No-pour agar/Mason jar agar is a great place to start, and depending on the individual more than sufficient. 

I have found through my studies that I have converted from the metal lids to the plastic ones for a few reasons.  One piece construction is less clumsy inside the SAB, and they don't get all stuck fast to the glass jar like the metal lids.  I have bent up a few metal lids in my time just trying to pry them open, and it is hilarious every time.  It only happens on my last jar, and causes screaming......Seems like you figured that one out early :thumbup:

I recently picked up another dozen more jars, but no plastic lids, and started mounting them upside down under the ring.  I have had no issues with contaminants finding their way down to the edges, but I keep agar sequestered in their own colonizing box.  Out of the SAB and right into a 14qt tub with no holes. I like to think that this has saved a few dishes. 

Agar is both fun and educational :smile:.




Nice! Yeah the short-shouldered decorative masons are sweet for agar though I have had two yeast contams that were not readily visible from the viewing angle but hey ya can't make an omelette without breaking a few eggs... eh?  eh?? :shrug:

Just put a shiitake LC I just ordered on agar and nocc'd up some grain spawn, put some P. mexicana spores on agar, a P. cubensis MS swab from a spore print that I took on agar and some P. djamor on agar! Getting tons of practice now haha. Letting the PC cool with the no-pours inside got rid of my condensation and I have a rubber jar grip that I loosen the lids with when I am loading the SAB so everything is rolling smoothly :thumbup:


--------------------
Cluster Headache sufferer? Cluster Busting :thumbup:
Veil Tear GIF
Flower Pot Grow GIF
Mini Mono Tub GIFS

"All mushrooms are edible, but some only once."
-- Croatian Proverb



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