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blueyz



Registered: 11/09/13
Posts: 50
Loc: CO
Last seen: 9 years, 5 months
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Newb Question
#19244837 - 12/08/13 12:49 PM (10 years, 1 month ago) |
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I have a buddy who inoculated some jars according to the PF tek (shitokai of course), but had no growth, good or bad. The only deviation was he sterilized the jars and left them in the PC for almost a week because there was not an ideal time to inoculate. Is that reason enough to stunt the growth? Do you think the substrate dried out too much?
Thanks
-------------------- Peace & Love
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Newb Question [Re: blueyz]
#19245011 - 12/08/13 01:26 PM (10 years, 1 month ago) |
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no it wouldn't have dried out too much
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
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Re: Newb Question [Re: cronicr]
#19245233 - 12/08/13 02:12 PM (10 years, 1 month ago) |
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Was this a MS inoculation? Sounds like contam or just piss poor spores. Give them a smell? Try to to get a cake out (Outside of your grow area, of course) and give it a good inspection for contams or anything else that May have halted or prevented any growth.
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Halophorm
Pinset extrordinaire



Registered: 11/25/13
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Quote:
MustardTiger66 said: Was this a MS inoculation? Sounds like contam or just piss poor spores. Give them a smell? Try to to get a cake out (Outside of your grow area, of course) and give it a good inspection for contams or anything else that May have halted or prevented any growth.
what he said
-------------------- "Imagination is more important than knowledge." -Albert Einstien
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Halophorm
Pinset extrordinaire



Registered: 11/25/13
Posts: 114
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Re: Newb Question [Re: blueyz]
#19245279 - 12/08/13 02:23 PM (10 years, 1 month ago) |
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OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.
-------------------- "Imagination is more important than knowledge." -Albert Einstien
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
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Quote:
Halophorm said: OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Quote:
blueyz said: had no growth, good or bad.
Quote:
MustardTiger66 said: Sounds like contam
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (12/08/13 02:29 PM)
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
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Quote:
Notahacker420 said:
Quote:
blueyz said: had no growth, good or bad.
Quote:
MustardTiger66 said: Sounds like contam

I've had contams in the center of cakes, not visible while still housed in a jar. It's a good idea to inspect/smell one regardless to rule out contam so you can move on to another diagnosis. Keep the face palm to yourself.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Quote:
Halophorm said: OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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blueyz



Registered: 11/09/13
Posts: 50
Loc: CO
Last seen: 9 years, 5 months
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Quote:
MustardTiger66 said: Was this a MS inoculation? Sounds like contam or just piss poor spores. Give them a smell? Try to to get a cake out (Outside of your grow area, of course) and give it a good inspection for contams or anything else that May have halted or prevented any growth.
Ok- Thanks for the advice. I'm not sure what I am smelling for being this is my first attempted grow. I'm not sure if I am answering your "MS inoculation" question correctly, but the first syringe was GT. I got nothing out of that and they sat for about a month. The second go around was also GT but from another supplier. This time I could physically see a ton of spores. I couldn't see any spores with the first syringe. I also have them in a thermostat controlled incubator, set to approximately 80F because without it they would be around 65F. It looks like I definitely have some growth on 1 of the jars, but I can't tell with any of the others. Should I isolate the 1 jar that has growth? Thx!
-------------------- Peace & Love
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blueyz



Registered: 11/09/13
Posts: 50
Loc: CO
Last seen: 9 years, 5 months
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Quote:
Notahacker420 said:
Quote:
Halophorm said: OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.
First inoculation was about a month ago. I got nothing. I inoculated a second go around (new substrate) about 4 days ago. I see definitive growth on only 1 of the jars.
-------------------- Peace & Love
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ImFukNCLUELESS
I SPIT ON PEOPLE


Registered: 11/19/12
Posts: 1,580
Last seen: 9 years, 19 days
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Re: Newb Question [Re: blueyz]
#19249206 - 12/09/13 09:06 AM (10 years, 1 month ago) |
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Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit
also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light 12/12 schedule and take the foil off the top
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how to pass a drug test FOO MAN'S WBS TEK damions5050's coir tek DONATE TO THE "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf "my girl said it's OK to have a little penis I prefer she didnt have a penis at all"-prisoner#1
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
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Quote:
1slow2v said: Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit
also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light 12/12 schedule and take the foil off the top
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blueyz



Registered: 11/09/13
Posts: 50
Loc: CO
Last seen: 9 years, 5 months
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Quote:
1slow2v said: Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit
also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light 12/12 schedule and take the foil off the top
Great- will do thanks. What is the purpose of taking the foil off the top though? Wouldn't that increase the risk of contams? Shouldn't their be enough room for gas exchange even with it on?
-------------------- Peace & Love
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ImFukNCLUELESS
I SPIT ON PEOPLE


Registered: 11/19/12
Posts: 1,580
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Re: Newb Question [Re: blueyz]
#19249409 - 12/09/13 10:20 AM (10 years, 1 month ago) |
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No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth
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how to pass a drug test FOO MAN'S WBS TEK damions5050's coir tek DONATE TO THE "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf "my girl said it's OK to have a little penis I prefer she didnt have a penis at all"-prisoner#1
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
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Quote:
1slow2v said: No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth
I made this mistake on my first grow. Take that shit off. It's only purpose is to protect the top of your jar during PC.
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TheWiz
Happy Little Shroom



Registered: 11/21/11
Posts: 191
Loc: Southern IL
Last seen: 3 years, 4 months
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Quote:
MustardTiger66 said:
Quote:
Notahacker420 said:
Quote:
blueyz said: had no growth, good or bad.
Quote:
MustardTiger66 said: Sounds like contam

I've had contams in the center of cakes, not visible while still housed in a jar. It's a good idea to inspect/smell one regardless to rule out contam so you can move on to another diagnosis. Keep the face palm to yourself.
Also, bacterial contams aren't always obvious and can go unnoticed by beginners.
-------------------- I'd hit it.
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blueyz



Registered: 11/09/13
Posts: 50
Loc: CO
Last seen: 9 years, 5 months
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Quote:
1slow2v said: No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth
Ahhhh, got it. Makes sense. Thanks!
-------------------- Peace & Love
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Quote:
1slow2v said: No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth
I doubt it.....the foil is not sealing it air tight.
Gasses will pass thru the path of least resistance.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Halophorm
Pinset extrordinaire



Registered: 11/25/13
Posts: 114
Last seen: 5 years, 11 months
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Quote:
1slow2v said: Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit
also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light 12/12 schedule and take the foil off the top
CORRECT!
-------------------- "Imagination is more important than knowledge." -Albert Einstien
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Halophorm
Pinset extrordinaire



Registered: 11/25/13
Posts: 114
Last seen: 5 years, 11 months
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Quote:
Notahacker420 said:
Quote:
1slow2v said: No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth
I doubt it.....the foil is not sealing it air tight.
Gasses will pass thru the path of least resistance.
even then, more GE is better than less especially because there's restricted airflow due to the incubator.
-------------------- "Imagination is more important than knowledge." -Albert Einstien
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Quote:
1slow2v said: also 80 degrees is to hot (risk of contams)
No it's not.....80F is fine, and heat does not cause contams....contams cause contams.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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